Reactive oxygen species in cardiovascular health and disease: special references to nitric oxide, hydrogen peroxide, and Rho-kinase.
Authors: Shimokawa H Abstract The interaction between endothelial cells and vascular smooth muscle cells (VSMC) plays an important role in regulating cardiovascular homeostasis. Endothelial cells synthesize and release endothelium-derived relaxing factors (EDRFs), including vasodilator prostaglandins, nitric oxide (NO), and endothelium-dependent hyperpolarization (EDH) factors. Importantly, the contribution of EDRFs to endothelium-dependent vasodilatation markedly varies in a vessel size-dependent manner; NO mainly mediates vasodilatation of relatively large vessels, while EDH factors in small resistance vessels. W...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effect of astaxanthin-rich extract derived from Paracoccus carotinifaciens on the status of stress and sleep in adults.
This study investigated the effect of a dietary supplement containing astaxanthin-rich extract derived from Paracoccus carotinifaciens (astaxanthin supplement) on the status of stress and sleep in individuals aged 20-64 years. Twenty-five subjects orally administered 12 mg astaxanthin/day of astaxanthin supplement for 8 weeks (astaxanthin group) and 29 subjects given a placebo (placebo group) were evaluated with Profile of Mood States 2nd Edition for stress and Oguri-Shirakawa-Azumi Sleep Inventory for Middle-aged and Aged version for sleep. We did not observe any significant intergroup differences in the stress and s...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1 β via a c-Jun N-terminal kinase pathway.
Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1β via a c-Jun N-terminal kinase pathway. J Clin Biochem Nutr. 2020 Mar;66(2):103-109 Authors: Fukumoto M, Takeuchi T, Koubayashi E, Harada S, Ota K, Kojima Y, Higuchi K Abstract Brain-derived neurotrophic factor exhibits neurotropic and neuroprotective functions and is increased in the colonic mucosa of patients with irritable bowel syndrome in correlation with the severity and frequency of abdominal pain. However, there are no reports of brain-derived neurotrophic factor expression in enteric glial ce...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Lysyl oxidase expression is regulated by the H3K27 demethylase Jmjd3 in tumor-associated M2-like macrophages.
Authors: Takemoto R, Kamiya T, Hara H, Adachi T Abstract Copper is one of the essential micronutrients, and copper-containing enzymes contribute to crucial functions in the body. Lysyl oxidase is a copper-containing enzyme that remodels the extracellular matrix by cross-linking collagen and elastin. The overexpression of lysyl oxidase was recently shown to promote tumor metastasis. M2-like macrophages were also found to significantly accumulate in the tumor microenvironment, and correlated with a poor patient's outcome. We speculate that M2-like macrophages promote tumor progression via lysyl oxidase expression. Ep...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Inhibition of lipid peroxidation during the reproductive period extends the lifespan of Caenorhabditis elegans.
Authors: Sakamoto T, Maebayashi K, Tsunoda Y, Imai H Abstract Glutathione peroxidase 4 (GPx4) is a unique antioxidant enzyme that directly reduces the phospholipid hydroperoxides (PLOOH) generated in biomembranes using glutathione as the reductant. We have previously reported that the Caenorhabditis elegans gpx-quad mutant, which lacks all homologous genes of GPx4 has a reduced lifespan compared with the wild-type. However, the mechanisms underlying the lifespan reduction remain unclear. By monitoring the change in PLOOH production with age, we found that PLOOH was elevated in the gpx-quad mutants compared with the...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effects of exercise on biological trace element concentrations and selenoprotein P expression in rats with fructose-induced glucose intolerance.
Authors: Miya N, Uratani A, Chikamoto K, Naito Y, Terao K, Yoshikawa Y, Yasui H Abstract In the present study, we investigated the effects of exercise intended to prevent or treat lifestyle-related diseases on the glucose tolerance, insulin level, lactic acid utilization, muscle glycogen synthesis, hepatic and renal oxidative stress, hepatic selenoprotein P and biological trace element levels in organs of obese, glucose-intolerant rats. We fed normal, healthy rats a 20% casein diet while the glucose-intolerant, obese rats received a high-fructose diet. They were forced to run for one hour per day, six days per week...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Hypoxia and autophagic response of obese adult rat adipocytes which differ in nutritional state during childhood.
