Extended proliferation of chicken ‐ and Okinawa rail‐derived fibroblasts by expression of cell cycle regulators
AbstractAlthough immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian ‐derived cells. We introduced the simian virus 40 T antigen (SV40T) and human papillomavirus(HPV) ‐E6E7 to chick and Okinawa rail (endangered species)derived fibroblast. As a result, neither theSV40T norE6E7 genes could induce avian cell immortality. Accordingly, we attempted to use a recently developed immortalization method, which involved the coexpression of mutant cyclin ‐dependent kinase 4 (CDK4), Cyclin D, an...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Masafumi Katayama, Tohru Kiyono, Hitomi Ohmaki, Takahiro Eitsuka, Daiji Endoh, Miho Inoue ‐Murayama, Nobuyoshi Nakajima, Manabu Onuma, Tomokazu Fukuda Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A review of the anti ‐inflammatory properties of antidiabetic agents providing protective effects against vascular complications in diabetes
AbstractThe global prevalence of Type 2 diabetes mellitus and its associated complications are growing rapidly. Although the role of hyperglycemia is well recognized in the pathophysiology of diabetic complications, its exact underlying mechanisms are not fully understood. In this regard, accumulating evidence suggests that the role of inflammation appears pivotal, with studies showing that most diabetic complications are associated with an inflammatory response. Several classes of antidiabetic agents have been introduced for controlling glycemia, with evidence that these pharmacological agents may have modulatory effects ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Habib Yaribeygi, Stephen L. Atkin, Matteo Pirro, Amirhossein Sahebkar Tags: REVIEW ARTICLE Source Type: research

Interleukin ‐18 and diabetic nephropathy: A review
AbstractThe inflammatory response has an important role in the pathophysiology of diabetic nephropathy that is contributed to by inflammatory mediators such as interleukin ‐1 (IL‐1), IL‐6, IL‐18, tumor necrosis factor‐α, and macrophage chemotactic protein‐1; however, the role of IL‐18 seems to be more specific than other cytokines in the inflammatory process. IL‐18 is expressed in renal tissue and is upregulated by several stimuli including hyperglyce mia. The expression/urinary level of IL‐18 is positively correlated with the progression of diabetic nephropathy and the urinary albumin excretion rate. In...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Habib Yaribeygi, Stephen L. Atkin, Amirhossein Sahebkar Tags: REVIEW ARTICLE Source Type: research

Inactivation of ADAMTS18 by aberrant promoter hypermethylation contribute to lung cancer progression
Our results demonstrated that the tumor suppressor gene ADAMTS18 was downregulated in lung cancer by promoter CpG methylation, and it promoted sensitivity to cisplatin via epidermal growth factor receptor/protein kinase B signaling. Our study suggests that ADAMTS18 promoter methylation is a potential epigenetic biomarker for early detection of lung cancer and warrants investigation as therapeutic target for early ‐stage lung cancer. AbstractLung cancer is the most frequently diagnosed cancer worldwide. Epigenetic regulation contributes to lung cancer pathogenesis. TheADAMTS18 tumor suppressor gene is inactivated in some ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Yan Zhang, Hongying Xu, Junhao Mu, Shuliang Guo, Lin Ye, Dairong Li, Weiyan Peng, Xiaoqian He, Tingxiu Xiang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Noncoding transcribed ultraconserved region (T ‐UCR) UC.48+ is a novel regulator of high‐fat diet induced myocardial ischemia/reperfusion injury
High ‐fat diet (HFD) is an independent risk factor for myocardial ischemia/reperfusion (MI/R) injury. Uc.48+ is a long noncoding RNA (lncRNA) from a transcribed ultraconserved region (T‐UCR) of human, mouse, and rat genomes. Here, we explored the aggravating role of uc.48+ and identified purinerg ic P2X7 receptor (P2X7R) as a downstream regulator of uc.48+ in HFD‐induced MI/R vulnerability. Targeting uc.48+ may be a novel therapeutic approach of MI/R vulnerability to HFD. AbstractIncreasing evidence has suggested high ‐fat diet (HFD) is an independent risk factor for myocardial ischemia/reperfusion (MI/R) i...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Lu Ding, Chengxin Gong, Jiani Zhao, Xingzi Liu, Tao Li, Shenqiang Rao, Shuo Wang, Yuanyuan Liu, Shanping Peng, Wen Xiao, Chaopeng Xiong, Rumeng Wang, Shangdong Liang, Hong Xu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Understanding molecular biology of codon usage in mitochondrial complex IV genes of electron transport system: Relevance to mitochondrial diseases
The mitochondrial cytochrome oxidase (CO) genes are involved in complex IV of the electron transport system (ETS), and dysfunction of CO genes leads to several diseases.  nHowever, no work has been reported on the codon usage pattern of these genes. We used bioinformatic methods to analyze the compositional properties and the codon usage pattern of the COI, COII, and COIII genes in fishes, birds, and mammals to understand the similarities and dissimilarities of cod on usage in these genes, which gave an insight into the molecular biology of these genes. AbstractThe mitochondrial cytochrome oxidase (CO) genes are involved ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Arif Uddin, Tarikul Huda Mazumder, Supriyo Chakraborty Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

