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Different behaviour of DVL1, DVL2, DVL3 in astrocytoma malignancy grades and their association to TCF1 and LEF1 upregulation
AbstractKey regulators of the Wnt signalling, DVL1, DVL2 and DVL3, in astrocytomas of different malignancy grades were investigated. Markers forDVL1, DVL2 andDVL3 were used to detect microsatellite instability (MSI) and gross deletions (LOH), while immunohistochemistry and immunoreactivity score were used to determine the signal strengths of the three DVL proteins and transcription factors of the pathway, TCF1 and LEF1. Our findings demonstrated that MSI at all threeDVL loci was constantly found across tumour grades with the highest number in grade II (P = 0.008). Collectively, LOHs were more frequent in high‐grade tum...
Source: Journal of Cellular and Molecular Medicine - November 23, 2018 Category: Molecular Biology Authors: Anja Kafka, Mateja Ba čić, Davor Tomas, Kamelija Žarković, Anja Bukovac, Niko Njirić, Goran Mrak, Željka Krsnik, Nives Pećina‐Šlaus Tags: ORIGINAL ARTICLE Source Type: research

LncRNA MEG3 inhibits the progression of prostate cancer by modulating miR ‐9‐5p/QKI‐5 axis
This study was designed to detecting the influences of lncRNA MEG3 in prostate cancer. Aberrant lncRNAs expression profiles of prostate cancer were screened by microarray analysis. The qRT ‐PCR and Western blot were employed to investigating the expression levels of lncRNA MEG3, miR‐9‐5p andQKI ‐5. The luciferase reporter assay was utilized to testifying the interactions relationship among these molecules. Applying CCK ‐8 assay, wound healing assay, transwell assay and flow cytometry in turn, the cell proliferation, migration and invasion abilities as well as apoptosis were measured respectively. LncRNA MEG3 was ...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Meng Wu, Yawei Huang, Tongchang Chen, Weichao Wang, Shiguang Yang, Zhenfeng Ye, Xiaoqing Xi Tags: ORIGINAL ARTICLE Source Type: research

Gliomasphere marker combinatorics: multidimensional flow cytometry detects CD44+/CD133+/ITGA6+/CD36+ signature
AbstractGlioblastoma is the most dangerous brain cancer. One reason for glioblastoma's aggressiveness are glioblastoma stem ‐like cells. To target them, a number of markers have been proposed (CD133, CD44, CD15, A2B5, CD36, CXCR4, IL6R, L1CAM, and ITGA6). A comprehensive study of co‐expression patterns of them has, however, not been performed so far. Here, we mapped the multidimensional co‐expression profile of the se stemness‐associated molecules. Gliomaspheres – an established model of glioblastoma stem‐like cells – were used. Seven different gliomasphere systems were subjected to multicolor flow cytometry ...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Friedrich Erhart, Bernadette Blauensteiner, Gabriel Zirkovits, Dieter Printz, Klara Soukup, Simone Klingenbrunner, Katrin Fischhuber, Ren é Reitermaier, Angela Halfmann, Daniela Lötsch, Sabine Spiegl‐Kreinecker, Walter Berger, Carmen Visus Tags: ORIGINAL ARTICLE Source Type: research

Testin protects against cardiac hypertrophy by targeting a calcineurin ‐dependent signalling pathway
This study investigated the influence of TES on cardiac hypertrophy and its etiology. Murine models with excessive TES expression specific to the heart were constructed with an adeno ‐associated virus expression system. Cardiac hypertrophy was stimulated through aortic banding (AB). The severity of cardiac hypertrophy was evaluated through molecular, echocardiographic, pathological, and hemodynamic examination. The findings of our study revealed that TES expression was remarka bly suppressed not only in failing human hearts but also in mouse hearts with cardiac hypertrophy. It was discovered that excessive TES expression...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Lu Gao, Yuan Liu, Sen Guo, Lili Xiao, Cui Liang, Xiaofang Wang Tags: ORIGINAL ARTICLE Source Type: research

