Extended proliferation of chicken ‐ and Okinawa rail‐derived fibroblasts by expression of cell cycle regulators
AbstractAlthough immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian ‐derived cells. We introduced the simian virus 40 T antigen (SV40T) and human papillomavirus(HPV) ‐E6E7 to chick and Okinawa rail (endangered species)derived fibroblast. As a result, neither theSV40T norE6E7 genes could induce avian cell immortality. Accordingly, we attempted to use a recently developed immortalization method, which involved the coexpression of mutant cyclin ‐dependent kinase 4 (CDK4), Cyclin ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Masafumi Katayama, Tohru Kiyono, Hitomi Ohmaki, Takahiro Eitsuka, Daiji Endoh, Miho Inoue ‐Murayama, Nobuyoshi Nakajima, Manabu Onuma, Tomokazu Fukuda Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A review of the anti ‐inflammatory properties of antidiabetic agents providing protective effects against vascular complications in diabetes
AbstractThe global prevalence of Type 2 diabetes mellitus and its associated complications are growing rapidly. Although the role of hyperglycemia is well recognized in the pathophysiology of diabetic complications, its exact underlying mechanisms are not fully understood. In this regard, accumulating evidence suggests that the role of inflammation appears pivotal, with studies showing that most diabetic complications are associated with an inflammatory response. Several classes of antidiabetic agents have been introduced for controlling glycemia, with evidence that these pharmacological agents may have modulatory effects ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Habib Yaribeygi, Stephen L. Atkin, Matteo Pirro, Amirhossein Sahebkar Tags: REVIEW ARTICLE Source Type: research

Interleukin ‐18 and diabetic nephropathy: A review
AbstractThe inflammatory response has an important role in the pathophysiology of diabetic nephropathy that is contributed to by inflammatory mediators such as interleukin ‐1 (IL‐1), IL‐6, IL‐18, tumor necrosis factor‐α, and macrophage chemotactic protein‐1; however, the role of IL‐18 seems to be more specific than other cytokines in the inflammatory process. IL‐18 is expressed in renal tissue and is upregulated by several stimuli including hyperglyce mia. The expression/urinary level of IL‐18 is positively correlated with the progression of diabetic nephropathy and the urinary albumin excretion rat...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Habib Yaribeygi, Stephen L. Atkin, Amirhossein Sahebkar Tags: REVIEW ARTICLE Source Type: research

Inactivation of ADAMTS18 by aberrant promoter hypermethylation contribute to lung cancer progression
Our results demonstrated that the tumor suppressor gene ADAMTS18 was downregulated in lung cancer by promoter CpG methylation, and it promoted sensitivity to cisplatin via epidermal growth factor receptor/protein kinase B signaling. Our study suggests that ADAMTS18 promoter methylation is a potential epigenetic biomarker for early detection of lung cancer and warrants investigation as therapeutic target for early ‐stage lung cancer. AbstractLung cancer is the most frequently diagnosed cancer worldwide. Epigenetic regulation contributes to lung cancer pathogenesis. TheADAMTS18 tumor suppressor gene is inactivated in some ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Yan Zhang, Hongying Xu, Junhao Mu, Shuliang Guo, Lin Ye, Dairong Li, Weiyan Peng, Xiaoqian He, Tingxiu Xiang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Noncoding transcribed ultraconserved region (T ‐UCR) UC.48+ is a novel regulator of high‐fat diet induced myocardial ischemia/reperfusion injury
High ‐fat diet (HFD) is an independent risk factor for myocardial ischemia/reperfusion (MI/R) injury. Uc.48+ is a long noncoding RNA (lncRNA) from a transcribed ultraconserved region (T‐UCR) of human, mouse, and rat genomes. Here, we explored the aggravating role of uc.48+ and identified purinerg ic P2X7 receptor (P2X7R) as a downstream regulator of uc.48+ in HFD‐induced MI/R vulnerability. Targeting uc.48+ may be a novel therapeutic approach of MI/R vulnerability to HFD. AbstractIncreasing evidence has suggested high ‐fat diet (HFD) is an independent risk factor for myocardial ischemia/...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Lu Ding, Chengxin Gong, Jiani Zhao, Xingzi Liu, Tao Li, Shenqiang Rao, Shuo Wang, Yuanyuan Liu, Shanping Peng, Wen Xiao, Chaopeng Xiong, Rumeng Wang, Shangdong Liang, Hong Xu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Understanding molecular biology of codon usage in mitochondrial complex IV genes of electron transport system: Relevance to mitochondrial diseases
The mitochondrial cytochrome oxidase (CO) genes are involved in complex IV of the electron transport system (ETS), and dysfunction of CO genes leads to several diseases.  nHowever, no work has been reported on the codon usage pattern of these genes. We used bioinformatic methods to analyze the compositional properties and the codon usage pattern of the COI, COII, and COIII genes in fishes, birds, and mammals to understand the similarities and dissimilarities of cod on usage in these genes, which gave an insight into the molecular biology of these genes. AbstractThe mitochondrial cytochrome oxidase (CO) genes are invol...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Arif Uddin, Tarikul Huda Mazumder, Supriyo Chakraborty Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

