A quantitative analysis of carbon-ion beam-induced reactive oxygen species and redox reactions.
Authors: Matsumoto KI, Nyui M, Ueno M, Ogawa Y, Nakanishi I Abstract The amounts of reactive oxygen species generated in aqueous samples by irradiation with X-ray or clinical carbon-ion beams were quantified. Hydroxyl radical (•OH), hydrogen peroxide (H2O2), and the total amount of oxidation reactions, which occurred mainly because of •OH and/or hydroperoxy radicals (HO2 •), were measured by electron paramagnetic resonance-based methods. •OH generation was expected to be localized on the track/range of the carbon-ion beam/X-ray, and mM and M levels of •OH generation were observed. Total &bu...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner.
In conclusion, non-thermal plasma treatment is a potential therapeutic option for oral cancer. PMID: 31379408 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Sublethal treatment with plasma-activated medium induces senescence-like growth arrest of A549 cells: involvement of intracellular mobile zinc.
Authors: Hara H, Kobayashi M, Shiiba M, Kamiya T, Adachi T Abstract Plasma-activated medium (PAM) is a solution produced by exposing a liquid medium to non-thermal atmospheric pressure plasma (NTAPP). A number of reactive molecules, such as reactive oxygen species and reactive nitrogen species, are contained in PAM. Therefore, exposure to high doses of PAM results in cell death. We previously demonstrated that intracellular zinc (Zn2+) serves as an important mediator in PAM-induced cell death; however, the effects of sublethal treatment with PAM on cell functions are not fully understood. In the present study, we f...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

The sodium-glucose cotransporter 2 inhibitor luseogliflozin can suppress muscle atrophy in Db/Db mice by suppressing the expression of foxo1.
Authors: Okamura T, Hashimoto Y, Osaka T, Fukuda T, Hamaguchi M, Fukui M Abstract We investigated the effect of the sodium glucose cotransporter-2 inhibitor (SGLT-2i) luseogliflozin on skeletal muscle. Eight-week-old mice were fed a standard diet or the standard diet with added luseogliflozin for 8 weeks. The mice were divided into the following four genotype/dietary groups: Db/m mice without SGLT-2i, Db/m mice with SGLT-2i inhibitor, Db/Db without SGLT-2i, and Db/Db with SGLT-2i. Among the mice with and without SGLT-2i, the ratio of soleus and plantaris muscle to body weight in the Db/Db mice was significantly low...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Corticotropin-releasing hormone is significantly upregulated in the mouse paraventricular nucleus following a single oral dose of cinnamtannin A2 as an (-)-epicatechin tetramer.
Authors: Fujii Y, Suzuki K, Adachi T, Taira S, Osakabe N Abstract Cinnamtannin A2, an (-)-epicatechin tetramer, was reported to have potent physiological activity. Cinnamtannin A2 is rarely absorbed from the gastrointestinal tract into the blood and the mechanisms of its beneficial activities are unknown. Cinnamtannin A2 reported to increase sympathetic nervous activity, which was induced by various stressors. In present study, we examined the stress response in the mouse paraventricular nucleus following a single oral dose of cinnamtannin A2 by monitoring mRNA expression of corticotropin-releasing hormone (CRH) an...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Green tea polyphenol (epigallocatechin-3-gallate) improves gut dysbiosis and serum bile acids dysregulation in high-fat diet-fed mice.
In conclusion, the present study suggests that EGCG could alter bile acid metabolism, especially taurine deconjugation, and suppress fatty liver disease by improving the intestinal luminal environment. PMID: 31379412 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effects of astaxanthin on VEGF level and antioxidation in human aqueous humor: difference by sex.