MicroRNA let ‐7a regulates angiogenesis by targeting TGFBR3 mRNA
AbstractAngiogenesis has a great impact on human health, owing to its participation in development, wound healing and the pathogenesis of several diseases. It has been reported that let ‐7a is a tumour suppressor, but whether it plays a role in angiogenesis is unclear. Here we showed that let‐7a, a microRNA conserved in vertebrates, regulated angiogenesis by concomitantly down‐regulating TGFBR3. Overexpression of let‐7a or knockdown of TGFBR3 in cell culture inhibited the t ube formation and reduced migration rate. Moreover, xenograft experiments showed that overexpression of let‐7a or knockdown of TGFBR3 had sma...
Source: Journal of Cellular and Molecular Medicine - November 22, 2018 Category: Molecular Biology Authors: Shao Wang, Huandong Zhou, Dazhou Wu, Huajing Ni, Zhongliang Chen, Chengshui Chen, Youqun Xiang, Kezhi Dai, Xiaoming Chen, Xi Li Tags: ORIGINAL ARTICLE Source Type: research

Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors
In this study, we extended our analysis of the molecular effects of romidepsin to deepen our understanding of the underlying mechanisms. P atients will benefit from these analyses, since detailed knowledge of the romidepsin effects allows for a better risk and side‐effect assessment. We screened for changes in histone acetylation of specific lysine residues and analysed changes in the DNA methylation landscape after romidepsin treatm ent of the GCT cell lines TCam‐2, 2102EP, NCCIT and JAR, while human fibroblasts were used as controls. In addition, we focused on the role of the dehydrogenase/reductaseDHRS2, which was s...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Daniel Nettersheim, Daniel Berger, Sina Jostes, Margaretha Skowron, Hubert Schorle Tags: ORIGINAL ARTICLE Source Type: research

The variant at TGFBRAP1 is significantly associated with type 2 diabetes mellitus and affects diabetes ‐related miRNA expression
AbstractWhile the transforming growth factor ‐β1 (TGF‐β1) regulates the growth and proliferation of pancreatic β‐cells, its receptors trigger the activation of Smad network and subsequently induce the insulin resistance. A case‐control was conducted to evaluate the associations of the polymorphisms of TGF‐β1 receptor‐associated protein 1 (TGFBRAP1) and TGF ‐β1 receptor 2 (TGFBR2) with type 2 diabetes mellitus (T2DM), and its genetic effects on diabetes ‐related miRNA expression. miRNA microarray chip was used to screen T2DM‐related miRNA and 15 differential expressed miRNAs were further validated in ...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Song Yang, Xiaotian Chen, Mengyao Yang, Xianghai Zhao, Yanchun Chen, Hailong Zhao, Chunlan Liu, Chong Shen Tags: ORIGINAL ARTICLE Source Type: research

Pancreatic fibroblast growth factor 21 protects against type 2 diabetes in mice by promoting insulin expression and secretion in a PI3K/Akt signaling ‐dependent manner
In this study, we found that FGF21 was down ‐regulated in pancreatic islets of db/db mice, a mouse model of T2DM, along with decreased insulin expression, suggesting the possible involvement of FGF21 in maintaining insulin homeostasis and islet β‐cell function. Importantly, FGF21 knockout exacerbated palmitate‐induced islet β‐cell f ailure and suppression of glucose‐stimulated insulin secretion (GSIS). Pancreatic FGF21 overexpression significantly increased insulin expression, enhanced GSIS, improved islet morphology and reduced β‐cell apoptosis in db/db mice. Mechanistically, FGF21 promoted expression of in...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Yingying Pan, Baile Wang, Jujia Zheng, Rongrong Xiong, Zhichao Fan, Yanna Ye, Saisai Zhang, Qinyao Li, Fanghua Gong, Chaoming Wu, Zhuofeng Lin, Xiaokun Li, Xuebo Pan Tags: ORIGINAL ARTICLE Source Type: research

Novel peptide dermaseptin ‐PS1 exhibits anticancer activity via induction of intrinsic apoptosis signalling
In this study, a novel AMP named Dermaseptin ‐PS1 was isolated and identified fromPhyllomedusa sauvagei, it showed strong antimicrobial activities against three types of microorganisms. In vitro antiproliferative studies on human glioblastoma U ‐251 MG cells indicated that Dermaseptin‐PS1 disrupted cell membranes at the concentrations of 10−5 M and above, while the cell membrane integrity was not affected when concentrations were decreased to 10−6 M or lower. Further examinations revealed that, at the relatively low concentration (10−6 M), Dermaseptin‐PS1 induced apoptosis through mitochondrial‐related s...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Qilin Long, Lei Li, Hao Wang, Miaoran Li, Lei Wang, Mei Zhou, Qiaozhu Su, Tianbao Chen, Yuxin Wu Tags: ORIGINAL ARTICLE Source Type: research