CircRNA circPDSS1 promotes the gastric cancer progression by sponging miR ‐186‐5p and modulating NEK2
This study mainly researched the effects of circPDSS1 and its related genes in gastric cancer (GC). 2.It revealed part of a complex regulation system, circPDSS1/miR ‐186‐5p/NEK2 axis in GC, and provided a potential therapeutic target for GC treating. 3.Further research should be done to investigate the related genes in this regulation system. AbstractThe aim of this study is to investigate the regulatory mechanism of circPDSS1/miR ‐186‐5p/NEK2 axis on the viability and proliferation in gastric cancer (GC) cell line. Differentially expressed circRNAs, miRNAs, and mRNAs in GC tissues and paracarcinoma tissues were ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Yiming Ouyang, Yuejin Li, Yingguang Huang, Xing Li, Yu Zhu, Yaxin Long, Yongzhi Wang, Xiaodong Guo, Kunmei Gong Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The role of DEAD ‐box RNA helicase p68 (DDX5) in the development and treatment of breast cancer
Discussion of the precise role of p68 in cancer is complex and depends on the cellular microenvironment and interacting factors. In terms of the deregulated expression of p68 in breast cancer and the high prevalence of this cancer among women, it can be informative to review the precise function of this factor in the breast cancer. Therefore, an attempt will be made in this review to clarify the tumorigenic function of p68 in association with its targeting potential for the treatment of breast cancer. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Vida Hashemi, Ali Masjedi, Bita Hazhir ‐karzar, Asghar Tanomand, Siamak Sandoghchian Shotorbani, Mohammad Hojjat‐Farsangi, Ghasem Ghalamfarsa, Gholamreza Azizi, Enayat Anvari, Behzad Baradaran, Farhad Jadidi‐Niaragh Tags: REVIEW ARTICLE Source Type: research

Mitochondrial quality control in cardiac cells: Mechanisms and role in cardiac cell injury and disease
Parkin ‐mediated mitophagy in Drosophila heart. (a) Parkin deficiency in Drosophila heart impaired mitophagy and led to the development of dilated cardiomyopathy. (b) Simultaneous deletion of mitochondrial assembly regulatory factor and Parkin rescued cardiomyopathy (Bhandari et al.,#jcp27597-bib-0005).#jcp27597-bib-0005 AbstractMitochondria play an important role in maintaining cardiac homeostasis by supplying the major energy required for cardiac excitation –contraction coupling as well as controlling the key intracellular survival and death pathways. Healthy mitochondria generate ATP molecules through an aerobic...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Farzaneh G. Tahrir, Dianne Langford, Shohreh Amini, Taha Mohseni Ahooyi, Kamel Khalili Tags: REVIEW ARTICLE Source Type: research

Estrogen deprivation aggravates intracellular calcium dyshomeostasis in the heart of obese ‐insulin resistant rats
Estrogen deprivation had a strong impact on abnormal cardiac function through intracellular calcium regulation. Our findings showed that in obese ‐insulin resistant rats, estrogen deprivation severely aggravates cardiac dysfunction via increased impairment of calcium homeostasis. AbstractThe incidence of cardiovascular disease and metabolic syndrome increases after the onset of menopause, giving evidence for the vital role of estrogen. Intracellular calcium [Ca2+]i regulation plays an important role in the maintenance of left ventricular (LV) contractile function. Although either estrogen deprivation or obesity has been ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Siripong Palee, Wanitchaya Minta, Duangkamol Mantor, Wissuta Sutham, Sasiwan Kerdphoo, Wasana Pratchayasakul, Siriporn C. Chattipakorn, Nipon Chattipakorn Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A2a adenosine receptor agonist improves endoplasmic reticulum stress in MIN6 cell line through protein kinase A/ protein kinase B/ Cyclic adenosine monophosphate response element ‐binding protein/ and Growth Arrest And DNA‐Damage‐Inducible 34/ eukaryotic Initiation Factor 2α pathways
A2a adenosine receptor activation improved endoplasmic reticulum (ER) stress in pancreatic beta cells (PBCs) and its signaling may provide new approach to control ER stress in the PBC cells. AbstractEndoplasmic reticulum (ER) stress is one of the main molecular events underlying pancreatic beta cell (PBC) failure, apoptosis, and a decrease in insulin secretion. Recent studies have highlighted the fundamental role of A2a adenosine receptor (A2aR) in potentiation of insulin secretion and proliferation of PBCs. However, possible protective effects of A2aR signaling against ER stress have not been elucidated yet. Thus, in the ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Fatemeh P. Arasi, Mohammad K. Shahrestanaki, Mahmoud Aghaei Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