This study aims to analyse the difference in the size and number of visceral adipocytes, HIF-1α, HIF-2α and MAP1LC3A/LC3 in obese adult rat groups that were undernourished at a young age compared to groups who were normal or even already fat from childhood. We analyzed Hif-1α, Hif-2α, Lc3 mRNA by RT-qPCR; HIF-1α, HIF-2α, MAP1LC3A/LC3 protein level by ELISA. The HIF-1α and HIF-2α protein level of visceral adipocytes derived from the group of rat which were undernourished while young increased significantly compared to the group which was overnourished. The visceral adipocytes ...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effects of repetitive diet-induced fluctuations in plasma phosphorus on vascular calcification and inflammation in rats with early-stage chronic kidney disease.
Authors: Tani M, Tanaka S, Takamiya K, Sakaue M, Ito M Abstract Cardiovascular disease is a major cause of death among hemodialysis patients. Hyperphosphatemia induces cardiovascular disease through vascular endothelial dysfunction and calcification. Repetition of a short-term excessive-phosphorus (P) diet causes transient elevations in plasma P and subsequent vascular endothelial dysfunction in normal rats. The purpose of this study was to investigate the effects of the P fluctuation on vascular calcification and inflammation in rats after unilateral nephrectomy as an early-stage chronic kidney disease (CKD) model...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effect of fish oil supplementation combined with high-intensity interval training in newly diagnosed non-obese type 2 diabetes: a randomized controlled trial.
Authors: Hua L, Lei M, Xue S, Li X, Li S, Xie Q Abstract The additive effect of high-intensity interval training to fish oil supplementation on newly diagnosed type 2 diabetes is unknown. 173 newly diagnosed type 2 diabetes patients were randomly assigned into the control group (received corn oil), fish oil group (eicosapentaenoic acid, EPA:docosahexaenoic acid, DHA = 3:2, total 2.0 g/day), and the fish oil + high-intensity interval training group. Three instructed high-intensity interval training sessions (Monday, Wednesday, and Friday; 10 × 60-s cycling bouts) were perform...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

The association among enterobacterial flora, dietary factors, and prognosis in patients with ulcerative colitis.
Authors: Sugiyama T, Sasaki M, Nakagawa S, Inoue S, Adachi K, Yoshimine T, Yamaguchi Y, Tamura Y, Izawa S, Hijikata Y, Ebi M, Mizuno M, Yamamoto S, Funaki Y, Ogasawara N, Goto C, Kasugai K Abstract The role of enterobacterial flora in the onset and progression of inflammatory bowel diseases is a topic of considerable interest. Here, we assessed the association among enterobacterial flora, dietary factors, and ulcerative colitis (UC) progression. Forty-six patients with UC who were diagnosed as being in remission were enrolled. We collected each patient's stool sample one or two days before diagnostic colonoscopy. A...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Current states of prevention of drug-induced gastroduodenal ulcer in real clinical practice: a cross-sectional study.
Authors: Iwamoto J, Murakami M, Monma T, Ueda H, Tamamushi M, Konishi N, Yara SI, Hirayama T, Ikegami T, Honda A, Mizokami Y Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) or low-dose aspirin (LDA) are the most common causes of drug-induced gastroduodenal ulcer and We investigated preventive treatment with use of concomitant anti-ulcer drugs and the clinical features of gastroduodenal ulcer in cases treated with these drugs. Patients with gastroduodenal ulcer and patients with bleeding were classified into 3 groups: LDA, non-aspirin NSAIDs, and those taking neither aspirin nor NSAIDs. Chronological changes...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Relationship between dietary inflammatory index, hs-CRP level in the second trimester and neonatal birth weight: a cohort study.
Authors: Yang Y, Kan H, Yu X, Yang Y, Li L, Zhao M Abstract The aim of this study was to investigate whether diet plays a role in the effect of inflammation on birth weight. The normal pre-pregnancy body mass index and healthy single pregnant women without classical inflammatory were recruited at 16-20 weeks of pregnancy and provided blood sample to measure plasma high sensitive C-reactive protein (hs-CRP) level. The Dietary Inflammatory Index (DII) score was calculated by a three-day 24 h recall method, and a cohort of 307 eligible pregnant women was established. According to birth weight, the subjects were d...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Selenoprotein P as an in vivo redox regulator: disorders related to its deficiency and excess.
