CircHIPK3 promotes proliferation and invasion in nasopharyngeal carcinoma by abrogating miR ‐4288‐induced ELF3 inhibition
In conclusion, our findings demonstrated that circHIPK3 facilitated NPC progression through protecting ELF3 from miR‐4288‐mediated silencing, which suggested that the circHIPK3‐miR‐4288‐ELF3 regulatory loop might be a potential target for NPC prev ention. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 20, 2018 Category: Cytology Authors: Zhaoyang Ke,
Fei Xie,
Chaopan Zheng,
Daishi Chen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Brain ‐derived neurotrophic factor promotes proliferation and progesterone synthesis in bovine granulosa cells
We are the first to show stimulatory effects of brain ‐derived neurotrophic factor (BDNF) on proliferation and progesterone (P4) synthesis by bovine granulosa cells (GCs); and that the AKT signaling pathway participates in both proliferation and P4 synthesis induced by BDNF, while the ERK1/2 signaling pathway only participates in cellular proliferation. CCNA1: cyclin A1; CCND1: cyclin D1; CCNE2: cyclin E2; CDK1: cyclin ‐dependent kinase 1; STAR: steroidogenic acute regulatory protein; MAPK: mitogen‐activated protein kinases; PI3K: phosphoinositide 3‐kinase AbstractBrain ‐derived neurotrophic factor (BDNF) is invo...
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Shuxiong Chen,
Fengge Wang,
Zhuo Liu,
Yun Zhao,
Yanwen Jiang,
Lu Chen,
Chunjin Li,
Xu Zhou Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Inhibitory effect of MSH6 gene silencing in combination with cisplatin on cell proliferation of human osteosarcoma cell line MG63
In conclusion, our preliminary results indicated that the combination ofMSH6 gene silencing coupled with DDP may have a better effect on the inhibition of OS cell proliferation and promote apoptosis, potentially providing targets for the OS treatment. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Hu ‐Cheng Liu,
Jin Zeng,
Bin Zhang,
Xu‐Qiang Liu,
Min Dai Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Downregulation of long noncoding RNA LOC101928134 inhibits the synovial hyperplasia and cartilage destruction of osteoarthritis rats through the activation of the Janus kinase/signal transducers and activators of transcription signaling pathway by upregulating IFNA1
This study aims to explore the role of long noncoding RNA (lncRNA) LOC101928134 in the synovial hyperplasia and cartilage destruction, more specifically, in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway in an OA rat model. Microarray ‐based gene expression analysis was conducted to screen out the lncRNA differentially expressed in OA and predict the target gene of the lncRNA with the involvement of the signaling pathway through Kyoto encyclopedia of genes and genomes (KEGG) analysis. A model of OA was established and treated w ith the small interfering RNA LOC101928134/i...
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Da ‐Wei Yang,
Xin Zhang,
Gui‐Bin Qian,
Ming‐Jiu Jiang,
Peng Wang,
Kun‐Zheng Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
New insights into the roles and regulation of SphK2 as a therapeutic target in cancer chemoresistance
Development of multidrug resistance (MDR) is still a big obstacle against successful and complete cancer treatment. Sphingosine kinase 2 targeting could provide a novel approach and a potentially more effective treatment strategy for MDR in cancer. AbstractChemoresistance is a complicated process developed by most cancers and accounts for the majority of relapse and metastasis in cancer. The main mechanisms of chemoresistance phenotype include increased expression and/or activated drug efflux pumps, altered DNA repair, altered metabolism of therapeutics as well as impaired apoptotic signaling pathways. Aberrant sphingolipi...
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Leili Hasanifard,
Roghayeh Sheervalilou,
Maryam Majidinia,
Bahman Yousefi Tags: REVIEW ARTICLE Source Type: research
Inflammatory molecules produced by meniscus and synovium in early and end ‐stage osteoarthritis: a coculture study
Inflammatory mediators, metalloproteinases, and metalloproteinases inhibitors are produced by meniscus and synovium from early and end ‐stage osteoarthritic patients. Production of inflammatory molecules by synovium and meniscus can trigger inflammatory signals in early osteoarthritis (OA) patients, and induce extracellular matrix loss in the progressive and final stages of OA pathology. The interaction between synovium and menis cus is suggested in early and late osteoarthritic patients and it may provide a basis for identifying the mechanisms contributing to the development of OA associated with meniscal tear. Abstract...