MiRNAs and inflammatory bowel disease: An interesting new story
MicroRNAs (miRNAs) are a set of noncoding RNA molecules that are involved in the regulation of the gene expression. We discussed the critical roles of some miRNAs in the pathophysiology, diagnosis, and treatment of IBD. AbstractInflammatory bowel disease (IBD), as a chronic and recurrent inflammatory disorder, is caused by a dysregulated and aberrant immune response to exposed environmental factors in genetically susceptible individuals. Despite huge efforts in determining the molecular pathogenesis of IBD, an increasing worldwide incidence of IBD has been reported. MicroRNAs (miRNAs) are a set of noncoding RNA molecules t...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Soheila Moein, Mostafa Vaghari ‐Tabari, Durdi Qujeq, Maryam Majidinia, Seyed Mohammad Nabavi, Bahman Yousefi Tags: REVIEW ARTICLE Source Type: research

Effects of microRNA ‐217 on proliferation, apoptosis, and autophagy of hepatocytes in rat models of CCL4‐induced liver injury by targeting NAT2
This study aims to explore the effects of miR ‐217 targeting NAT2 on hepatocyte proliferation, apoptosis, and autophagy following carbon tetrachloride (CCL4)‐induced liver injury. Rat models of CCL4‐induced liver injury were established. Healthy Wistar rats were randomized into the normal, blank, negative control (NC), microRNA‐217 (miR ‐217) mimic, miR‐217 inhibitor, small interfering RNA (siRNA)‐N‐acetyltransferase 2 (NAT2), and miR‐217 inhibitor + siRNA‐NAT2 groups. NAT2 activity was evaluated with reversed‐phase high‐performance liquid chromatographic method. Immunohistochemis...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Cheng ‐Liang Yang, Xiao‐Li Zheng, Ke Ye, Ya‐Nan Sun, Yu‐Fei Lu, Hong Ge, Hui Liu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Inflammation and pericarditis: Are neutrophils actors behind the scenes?
The morbidity of acute pericarditis is increasing overtime. Apart the known impairment of the adaptive immunity, recently a large body evidence indicated the central role of the innate immune system in the pathogenesis of recurrent pericarditis, starting from similarities with autoinflammatory diseases, with the “inflammasome” playing an important role. The role of the adaptive immune system in pericarditis cannot be reduced to a black or white issue as these mechanisms often overlap and new therapeutic strategies should be investigated in this direction. AbstractThe morbidity of acute pericarditis is increasin...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Aldo Bonaventura, Fabrizio Montecucco Tags: MINI ‐REVIEW Source Type: research

Exosomes derived from acute myeloid leukemia cells promote chemoresistance by enhancing glycolysis ‐mediated vascular remodeling
AbstractAcute myeloid leukemia (AML) is the most common type of leukemia in adults. AML cells secrete angiogenic factors to remodel vasculature and acquire chemoresistance; however, antiangiogenic drugs are often ineffective in AML treatment. Cancer cell ‐derived exosomes can induce angiogenesis, but their role in vascular remodeling during AML is unclear. Here, we found that exosomes secreted by AML cells promoted proliferation and migration and tube‐forming activity of human umbilical vein endothelial cells (HUVECs), whereas HUVECs conferred c hemoresistance to AML cells. AML cell‐derived exosomes contained vascula...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Bin Wang, Xiaoting Wang, Diyu Hou, Qian Huang, Weiwu Zhan, Canwei Chen, Jingru Liu, Ruolan You, Jieqiong Xie, Ping Chen, Huifang Huang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Four long noncoding RNAs as potential prognostic biomarkers for hepatocellular carcinoma
In this study, we identified 212 differentially expressed lncRNAs and 7,577 differentially expressed genes between liver HCC tumor tissues and normal tissue samples. And then, combining with clinical information, a total of 11 lncRNAs and 162 genes as HCC biomarkers were identified by comprehensive bioinformatics analysis. Further, through coexpress network analysis, we confirmed four lncRNAs (lncRNA_ANKRD10.IT1, lncRNA_CTD.2583A14.8, lncRNA_RP11.404P21.3, and lncRNA_RP11.488L18.10) that can serve as prognostic biomarkers for HCC. The four lncRNAs identified in this study may serve as a potential therapy target for HCC. Ab...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Jianchu Wang, Jian Pu, Tianwei Yao, Xiaojie Lu, Yibin Deng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