Authors: Saito Y Abstract Selenoprotein P (encoded by SELENOP) contains the essential trace element selenium in the form of selenocysteine, which is an analog of cysteine that contains selenium instead of sulfur. Selenoprotein P is a major selenium-containing protein in human plasma and is mainly synthesized in the liver. It functions as a selenium-transporter to maintain antioxidative selenoenzymes in several tissues, such as the brain and testis, and plays a pivotal role in selenium-metabolism and antioxidative defense. A decrease of selenoprotein P and selenoproteins causes various dysfunctions related to oxidat...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Trapa bispinosa Roxb. and lutein ameliorate cataract in type 1 diabetic rats.
Authors: Kinoshita S, Sugawa H, Nanri T, Ohno RI, Shirakawa JI, Sato H, Katsuta N, Sakake S, Nagai R Abstract Trapa bispinosa Roxb. is an annual aquatic grass of the citrus family. Although its hot water extract displays antioxidative activity in vitro, little is known about its biological effectiveness. In the present study, we evaluated the extract's inhibitory effect on diabetic cataractogenesis and formation of advanced glycation end-product. Lutein, which is beneficial for eye diseases, was administered concurrently. For short-term administration, Trapa bispinosa Roxb. hot water extract and/or lutein were admi...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells.
Authors: Takishita Y, Yasuda H, Shimizu M, Matsuo A, Morita A, Tsutsumi T, Tsuchiya M, Sato EF Abstract Neutrophil extracellular trap (NET) formation plays an important role in inflammatory diseases. Although it is known that NET formation occurs via NADPH oxidase (NOX)-dependent and NOX-independent pathways, the detailed mechanism remains unknown. Therefore, in this study, we aimed to elucidate the role of mitochondria in NOX-dependent and NOX-independent NET formation. We generated mitochondrial DNA-deficient cells (ρ0 cells) by treating HL-60 cells with dideoxycytidine and differentiated them to neutrophil-l...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Supplementation with nitrate only modestly affects lipid and glucose metabolism in genetic and dietary-induced murine models of obesity.
Authors: Fischer A, Lüersen K, Schultheiß G, de Pascual-Teresa S, Mereu A, Ipharraguerre IR, Rimbach G Abstract To gain a better understanding of how nitrate may affect carbohydrate and lipid metabolism, female wild-type mice were fed a high-fat, high-fructose diet supplemented with either 0, 400, or 800 mg nitrate/kg diet for 28 days. Additionally, obese female db/db mice were fed a 5% fat diet supplemented with the same levels and source of nitrate. Nitrate decreased the sodium-dependent uptake of glucose by ileal mucosa in wild-type mice. Moreover, nitrate significantly decreased triglyceride con...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication.
This study was conducted to evaluate the regulation mechanism of influenza virus replication following treatment of Madin-Darby canine kidney cells with the soy isoflavone daidzein. We performed comparative qualitative and quantitative analyses of lipid peroxide between mock-infected and virus-infected cells treated with or without daidzein, as it had been reported that daidzein was an antioxidant and lipid peroxide levels increased upon virus infection. Contrary to our belief, lipid peroxides were not elevated in virus-infected cells and no decrease in lipid peroxides was observed in daidzein-treated cells. In daidzein-tr...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

The effects of isoflavone supplementation plus combined exercise on salivary markers of oxidative stress in postmenopausal women.
This study tested the effect of isoflavone supplementation in addition to combined exercise training in salivary oxidative stress markers in non-obese postmenopausal women. Thirty-two postmenopausal women without hormone therapy were randomly assigned to exercise + placebo (n = 15) or exercise + isoflavone supplementation (n = 17) groups. They performed 30 sessions of combined exercises (aerobic plus resistance) over ten weeks and consumed 100 mg of isoflavone supplementation or placebo. Saliva samples were collected after an overnight fast. Superoxide dismutase, total antioxida...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Association of increased renal Cyp24a1 gene expression with low plasma 1,25-dihydroxyvitamin D levels in rats with streptozotocin-induced diabetes.
Authors: Tajiri M, Nakahashi O, Kagawa T, Masuda M, Ohminami H, Iwano M, Takeda E, Taketani Y, Yamamoto H Abstract Decreases in plasma vitamin D concentrations have been reported in diabetes, although the mechanism involved in this decrease is unclear. Here, we investigated the association between Cyp24a1, a vitamin D catabolic enzyme, and abnormalities in vitamin D metabolism in streptozotocin-induced diabetes rats, an animal model of type 1 diabetes. Plasma 1,25-dihydroxyvitamin D [1,25(OH)2D] levels were significantly lower in streptozotocin-induced diabetes rats and renal Cyp24a1 mRNA expression levels were inc...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Salivary 8-hydroxyguanine as a lifestyle-related oxidative stress biomarker in workers.