Authors: Hashimoto H, Arai K, Takahashi J, Chikuda M Abstract In our previous report, we showed the effect of astaxanthin intake on VEGF level in the aqueous humor and the relationship between VEGF level and reactive oxygen species-related parameters and other relevant factors. VEGF level is associated with total hydroperoxide level, and a multivariate analysis identified sex as a secondary factor affecting these relationships. Here, we analyzed the effects of astaxanthin on the relationship between VEGF level and reactive oxygen species-related parameters by sex. Patients (16 males and 19 females, aged 71.3 and 70...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effect of amino-acid intake on physical conditions and skin state: a randomized, double-blind, placebo-controlled, crossover trial.
In conclusion, the young adult women having no fitness habit showed the significant increase of the muscle amount and improvement tendency of the skin texture by the continuous intake of the amino-acid supplement. PMID: 31379414 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Utility of hemoglobin A1c in detecting risk of type 2 diabetes: comparison of hemoglobin A1c with other biomarkers.
Authors: Umeno A, Yoshida Y Abstract We have previously reported that the risk of type 2 diabetes, early impaired glucose tolerance, and insulin resistance can be predicted using fasting levels of adiponectin, leptin, and insulin. Here, we aimed to evaluate the utility of hemoglobin A1c in detecting the risk of type 2 diabetes compared with other well-known biomarkers. We randomly enrolled 207 volunteers with no history of diseases, who underwent 75-g oral glucose tolerance tests and were stratified into normal, borderline, abnormal, or diabetic groups. Eighty-one participants with normal baseline levels of hemoglobin A1c (
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Acylated ghrelin levels were associated with depressive status, physical quality of life, endoscopic findings based on Kyoto classification in Japan.
Authors: Agawa S, Futagami S, Yamawaki H, Ikeda G, Noda H, Kirita K, Higuchi K, Murakami M, Kodaka Y, Ueki N, Akamizu T, Iwakiri K Abstract BACKGROUND AND AIMS: To determine whether serum acylated ghrelin levels were associated with anxiety, clinical symptoms, depressive status, quality of life, gastric motility and endoscopic findings based on Kyoto classification in functional dyspepsia (FD) patients. METHODS: We enrolled three groups, FD patients (n = 15) with high levels of acylated ghrelin, FD patients (n = 33) with normal levels of acylated ghrelin and FD patients (n = 35) wi...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Differences in Helicobacter pylori and CagA antibody changes after eradication between subjects developing and not developing gastric cancer.
Authors: Kodama M, Okimoto T, Mizukami K, Fukuda K, Ogawa R, Okamoto K, Matsunari O, Kawahara Y, Hirashita Y, Murakami K Abstract We evaluated serological Helicobacter pylori and cytotoxin-associated gene A (CagA) antibodies and endoscopic atrophy after eradication to identify factors predicting post-eradication gastric cancer development. Thirty-five patients with successful eradiation were divided into the post-eradication gastric cancer (13 cases) and non-gastric cancer (22 cases) groups. Serum Helicobacter pylori and CagA antibody titers and endoscopic atrophy before and six years after eradication were examine...
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Clinical features of hypermagnesemia in patients with functional constipation taking daily magnesium oxide.
In conclusion, renal function and magnesium oxide dosage, but not age, were associated with hypermagnesemia and high serum magnesium concentration in patients with functional constipation taking daily magnesium oxide. PMID: 31379418 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - August 7, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Formation of reactive oxygen species by irradiation of cold atmospheric pressure plasma jet to water depends on the irradiation distance.
Authors: Anzai K, Aoki T, Koshimizu S, Takaya R, Tsuchida K, Takajo T Abstract Because application of cold atmospheric pressure plasma jet (CAPPJ) to biological samples have taken large attentions, it is important to examine the effects of various CAPPJ parameters on the generation of reactive species. Here, we investigated the generation of reactive species in water by CAPPJ irradiation by changing the following parameters: irradiation time, sample volume, and irradiation distance between the sample surface and plasma jet tip. We measured 1) change in the ESR signal intensity of 4-hydroxy-2,2,6,6-tetrametylpeperid...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

High-glucose administration induces glucose intolerance in mice: a critical role of toll-like receptor 4.