MicroRNA ‐382 silencing induces a mitonuclear protein imbalance and activates the mitochondrial unfolded protein response in muscle cells
In the current study, microRNA ‐382‐5p was identified as a regulator of mitochondrial biogenesis and dynamics at mRNA levels. Silencing of miRNA‐382‐5p in C2C12 myotubes revealed a collective downregulation of mitochondrial ribosomal proteins and respiratory chain proteins. MiR‐382 silenced C2C12 showed a mitonuclear pr otein imbalance that led to the activation of the mitochondrial unfolded protein response and a reduced basal oxygen consumption rate without affecting mitochondrial content. AbstractProper mitochondrial function plays a central role in cellular metabolism. Various diseases as well as aging are as...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Dennis Dahlmans, Alexandre Houzelle, P énélope Andreux, Xu Wang, Johanna A. Jörgensen, Norman Moullan, Sabine Daemen, Sander Kersten, Johan Auwerx, Joris Hoeks Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Between an ugly truth and a perfect lie: Wiping off fearful memories using beta ‐adrenergic receptors antagonists
This review illustrates the role of propranolol in fear memory with demonstration of studies that support or oppose its beneficial effect. AbstractPsychiatric disorders such as anxiety, phobias, and post ‐traumatic stress disorder are considered of high global prevalence. Currently, a therapeutic approach to treat these disorders using beta‐blockers, which antagonize the beta‐adrenergic receptors (B1, B2, and B3) is being studied. This approach claims that beta‐blockers, such as propranolol, inhibit fear memory reconsolidation. However, there are several studies refuting such claims by discrediting their experiment...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Abdelrahman M. AlOkda, Mostafa M. Nasr, Shaimaa N. Amin Tags: REVIEW ARTICLE Source Type: research

miR ‐124 promotes neural differentiation in mouse bulge stem cells by repressing Ptbp1 and Sox9
miR ‐124 andlet ‐7b are upregulated in hair follicle bulge cells after neural differentiation.Upregulation ofmiR ‐124 triggers neural differentiation.Ptbp1 andSox9 mRNAs are directmiR ‐124 targets during neural differentiation of hair follicle stem cells (HFSCs). AbstractHair follicle stem cells (HFSCs) are able to differentiate into neurons and glial cells. Distinct microRNAs (miRNAs) regulate the proliferation and differentiation of HFSCs. However, the exact role of miR ‐124 in the neural differentiation of HFSCs has not been elucidated. HFSCs were isolated from mouse whisker follicles.miR ‐9,let ‐7b, andmi...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Haleh Mokabber, Nowruz Najafzadeh, Mohammad Mohammadzadeh Vardin Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

TMEM206 promotes the malignancy of colorectal cancer cells by interacting with AKT and extracellular signal ‐regulated kinase signaling pathways
In this study, we examined the roles of TMEM206 in CRC.MethodReal ‐time reverse transcription polymerase chain reaction was used to measureTMEM206 messenger RNA (mRNA) levels in clinical specimens and transfected cell lines. Immunohistochemistry was used to determine the relationship between TMEM206 expression levels and clinical data. Plasmids and small interfering RNA were used to upregulate and silence TMEM206, respectively. Protein expression levels and signaling pathway modulation were validated through western blot analysis. Colony formation, MTT, cell migration and invasion assays, and flow cytometry analyses were...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Jinbo Zhao, Dehua Zhu, Xiupeng Zhang, Yong Zhang, Jianping Zhou, Ming Dong Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150glued
In this study, we found that cell proliferation was reduced by CQ treatment in time and dose ‐dependent manners. No obvious cell death was detected; however, what was observed instead was G0/G1 arrest during which primary cilium started to grow in the presence of CQ. Pharmacological inhibition of primary cilium formation led to a reduction of cell viability suggesting that CQ‐induced pr imary cilium protected cells from death. In addition to cell growth, with the CQ treatment the retina pigmented epithelium (RPE) cells less flattened with the spindle‐like protrusion. When checking the microtubule networks, the microt...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Ting ‐Yu Chen, Wei‐Chih Lien, Hui‐Ling Cheng, Ta‐Shen Kuan, Shi‐Yuan Sheu, Chia‐Yih Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