Authors: Watanabe S, Kawasaki Y, Kawai K Abstract Oxidative stress is a risk factor for lifestyle-related diseases, such as cancer. Investigations of the factors that increase or decrease oxidative stress contribute to disease prevention. In the present study, we focused on the 8-hydroxyguanine (8-OHGua) in saliva, as a new oxidative stress biomarker. The relationship between lifestyles and salivary 8-OHGua levels in 541 Japanese subjects was analyzed. The salivary 8-OHGua levels were significantly elevated in older persons, as well as those who smoke, have hypertension, or excess visceral fat. By contrast, statist...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Combined aerobic and low-intensity resistance exercise training increases basal nitric oxide production and decreases arterial stiffness in healthy older adults.
Authors: Otsuki T, Namatame H, Yoshikawa T, Zempo-Miyaki A Abstract Meta-analyses have concluded that combined aerobic and high-intensity or moderate-intensity resistance exercise training has no effects on arterial stiffness. However, a recent study demonstrated that combined aerobic training and resistance training using rubber bands increases basal nitric oxide (NO) production and decreases arterial stiffness with marked reduction of body weight in obese adolescent girls. To investigate whether combined aerobic and low-intensity resistance training increases basal NO production and decreases arterial stiffness w...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

The effect of preoperative synbiotic treatment to prevent surgical-site infection in hepatic resection.
Authors: Iida H, Sasaki M, Maehira H, Mori H, Yasukawa D, Takebayashi K, Kurihara M, Bamba S, Tani M Abstract We aimed to clarify the influence of preoperative synbiotic therapy on surgical-site infections (SSIs) after hepatic resection. Between January 2011 and December 2017, 284 patients who underwent hepatic resection without biliary tract reconstruction and resection of other organs were included. We prospectively administered Clostridium butyricum and partially hydrolyzed guar gum before hepatic resection between April 2016 and December 2017 (synbiotic group). One-hundred-fifteen patients of the synbiotic grou...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Estimation of salt intake from spot urine may assist the risk assessment of gastric cancer.
In conclusion, salt intake estimated from spot urine was high in patients with gastric cancer, especially in H. pylori infected patients. Spot urine is a simple examination and it may be applied as a new risk assessment of gastric cancer. PMID: 32001960 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Adverse events associated with bidirectional endoscopy with midazolam and pethidine.
Authors: Nishizawa T, Sakitani K, Suzuki H, Takeuchi M, Takahashi Y, Takeuchi K, Yamakawa T, Yoshida S, Hata K, Ebinuma H, Koike K, Toyoshima O Abstract Same-day bidirectional endoscopy has been reported to reduce recovery time, and procedure-related cost. The safety of bidirectional endoscopy vs colonoscopy only, while using midazolam and pethidine, has never been evaluated. We reviewed 1,202 consecutive patients who underwent bidirectional endoscopy or colonoscopy only with administration of midazolam and pethidine in Toyoshima Ensdoscopy Clinic. We compared the clinical characteristics and adverse events associa...
Source: Journal of Clinical Biochemistry and Nutrition - February 1, 2020 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Measurement of homocysteine: a historical perspective.
In conclusion, we have presented the historical perspective of homocysteine measurement in biological fluids in this manuscript. Thus, the precise understanding of its concentration in biological fluids coupled with its importance in health and disease should justify a newer but reliable technique in the area of ongoing research in homocysteine. PMID: 31777417 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Dendrimer porphyrins as the oxygen sensor for intracellular imaging to suppress interaction towards biological molecules.
Authors: Odai S, Ito H, Kamachi T Abstract Optical methods using phosphorescence quenching by oxygen are suitable for the measurement of oxygen concentration within cells. In cells, however, the dyes such as Pt-porphyrins interact with biological components so that their optical properties are changed. Therefore, the absolute oxygen concentration determination in cells is difficult. To suppress this interaction, we focussed on porphyrin-cored dendrimers (dendrimer-porphyrins) and synthesized 2nd-4th generation dendrimer-porphyrins with various surface functional groups (G2-G4, ARG, αGLU and γGLU). These...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Reduction of molecular oxygen by redox active thiols: comparison of glutathione, N-acetylcysteine, cysteine, and homocysteine.
