Journal of Clinical Biochemistry and Nutrition This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

5- N -Carboxyimino-6-aminopyrimidine-2,4(3 H )-dione, a novel indicator for hypochlorite formation
In this study, we found that 5-N-carboxyimino-6-N-chloroaminopyrimidine-2,4(3H)-dione rapidly reacted with thiol compounds such as cysteine and glutathione to yield 5-N-carboxyimino-6-aminopyrimidine-2,4(3H)-dione, which was stable in human plasma unlike 5-N-carboxyimino-6-N-chloroaminopyrimidine-2,4(3H)-dione. 5-N-carboxyimino-6-aminopyrimidine-2,4(3H)-dione was produced upon uric acid degradation during myeloperoxidase-induced uric acid oxidation and lipopolysaccharide-induced pseudo-inflammation in collected 2,4(3H)-dione has potential as a marker for hypochlorite production in vivo.PMID:38510685 | PMC:PMC10948346 | DOI...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Aya Matsubara Haruki Tanno Sakiko Amekura Sayaka Iida Yorihiro Yamamoto Akio Fujisawa Source Type: research

Crosstalk between NOD2 and TLR2 suppresses the development of TLR2-mediated experimental colitis
J Clin Biochem Nutr. 2024 Mar;74(2):146-153. doi: 10.3164/jcbn.23-87. Epub 2023 Dec 15.ABSTRACTNucleotide-binding oligomerization domain 2 (NOD2) is an intracellular sensor for muramyl dipeptide (MDP), a degradation product of bacterial cell wall peptidoglycan (PGN). PGN stimulates cell-surface Toll-like receptor 2 (TLR2) independently of NOD2, indicating the presence of crosstalk between extracellular TLR2 and intracellular NOD2 upon exposure to PGN. NOD2-deficient mice were sensitive, while TLR2-deficient mice were resistant to experimental colitis induced by intrarectal administration of PGN. Severe colitis in NOD2-defi...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Natsuki Okai Yasuhiro Masuta Yasuo Otsuka Akane Hara Sho Masaki Ken Kamata Kosuke Minaga Hajime Honjo Masatoshi Kudo Tomohiro Watanabe Source Type: research

Cytokine and chemokine profiles in ulcerative colitis relapse after coronavirus disease 2019 vaccination
J Clin Biochem Nutr. 2024 Mar;74(2):127-135. doi: 10.3164/jcbn.23-26. Epub 2023 Sep 16.ABSTRACTCoronavirus disease 2019 (COVID-19) vaccines are highly effective; however, vaccine-related adverse events, including autoimmunity, have been reported. Case reports describing relapse or new-onset of ulcerative colitis (UC) after COVID-19 mRNA vaccination are available. However, the molecular mechanisms underlying the development of colonic inflammation associated with COVID-19 mRNA vaccination are poorly understood. Furthermore, it is unclear whether the relapse of UC after COVID-19 vaccination is driven by unique cytokine respo...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Yasuhiro Masuta Kosuke Minaga Yasuo Otsuka Natsuki Okai Akane Hara Sho Masaki Tomoyuki Nagai Hajime Honjo Masatoshi Kudo Tomohiro Watanabe Source Type: research

Unique and overlapping roles of NRF2 and NRF1 in transcriptional regulation
J Clin Biochem Nutr. 2024 Mar;74(2):91-96. doi: 10.3164/jcbn.23-106. Epub 2023 Nov 22.ABSTRACTTranscription is regulated by specific transcription factors that mediate signaling in response to extrinsic and intrinsic stimuli such as nutrients, hormones, and oxidative stresses. Many transcription factors are grouped based on their highly conserved DNA binding domains. Consequently, transcription factors within the same family often exhibit functional redundancy and compensation. NRF2 (NFE2L2) and NRF1 (NFE2L1) belong to the CNC family transcription factors, which are responsible for various stress responses. Although their ...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Hiroki Sekine Hozumi Motohashi Source Type: research

High-intensity exercise impairs intestinal barrier function by generating oxidative stress
In this study, we examined the involvement of oxidative stress in barrier function of the ileum of mice following high-intensity exercise. Male ICR mice (12-week-old) were divided into sedentary and exercise groups. Mice in the exercise group underwent a single bout of treadmill running, and the ileum was collected for histological and biochemical analyses. Plasma fluorescence intensity level after oral administration of fluorescein isothiocyanate-dextran gradually increased until 30 min after exercise in response to intensity of exercise. Relatively high levels of oxidative proteins and low level of claudin-1, a tight-jun...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Maki Takami Wataru Aoi Karin Matsumoto Yoji Kato Yukiko Kobayashi Masashi Kuwahata Source Type: research

Prosaposin is a novel coenzyme Q10-binding protein
J Clin Biochem Nutr. 2024 Mar;74(2):108-112. doi: 10.3164/jcbn.23-57. Epub 2023 Nov 8.ABSTRACTCoenzyme Q10 (CoQ10) is essential for mitochondrial ATP production and functions as an important antioxidant in every biomembrane and lipoprotein. Due to its hydrophobicity, a binding and transfer protein for CoQ10 is plausible, and we previously described saposin B as a CoQ10-binding and transfer protein. Here, we report that prosaposin, the precursor of saposin B, also binds CoQ10. As prosaposin is both a secretory protein and integral membrane protein, it is ubiquitous in the body. Prosaposin was isolated from human seminal pla...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Makoto Hasegawa Yorihiro Yamamoto Akio Fujisawa Misato Kashiba Source Type: research

