Medication adherence among Albanian patients with rheumatoid arthritis.
Authors: Këlliçi S, Mihani J, Miraçi M, Fida M, Backa T Abstract Non-adherence in patients with rheumatoid arthritis (RA) using disease-modifying antirheumatic drugs (DMARDs) may lead to increased disease activity and symptoms, poorer quality of life and increased costs related to the disease treatment. The aim of this study is to determine the level of adherence to the treatment protocols of a chronic disease such as rheumatoid arthritis in the Albanian population and to investigate the predictors of adherence to medication. This is a single-center cross-sectional study in which included 125 pat...
Source: Journal of Biological Regulators and Homeostatic Agents - January 19, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Effect of cholecalciferol on cd3+cd25+ T cells in patients with severe sepsis.
Authors: Li GH, Ren DH, Yu S, Chen J, Fang K, Li ZH, Cai L, Shi ZL, Zhang JY, Ma JI Abstract Non-adherence in patients with rheumatoid arthritis (RA) using disease-modifying antirheumatic drugs (DMARDs) may lead to increased disease activity and symptoms, poorer quality of life and increased costs related to the disease treatment. The aim of this study is to determine the level of adherence to the treatment protocols of a chronic disease such as rheumatoid arthritis in the Albanian population and to investigate the predictors of adherence to medication. This is a single-center cross-sectional study in which include...
Source: Journal of Biological Regulators and Homeostatic Agents - January 19, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Nerve growth factor administration on cultured human ligamentocytes: an in vitro pilot study.
This study analyzed the effect of 200 ng/ml on cultured human ligamentocytes by evaluating cell proliferation, cell phenotype and gene expression. The MTT test excluded an influence on cell viability at 7 and 14 days. Regarding cell phenotype, we observed that NGF might promote the synthesis of COL1A1. On the other hand, real time PCR showed that NGF did not influence gene expression of COL3A1, FGF-BETA, IGF1, MMP2, MMP3, MMP9 and MMP13. However, COL1A1 gene was significantly upregulated in treated cell at 14 days. Our results suggest that NGF may have an anabolic effect on ligament. Additional investigations are necessary...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Multi-omics analysis of synovial fluid: a promising approach in the study of osteoarthritis.
Authors: Vicenti G, Bizzoca D, Carrozzo M, Solarino G, Moretti B Abstract Osteoarthritis (OA), affecting 250 million individuals worldwide, is a significant social health problem. Therefore, the search for synovial fluid (SF) biomarkers that could anticipate the diagnosis of OA is gaining increasing importance in orthopaedics. This review summarizes the recent progresses preformed in the multi-omics approach to OA, mainly focusing on proteome and metabolome analysis of SF. Proteomics of the SF has shown the up-regulation of several components of the classic complement pathway in OA samples, including C1, C2, C3, C4...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

New insights into the treatment of non-healing diabetic foot ulcers.
Authors: Vicenti G, Bizzoca D, Caruso I, Nappi VS, Giancaspro G, Carrozzo M, Moretti B Abstract Diabetic foot ulcers (DFUs) are one of the most serious and devastating complication of diabetes mellitus, affecting about 15% of diabetic patients. This review describes the innovative treatment options currently available in the treatment of non-healing DFUs. The use of Platelet-Rich-Plasma (PRP) is a safe and valid approach in the treatment of DFUs. However, the methods used to obtain and prepare autologous PRP vary between the studies, thus further evidences are eagerly awaited. Adipose tissue-derived mesenchymal ste...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Biophysical stimulation of the knee with PEMFs: from bench to bedside.
Authors: Vicenti G, Bizzoca D, Nappi VS, Moretti F, Carrozzo M, Belviso V, Moretti B Abstract Clinical biophysics investigates the relationship between non-ionizing physical energy and the human body. Although several types of electrical stimulation devices have received US FDA approval for orthopaedic application, the use of Pulsed Electromagnetic Field (PEMFs) play a central role in joint biophysics. This narrative review aims to summarize the current evidences on the efficacy of PEMF-therapy in the treatment of knee articular diseases. Preclinical studies have assessed the effects of PEMFs on chondrocytes, synov...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Tantalum monoblock cups in total hip arthroplasty: clinical results and outcome predictors.
This study aims to report the minimum 2-year follow-up results of the tantalum monoblock cup in primary THA and to identify possible outcome predictors. Eighty-eight porous tantalum monoblock acetabular cup in primary THA were reviewed. The Harris Hip Score (HHS) and the Short Form-36 Health Survey (SF-36) were used for the evaluation of outcomes. Radiographic evaluation included acetabular component orientation, presence of bone gaps, radiolucent lines, new bone formation and heterotopic ossifications. After a mean follow-up of 55.4±19.5 months, no component revision was noted. The HHS improved from 43.6±14....
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