CircRNA circPDSS1 promotes the gastric cancer progression by sponging miR ‐186‐5p and modulating NEK2
This study mainly researched the effects of circPDSS1 and its related genes in gastric cancer (GC). 2.It revealed part of a complex regulation system, circPDSS1/miR ‐186‐5p/NEK2 axis in GC, and provided a potential therapeutic target for GC treating. 3.Further research should be done to investigate the related genes in this regulation system. AbstractThe aim of this study is to investigate the regulatory mechanism of circPDSS1/miR ‐186‐5p/NEK2 axis on the viability and proliferation in gastric cancer (GC) cell line. Differentially expressed circRNAs, miRNAs, and mRNAs in GC tissues and paracarcinoma tissues were ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Yiming Ouyang, Yuejin Li, Yingguang Huang, Xing Li, Yu Zhu, Yaxin Long, Yongzhi Wang, Xiaodong Guo, Kunmei Gong Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The role of DEAD ‐box RNA helicase p68 (DDX5) in the development and treatment of breast cancer
Discussion of the precise role of p68 in cancer is complex and depends on the cellular microenvironment and interacting factors. In terms of the deregulated expression of p68 in breast cancer and the high prevalence of this cancer among women, it can be informative to review the precise function of this factor in the breast cancer. Therefore, an attempt will be made in this review to clarify the tumorigenic function of p68 in association with its targeting potential for the treatment of breast cancer. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Vida Hashemi, Ali Masjedi, Bita Hazhir ‐karzar, Asghar Tanomand, Siamak Sandoghchian Shotorbani, Mohammad Hojjat‐Farsangi, Ghasem Ghalamfarsa, Gholamreza Azizi, Enayat Anvari, Behzad Baradaran, Farhad Jadidi‐Niaragh Tags: REVIEW ARTICLE Source Type: research

Mitochondrial quality control in cardiac cells: Mechanisms and role in cardiac cell injury and disease
Parkin ‐mediated mitophagy in Drosophila heart. (a) Parkin deficiency in Drosophila heart impaired mitophagy and led to the development of dilated cardiomyopathy. (b) Simultaneous deletion of mitochondrial assembly regulatory factor and Parkin rescued cardiomyopathy (Bhandari et al.,#jcp27597-bib-0005).#jcp27597-bib-0005 AbstractMitochondria play an important role in maintaining cardiac homeostasis by supplying the major energy required for cardiac excitation –contraction coupling as well as controlling the key intracellular survival and death pathways. Healthy mitochondria generate ATP molecules through an aerobic pro...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Farzaneh G. Tahrir, Dianne Langford, Shohreh Amini, Taha Mohseni Ahooyi, Kamel Khalili Tags: REVIEW ARTICLE Source Type: research

Estrogen deprivation aggravates intracellular calcium dyshomeostasis in the heart of obese ‐insulin resistant rats
Estrogen deprivation had a strong impact on abnormal cardiac function through intracellular calcium regulation. Our findings showed that in obese ‐insulin resistant rats, estrogen deprivation severely aggravates cardiac dysfunction via increased impairment of calcium homeostasis. AbstractThe incidence of cardiovascular disease and metabolic syndrome increases after the onset of menopause, giving evidence for the vital role of estrogen. Intracellular calcium [Ca2+]i regulation plays an important role in the maintenance of left ventricular (LV) contractile function. Although either estrogen deprivation or obesity has been ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Siripong Palee, Wanitchaya Minta, Duangkamol Mantor, Wissuta Sutham, Sasiwan Kerdphoo, Wasana Pratchayasakul, Siriporn C. Chattipakorn, Nipon Chattipakorn Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A2a adenosine receptor agonist improves endoplasmic reticulum stress in MIN6 cell line through protein kinase A/ protein kinase B/ Cyclic adenosine monophosphate response element ‐binding protein/ and Growth Arrest And DNA‐Damage‐Inducible 34/ eukaryotic Initiation Factor 2α pathways
A2a adenosine receptor activation improved endoplasmic reticulum (ER) stress in pancreatic beta cells (PBCs) and its signaling may provide new approach to control ER stress in the PBC cells. AbstractEndoplasmic reticulum (ER) stress is one of the main molecular events underlying pancreatic beta cell (PBC) failure, apoptosis, and a decrease in insulin secretion. Recent studies have highlighted the fundamental role of A2a adenosine receptor (A2aR) in potentiation of insulin secretion and proliferation of PBCs. However, possible protective effects of A2aR signaling against ER stress have not been elucidated yet. Thus, in the ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Fatemeh P. Arasi, Mohammad K. Shahrestanaki, Mahmoud Aghaei Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