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Marta Favero,
Elisa Belluzzi,
Giovanni Trisolino,
Mary B Goldring,
Steven R Goldring,
Augusto Cigolotti,
Assunta Pozzuoli,
Pietro Ruggieri,
Roberta Ramonda,
Brunella Grigolo,
Leonardo Punzi,
Eleonora Olivotto Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
PINK1/Parkin ‐mediated mitophagy promotes apelin‐13‐induced vascular smooth muscle cell proliferation by AMPKα and exacerbates atherosclerotic lesions
In this study, we aimed to investigate the role of mitophagy in apelin‐13‐induced VSMC proliferation and atherosclerotic lesions in apolipoprotein E knockout (ApoE‐/‐) mice. Apelin‐13 enh ances human aortic VSMC proliferation and proliferative regulator proliferating cell nuclear antigen expression in dose and time‐dependent manner, while is abolished by APJ antagonist F13A. We observe the engulfment of damage mitochondria by autophagosomes (mitophagy) of human aortic VSMC in apeli n‐13 stimulation. Mechanistically, apelin‐13 increases p‐AMPKα and promotes mitophagic activity such as the LC3I to LC3II ra...
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Lu He,
Qionglin Zhou,
Zheng Huang,
Jin Xu,
Hong Zhou,
Deguan Lv,
Liqun Lu,
Shifang Huang,
Mingzhu Tang,
Jiuchang Zhong,
Jianxiong Chen,
Xulin Luo,
Lanfang Li,
Linxi Chen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Annexin A3 gene silencing promotes myocardial cell repair through activation of the PI3K/Akt signaling pathway in rats with acute myocardial infarction
In this study, we examine the inhibition of annexin A3 (ANXA3) on AMI through the phosphatidylinositide 3 ‐kinase/protein kinase B (PI3K/Akt) signaling pathway. We selected rats to build an AMI model which was then assigned into different groups. The hemodynamic parameters after transfection were detected by using enzyme‐linked immunosorbent assay. The effect of silencing of ANXA3 on inflammatory re action and the PI3K/Akt signaling pathway was assessed. Rats transfected with ANXA3‐short hairpin RNA had alleviated hemodynamics, inflammatory reaction, decreased infarct size, α‐smooth muscle actin, Collagen I, and C...
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Hua Meng,
Yan Zhang,
Song ‐Tao An,
Yan Chen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Glutamate triggers intracellular Ca2+ oscillations and nitric oxide release by inducing NAADP ‐ and InsP3‐dependent Ca2+ release in mouse brain endothelial cells
Glutamate induces intracellular Ca2+ oscillations in mouse brain microvascular endothelial cells. Glutamate ‐induced intracellular Ca2+ oscillations lead to robust, although delayed, nitric oxide release. Brain microvascular endothelial cells could play a crucial role in neurovascular coupling during synaptic activity. The neurotransmitter glutamate increases cerebral blood flow by activating postsynaptic neurons and presynaptic glial cells within the neurovascular unit. Glutamate does so by causing an increase in intracellular Ca2+ concentration ([Ca2+]i) in the target cells, which activates the Ca2+/Calmodulin ‐dep...
Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Estella Zuccolo,
Dlzar A. Kheder,
Dmitry Lim,
Angelica Perna,
Francesca Di Nezza,
Laura Botta,
Giorgia Scarpellino,
Sharon Negri,
Simona Martinotti,
Teresa Soda,
Greta Forcaia,
Laura Riboni,
Elia Ranzato,
Giulio Sancini,
Luigi Ambrosone,
Eg Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Prostacyclin: A major prostaglandin in the regulation of adipose tissue development
Prostacyclin is a major prostaglandin of the arachidonate cyclooxygenase (COX) pathway. It mediates its action through a prostacyclin receptor (IP), triggers the terminal differentiation of adipocytes, and can work in both autocrine and paracrine manners. It can also induce the transformation of white adipose tissues to form thermogenic brite (brown ‐like) adipose tissues, indicating its potential to treat obesity. Prostaglandins (PGs) belong to the group lipid mediators and can act as local hormones. They contain 20 carbon atoms, including a 5 ‐carbon ring, and are biosynthesized from membrane phospholipid derived ara...