Targeting PRMT5/Akt signalling axis prevents human lung cancer cell growth
AbstractThe emerging evidence reveals that protein arginine methyltransferase 5 (PRMT5) is involved in regulation of tumour cell proliferation and cancer development. Nevertheless, the exact role of PRMT5 in human lung cancer cell proliferation and the underlying molecular mechanism remains largely obscure. Here, we showed that PRMT5 was highly expressed in human lung cancer cells and lung cancer tissues. Furthermore, we generated PRMT5 stable knockdown cell lines (A549 and H1299 cells) and explored the functions of PRMT5 in lung cancer cell proliferation. We found that the down ‐regulation of PRMT5 by shRNA or the inhib...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Shikui Zhang, Yaqiong Ma, Xiaoyan Hu, Yonghua Zheng, Xiaoke Chen Tags: ORIGINAL ARTICLE Source Type: research

Small molecule nAS ‐E targeting cAMP response element binding protein (CREB) and CREB‐binding protein interaction inhibits breast cancer bone metastasis
In this study, we tested the effect of nAS‐E on breast cancer cell proliferation, survival, migration as well as osteoclast formation and bone resorption in vitro for the first time. Our results demonstrated that nAS‐E inh ibited breast cancer cell proliferation, migration, survival and suppressed osteoclast differentiation as well as bone resorption through inhibiting CREB‐CBP interaction. In addition, the in vivo effect of nAS‐E in protecting against breast cancer‐induced osteolysis was evaluated. Our results indicated that nAS‐E could reverse bone loss induced by MDA‐MB‐231 tumour. These results suggest ...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Min Jiang, Yufei Yan, Kai Yang, Zhuochao Liu, Jin Qi, Hanbing Zhou, Niandong Qian, Qi Zhou, Tianqi Wang, Xing Xu, Xiangshu Xiao, Lianfu Deng Tags: ORIGINAL ARTICLE Source Type: research

Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
AbstractWe evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS ‐Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS‐Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS‐Ex than Sham. Myocyte diameter and interstit...
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: David R. A. Reyes, Mariana J. Gomes, Camila M. Rosa, Luana U. Pagan, Silmeia G. Zanati, Ricardo L. Damatto, Eder A. Rodrigues, Robson F. Carvalho, Ana A. H. Fernandes, Paula F. Martinez, Aline R. R. Lima, Marcelo D. M. Cezar, Luiz E. F. M. Car Tags: ORIGINAL ARTICLE Source Type: research

Autocrine VEGF signalling on M2 macrophages regulates PD ‐L1 expression for immunomodulation of T cells
In conclusion, our findings provide a novel biological basis to support the current successful strategy using combined VEGF/PD ‐1 signalling blockade in cancer therapy. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Yin ‐Siew Lai, Rika Wahyuningtyas, Shin‐Peir Aui, Ko‐Tung Chang Tags: ORIGINAL ARTICLE Source Type: research

Tongguan capsule ‐derived herb reduces susceptibility to atrial fibrillation by inhibiting left atrial fibrosis via modulating cardiac fibroblasts
In conclusion, the current study demonstrated that TGD reduces susceptibility to AF and improves LA conduction function in rats with p ost‐MI by inhibiting left atrial fibrosis and modulating CFs. Targeting the CF population may be a novel antiarrhythmic therapeutic approach. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Shiyu Ma, Jin Ma, Liheng Guo, Junqi Bai, Shuai Mao, Minzhou Zhang Tags: ORIGINAL ARTICLE Source Type: research

Age ‐related ultrastructural changes of the basement membrane in the mouse blood‐brain barrier
AbstractThe blood ‐brain barrier (BBB) is essential for a functional neurovascular unit. Most studies focused on the cells forming the BBB, but very few studied the basement membrane (BM) of brain capillaries in ageing. We used transmission electron microscopy and electron tomography to investigate the BM of the BB B in ageing C57BL/6J mice. The thickness of the BM of the BBB from 24‐month‐old mice was double as compared with that of 6‐month‐old mice (107 nm vs 56 nm). The aged BBB showed lipid droplets gathering within the BM which further increased its thickness (up to 572 nm) and altered its stru cture. The...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Laura Cristina Ceafalan, Tudor Emanuel Fertig, Teodora Cristina Gheorghe, Mihail Eugen Hinescu, Bogdan Ovidiu Popescu, Jens Pahnke, Mihaela Gherghiceanu Tags: ORIGINAL ARTICLE Source Type: research