MicroRNAs and signaling networks involved in epithelial –mesenchymal transition
In nature, MT participates in many physiological and pathological processes including embryonic development, organogenesis, wound healing, and cancer metastasis. Many aspects regulate EMT including: transcription factors, gene expression patterns, and signaling pathway crosstalk. MicroRNAs as noncoding genes play a distinct role in the progression or suppression of EMT. AbstractEpithelial –mesenchymal transition (EMT) is a phenomenon in which epithelial cells lose their cell‐to‐cell connection and are detached from the base membrane. EMT is fundamental for many biological processes such as embryonic development a...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Mohammad Hossein Musavi Shenas, Shadi Eghbal ‐Fard, Vahid Mehrisofiani, Nima Abd Yazdani, Omid Rahbar Farzam, Faroogh Marofi, Mehdi Yousefi Tags: REVIEW ARTICLE Source Type: research

Licochalcone A attenuates abdominal aortic aneurysm induced by angiotensin II via regulating the miR ‐181b/SIRT1/HO‐1 signaling
This study aimed to investigate the role and underlying mechanism of LA in the abdominal aortic aneurysm (AAA). AAA model was established by continuous infusion of 1000 ng/kg/min of angiotensin II (AngII) in ApoE‐/‐ mice for 4 weeks. At 7 days before AngII administration, 5  mg/kg/day or 10 mg/kg/day of LA was intraperitoneally administered to mice and continued for 4 weeks. The characteristics and quantification of AAAs were determined in situ. Real‐time PCR or western blot was used to measure mRNA or protein levels of matrix metalloproteinase 2 and matrix metal loproteinase 9; pro‐inflammator...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Xuhui Hou, Songbai Yang, Yan Zheng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Long ‐term evaluation of corneal sub‐basal nerve recovery after photorefractive keratectomy and influence of pars plana vitrectomy
The aim of the study is to evaluate corneal sub ‐basal nerves (SBN) a mean of 15.6 years after photorefractive keratectomy (PRK) and the effects on them of pars plana vitrectomy (PPV), using in vivo confocal microscopy (IVCM). PRK eyes showed reduced SBN plexus compared with non‐PRK eyes. In addition, SBN density showed a slow but continuous recovery process up to the end of our follow‐up (25 years) after PRK. Both PRK and non‐PRK eyes showed a marked reduction in SBN density 6 months after PPV. AbstractThe corneal sub ‐basal nerve (SBN) plexus is destroyed during photorefractive keratectomy (PRK) and its recover...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Gian Marco Tosi, Gianluca Martone, Tommaso Bacci, Antonio Tarantello, Stefano Baiocchi, Davide Marigliani, Gabriele Cevenini, Flavia Menicacci, Gianni Virgili, Giacomina Massaro ‐Giordano Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A comparison of three strategies for biopanning of phage ‐scFv library against diphtheria toxin
In conclusion, the results of our study indicate that soluble antibody‐capturing method is an efficient method for isolation of specific scFv fragments. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Mostafa Lakzaei, Mohhamad Javad Rasaee, Ali Akbar Fazaeli, Mahdi Aminian Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Troxerutin attenuates myocardial cell apoptosis following myocardial ischemia ‐reperfusion injury through inhibition of miR‐146a‐5p expression
In conclusion, troxerutin exerted cardioprotective effects during I/R injury by downregulating miR‐146a‐5p. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Liliang Shu, Wanzhe Zhang, Gongcheng Huang, Chen Huang, Xiaohua Zhu, Gang Su, Jing Xu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Formation and activation induction of primordial follicles using granulosa and cumulus cells conditioned media
Follicular activation with granulosa conditioned medium. AbstractFertility preservation of prepubertal girls subjected to invasive cancer therapy necessitates defining protocols for activation of isolated primordial follicles. Granulosa (GCs) and cumulus cells (CCs) play pivotal role in oocyte development. Although GCs and CCs share some similarities, they differ in growth factors production. The current study was conducted to evaluate the effects of GCs, CCs and their conditioned media on mice primordial follicles activation. One ‐day‐old mice ovaries were subjected to 6‐day culture with base medium (BM), GC conditi...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Mohammad Jafari Atrabi, Vahid Akbarinejad, Ramazan Khanbabaee, Azam Dalman, Christiani Andrade Amorim, Mustafa Najar ‐Asl, Mojtaba Rezazadeh Valojerdi, Rouhollah Fathi Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The effect of Activin ‐A on periodontal ligament fibroblasts‐mediated osteoclast formation in healthy donors and in patients with fibrodysplasia ossificans progressiva
This study brings new insight on the role of Activin A in osteoclast formation, which may further add to understanding FOP pathophysiology; in addition to the known Activin ‐A‐mediated HO, this study shows that Activin‐A may also inhibit osteoclast formation, thereby further promoting HO formation. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Ton Schoenmaker, Fenne Wouters, Dimitra Micha, Tim Forouzanfar, Coen Netelenbos, E. Marelise W. Eekhoff, Nathalie Bravenboer, Teun J. de Vries Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Cancer stem cells (CSCs) in cancer progression and therapy
Most of the tumors are occupied with a number of self ‐renewing cells called cancer stem cells (CSCs) that are contributed to the initiation, maintenance, and thriving cancer. The cells have rather similar characteristics to other stem cells located in the niche of body organs, but they have not essentially the same responses to the diverse stimuli. There is evidence for repopulation of CSCs after treatment with chemo/radiotherapy, which is possibly because of their highly plastic feature. Normal stem cells have the proclivity to transform into CSCs when they undergo continuous mutagenesis or receive tumorigenic signals ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Masoud Najafi, Bagher Farhood, Keywan Mortezaee Tags: REVIEW ARTICLE Source Type: research