Source: Journal of Cellular Physiology - November 15, 2018 Category: Cytology Authors: Mohammad Sharifur Rahman Tags: REVIEW ARTICLE Source Type: research
Agomelatine modulates calcium signaling through protein kinase C and phospholipase C ‐mediated mechanisms in rat sensory neurons
The obtained results of agomelatine ‐induced [Ca2+]i signals indicates that peripheral mechanisms are involved in analgesic effects of agomelatine. Agomelatine seems to possess antinociceptive properties which may be of clinical value AbstractAgomelatine, a novel antidepressant exerting its effects through melatonergic and serotonergic systems, implicated to be effective against pain including neuropathic pain but without any knowledge of mechanism of action. To explore the possible role of agomelatine on nociceptive transmission at the peripheral level, the effects of agomelatine on intracellular calcium ([Ca2+]i) signa...
Source: Journal of Cellular Physiology - November 15, 2018 Category: Cytology Authors: Ihsan Serhatlioglu,
Batuhan Bilgin,
Emine Kacar,
Sibel Ozcan,
Sinan Canpolat,
Ahmet Ayar,
Haluk Kelestimur,
Mete Ozcan Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
MicroRNA ‐99a‐5p suppresses breast cancer progression and cell‐cycle pathway through downregulating CDC25A
In this study, we aimed to explore the association between miR ‐99a‐5p andCDC25A in breast cancer and the regulatory mechanisms of miR ‐99a‐5p on breast cancer. The expressions of messenger RNA and microRNAs in breast cancer tissues and adjacent tissues were analyzed by the Cancer Genome Atlas microarray analysis. Quantitative real‐time polymerase chain reaction was conducted to find out the expression levels of miR‐99a‐ 5p andCDC25A. The expression levels of proteins (CDC25A, ki67, cyclin D1, p21, BAX, BCL ‐2, BCL‐XL, MMP2, and MMP9) were determined by Western blot analysis. The relationship between miR...
Source: Journal of Cellular Physiology - November 15, 2018 Category: Cytology Authors: Hongzhen Qin,
Wenfei Liu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
A study of quiet eye's phenomenon in the shooting section of “laser run” of modern pentathlon
The study is the first evaluating visual behavior in the “Laser Run” section of modern pentathlon in elite athletes. The results provide insightful information about different cognitive and perceptual processes involved in Modern Pentathlon's athletes' performances at both elite and non‐elite level. AbstractThe aim of the study was to evaluate the effects of the Quiet eye (QE) phenomenon on performances during the shooting section of “Laser Run” of Modern Pentathlon, in two samples of athletes (novices and experts). The “Laser Run” consists of running and shooting activities. The study involved 18 experienced...
Source: Journal of Cellular Physiology - November 15, 2018 Category: Cytology Authors: Andrea Chirico,
Dario Fegatelli,
Federica Galli,
Luca Mallia,
Fabio Alivernini,
Susanna Cordone,
Francesco Giancamilli,
Stefano Pecci,
Gian Marco Tosi,
Antonio Giordano,
Fabio Lucidi,
Mina Massaro Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Circular RNA CCDC66 targets DCX to regulate cell proliferation and migration by sponging miR ‐488‐3p in Hirschsprung's disease
In conclusion, this is the first report revealing that cir‐CCDC66 modulates DCX expression through sponging miR‐488‐3p and thus participates in the onset of HSCR. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 15, 2018 Category: Cytology Authors: Zechao Wen,
Qiyang Shen,
Hua Zhang,
Yang Su,
Zhongxian Zhu,
Guanglin Chen,
Lei Peng,
Hongxing Li,
Chunxia Du,
Hua Xie,
Xiaoqun Xu,
Weibing Tang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Organ ‐on‐chip models: Implications in drug discovery and clinical applications
In this study, we review the recent advances in in vitro modeling including three‐dimensi onal (3D) organoids, 3D microfabrication, and 3D bioprinting for various organs including the heart, kidney, lung, gastrointestinal tract (intestine–gut–stomach), liver, placenta, adipose, retina, bone, and brain as well as multiorgan models. The availability of organ‐on‐chip models provides a wealth of opportunities to understand the pathogenesis of human diseases and provide a potentially better model to screen a drug, as these models utilize a dynamic 3D environment similar to the human body. Although there are limitation...
Source: Journal of Cellular Physiology - November 15, 2018 Category: Cytology Authors: Rahul Mittal,
Frank W. Woo,
Carlo S. Castro,
Madeline A. Cohen,
Joana Karanxha,
Jeenu Mittal,
Tanya Chhibber,
Vasanti M. Jhaveri Tags: REVIEW ARTICLE Source Type: research
