Journal of Biomolecular Techniques : JBT This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Article Watch: December, 2023
J Biomol Tech. 2023 Dec 18;34(4):3fc1f5fe.53f1c3bf. doi: 10.7171/3fc1f5fe.53f1c3bf. eCollection 2023 Dec.ABSTRACTThis column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, AU-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Tel; (706) 713-2216: Fax; (706) 713-2221: Email; cslaught@uga.edu or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the Association.PMID:38268996 | PMC:PMC...
Source: Journal of Biomolecular Techniques : JBT - January 25, 2024 Category: Molecular Biology Authors: Clive Slaughter Source Type: research

Expanding a Wastewater-Based Surveillance Methodology for DNA Isolation from a Workflow Optimized for SARS-CoV-2 RNA Quantification
The objective of this study was to establish the necessary steps for isolating DNA from wastewater by modifying a long-standing RNA-specific extraction workflow optimized for SARS-CoV-2 detection. Modifications were made to the sample concentration process and included an evaluation of bead bashing prior to the extraction of either DNA or RNA. Results showed that bead bashing reduced detection of RNA from wastewater but improved recovery of DNA as assessed by quantitative polymerase chain reaction (qPCR). Bead bashing is therefore not recommended for the quantification of RNA viruses using qPCR. Whereas for Mycobacterium b...
Source: Journal of Biomolecular Techniques : JBT - January 25, 2024 Category: Molecular Biology Authors: Kristina M Babler Mark E Sharkey Ayaaz Amirali Melinda M Boone Samuel Comerford Benjamin B Currall George S Grills Jennifer Laine Christopher E Mason Brian Reding Stephan Sch ürer Mario Stevenson Du šica Vidović Sion L Williams Helena M Solo-Gabriele Source Type: research

A Survey on Core Flow Cytometry Facilities: Instrument Maintenance, Usage, and Funding
J Biomol Tech. 2023 Nov 30;34(4):3fc1f5fe.557b2117. doi: 10.7171/3fc1f5fe.557b2117. eCollection 2023 Dec.ABSTRACTFlow cytometry is a powerful tool that finds applications in various fields such as immunology, molecular biology, cancer biology, virology, and infectious disease monitoring. A significant portion of the research in these disciplines is supported by flow cytometry shared resource laboratories (SRLs). There are several types of flow cytometers available for use in SRLs, including analyzers, sorters, imaging flow cytometers, and mass cytometers. Each type has different challenges when it comes to maintenance and ...
Source: Journal of Biomolecular Techniques : JBT - January 25, 2024 Category: Molecular Biology Authors: Sonal Priya Kathleen Brundage Ron Orlando Source Type: research

Elevating the Educational Mission of "Full-Service" Core Facilities through Formal Biotechnology Workshops
J Biomol Tech. 2023 Dec 4;34(4):3fc1f5fe.30644720. doi: 10.7171/3fc1f5fe.30644720. eCollection 2023 Dec.ABSTRACTCore facility laboratories are an essential part of the successful research enterprise of many universities around the world. Core facilities provide state-of-the-art instrumentation and technologies to support research of all faculty, postdocs, and students on a fee-for-service basis. Academic next-generation sequencing cores are typically "full service" facilities, and access to and training on their instrumentation is limited to core staff. To address these limitations, we provided graduate students with techn...
Source: Journal of Biomolecular Techniques : JBT - January 25, 2024 Category: Molecular Biology Authors: Emily Ricco Stacy Nguyen Victoria Rodriguez Kieu Pham Esmanur Tokcan Sherita Love Alana Newell Daniel C Kraushaar Source Type: research