How cartilage status can be related to joint loads in anterior cruciate ligament reconstruction: a preliminary analysis including MRI t2 mapping and joint biomechanics.
Authors: Cassiolas G, Berni M, Marchiori G, Bontempi M, Bragonzoni L, Valente G, Grassi A, Zaffagnini S, Visani A, Lopomo NF Abstract The knee is the largest and most complex joint in the human body. Traumatic events, such as anterior cruciate ligament (ACL) tear, can lead to an alteration of joint tissues homeostasis. Literature reports an evident correlation between abnormal joint biomechanics and the status of articular tissues. These alterations, due to a sub-optimal ACL reconstruction, may result in an increasing risk of developing degenerative pathologies, such as osteoarthritis. Thus, the identification of t...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Biomaterials and biophysical stimuli for bone regeneration.
Authors: Fassina L, Bloise N, Montagna G, Visai L, Mognaschi ME, Benazzo F, Magenes G Abstract First, we review basic concepts of Tissue Engineering, that is, how the tensegrity is able to modulate the cell behavior. Then, we review our experimental results regarding the bone tissue engineering via biomaterials and bioreactors. PMID: 30644280 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Single dose cefazolin is safe and effective for pre-operative prophylaxis in orthopaedic oncology.
Authors: Ziranu A, Lillo M, Fantoni M, Maffulli N, Maccauro G Abstract Surgical site infections (SSI) are a common potentially preventable complication after surgical procedures. A standardized antibiotic prophylaxis in elective orthopaedic surgery plays a major role in lowering SSI. At present, there is little published evidence regarding standardized antibiotic prophylaxis in orthopaedic oncological surgery. We introduced a prophylactic antibiotic protocol for orthopaedic oncological surgery in our hospital. The proposed protocol consists in "one-shot" intravenous administration of Cefazolin 2g, 30 min ...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Biomarkers and infections in orthopedics: our experience and literature review.
Authors: Di Benedetto P, Buttironi MM, Causero A Abstract The issue of infections in Orthopedics is rising in importance day by day. In management of Periprosthetic Joint Infection (PJI), classical biomarkers involved in diagnosis process are C-reactive protein (CRP) and Erythrocyte Serum Rate (ESR). Although wide use in PJI diagnosis process, CRP and ESR are not sensitive and specific enough for a correct diagnosis. Orthopedic surgeons, Microbiologists and Infectivologists are very interested in research about new (or rediscovered) biomarkers that can help in the diagnosis of orthopedics infections. In our Institu...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

A local anesthesia without tourniquet for distal fibula hardware removal after open reduction and internal fixation: the safe use of epinephrine in the foot. A randomized clinical study.
In conclusion, the WALANT can be considered as a suitable option for distal fibula hardware removal in selected patients; it shows important clinical and economic advantages compared to the traditional loco-regional anaesthesia with tourniquet. This study also lays the foundation for the use of the WALANT beyond the field of hand surgery only. PMID: 30644283 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Cryotherapy efficacy and safety as local therapy in surgical treatment of musculoskeletal tumours. A retrospective case series of 143 patients.
Authors: Colangeli S, Parchi P, Andreani L, Beltrami G, Scoccianti G, Sacchetti F, Ceccoli M, Totti F, Campanacci DA, Capanna R Abstract Cryotherapy, also called Cryoablation (CA), is a technique that provides a local treatment to various pathological conditions. In Musculoskeletal tumours management, Cryoablation is well accepted and validated as a treatment in palliative cures for metastatic patients. Recently, CA has been proposed also as an alternative to radiofrequency ablation in osteoid osteoma and other benign tumour treatment with promising results. Cryotherapy with argon ice-balls as local adjuvant in ope...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Polymethylmethacrylate-augmented fenestreted pedicle-screw fixation in low bone quality patients: a case series and literature review.
Authors: Ghermandi R, Pipola V, Colangeli S, Parchi P, Andreani L, Capanna R, Gasbarrini A Abstract The use of pedicle screws in low bone quality patients implicates risks of secondary implant loosening for grip lack. In fact, the result is a reduced mechanical stability at bone-screw interface and consequently an increased chance of pullout and hardware failure. Augmentation techniques have been described for many years and fenestrated screws that allow cement injection is one of them. This is a retrospective observational study of patients treated at our department with polymethylmethacrylate- (PMMA) augmented fe...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

