Analysis of Microbiomes from Ultra-Low Biomass Surfaces Using Novel Surface Sampling and Nanopore Sequencing
J Biomol Tech. 2023 Aug 10;34(3):3fc1f5fe.bac4a5b3. doi: 10.7171/3fc1f5fe.bac4a5b3. eCollection 2023 Sep 30.ABSTRACTThe rapid assessment of microbiomes from ultra-low biomass environments such as cleanrooms or hospital operating rooms has a number of applications for human health and spacecraft manufacturing. Current techniques often employ lengthy protocols using short-read DNA sequencing technology to analyze amplified DNA and have the disadvantage of a longer analysis time and lack of portability. Here, we demonstrate a rapid (~24 hours) on-site nanopore-based sequencing approach to characterize the microbiome of a NASA...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Anna C Simpson Scott Tighe Season Wong Patrick Leo Ceth Parker Atul M Chander Michael Williams Hao-Wei Wu Kasthuri Venkateswaran Nitin K Singh Source Type: research

Article Watch: September, 2023
J Biomol Tech. 2023 Sep 25;34(3):3fc1f5fe.bdb707e7. doi: 10.7171/3fc1f5fe.bdb707e7. eCollection 2023 Sep 30.ABSTRACTThis column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, AU-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Tel; (706) 713-2216: Fax; (706) 713-2221: Email; cslaught@uga.edu or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the Association.PMID:37969873 | PMC:...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Clive Slaughter Source Type: research

The Association of Biomolecular Resource Facilities Proteome Informatics Research Group Study on Metaproteomics (iPRG-2020)
This study provides insights into the status of metaproteomics bioinformatics in participating laboratories and core facilities.PMID:37969874 | PMC:PMC10644979 | DOI:10.7171/3fc1f5fe.a058bad4 (Source: Journal of Biomolecular Techniques : JBT)
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Pratik D Jagtap Michael R Hoopmann Benjamin A Neely Antony Harvey Lukas K äll Yasset Perez-Riverol Milky K Abajorga Julie A Thomas Susan T Weintraub Magnus Palmblad Source Type: research

Analysis of Microbiomes from Ultra-Low Biomass Surfaces Using Novel Surface Sampling and Nanopore Sequencing
J Biomol Tech. 2023 Aug 10;34(3):3fc1f5fe.bac4a5b3. doi: 10.7171/3fc1f5fe.bac4a5b3. eCollection 2023 Sep 30.ABSTRACTThe rapid assessment of microbiomes from ultra-low biomass environments such as cleanrooms or hospital operating rooms has a number of applications for human health and spacecraft manufacturing. Current techniques often employ lengthy protocols using short-read DNA sequencing technology to analyze amplified DNA and have the disadvantage of a longer analysis time and lack of portability. Here, we demonstrate a rapid (~24 hours) on-site nanopore-based sequencing approach to characterize the microbiome of a NASA...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Anna C Simpson Scott Tighe Season Wong Patrick Leo Ceth Parker Atul M Chander Michael Williams Hao-Wei Wu Kasthuri Venkateswaran Nitin K Singh Source Type: research

Article Watch: September, 2023
J Biomol Tech. 2023 Sep 25;34(3):3fc1f5fe.bdb707e7. doi: 10.7171/3fc1f5fe.bdb707e7. eCollection 2023 Sep 30.ABSTRACTThis column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, AU-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Tel; (706) 713-2216: Fax; (706) 713-2221: Email; cslaught@uga.edu or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the Association.PMID:37969873 | PMC:...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Clive Slaughter Source Type: research

The Association of Biomolecular Resource Facilities Proteome Informatics Research Group Study on Metaproteomics (iPRG-2020)
This study provides insights into the status of metaproteomics bioinformatics in participating laboratories and core facilities.PMID:37969874 | PMC:PMC10644979 | DOI:10.7171/3fc1f5fe.a058bad4 (Source: Journal of Biomolecular Techniques : JBT)
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Pratik D Jagtap Michael R Hoopmann Benjamin A Neely Antony Harvey Lukas K äll Yasset Perez-Riverol Milky K Abajorga Julie A Thomas Susan T Weintraub Magnus Palmblad Source Type: research

Analysis of Microbiomes from Ultra-Low Biomass Surfaces Using Novel Surface Sampling and Nanopore Sequencing
J Biomol Tech. 2023 Aug 10;34(3):3fc1f5fe.bac4a5b3. doi: 10.7171/3fc1f5fe.bac4a5b3. eCollection 2023 Sep 30.ABSTRACTThe rapid assessment of microbiomes from ultra-low biomass environments such as cleanrooms or hospital operating rooms has a number of applications for human health and spacecraft manufacturing. Current techniques often employ lengthy protocols using short-read DNA sequencing technology to analyze amplified DNA and have the disadvantage of a longer analysis time and lack of portability. Here, we demonstrate a rapid (~24 hours) on-site nanopore-based sequencing approach to characterize the microbiome of a NASA...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Anna C Simpson Scott Tighe Season Wong Patrick Leo Ceth Parker Atul M Chander Michael Williams Hao-Wei Wu Kasthuri Venkateswaran Nitin K Singh Source Type: research