Authors: Zhan X, Wang L, Wang Z, Chai S, Zhu X, Ren W, Chang X Abstract Glucose converted from a diet has been considered a high-risk factor of type 2 diabetes mellitus (T2DM). However, it is not clear how it increases the risk of T2DM. Here, we investigated the effect of high-glucose administration on glucose tolerence in wild-type and toll-like receptor 4 (TLR4) knockout mice. Mice were intragastrically administered with high-glucose. The level of fasting blood glucose, insulin and intraperitoneal glucose tolerance were measured, and insulinogenic index and HOMA-IR were calculated at 1 week. To understand mechani...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

A ketogenic diet improves the prognosis in a mouse model of peritoneal dissemination without tumor regression.
Authors: Kasumi E, Sato N Abstract Peritoneal dissemination describes a state where tumor cells spread to the surface of the peritoneum and become engrafted. Peritoneal dissemination reduces the quality of life and prognosis of cancer patients. Currently, there are few effective therapies or preventative treatments for peritoneal dissemination. The aim of this study was to evaluate a ketogenic diet, characterized by high fat, moderate protein and low carbohydrate content, as a novel therapy in a mouse model of peritoneal dissemination. BALB/c mice were intraperitoneally inoculated with colon 26, a murine colon aden...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

NUPR1 acts as a pro-survival factor in human bone marrow-derived mesenchymal stem cells and is induced by the hypoxia mimetic reagent deferoxamine.
Authors: Matsunaga K, Fujisawa K, Takami T, Burganova G, Sasai N, Matsumoto T, Yamamoto N, Sakaida I Abstract Differences in the culturing conditions of mesenchymal stem cells used in regenerative medicine may affect their differentiation ability, genome instability, and therapeutic effects. In particular, bone marrow-derived mesenchymal stem cells cultured under hypoxia are known to proliferate while maintaining an undifferentiated state and the use of deferoxamine, a hypoxia mimetic reagent, has proven to be a suitable strategy to maintain the cells under hypoxic metabolic state. Here, the deferoxamine effects we...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Dietary supplementation with partially hydrolyzed guar gum helps improve constipation and gut dysbiosis symptoms and behavioral irritability in children with autism spectrum disorder.
Authors: Inoue R, Sakaue Y, Kawada Y, Tamaki R, Yasukawa Z, Ozeki M, Ueba S, Sawai C, Nonomura K, Tsukahara T, Naito Y Abstract Prebiotic dietary water-soluble fiber obtained from partially hydrolyzed guar gum was added to diets of children with autism spectrum disorders who presented constipation symptoms. Supplementation with partially hydrolyzed guar gum altered gut microbiota and significantly increased the frequency of defecation per week and altered the gut microbiota. In addition, supplementation with partially hydrolyzed guar gum significantly (p
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Maslinic acid derived from olive fruit in combination with resistance training improves muscle mass and mobility functions in the elderly.
Authors: Nagai N, Yagyu S, Hata A, Nirengi S, Kotani K, Moritani T, Sakane N Abstract Maslinic acid, derived from olive fruit, reduces pro-inflammation cytokines, which are involved in muscle fiber atrophy. Therefore, the maslinic acid ingestion may enhance the muscular response to resistance training through anti-inflammatory action. We therefore conducted a parallel, double-blind, randomized, placebo-controlled trial that examined whether a combination of maslinic acid supplementation and resistance training improve mobility functions in community-dwelling elderly persons. Over a 12-week period, 36 participants u...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Gut microbiota disorders cause type 2 diabetes mellitus and homeostatic disturbances in gut-related metabolism in Japanese subjects.
This study aimed to determine and compare the nutrient intakes and metabolic markers and to elucidate the relationships among these factors in Japanese T2DM patients and control individuals. Fifty-nine Japanese T2DM patients and 59 matched healthy control individuals participated in this study. We examined the differences regarding the participants' dietary habits, microbiota, and fecal short-chain fatty acids, and analyzed the relationships between the gut microbiota and blood metabolic markers in the T2DM patients and the control subjects. The T2DM patients consumed more carbohydrates, and had lower fecal propionate and ...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Possible involvement of thiamine insufficiency in heart failure in the institutionalized elderly.