A rare case of Hoffa's fat pad herniation in a young patient: dynamic ultrasound and MRI diagnosis.
We presented a rare case of Hoffa's fat pad herniation through the joint capsule, in a young child without history of knee injuries that has never been described before in the literature. PMID: 30644286 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Musculoskeletal hydatid disease: report of a rare case with primary and exclusive localization in right hemi-pelvis and femur.
We present a rare case of male patient of 42 year-old with diagnosis of HD with primary and exclusive localization in right hemi-pelvis and femur. PMID: 30644287 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Comparison of early functional outcomes between two different surgical approaches for total hip arthroplasty.
Authors: Sirtori P, Peretti GM, Ulivi M, Ciliberto R, Lombardo MD, Verdoni F, Michielon G, Mangiavini L Abstract Total Hip Arthroplasty (THA) is considered the most successful treatment for advanced hip osteoarthritis. Different surgical approaches for THA are available and they have shown excellent outcomes in the long-term follow-ups. However, few studies have analyzed the functional outcomes in the first days after a THA surgery. The purpose of this study was to compare the early functional outcomes between two different surgical techniques: a minimally invasive direct anterior approach (mini-DAA) and a postero-...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Characterization of different in vitro culture conditions to induce a fibro-chondrogenic differentiation of swine adipose-derived stem cells.
Authors: Tessaro I, Di Giancamillo A, Benasciutti E, Nguyen VT, Polito U, Mangiavini L, Peretti GM Abstract Meniscus repair is still a challenge for orthopaedic surgeons as this tissue has a scarce healing potential due to the limited vascularization and to the lack of progenitor cells. Lately, several tissue engineering strategies combining cells and scaffolds have been developed. Adipose-derived stem cells (ASCs) represent a novel cell source for meniscus repair as they are easy to harvest, and they possess an intrinsic chondrogenic potential. The aim of our study was to analyze and compare the chondrogenic diffe...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Animal models for cartilage repair.
Authors: Tessaro I, Nguyen VT, Di Giancamillo A, Agnoletto M, Verdoni F, Domenicucci M, Scurati R, Peretti GM, Mangiavini L Abstract Cartilage lesions still represent an unsolved problem: despite the efforts of the basic and translational research, the regeneration of this tissue is far from being reached (1-3). Articular cartilage lesions can be divided in two main groups: superficial or partial defects and full-thickness defects (4, 5). Partial lesions are not able to self-heal because multipotent cells from the bone marrow cannot reach the area leading to a progressive degeneration of the tissue (6). Conversely,...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

External fixation versus titanium endomedullary nail in the treatment of diaphyseal fractures of the lower limb in pediatric age: our experience.
Authors: Verdoni F, Curci D, Grasso F, Agnoletto M, Scurati R, Peretti GM Abstract In the past, the use of external fixation technique (FE) in children was limited, as the patient's compliance to the treatment was scarce due to an important visual impact for the little patient. With the evolution of the surgical approach and the technology of fixators, we have been able to considerably implement the treatment possibilities, thus allowing for an immediate load bearing of the operated limb and for the early mobilization of the joints. The FE technique does not represent an overcoming of classical synthesis techniques...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Medical management of acute non-specific low back pain: comparison of different medical treatments, one center's retrospective analysis.
Authors: Perna A, Ricciardi L, Barone G, Tamburrelli FC, Proietti L, Pola E Abstract Within the developed countries, low back pain and related ailments represent one of the most relevant conditions, affecting both health and socio-economic systems. Non-specific acute low back pain (nsALBP) could represent either an isolated event or the presenting symptom of an underlying condition. Its management is usually medical and radiological investigations should be avoided. However, a general agreement on the best pharmacological treatment is still missing. In the present study, we retrospectively collected patients' data ...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Reinfusion-drains reduce blood transfusions in total joint arthroplasty.
This study aims to evaluate the effect of postoperative blood recovery with reinfusion drains on hematologic parameters and blood transfusion rate in patients undergoing total joint arthroplasty. Three-hundred-and-forty-four patient records were reviewed and 271 patients were included in the study; 56.8% of patients were treated with postoperative cell salvage procedure using reinfusion drains (PCS) and 43.2% had closed-suction drain (CSD) postoperatively. In comparison to the CSD group, the PCS group showed higher hemoglobin (Hb) levels on the first and second days postoperatively but no statistical differences were noted...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Aggressive hemangioma starting from l5 body, treated with mass embolization and radiation therapy with protons: a case report.
We present a clinical case report of a young woman, affected by aggressive L5 hemangioma causing a spinal canal stenosis with associated sciatalgic symptoms. We performed a review of the current literature on the treatment options, giving the rationale of our treatment choice (mass embolization and radiation therapy with protons). PMID: 30644294 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Primary total knee replacement in hemophiliacs: experience of a single institution over fourteen years of surgical procedures.
According to this study, there are three main factors that can influence long-term and early surgical outcomes: post-operative fibrosis, a previous synovectomy and presence of inhibitors. Even if our results are slightly suboptimal compared to those obtained in non-hemophilic patients, this study shows that TKR is an effective surgical procedure in hemophiliacs. PMID: 30644295 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Secondary femur fracture following treatment with anterograde nailing: the state of the art.
Authors: Cazzato G, Masci G, Liuzza F, Capasso L, Florio M, Perisano C, Vitiello R, Ciolli G, Maccauro G Abstract Cephalomedullary nailing (CMN) currently represents the best surgical technique for the treatment of intertrochanteric hip fractures. Although the success of CMN in terms of functional recovery and fracture healing, in clinical practice there are many complications. Later femur fracture following treatment of trochanteric fracture with CMN is not a very frequent complication but, when it occurs, its treatment is the most complex, because of the increase of peri-operative mortality. There are studies in ...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Mueller-Weiss disease: review of the literature.
This study provides a review of the literature about the epidemiology, etio-pathogenesis, clinical, radiological findings and therapeutic alternatives. PMID: 30644297 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Transiliosacral fixation using the O-ARM2 ® and STEALTHSTATION® navigation system.
Transiliosacral fixation using the O-ARM2® and STEALTHSTATION® navigation system. J Biol Regul Homeost Agents. 2018 Nov-Dec;32(6 Suppl. 1):163-171 Authors: Liuzza F, Capasso L, Florio M, Mocini F, Masci G, Cazzato G, Ciolli G, Silluzio N, Maccauro G Abstract Pelvic fractures are quite uncommon, they represent only 2-8% of all fractures. Osteosynthesis with percutaneous trans-Iliosacral screw is recognized as one of the standard procedures for the treatment of unstable posterior pelvic ring lesions. Because of the high number of complications associated with the conservative treatment of these k...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