Slowly progressive cell death induced by GPx4-deficiency occurs via MEK1/ERK2 activation as a downstream signal after iron-independent lipid peroxidation
In this study, we established Tamoxifen-inducible GPx4-deficient Mouse embryonic fibroblast (MEF) cells (ETK1 cells) and found that Tamoxifen-inducible gene disruption of GPx4 induces slow cell death at ~72 h. In contrast, RSL3- or erastin-induced ferroptosis occurred quickly within 24 h. Therefore, we investigated the differences in these mechanisms between GPx4 gene disruption-induced cell death and RSL3- or erastin-induced ferroptosis. We found that GPx4-deficiency induced lipid peroxidation at 24 h in Tamoxifen-treated ETK1 cells, which was not suppressed by iron chelators, although lipid peroxidation in RSL3- or erast...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Kahori Tsuruta Masaki Matsuoka Shinsaku Harada Ayaka Enomoto Takeshi Kumagai Shu Yasuda Tomoko Koumura Ken-Ichi Yamada Hirotaka Imai Source Type: research

Improvement trend for individual health guidance intervention according to Japan clinical guidelines by public health nurses for type 2 diabetes mellitus who visited for medical checkups regularly: a case-control preliminary report
In conclusion, the implementation of individual health guidance interventions may prove to be effective for individuals with type 2 diabetes mellitus and obesity who regularly attend medical checkups.PMID:38510680 | PMC:PMC10948351 | DOI:10.3164/jcbn.23-52 (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Rina Matsuo Tomohiro Imamura Ayako Takamori Takuya Kishi Miwako Minami Junko Miyakawa Natsuki Yoshitake Ai Hayashi Yuki Nakayama Natsumi Egashira Minako Teramoto Hiroko Ishinari Itsuka Kajiyama Satomi Fujisaki Hitomi Kakiyama Kanako Satou Rei Nakafusa Chi Source Type: research

A mixed antioxidant supplement improves cognitive function, and coordination in aged mice
In this study, aged mice were fed the combination supplement and cognitive and motor functions were measured. Following 1 month of treatment with the supplement, significant improvements in cognitive function and neuromuscular coordination were observed. Following 2 weeks of treadmill training, treatment with the supplement dramatically increased running distance compared to that in untreated normal aged mice. Serum indices such as triglyceride and total cholesterol were significantly decreased in the supplement-treated aged mice compared to untreated aged mice. These results indicate that the combination supplement may pl...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Koji Fukui Fukka You Yugo Kato Shuya Yuzawa Ayuta Kishimoto Takuma Hara Yuki Kanome Yoshiaki Harakawa Toshikazu Yoshikawa Haruhiko Inufusa Source Type: research

Clinical significance of CDT1 mRNA expression in chronic hepatitis C or liver cirrhosis
We examined the association between the CDT1 mRNA expression and clinical conditions and long-term outcome. We then examined the association between serum cytokine/chemokine levels and CDT1 mRNA expression in 58 cases. The cumulative incidence rates of HCC development in cases with CDT1 mRNA in the low expression group showed significantly lower than those in the high expression group (p = 0.0391). A significant correlation was found between CDT1 mRNA expression and the extent of proliferation of atypical hepatocytes in hematoxylin and eosin-stained sections (p<0.0001). CDT1 mRNA expression has been associated with cyto...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Masahiro Ogawa Mitsuhiko Moriyama Hitomi Nakamura Toshikatu Shibata Kazumichi Kuroda Masahiko Sugitani Source Type: research

Zinc supplementation with polaprezinc was associated with improvements in albumin, prothrombin time activity, and hemoglobin in chronic liver disease
In conclusion, serum zinc levels were correlated with serum Alb, Hb, and PT% in patients with CLD, and zinc supplementation with polaprezinc was associated with improvements in Alb, Hb, and PT% within at least 6 months.PMID:38510683 | PMC:PMC10948345 | DOI:10.3164/jcbn.23-89 (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Seigo Abiru Yuki Kugiyama Tomoyuki Suehiro Yasuhide Motoyoshi Akira Saeki Shinya Nagaoka Kazumi Yamasaki Atsumasa Komori Hiroshi Yatsuhashi Source Type: research

Can bone mass measured via bioelectrical impedance analysis be used to diagnose sarcopenia?
This study aimed to evaluate the relationship between sarcopenia and bone mass [measured by bioelectrical impedance analysis (BIA)], as well as to identify new indicators associated with this disease. Basic interviews and measurement of physical function were performed on 474 community-dwelling older adults (aged 77.1 ± 7.6 years), including older adult patients with sarcopenia, in Wakasa Town, Fukui Prefecture. The findings led to 363, 71, and 40 participants being classified as 'normal', 'pre-sarcopenia', and 'having sarcopenia', respectively. An Ordinal Logistic Regression Analysis showed that age, bone mass phase angl...
Source: Journal of Clinical Biochemistry and Nutrition - March 21, 2024 Category: Nutrition Authors: Naohiro Konoshita Hidenori Onishi Yasutaka Mizukami Ryouko Ikeda Tokuharu Tanaka Hiromasa Tsubouchi Takahiro Kishimoto Hiroyuki Hayashi Osamu Yamamura Source Type: research