Authors: Ao M, Yamamoto K, Ohta J, Abe Y, Niki N, Inoue S, Tanaka S, Kuwabara A, Miyawaki T, Tanaka K Abstract Heart failure is a major manifestation of thiamine deficiency; beriberi. Even thiamine insufficiency, milder than deficiency, may be associated with increased heart failure risk. In this cross-sectional study, the relationship between thiamine insufficiency and heart failure was investigated in the Japanese institutionalized elderly from April to November 2017. Fifty-five subjects in four care facilities were evaluated for their whole blood thiamine and plasma brain natriuretic peptide concentrations. Mean...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Association between vitamin B group supplementation with changes in % flow-mediated dilatation and plasma homocysteine levels: a randomized controlled trial.
This study investigated whether low-dose B vitamin supplementation improves endothelial dysfunction in Japanese adults with one or more components of metabolic syndrome. A randomized, controlled, crossover trial, without a washout period or blinding of subjects, was conducted from May-September, 2010. The subjects were 127 Japanese men and women aged 40-65 years who had at least one component of metabolic syndrome without medication. Participants took a supplement drink for two months but were divided into early intervention or later intervention groups. The flow-mediated dilatation, plasma homocysteine level, serum B-vita...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Sex difference: an important issue to consider in epidemiological and clinical studies dealing with serum paraoxonase-1.
Authors: Trentini A, Bellini T, Bonaccorsi G, Cavicchio C, Hanau S, Passaro A, Cervellati C Abstract The aim of this study was to evaluate the influence of sex on serum paraoxonase-1 (PON1) activities and on its relationship with cardiovascular disease risk factors such as overall and central obesity. Arylesterase and lactonase activities of PON1 were assessed in 374 women and 92 men. Both arylesterase and lactonase activities were significantly higher in women compared to men (p
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Correlation of the endoscopic findings for small and large bowels in pediatric patients with established Crohn's disease.
Authors: Okuhira T, Yoden A, Aomatsu T, Akamatsu M, Inoue K, Kaji E, Takitani K, Tamai H Abstract Small bowel capsule endoscopy can detect subtle mucosal lesions in pediatric patients with Crohn's disease, and our aim was to evaluate its application in established ileocolonic Crohn's disease. Colonic inflammation was evaluated with the colonic Simple Endoscopic Score for Crohn's Disease (SES-CD) (excluding the score of the terminal ileum). Small bowel inflammation was evaluated with the Lewis score and/or Capsule Endoscopy Crohn's Disease Activity Index (CECDAI). A Lewis score
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Capabilities of fecal calprotectin and blood biomarkers as surrogate endoscopic markers according to ulcerative colitis disease type.
Authors: Sonoyama H, Kawashima K, Ishihara S, Kotani S, Fukuba N, Oka A, Kusunoki R, Tada Y, Mishima Y, Oshima N, Moriyama I, Yuki T, Onishi K, Kinoshita Y Abstract Fecal calprotectin level in ulcerative colitis patients is correlated with endoscopic findings. However, its association with various ulcerative colitis disease types has not been elucidated. In the present study, we investigated the correlation of fecal calprotectin level with endoscopic findings as compared to blood biomarkers according to ulcerative colitis disease type. Fecal calprotectin as well as the blood biomarkers: C-reactive protein (CRP), wh...
Source: Journal of Clinical Biochemistry and Nutrition - May 31, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Induction of sestrin 2 is associated with fisetin-mediated apoptosis in human head and neck cancer cell lines.
