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Characterization of a novel carboxylesterase from Bacillus velezensis SYBC H47 and its application in degradation of phthalate esters
This study also proved that BaCEs04 could be used as an ideal candidate for the application in bioremediation and industry.Graphical abstract (Source: Journal of Bioscience and Bioengineering)
Source: Journal of Bioscience and Bioengineering - November 22, 2019 Category: Biomedical Science Source Type: research

Fluorescent detection of nisin by genetically modified Lactococcus lactis strains in milk and a colonic model: Application of whole-cell nisin biosensors
In this study, we developed two new bioreporters for nisin based on Lactococcus lactis NZ9000 transformed with the vector pNZ:Nis-aFP or pNZ:Nis-mCherry, that encoded for the anaerobic fluorescent protein evoglow-Pp1 (aFP) or the fluorescent protein mCherry, respectively. The biosensors were used to study nisin A production by L. lactis INIA 650 in milk and in a colonic model. The use of L. lactis NZ9000 pNZ:Nis-aFP as a biosensor allowed the detection of nisin produced by L. lactis INIA 650 in milk, but not in the in vitro colonic model. In milk, this reporter was induced by direct addition of 10 ng/ml nisin while, i...
Source: Journal of Bioscience and Bioengineering - November 20, 2019 Category: Biomedical Science Source Type: research

Screening and isolation of the liamocin-producing yeast Aureobasidium melanogenum using xylose as the sole carbon source
In this study, we screened and isolated yeast strains that synthesize BSs using xylose as the sole carbon source. We applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to screen for BS-producing yeasts and isolated eight strains as the liamocin producers. Two of the eight strains, AS37 and SK25, were identified as Aureobasidium melanogenum, which is known as black yeasts, by based on 26S ribosomal RNA gene sequences. Both strains produced a wide variety of liamocin structures from not only xylose but also glucose and sucrose. According to the MALDI-TOF MS analysis, signals c...
Source: Journal of Bioscience and Bioengineering - November 13, 2019 Category: Biomedical Science Source Type: research

Disruption of thin- and thick-wall microalgae using high pressure gases: Effects of gas species, pressure and treatment duration on the extraction of proteins and carotenoids
Publication date: Available online 12 November 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Tze Ching Yong, Pai-Ho Chiu, Chi-Hui Chen, Chun-Hung Hung, Ching-Nen Nathan ChenIndustrial scale microalgal cell disruption requires low cost, high efficiency and structural conservation of biomolecules for biorefinery. Many cultivated microalgae have thick walls and these walls are barriers for efficient cell disruption. Until recently, despite the high biodiversity of microalgae, little attention has been paid to thin-wall microalgal species in the natural environment for the production and recovery of valuable b...
Source: Journal of Bioscience and Bioengineering - November 13, 2019 Category: Biomedical Science Source Type: research

Manufactural impact of the solid-state saccharification process in rice-flavor baijiu production
Publication date: Available online 9 November 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Xuan Yin, Yumiko Yoshizaki, Makoto Ikenaga, Xing-Lin Han, Kayu Okutsu, Taiki Futagami, Hisanori Tamaki, Kazunori TakamineRice-flavor baijiu is a traditional Chinese liquor that is manufactured using a solid-state saccharification process. In the present study, we investigated the role of the process in making of rice-flavor baijiu using chemical and biological quantitative analysis approaches. More than 70% of starch in rice decomposed to glucose after saccharification. In addition, the number of fungal cells, sacch...
Source: Journal of Bioscience and Bioengineering - November 10, 2019 Category: Biomedical Science Source Type: research

Production and characterization of a recombinant thermophilic trehalose synthase from Thermus antranikianii
In this study, we synthesized a thermophilic trehalose synthase from Thermus antranikianii (TaTS), which was recombinantly expressed in Escherichia coli BL21(DE3). The recombinant TaTS showed the highest activity at pH 7.0 and 60°C, with the maximum trehalose yield (76.8%) obtained at pH 7.0 and 30°C. TaTS activity was stable over a wide pH and temperature range of 6–10 and 4–70°C, respectively, over 6 h of incubation. The enzyme activity was strongly inhibited by Co2+, Cu2+, Zn2+, sodium dodecyl sulfate, and Tris. TaTS showed a 1.48-fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose. Overall...
Source: Journal of Bioscience and Bioengineering - November 9, 2019 Category: Biomedical Science Source Type: research

Biovalorization of soybean residue (okara) via fermentation with Ganoderma lucidum and Lentinus edodes to attain products with high anti-osteoporotic effects
In this study, solid-state fermentation was performed using Ganoderma lucidum and Lentinus edodes. Antioxidant activity and bioactive compound levels in G. lucidum-fermented okara (GLFO) and L. edodes-fermented okara (LEFO) were assayed. Antiosteoporosis bioactivity was evaluated using an animal model. The results demonstrated that solid-state fermentation significantly improved the antioxidant activity and bioactive compound levels. Furthermore, GLFO and LEFO increased trabecular bone volume, although only the GLFO-treated group exhibited significantly improved trabecular separation compared with the bilateral ovariecto...
Source: Journal of Bioscience and Bioengineering - November 9, 2019 Category: Biomedical Science Source Type: research