Clonal hematopoiesis: Genes and underlying mechanisms in cardiovascular disease development
We review the genes and mechanisms of clonal hematopoiesis of indeterminate potential (CHIP) and cancer and cardiovascular disease (CVD) development. We discuss targeting mutated genes, receptors and growth factors in hematopoietic stem cells regulation and CHIP to control CVD disease. Also, we suggest novel strategies for treatment of cells and pathways involved in regulation of HSCs can be used to manage CVD treatment AbstractThe clonal hematopoiesis when occurring without hematologic abnormalities is defined as clonal hematopoiesis of indeterminate potential (CHIP). Aging causes accumulation of somatic mutations, and he...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Habib Haybar, Saeid Shahrabi, Majid Ghanavat, Elahe Khodadi Tags: REVIEW ARTICLE Source Type: research

Mitochondrial dysfunction in diabetes and the regulatory roles of antidiabetic agents on the mitochondrial function
The prevalence of type 2 diabetes mellitus (T2DM) is increasing rapidly with its associated morbidity and mortality; many pathophysiological pathways such as oxidative stress, inflammatory responses, adipokines, obesity ‐induced insulin resistance, improper insulin signaling, and beta cell apoptosis are associated with the development of T2DM. There is increasing evidence of the role of mitochondrial dysfunction in the onset of T2DM, particularly in relation to the development of diabetic complications. Here, the role of mitochondrial dysfunction in T2DM is reviewed together with its modulation by antidiabetic therapeuti...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Habib Yaribeygi, Stephen L. Atkin, Amirhossein Sahebkar Tags: REVIEW ARTICLE Source Type: research