In this study, the influence of fisetin on the growth and apoptosis of HNCCs were examined. In HSC3 cells, fisetin treatment reduced the viability and induced apoptosis. Through the results from the screening of the expression profile of apoptosis-related genes, sestrin 2 (SESN2) was functionally involved in fisetin-mediated apoptosis showing the knockdown of SESN2 by siRNA clearly restored fisetin-induced apoptosis. In addition, fisetin reduced the protein expression levels of phospho-mTOR (p-mTOR) and Mcl-1, which are the downstream molecules of SESN2. It also induced PARP cleavage by inducing an increase in the expressi...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effect of storage and DNA extraction method on 16S rRNA-profiled fecal microbiota in Japanese adults.
Authors: Kawada Y, Naito Y, Andoh A, Ozeki M, Inoue R Abstract The effect of two factors, storage and the bacterial DNA extraction method, that potentially affect the 16S rRNA-based profiling of the microbiota in the feces of Japanese adults, were evaluated. Profiles of the microbiota in feces stored in DESS (DMSO-EDTA-salt solution) for 1, 2 and 3 weeks at room temperature, and for 3 weeks at 4°C were compared with those in fresh feces and feces stored in guanidine thiocyanate solution for 3 weeks at 4°C. None of the storage variables (preservation solution, temperature and duration) considerably affected ...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Human-specific dual regulations of FXR-activation for reduction of fatty liver using in vitro cell culture model.
Authors: Miyazaki T, Honda A, Ikegami T, Iida T, Matsuzaki Y Abstract Nuclear receptor farnesoid X receptor activation inhibits fatty acid synthesis through the liver X receptor-α-sterol regulatory element binding protein-1c pathway universally in animals, but also has human-specific crosstalk with the peroxisome proliferator-activated receptor-α. The effects of farnesoid X receptor-ligands on both the synthesis and degradation of fatty liver through nuclear receptor-related regulation were investigated in both human and murine hepatocytes. A fatty liver culture cell model was established using a synthe...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Uric acid, ferritin and γ-glutamyltransferase can be informative in prediction of the oxidative stress.
Uric acid, ferritin and γ-glutamyltransferase can be informative in prediction of the oxidative stress. J Clin Biochem Nutr. 2019 Mar;64(2):124-128 Authors: Oda K, Kikuchi E, Kuroda E, Yamada C, Okuno C, Urata N, Kishimoto N, Kubo A, Ishii N, Nishizaki Y Abstract The anti-oxidant system is affected not only by aging but also many lifestyle factors. We aimed to clarify the determinants of medical check-up items affecting the anti-oxidant system. We studied 959 Japanese individuals who underwent anti-aging health check-ups (mean age: 61.1 years) at Tokai University from 2006 to 2016. As parameters ...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Probucol induces the generation of lipid peroxidation products in erythrocytes and plasma of male cynomolgus macaques.
Authors: Shichiri M, Ishida N, Hagihara Y, Yoshida Y, Kume A, Suzuki H Abstract We previously reported that probucol, a lipid lowering agent, protected mice from malaria infection via depletion in plasma α-tocopherol. The antioxidant α-tocopherol in host circulation is necessary for the malaria parasites to protect themselves from oxidative stress in erythrocytes where high amounts of reactive oxygen species are generated. To assess the potential for the clinical application of probucol as an anti-malarial therapy, it was necessary to determine the effects of probucol by using primate experiments. Here ...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Heat treatments of ginger root modify but not diminish its antioxidant activity as measured with multiple free radical scavenging (MULTIS) method.
Authors: Sueishi Y, Masamoto H, Kotake Y Abstract Ginger (Zingiber officinale Rosc.) root (or rhizome) has been reported to have antioxidant properties such as reactive oxygen species scavenging activities. Using multiple free-radical scavenging method, we have newly determined the scavenging abilities of ginger roots against five reactive oxygen species, i.e., HO•, O2 -•, RO•, tert-BuOO•, and 1O2. After heating grated ginger roots at 80°C for 2 h, nearly 50% decrease in scavenging ability was recorded against 1O2 and tert-BuOO•. Conversely, the O2 -• scavenging ability increa...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Physiological effects of an oil rich in γ-linolenic acid on hepatic fatty acid oxidation and serum lipid levels in genetically hyperlipidemic mice.