Article Watch: September, 2023
J Biomol Tech. 2023 Sep 25;34(3):3fc1f5fe.bdb707e7. doi: 10.7171/3fc1f5fe.bdb707e7. eCollection 2023 Sep 30.ABSTRACTThis column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, AU-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Tel; (706) 713-2216: Fax; (706) 713-2221: Email; cslaught@uga.edu or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the Association.PMID:37969873 | PMC:...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Clive Slaughter Source Type: research

The Association of Biomolecular Resource Facilities Proteome Informatics Research Group Study on Metaproteomics (iPRG-2020)
This study provides insights into the status of metaproteomics bioinformatics in participating laboratories and core facilities.PMID:37969874 | PMC:PMC10644979 | DOI:10.7171/3fc1f5fe.a058bad4 (Source: Journal of Biomolecular Techniques : JBT)
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Pratik D Jagtap Michael R Hoopmann Benjamin A Neely Antony Harvey Lukas K äll Yasset Perez-Riverol Milky K Abajorga Julie A Thomas Susan T Weintraub Magnus Palmblad Source Type: research

Analysis of Microbiomes from Ultra-Low Biomass Surfaces Using Novel Surface Sampling and Nanopore Sequencing
J Biomol Tech. 2023 Aug 10;34(3):3fc1f5fe.bac4a5b3. doi: 10.7171/3fc1f5fe.bac4a5b3. eCollection 2023 Sep 30.ABSTRACTThe rapid assessment of microbiomes from ultra-low biomass environments such as cleanrooms or hospital operating rooms has a number of applications for human health and spacecraft manufacturing. Current techniques often employ lengthy protocols using short-read DNA sequencing technology to analyze amplified DNA and have the disadvantage of a longer analysis time and lack of portability. Here, we demonstrate a rapid (~24 hours) on-site nanopore-based sequencing approach to characterize the microbiome of a NASA...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Anna C Simpson Scott Tighe Season Wong Patrick Leo Ceth Parker Atul M Chander Michael Williams Hao-Wei Wu Kasthuri Venkateswaran Nitin K Singh Source Type: research

Article Watch: September, 2023
J Biomol Tech. 2023 Sep 25;34(3):3fc1f5fe.bdb707e7. doi: 10.7171/3fc1f5fe.bdb707e7. eCollection 2023 Sep 30.ABSTRACTThis column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, AU-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Tel; (706) 713-2216: Fax; (706) 713-2221: Email; cslaught@uga.edu or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the Association.PMID:37969873 | PMC:...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Clive Slaughter Source Type: research

The Association of Biomolecular Resource Facilities Proteome Informatics Research Group Study on Metaproteomics (iPRG-2020)
This study provides insights into the status of metaproteomics bioinformatics in participating laboratories and core facilities.PMID:37969874 | PMC:PMC10644979 | DOI:10.7171/3fc1f5fe.a058bad4 (Source: Journal of Biomolecular Techniques : JBT)
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Pratik D Jagtap Michael R Hoopmann Benjamin A Neely Antony Harvey Lukas K äll Yasset Perez-Riverol Milky K Abajorga Julie A Thomas Susan T Weintraub Magnus Palmblad Source Type: research

Analysis of Microbiomes from Ultra-Low Biomass Surfaces Using Novel Surface Sampling and Nanopore Sequencing
J Biomol Tech. 2023 Aug 10;34(3):3fc1f5fe.bac4a5b3. doi: 10.7171/3fc1f5fe.bac4a5b3. eCollection 2023 Sep 30.ABSTRACTThe rapid assessment of microbiomes from ultra-low biomass environments such as cleanrooms or hospital operating rooms has a number of applications for human health and spacecraft manufacturing. Current techniques often employ lengthy protocols using short-read DNA sequencing technology to analyze amplified DNA and have the disadvantage of a longer analysis time and lack of portability. Here, we demonstrate a rapid (~24 hours) on-site nanopore-based sequencing approach to characterize the microbiome of a NASA...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Anna C Simpson Scott Tighe Season Wong Patrick Leo Ceth Parker Atul M Chander Michael Williams Hao-Wei Wu Kasthuri Venkateswaran Nitin K Singh Source Type: research

Article Watch: September, 2023
J Biomol Tech. 2023 Sep 25;34(3):3fc1f5fe.bdb707e7. doi: 10.7171/3fc1f5fe.bdb707e7. eCollection 2023 Sep 30.ABSTRACTThis column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive Slaughter, AU-UGA Medical Partnership, 1425 Prince Avenue, Athens GA 30606. Tel; (706) 713-2216: Fax; (706) 713-2221: Email; cslaught@uga.edu or to any member of the editorial board. Article summaries reflect the reviewer's opinions and not necessarily those of the Association.PMID:37969873 | PMC:...
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Clive Slaughter Source Type: research

The Association of Biomolecular Resource Facilities Proteome Informatics Research Group Study on Metaproteomics (iPRG-2020)
This study provides insights into the status of metaproteomics bioinformatics in participating laboratories and core facilities.PMID:37969874 | PMC:PMC10644979 | DOI:10.7171/3fc1f5fe.a058bad4 (Source: Journal of Biomolecular Techniques : JBT)
Source: Journal of Biomolecular Techniques : JBT - November 16, 2023 Category: Molecular Biology Authors: Pratik D Jagtap Michael R Hoopmann Benjamin A Neely Antony Harvey Lukas K äll Yasset Perez-Riverol Milky K Abajorga Julie A Thomas Susan T Weintraub Magnus Palmblad Source Type: research