Authors: Nyui M, Shoji Y, Ueno M, Nakanishi I, Matsumoto KI Abstract The reaction properties of the thiol compounds, cysteine (Cys), N-acetyl-l-cysteine (NAC), the reduced form glutathione (GSH), and homocysteine (HCS) were compared. The main purpose of this study was to find a thiol-based anti-oxidant suitable for biological experiments and to provide clear reasoning for its selection. The availability of thiol compounds to generate superoxide by reducing molecular oxygen (O2) at a hyperthermal temperature was discussed. An oxidative atmosphere, i.e., superoxide generation by the hypoxanthine-xanthine oxidase reac...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

A new insight into the apoptotic effect of nitidine chloride targeting Checkpoint kinase 2 in human cervical cancer in vitro.
Authors: Kwon HJ, Kim LH, Ahn CH, Yang IH, Hong KO, Doo Hong S, Shin JA, Cho SD Abstract Nitidine chloride (NC), a natural, bioactive, phytochemical alkaloid derived from the roots of Zanthoxylum nitidum, has been reported to exhibit anti-tumor activity against various types of cancer. However, the potential therapeutic role of NC in human cervical cancer has not yet been studied. We are the first to report that NC acts as a potential apoptosis-inducing agent for human cervical cancer in vitro. NC treatment of human cervical cancer cell lines induced caspase-mediated apoptosis, thereby reducing cell viability. Phos...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Activation of NF-E2 p45-related factor-2 transcription and inhibition of intestinal tumor development by AHCC, a standardized extract of cultured Lentinula edodes mycelia.
Authors: Takahashi M, Fujii G, Hamoya T, Kurokawa Y, Matsuzawa Y, Miki K, Komiya M, Narita T, Mutoh M Abstract It has been reported that activation of NF-E2 p45-related factor-2 (NRF2), a transcription factor, induces a variety of antioxidant enzymes, and plays an important role in preventing carcinogenesis. AHCC is a standardized extract of cultured Lentinula edodes mycelia and it has been demonstrated to improve cancer. However, the effects of AHCC on NRF2 have not been examined, and the effects on intestinal adenoma development are not yet fully understood. We first investigated the effects of AHCC (1-5 mg/...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Ascorbic acid insufficiency impairs spatial memory formation in juvenile AKR1A-knockout mice.
Authors: Kurihara K, Homma T, Kobayashi S, Shichiri M, Fujiwara H, Fujii S, Yamada KI, Nakane M, Kawamae K, Fujii J Abstract AKR1A, an aldo-keto reductase, is involved in the synthesis of ascorbic acid as well as the reduction of a variety of aldehyde compounds. AKR1A-/- mice produce considerably less ascorbic acid (about 10%) compared to AKR1A+/+ mice and require ascorbic acid supplementation in order to breed. To elucidate the roles played by AKR1A in spatial memory, AKR1A-/- male mice were weaned at 4 weeks of age and groups that received ascorbic acid supplementation and no supplementation were subjected to a M...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Direct assessment of the antioxidant property of salivary histatin.
In this study, the effects of histatin on direct reactive oxygen species scavenging activity were examined using electron spin resonance. We demonstrated, for the first time, that histatin exhibits antioxidant activity against hydroxyl radicals generated by Fenton's reaction by metal chelation or binding. The direct antioxidant effects of histatin, along with its antimicrobial activity, may be important in the oral protection of salivary proteins. PMID: 31777423 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Moderate intake of aspartame and sucralose with meals, but not fructose, does not exacerbate energy and glucose metabolism in estrogen-deficient rats.
In conclusion, aspartame and sucralose with a meal might be preferable sweeteners to fructose and sucrose in estrogen deficient rats, and possibly post-menopausal women; however, this needs to be confirmed in human studies. PMID: 31777424 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Plasma quercetin metabolites are affected by intestinal microbiota of human microbiota-associated mice fed with a quercetin-containing diet.
This study indicates that intestinal microbiota of human microbiota-associated mice inoculated with different fecal suspensions react to dietary quercetin in different ways. PMID: 31777425 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Analysis of gut microbiota in patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Authors: Matsuura J, Inoue R, Takagi T, Wada S, Watanabe A, Koizumi T, Mukai M, Mizuta I, Naito Y, Mizuno T Abstract Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a major hereditary small vessel disease caused by mutations in NOTCH3. The variations in progression and severity among patients suggest that the CADASIL phenotype is modified by some genetic and environmental factors. Recent studies have shown the potential roles of gut microbiota in human diseases. We hypothesized that gut microbiota modifies the disease phenotype. We performed gut microbial meta...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Pulmonary activation of vitamin D3 and preventive effect against interstitial pneumonia.