Physiological effects of an oil rich in γ-linolenic acid on hepatic fatty acid oxidation and serum lipid levels in genetically hyperlipidemic mice. J Clin Biochem Nutr. 2019 Mar;64(2):148-157 Authors: Ide T, Origuchi I Abstract We investigated the physiological activity of an oil rich in γ-linolenic acid of evening primrose origin (containing 42.6% γ-linolenic acid) affecting hepatic fatty acid metabolism, and serum lipid levels in genetically hyperlipidemic mice deficient in apolipoprotein E expression. Male apolipoprotein E-deficient mice (BALB/c.KOR/StmSlc-Apoe shl ) were fed exper...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Sudachitin, polymethoxyflavone from Citrus sudachi, enhances antigen-specific cellular and humoral immune responses in BALB/c mice.
We examined the effect of sudachitin on immune response in ovalbumin-immunized BALB/c mice. Treatment with sudachitin increased ovalbumin-specific IL-4 and IL-10 productions. In addition, mice that received sudachitin showed higher levels of ovalbumin-specific IgE, IgG1 and IgG l production than did control mice. The antibody response to the thymus-independent antigen 2,4,6-trinitrophenyl Ficoll was not different in the control and sudachitin groups, suggesting that sudachitin does not directly stimulate antibody production. An in vitro study showed that treatment of sudachitin enhanced the ability of antigen presentation ...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effects of acute footbath before and after glucose ingestion on arterial stiffness.
Authors: Kobayashi R, Hashimoto Y, Okamoto T Abstract The present study investigated the acute effect of a footbath on increases in arterial stiffness after glucose ingestion in healthy young women. Nine healthy young women (aged 18.4 ± 0.2 years; mean ± SE) completed three trials in random order. They started a footbath before 75-g oral glucose ingestion, a footbath after 75-g oral glucose ingestion and no footbath (control) trials. Aortic (carotid-femoral) and leg (femoral-ankle) pulse wave velocity, the carotid augmentation index, carotid, brachial and ankle blood pressure, hear...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Zinc supplementation reduces Candida infections in pediatric intensive care unit: a randomized placebo-controlled clinical trial.
In conclusion, zinc supplementation shows beneficial clinical efficacy in reducing Candida infections among PICU patients on broad-spectrum antibiotics with critical illnesses. PMID: 30936630 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Evaluation of gastric acid suppression with vonoprazan using calcium carbonate breath test.
In conclusion, the amount of gastric acid secretion rapidly decreased by the administration of vonoprazan; this inhibitory effect was found to be potent and long-lasting. (UMIN ID: UMIN000025469). PMID: 30936631 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Influence of gastric endoscopic submucosal dissection on serum opsonic activity measured by chemiluminescence.
This study aimed to elucidate whether changes in serum opsonic activity measured by lucigenin-dependent chemiluminescence and luminol-dependent chemiluminescence are useful for estimating physical stress during the perioperative period of gastric endoscopic submucosal dissection. Serum opsonic activity in the peripheral blood of 87 patients was examined in the morning of the day of endoscopic submucosal dissection, the next day, and at 4 days after endoscopic submucosal dissection. Peak height and area under the curve for lucigenin-dependent chemiluminescence were 106.1 ± 22.7% and 102.0 ± 24.7...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Role of the ISR-ATF4 pathway and its cross talk with Nrf2 in mitochondrial quality control.
Authors: Kasai S, Yamazaki H, Tanji K, Engler MJ, Matsumiya T, Itoh K Abstract Recent investigations have clarified the importance of mitochondria in various age-related degenerative diseases, including late-onset Alzheimer's disease and Parkinson's disease. Although mitochondrial disturbances can be involved in every step of disease progression, several observations have demonstrated that a subtle mitochondrial functional disturbance is observed preceding the actual appearance of pathophysiological alterations and can be the target of early therapeutic intervention. The signals from damaged mitochondria are transf...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Reactivity of redox sensitive paramagnetic nitroxyl contrast agents with reactive oxygen species.