Calpain ‐2 protects against heat stress‐induced cardiomyocyte apoptosis and heart dysfunction by blocking p38 mitogen‐activated protein kinase activation
In conclusion, our study suggests that heat stress reduces calpain‐2 expression, which then activates p38, leading to cardiomyocyte apoptosis and heart dysfunct ion. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Zhi ‐feng Liu, Jing‐jing Ji, Dong Zheng, Lei Su, Tianqing Peng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Sulfonyl(thio)urea derivative induction of insulin secretion is mediated by potassium, calcium, and sodium channel signal transduction
ConclusionsSD diminish glycemia by inducing GLP ‐1 secretion and inhibiting disaccharidases. To our knowledge, this is the first report of an insulin secretagogue effect of SD that is mediated by potassium and calcium, as well as sodium, signal transduction. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Paola Miranda Sulis, Betina Fernanda Dambr ós, Alessandra Mascarello, Adair Roberto Soares dos Santos, Rosendo Augusto Yunes, Ricardo José Nunes, Marisa Jádna Silva Frederico, Fátima Regina Mena Barreto Silva Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Ecto ‐5′‐nucleotidase (CD73) is a potential target of hepatocellular carcinoma
CD73 promotes hepatocellular carcinoma (HCC) growth and metastasis, in vitro and in vivo, mediated by epithelial growth factor receptor (EGFR). AbstractHigh expression of ecto ‐5′‐nucleotidase (CD73) has been reported in a number of epithelium origin malignancies. Here, we hypothesize that CD73 promotes hepatocellular carcinoma (HCC) growth and metastasis and that the effect is mediated by epithelial growth factor receptor (EGFR). HCC cells with different malignanci es and Tissue microarrays of the tumor and peritumoral liver tissues from 30 independent patients were used to examine CD73 and EGFR expression. Then...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Shalaimaiti Shali, Jiangang Yu, Xin Zhang, Xue Wang, Youping Jin, Minsheng Su, Xiaohong Liao, Jerry Yu, Xiuling Zhi, Ping Zhou Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Fifteen hub genes associated with progression and prognosis of clear cell renal cell carcinoma identified by coexpression analysis
In this study, our aim is to identify potential biomarkers that could effectively predict the prognosis and progression of ccRCC. First, we used The Cancer Genome Atlas (TCGA) RNA ‐sequencing (RNA‐seq) data of ccRCC to identify 2370 differentially expressed genes (DEGs). Second, the DEGs were used to construct a coexpression network by weighted gene coexpression network analysis (WGCNA). Moreover, we identified the yellow module, which was strongly related to the histolog ic grade and pathological stage of ccRCC. Then, the functional annotation of the yellow module and single‐samples gene‐set enrichment analysis of...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Yejinpeng Wang, Liang Chen, Gang Wang, Songtao Cheng, Kaiyu Qian, Xuefeng Liu, Chin ‐Lee Wu, Yu Xiao, Xinghuan Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen ‐induced acute liver injury in mice
Our data provided a comprehensive report on the profile and dynamic changes of the liver proteins in AILI; the involvement of Gzmf and the role of Stat3 in necrosis were revealed; and the role of hepatocyte in liver self ‐repair was well clarified. AbstractAcetaminophen (APAP) overdose ‐induced acute liver injury (AILI) is a significant clinical problem worldwide, the hepatotoxicity mechanisms are well elucidated, but the factors involved in the necrosis and repair still remain to be investigated. APAP was injected intraperitoneally in male Institute of Cancer Research (ICR) mic e. Quantitative proteome analysis of liv...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Qin Feng, Ningwei Zhao, Wenkai Xia, ChengJie Liang, Guoxin Dai, Jian Yang, Jingxia Sun, Lanying Liu, Lan Luo, Jie Yang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Ectopic nerve growth factor prevents proliferation in glioma cells by senescence induction
ConclusionsThese findings provide the first evidence that NGF can induce senescence of PLGG cells in a receptor ‐mediated fashion, thus supporting the hypothesis that in the clinical setting NGF might be beneficial to pediatric glioma patients. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Daniela Meco, Angela Maria Di Francesco, Linda Melotti, Antonio Ruggiero, Riccardo Riccardi Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Overexpression of novel lncRNA NLIPMT inhibits metastasis by reducing phosphorylated glycogen synthase kinase 3 β in breast cancer
A novel long noncoding RNA was found to inhibit mammary cell proliferation and motility. Inhibition of glycogen synthase kinase 3 β (GSK3β) activity restored novel lncRNA inhibiting proliferation and metastasis‐induced repression. AbstractLong noncoding RNAs (lncRNAs) are considered as regulators of gene expression in cancers. However, cancer profiling has little focused on noncoding genes. Here, we reported that RP11 –115N4.1 (here renamed novel lncRNA inhibiting proliferation and metastasis [NLIPMT]) was downregulated in breast cancer tissues. Ectopic expression of NLIPMT inhibited mammary cell prolifer...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Yang Jiang, Lili Lin, Shen Zhong, Yangjun Cai, Fen Zhang, Xiaobo Wang, Rongrong Miao, Baodan Zhang, Shenmeng Gao, Xiaoqu Hu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A β stimulates microglial activation through antizyme‐dependent downregulation of ornithine decarboxylase
In this study, we investigated the role of Aβ in mediating microglial activation and polarization both in vitro and in vivo. Our results demonstrated that under the Aβ treatment, ornithine decarboxylase (ODC), a rate‐limiting enzyme in the regulation of arginine catabolism, regulates microglial activation by altering the antizyme (AZ) + 1 ribosomal frameshift. Furthermore, the restoration of ODC protein expression levels has profound effects on inhibition of Aβ‐induced M1 markers and thus attenuates microglial‐mediated cytotoxicity. Altogether, our findings suggested that Aβ may cont...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Yu ‐Wen Cheng, Chun‐Cheng Chang, Ti‐Sheng Chang, Hsin‐Hua Li, Hui‐Chih Hung, Guang‐Yaw Liu, Chih‐Li Lin Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The roles of moonlight ribosomal proteins in the development of human cancers
Moonlighting ribosomal proteins are constituents of ribosomes with important extra ‐ribosomal functions, the implications of which in the initiation and progression of human cancers have been demonstrated. The present review focuses on the role of key moonlight ribosomal proteins in the development of human cancers and the related molecular mechanisms. Abstract“Moonlighting protein” is a term used to define a single protein with multiple functions and different activities that are not derived from gene fusions, multiple RNA splicing, or the proteolytic activity of promiscuous enzymes. Different proteinous con...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Ghader Molavi, Nasser Samadi, Elaheh Zadeh Hosseingholi Tags: REVIEW ARTICLE Source Type: research