Authors: Tsujino I, Ushikoshi-Nakayama R, Yamazaki T, Matsumoto N, Saito I Abstract Calcitriol [1,25(OH)2D3] is usually investigated in studies on the preventive effect of activated vitamin D against interstitial pneumonia. Although cholecalciferol (vitamin D3) can be easily obtained in the diet and has a longer half-life than calcitriol, there have been few investigations of its effect on interstitial pneumonia. We used human pulmonary fibroblast cell lines (HPFCs) and a mouse model of bleomycin-induced pulmonary fibrosis to evaluate whether vitamin D3 was activated in the lungs and had a preventive effect against...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effects of dietary salt restriction on home blood pressure in diabetic patients with excessive salt intake: a pilot study.
Authors: Ushigome E, Oyabu C, Iwai K, Kitagawa N, Kitae A, Kimura T, Yokota I, Ushigome H, Hamaguchi M, Asano M, Yamazaki M, Fukui M Abstract The aim of the present study was to examine whether dietary salt restriction guidance is beneficial for dietary salt restriction and lowering of home blood pressure in patients with diabetes with excessive salt intake. We performed an intervention trial of 37 people with type 2 diabetes and excessive salt intake. National registered dietitians provided dietary salt restriction guidance to each patient at the start of the study. All participants were instructed to perform trip...
Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.
Authors: Matsumoto H, Kuroki Y, Higashi S, Goda K, Fukushima S, Katsumoto R, Oosawa M, Murao T, Ishii M, Oka K, Takahashi M, Osaki T, Kamiya S, Shiotani A Abstract The mucosa-associated microbiota is an important component in human microbiota. The aim was to investigate mucosa-associated microbiota using brush samples during endoscopic procedures and compare with fecal microbiota. Seven patients who were planning to undergo a routine colonoscopy were recruited. Mucosal brushing samples were taken from 3 sites (terminal ileum, ascending and sigmoid colon), and a fecal sample was taken on the morning of colonoscopy. ...
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Anti-obesity effects of α-cyclodextrin-stabilized 4-methylthio-3-butenyl isothiocyanate from daikon (Raphanus sativus var. longipinnatus) in mice.
In conclusion, MTBI-αCD exerted anti-inflammation and antioxidant effects to suppress lipid accumulation in epididymal adipose tissue and the liver. These effects then prevented HFD-induced obesity in mice. PMID: 31592053 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Curcumin may induce lipolysis via proteo-stress in Huh7 human hepatoma cells.
In this study, we found that curcumin or heat shock treatment up-regulated the expression of adipose triglyceride lipase (ATGL) in Huh7 hepatoma cells, which resulted in acceleration of lipolysis. Interestingly, perturbation of protein homeostasis was seen in curcumin-treated cells, as detected by formation of numerous ubiquitinated proteins and conjugated proteins with p62 (SQSTM). Curcumin activated the protein expression of molecular chaperones, such as heat shock protein (HSP)40 and HSP70. Pre-treatment of the cells with 4-phenylbutyric acid, a chemical chaperone, suppressed proteo-stress induced by curcumin and reduce...
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Endoscopic gastric mucosal atrophy as a predictor of colorectal polyps: a large scale case-control study.
Authors: Kawahara Y, Kodama M, Mizukami K, Saito T, Hirashita Y, Sonoda A, Fukuda K, Matsunari O, Okamoto K, Ogawa R, Okimoto T, Murakami K Abstract Although some studies have indicated a correlation between Helicobacter pylori infection and the risk of colorectal neoplasms, these findings have not been consistent and are controversial. This case-control study aimed to investigate the association between endoscopic gastric mucosal atrophy and colorectal polyp occurrence. Records of 7,394 participants who underwent colonoscopy examinations from August 2008 to July 2018 were reviewed retrospectively. A total of 2,404...
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

5-Aminolevulinic acid induced apoptosis via oxidative stress in normal gastric epithelial cells.