Authors: Nyui M, Nakanishi I, Anzai K, Ozawa T, Matsumoto KI Abstract The reactivity of nitroxyl free radicals, 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL) and 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (CmP), with reactive oxygen species (ROS) were compared as typical 6-membered and 5-membered ring nitroxyl compounds, respectively. The reactivity of the hydroxylamine forms of both these nitroxyl radicals (TEMPOL-H and CmP-H) was also assessed. Two free radical species of ROS, hydroxyl radical (•OH) and superoxide (O2 •-), were subjected to a competing reaction. •OH was generated ...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Direct free radical scavenging effects of water-soluble HMG-CoA reductase inhibitors.
Authors: Umeda R, Takanari H, Ogata K, Matsumoto S, Kitano T, Ono K, Tokumaru O Abstract 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors, statins, are widely used for preventing cardiovascular and cerebrovascular diseases by controlling blood cholesterol level. Additionally, previous studies revealed the scavenging effects of statins on free radicals. We assessed direct scavenging activities of two water-soluble statins, fluvastatin and pravastatin, on multiple free radicals using electron spin resonance spectrometry with spin trapping method. We estimated reaction rate constants (k fv for fluvastatin, a...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Efficacy of high-affinity liposomal astaxanthin on up-regulation of age-related markers induced by oxidative stress in human corneal epithelial cells.
Authors: Shimokawa T, Yoshida M, Fukuta T, Tanaka T, Inagi T, Kogure K Abstract Decreases in tear volume, unstable tear films and excessive tear evaporation are known to cause desiccation and hyperosmolar stress. These, in turn, induce oxidative stress that is thought to cause dry eye, which is also considered to be age-related disease. We hypothesized that oxidative stress induces up-regulation of age-related markers, and that the antioxidant astaxanthin prepared as a liposomal formulation may be a candidate for the treatment of dry eye. Herein, we examined age-related markers in an in vitro dry eye model, and eva...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Proteomic study on neurite responses to oxidative stress: search for differentially expressed proteins in isolated neurites of N1E-115 cells.
Authors: Fukui K, Okihiro S, Ohfuchi Y, Hashimoto M, Kato Y, Yoshida N, Mochizuki K, Tsumoto H, Miura Y Abstract Reactive oxygen species attack several living organs and induce cell death. Previously, we found axonal/dendrite degeneration before the induction of cell death in hydrogen peroxide-treated neuroblastoma: N1E-115 cells and primary neurons. This phenomenon may be connected with membrane oxidation, microtubule destabilization and disruption of intracellular calcium homeostasis. However, its detailed mechanisms are not fully understood. Here, we identified proteins after treatment with hydrogen peroxide usi...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

The preventive effect of Qing Dai on bisphosphonate-induced gastric cellular injuries.
In this study, we investigated whether Qing Dai prevented bisphosphonate-induced gastric cellular injuries. Risedronate (a bisphosphonate) was added to rat gastric mucosal cells. Risedronate-induced cellular injury, cellular lipid peroxidation, mitochondrial membrane potential, and reactive oxygen species production in rat gastric mucosal cells were examined via viable cell counting, specific fluorescent indicators, and electron spin resonance. Pretreatment with Qing Dai attenuated the fluorescence intensity of diphenyl-1-pyrenylphosphine and MitoSox as well as the signal intensities of electron spin resonance. Cell viabil...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Fucoxanthin administration delays occurrence of tumors in xenograft mice by colonospheres, with an anti-tumor predictor of glycine.
Authors: Terasaki M, Matsumoto N, Hashimoto R, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M Abstract Fucoxanthin and its major metabolite, fucoxanthinol, have potent anti-cancer properties in carcinogenic model mice and against cancer cells. Evidence has accumulated regarding the diagnostic potential of biological metabolites as invasive and non-invasive obtainable approaches. We recently demonstrated that glycine was an effective predictor of the suppression of sphere formation and epithelial mesenchymal transition by fucoxanthinol in human colorectal cancer stem-like spheroids (colonospheres) under no...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

5-Aminolevulinic acid with ferrous iron improves early renal damage and hepatic steatosis in high fat diet-induced obese mice.