Interleukin ‐37 inhibits osteoclastogenesis and alleviates inflammatory bone destruction
Interleukin 37 downregulates osteoclastogenesis through direct and indirect actions AbstractExcessive osteoclast formation is one of the important pathological features of inflammatory bone destruction. Interleukin ‐37 (IL‐37) is an anti‐inflammatory agent that is present throughout the body, but it displays low physiological retention. In our study, high levels of the IL‐37 protein were detected in clinical specimens from patients with bone infections. However, the impact of IL‐37 on osteoclast form ation remains unclear. Next, IL‐37 alleviated the inflammatory bone destruction in the mouse in vivo. We used re...
Source: Journal of Cellular Physiology - November 10, 2018 Category: Cytology Authors: Ruohui Tang, Jin Yi, Jing Yang, Yueqi Chen, Wei Luo, Shiwu Dong, Jun Fei Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The role of nervous system in adaptive response of bone to mechanical loading
The role of sensory and sympathetic nervous system in bone remodeling under mechanical loading. AbstractBone tissue is remodeled through the catabolic function of the osteoclasts and the anabolic function of the osteoblasts. The process of bone homeostasis and metabolism has been identified to be co ‐ordinated with several local and systemic factors, of which mechanical stimulation acts as an important regulator. Very recent studies have shown a mutual effect between bone and other organs, which means bone influences the activity of other organs and is also influenced by other organs and syst ems of the body, especially ...
Source: Journal of Cellular Physiology - November 9, 2018 Category: Cytology Authors: Yini Qiao, Yang Wang, Yimei Zhou, Fulin Jiang, Tu Huang, Liujing Chen, Jingxiang Lan, Cai Yang, Yutong Guo, Shanyu Yan, Zhangming Wei, Juan Li Tags: MINI ‐REVIEW Source Type: research

Function and regulation of apelin/APJ system in digestive physiology and pathology
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 3, 2018 Category: Cytology Authors: Zhen Huang, Xuling Luo, Meiqing Liu, Linxi Chen Source Type: research

Expression of novel proteins by polyomaviruses and recent advances in the structural and functional features of agnoprotein of JC virus, BK virus, and simian virus 40
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 3, 2018 Category: Cytology Authors: A. Sami Saribas, Pascale Coric, Serge Bouaziz, Mahmut Safak Source Type: research

The antiproliferative effects of cold atmospheric plasma ‐activated media on different cancer cell lines, the implication of ozone as a possible underlying mechanism
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 2, 2018 Category: Cytology Authors: Hesam Mokhtari, Leila Farahmand, Kiomars Yaserian, Neda Jalili, Keivan Majidzadeh ‐A Source Type: research

Decellularized breast matrix as bioactive microenvironment for in vitro three ‐dimensional cancer culture
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 2, 2018 Category: Cytology Authors: Qin Jin, Gang Liu, Shubin Li, Haihan Yuan, Zhizhong Yun, Wenqi Zhang, Shu Zhang, Yanfeng Dai, Yuzhen Ma Source Type: research

Repression of TXNIP –NLRP3 axis restores intestinal barrier function via inhibition of myeloperoxidase activity and oxidative stress in nonalcoholic steatohepatitis
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 2, 2018 Category: Cytology Authors: Ru ‐Xue Bai, Ying‐Ying Xu, Geng Qin, Yan‐Ming Chen, Hui‐Fen Wang, Miao Wang, Shi‐Yu Du Source Type: research

Therapeutic potential of human mesenchymal stem cells derived beta cell precursors on a nanofibrous scaffold: An approach to treat diabetes mellitus
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 2, 2018 Category: Cytology Authors: Elham Hoveizi, Shima Tavakol Source Type: research

PU.1 and epigenetic signals modulate 1,25 ‐dihydroxyvitamin D3 and C/EBPα regulation of the human cathelicidin antimicrobial peptide gene in lung epithelial cells
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 2, 2018 Category: Cytology Authors: Ran Wei, Puneet Dhawan, Robert A. Baiocchi, Ki ‐Yoon Kim, Sylvia Christakos Source Type: research

Thymoquinone ‐induced antitumor and apoptosis in human lung adenocarcinoma cells
Journal of Cellular Physiology, EarlyView. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 2, 2018 Category: Cytology Authors: Saeed Samarghandian, Mohsen Azimi ‐Nezhad, Tahereh Farkhondeh Source Type: research