Response to inhibitory conditions of acetate-degrading methanogenic microbial community
Publication date: Available online 5 November 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Hui-Zhong Wang, Jie Li, Yue Yi, Masaru K. Nobu, Takashi Narihiro, Yue-Qin TangInvestigating the effects of different kinds of inhibitors on the activity and structure of acetate-degrading microbial community involved in methane fermentation is critically important for developing countermeasures to make the fermentation process stable under different inhibitory conditions. In the present study, a mesophilic chemostat fed with acetate as the sole carbon source was constructed. Microbial community analysis based on hig...
Source: Journal of Bioscience and Bioengineering - November 6, 2019 Category: Biomedical Science Source Type: research

Characterization of γ-aminobutyric acid (GABA)-producing Saccharomyces cerevisiae and coculture with Lactobacillus plantarum for mulberry beverage brewing
In conclusion, S. cerevisiae SC125 and L. plantarum BC114 coculture promotes the production of flavor compounds and GABA in mulberry beverage brewing. (Source: Journal of Bioscience and Bioengineering)
Source: Journal of Bioscience and Bioengineering - November 1, 2019 Category: Biomedical Science Source Type: research

Quantification, regulation and production of 5-aminolevulinic acid by green fluorescent protein in recombinant Escherichia coli
Publication date: Available online 31 October 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Shih-I. Tan, Shao-Chun You, I-Tai Shih, I-Son Ng5-Aminolevulinic acid (5-ALA) is an unnatural amino acid and has been approved as a biodegradable, non-toxic pesticide and herbicide with applications in sustainable agriculture. 5-ALA can also be applied for cancer targeting via tumor localization and photodynamic therapy. Herein, we developed a feasible quantification, regulation and production method of 5-ALA in Escherichia coli is based on the chimera of 5-ALA synthetase from Rhodobacter sphaeroides (RshemA) and su...
Source: Journal of Bioscience and Bioengineering - November 1, 2019 Category: Biomedical Science Source Type: research

Novel neuromuscular junction model in 2D and 3D myotubes co-cultured with induced pluripotent stem cell-derived motor neurons
Publication date: Available online 31 October 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Kantaro Yoshioka, Akira Ito, Yoshinori Kawabe, Masamichi KamihiraMotor neurons differentiated from induced pluripotent stem (iPS) cells have attracted attention for use in the construction of drug screening systems for neuronal diseases, such as amyotrophic lateral sclerosis. However, conventional drug screening systems using 2-dimensional (2D) cultures of iPS cell-derived motor neurons often evaluate the cell survival rate, morphological changes in the cells and/or gene expression analysis, and these parameters do ...
Source: Journal of Bioscience and Bioengineering - November 1, 2019 Category: Biomedical Science Source Type: research

Factors affecting levels of ferulic acid, ethyl ferulate and taste-active pyroglutamyl peptides in sake
Publication date: Available online 28 October 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Katsumi Hashizume, Toshihiko Ito, Karin Shirato, Naomi Amano, Tetsuo Tokiwano, Tsuyoshi Ohno, Sho Shindo, Seiei Watanabe, Masaki OkudaFactors affecting ferulic acid, ethyl ferulate and taste-active pyroglutamyl (pGlu) peptides levels in sake were analyzed using small-scale sake brewing tests on eighteen rice samples with differing cultivar variety, cropped year and area, and polishing rate. Ferulic acid concentration in sake was highly positively correlated with its content in rice (r = 0.782**) (double asterisk i...
Source: Journal of Bioscience and Bioengineering - October 29, 2019 Category: Biomedical Science Source Type: research

Enhancement in catalytic activity of CotA-laccase from Bacillus pumilus W3 via site-directed mutagenesis
This study showed that Gly208 and Ala227 play key roles in catalytic efficiency and it is possible to improve catalytic efficiency of CotA-laccase through site-directed mutagenesis. (Source: Journal of Bioscience and Bioengineering)
Source: Journal of Bioscience and Bioengineering - October 29, 2019 Category: Biomedical Science Source Type: research

Liquefaction of porcine hoof shell to prepare peptone substitute by instant catapult steam explosion
Publication date: Available online 22 October 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Qingshan Shen, Hang Wang, Chunhui Zhang, Xiaojie Qin, Wei Jia, Xiong Xu, Aurore Richel, Qiankun ZhengInstant catapult steam explosion (ICSE) was proposed as a method to liquefy porcine hoof shell (PHS) and prepare a peptone substitute for fermentation culture, achieving environmentally friendly animal by-product recycling. The liquefaction of PHS was conducted at various pressures (0.5–2.3 MPa) for 5–30 min. As evidenced by the scanning electron microscopy analysis, ICSE caused randomly cracks changing the mor...
Source: Journal of Bioscience and Bioengineering - October 23, 2019 Category: Biomedical Science Source Type: research

Native display of a huge homotrimeric protein fiber on the cell surface after precise domain deletion
Publication date: Available online 22 October 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Sota Aoki, Shogo Yoshimoto, Masahito Ishikawa, Dirk Linke, Andrei Lupas, Katsutoshi HoriAtaA, a trimeric autotransporter adhesin from Acinetobacter sp. Tol 5, exhibits nonspecific, high adhesiveness to abiotic surfaces. For identification of the functional domains of AtaA, precise design of domain-deletion mutants is necessary so as not to cause undesirable structural distortion. Here, we designed and constructed three types of AtaA mutants from which the same domain, FGG1, was deleted. The first mutant was designed...
Source: Journal of Bioscience and Bioengineering - October 23, 2019 Category: Biomedical Science Source Type: research