Authors: Kamiya A, Hara T, Tsuda M, Tsuru E, Kuroda Y, Ota U, Karashima T, Fukuhara H, Inoue K, Ishizuka M, Nakajima M, Tanaka T Abstract 5-Aminolevulinic acid, a natural amino acid, activates mitochondrial respiration and induces heme oxygenase-1 expression. Obesity and type 2 diabetes mellitus are associated with age-related mitochondrial respiration defect, oxidative stress and inflammation. The aim of this study is to investigate the effects of 5-aminolevulinic acid with sodium ferrous citrate on early renal damage and hepatic steatosis. 7-Month-old C57BL/6 mice were fed with a standard diet or high fat diet fo...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Astaxanthin, a xanthophyll carotenoid, prevents development of dextran sulphate sodium-induced murine colitis.
In conclusion, astaxanthin prevented the development of DSS-induced colitis via the direct suppression of NF-κB, AP-1 and MAPK activation. These findings suggest that astaxanthin is a novel candidate as a therapeutic option for the treatment of inflammatory bowel disease. PMID: 30705514 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Positive influence of aqua exercise and burdock extract intake on fitness factors and vascular regulation substances in elderly.
This study investigated the changes due to aqua exercise and burdock extract intake in senior fitness, prostaglandin I2 (PGI2), and thromboxane A2 (TXA2) in elderly women. Forty elderly women (65-80 years) volunteered for this study. After baseline measurements, participants were randomized into control (n = 8), aqua exercise (n = 11), aqua exercise and burdock extract intake combination (n = 11), and burdock extract intake groups (n = 10). The variables of senior fitness tests, PGI2 and TXA2 were measured in all participants before and after the 12-week study. Blood collections were...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Effect of dietary antioxidant-rich foods combined with aerobic training on energy metabolism in healthy young men.
This study aimed to investigate the effect of intake of the specific antioxidant-rich foods coupled with exercise training on energy metabolism. Twenty young healthy, untrained men were assigned to antioxidant and control groups: participants in the antioxidant group were encouraged to consume foods containing catechin, astaxanthin, quercetin, glutathione, and anthocyanin. All participants performed cycle training at 60% maximum oxygen consumption for 30 min, 3 days per week for 4 weeks. Maximum work load was significantly increased by training in both groups, while oxygen consumption during exercise was significantly...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

The comparison of the intensity of human intestinal spirochetes between Brachyspira pilosicoli and Brachyspira aalborgi infections.
Authors: Iwamoto J, Adachi Y, Honda A, Monma T, Matsuzaki Y Abstract The agglutination titers of Brachyspira pilosicoli (B. pilosicoli) and Brachyspira aalborgi (B. aalborgi) were examined in colitis patients with human intestinal spirochetes. Among three cases of colitis patients, the titer of B. pilosicoli was extremely high in two cases while the titer of B. aalborgi was extremely high in one case. These three cases had symptoms of colitis, such as watery diarrhea, and we diagnosed the case as Brachyspira- related colitis. These findings suggest that the agglutination titers of Brachyspira may be useful in cases...
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Eradication therapy for Helicobacter pylori infection improves nutrition status in Japanese hemodialysis patients: a pilot study.
In conclusion, H. pylori eradication may improve nutrition status by increasing serum cholinesterase and cholesterol levels in hemodialysis patients, especially those with mild and moderate gastric mucosal atrophy. PMID: 30705518 [PubMed] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

Erratum.
Authors: Abstract [This corrects the article DOI: 10.3164/jcbn.17-128.]. PMID: 30705519 [PubMed - in process] (Source: Journal of Clinical Biochemistry and Nutrition)
Source: Journal of Clinical Biochemistry and Nutrition - February 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research