In this study, we investigated the effect of 5-aminolevulinic acid on normal and cancer cells with regard to oxidative stress. We used the rat normal gastric cell line RGM and its cancer-like mutant cell line RGK. 5-Aminolevulinic acid treatment of RGM cells enhanced reactive oxygen species generation and induced apoptosis associated with p53, whereas RGK cells were unaffected. In addition, RGM cell viability was recovered by application of N-acetyl-l-cysteine or p53 inhibitor. These results suggest that 5-aminolevulinic acid causes oxidative stress in normal gastric cells and induces apoptosis via the p53-dependent pathwa...
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Dietary intake of probiotic kimchi ameliorated IL-6-driven cancer cachexia.
In conclusion, dietary intake of cancer preventive kimchi can be an anticipating option to ameliorate cancer cachexia via suppressive action of IL-6 accompanied with decreased muscle atrophy and lipolysis. PMID: 31592205 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Elemental diet induces alterations of the gut microbial community in mice.
In conclusion, ED caused a reduction in bacterial diversity and altered metabolic functions. PMID: 31592206 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Gut microbiota differences in elderly subjects between rural city Kyotango and urban city Kyoto: an age-gender-matched study.
In conclusion, the present findings indicate significant differences in the gut microbiota between the provincial city and urban cities at Kyoto Prefecture. These alterations in the microbiota may provide new insights to consider the relationship between longevity and gut microbiota. PMID: 31592207 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Comparison of computed tomography findings with clinical risks factors for endoscopic therapy in upper gastrointestinal bleeding cases.
Authors: Jono F, Iida H, Fujita K, Kaai M, Kanoshima K, Ohkuma K, Nonaka T, Ida T, Kusakabe A, Nakamura A, Koyama S, Nakajima A, Inamori M Abstract Several risk scoring systems exist for acute upper gastrointestinal bleeding (UGIB). The clinical Rockall score (clinical RS) and the Glasgow-Blatchford score (GBS) are major risk scores that consider only clinical data. Computed tomography (CT) findings are equivocal in non-variceal UGIB. We compared CT findings with clinical data to predict mortality, rebleeding and need for endoscopic therapy in non-variceal UGIB patients. This retrospective, single-center study incl...
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Arterial stiffness during hyperglycemia in older adults with high physical activity vs low physical activity.
Authors: Kobayashi R, Sato K, Takahashi T, Asaki K, Iwanuma S, Ohashi N, Hashiguchi T Abstract We compared arterial stiffness after glucose intake in active and inactive elderly people with impaired glucose tolerance and clarified whether physical activity was associated with arterial stiffness after ingestion of glucose. Twenty older adults with impaired glucose tolerance were analyzed in a cross-sectional design. Based on the international physical activity questionnaire, participants were divided into the active group (daily step count: 10,175.9 ± 837.8 steps/day, n = 10) or the inactive gr...
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Circulating vitamin E and cardiometabolic measures: a Mendelian randomization analysis.
Authors: Fan C, Huang T, Kong X, Zhang X, Zou Z, Xiao J Abstract Although a large body of literature reported that high intake of vitamin E played a possible role in reducing risk of cardiometabolic diseases, conflicting results were also found in some observational studies due to confounding factors. Hence, we used a Mendelian randomization study as an alternative way to examine the causality between circulating vitamin E and cardiometabolic diseases. Summary-level data were extracted from consortia and three single nucleotide polymorphisms were used as instrumental variables. Our study showed that a one-SD increa...
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Erratum.
Authors: Abstract [This corrects the article DOI: 10.3164/jcbn.18-85.]. PMID: 31592211 [PubMed - in process] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - October 10, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

A quantitative analysis of carbon-ion beam-induced reactive oxygen species and redox reactions.
Authors: Matsumoto KI, Nyui M, Ueno M, Ogawa Y, Nakanishi I Abstract The amounts of reactive oxygen species generated in aqueous samples by irradiation with X-ray or clinical carbon-ion beams were quantified. Hydroxyl radical (•OH), hydrogen peroxide (H2O2), and the total amount of oxidation reactions, which occurred mainly because of •OH and/or hydroperoxy radicals (HO2 •), were measured by electron paramagnetic resonance-based methods. •OH generation was expected to be localized on the track/range of the carbon-ion beam/X-ray, and mM and M levels of •OH generation were observed. Total &bu...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner.
In conclusion, non-thermal plasma treatment is a potential therapeutic option for oral cancer. PMID: 31379408 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research