MiRNAs and inflammatory bowel disease: An interesting new story
MicroRNAs (miRNAs) are a set of noncoding RNA molecules that are involved in the regulation of the gene expression. We discussed the critical roles of some miRNAs in the pathophysiology, diagnosis, and treatment of IBD. AbstractInflammatory bowel disease (IBD), as a chronic and recurrent inflammatory disorder, is caused by a dysregulated and aberrant immune response to exposed environmental factors in genetically susceptible individuals. Despite huge efforts in determining the molecular pathogenesis of IBD, an increasing worldwide incidence of IBD has been reported. MicroRNAs (miRNAs) are a set of noncoding RNA molecules t...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Soheila Moein, Mostafa Vaghari ‐Tabari, Durdi Qujeq, Maryam Majidinia, Seyed Mohammad Nabavi, Bahman Yousefi Tags: REVIEW ARTICLE Source Type: research

Effects of microRNA ‐217 on proliferation, apoptosis, and autophagy of hepatocytes in rat models of CCL4‐induced liver injury by targeting NAT2
This study aims to explore the effects of miR ‐217 targeting NAT2 on hepatocyte proliferation, apoptosis, and autophagy following carbon tetrachloride (CCL4)‐induced liver injury. Rat models of CCL4‐induced liver injury were established. Healthy Wistar rats were randomized into the normal, blank, negative control (NC), microRNA‐217 (miR ‐217) mimic, miR‐217 inhibitor, small interfering RNA (siRNA)‐N‐acetyltransferase 2 (NAT2), and miR‐217 inhibitor + siRNA‐NAT2 groups. NAT2 activity was evaluated with reversed‐phase high‐performance liquid chromatographic method. Immunohistochemistry was used t...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Cheng ‐Liang Yang, Xiao‐Li Zheng, Ke Ye, Ya‐Nan Sun, Yu‐Fei Lu, Hong Ge, Hui Liu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Inflammation and pericarditis: Are neutrophils actors behind the scenes?
The morbidity of acute pericarditis is increasing overtime. Apart the known impairment of the adaptive immunity, recently a large body evidence indicated the central role of the innate immune system in the pathogenesis of recurrent pericarditis, starting from similarities with autoinflammatory diseases, with the “inflammasome” playing an important role. The role of the adaptive immune system in pericarditis cannot be reduced to a black or white issue as these mechanisms often overlap and new therapeutic strategies should be investigated in this direction. AbstractThe morbidity of acute pericarditis is increasing over t...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Aldo Bonaventura, Fabrizio Montecucco Tags: MINI ‐REVIEW Source Type: research

Exosomes derived from acute myeloid leukemia cells promote chemoresistance by enhancing glycolysis ‐mediated vascular remodeling
AbstractAcute myeloid leukemia (AML) is the most common type of leukemia in adults. AML cells secrete angiogenic factors to remodel vasculature and acquire chemoresistance; however, antiangiogenic drugs are often ineffective in AML treatment. Cancer cell ‐derived exosomes can induce angiogenesis, but their role in vascular remodeling during AML is unclear. Here, we found that exosomes secreted by AML cells promoted proliferation and migration and tube‐forming activity of human umbilical vein endothelial cells (HUVECs), whereas HUVECs conferred c hemoresistance to AML cells. AML cell‐derived exosomes contained vascula...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Bin Wang, Xiaoting Wang, Diyu Hou, Qian Huang, Weiwu Zhan, Canwei Chen, Jingru Liu, Ruolan You, Jieqiong Xie, Ping Chen, Huifang Huang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research