The biomolecular interactions between endothelium and bone cells: an overview.
Authors: Carulli C, Innocenti M, Lazzarini F, Villano M, Pasta G, Brandi ML, Innocenti M Abstract Bone turnover is a complex set of different molecules pathways and it is strictly connected with bone vasculature. It includes every bone process concerning bone modelling and remodelling such us skeletal growth and healing process. A fundamental component of this bone architecture is played by the endothelium, that acts in a paracrine fashion on other bone stromal cells via humoral factors, growth factors and chemokines/cytokines. The alteration of those biochemical interactions between endothelium, vasculature and bo...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

How to obtain better results, choice of implant or surgical technique? A new surgical technique with an extramedullary referenced cutting guide and a blended alignment method.
Authors: Rossi SMP, Ghiara M, Castelli A, Ivone A, Jannelli E, Benazzo F Abstract Measured resection and ligament balancing techniques are the most used in TKA; both require, in the majority of knee arthroplasty systems, a femoral intramedullary canal referencing for the distal femoral cut. Fat embolism, activation of coagulation and bleeding may occur from the reamed canal; this can be avoided with different solutions such as navigation, patient specific instruments or the use of an extramedullary device. The FuZionTM tensor was created as a blended method with the purpose to overcome the downsides of both techniq...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Hydatid cyst in the spine. The alliance between surgeon and infectivologist is winning: a case report.
Authors: Cuzzocrea F, Jannelli E, Ivone A, Vanelli R, Ghiara M, Fioruzzi A, Brunetti E, Benazzo F Abstract Hydatid Cyst is a protozoal disease, whose spinal localization may be cause of pain and severe inability due to neurological symptoms and instability. Treatment combines the difficulties to eradicate the cyst with the spine regional constraints. The case of a 45-year-old woman is reported, affected by hydatid cyst in the spine, submitted to combination of medical treatment and surgery (double approach gross total excision and reconstruction) and followed up for 6 years. The treatment strategy allowed an excell...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Evaluation of the use of autologous micro-fragmented adipose tissue in the treatment of knee osteoarthritis: preliminary results of a randomized controlled trial.
In this study, we analyzed the role of these cells in modifying the pathological microenvironment present in knee osteoarthritis. This is an interventional, prospective, randomized, controlled study. Starting from June 2017, 39 patients with grade III and IV knee osteoarthritis of Kellgren-Lawrence were enrolled, aged between 45 and 75 years, with pain greater than or equal to 6 according to the VAS scale, without ligament instability, with an axial deviation not greater than 10° and with a BMI between 18 and 30. The control group underwent an arthroscopic debridement, while the experimental group underwent an arthrosc...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Total hip arthroplasty in rapidly progressive osteoarthritis of the hip: clinical and radiological outcome of a consecutive series of patients.
Authors: Chompoosang T, D'Apolito R, Lacagnina C, Peretti G, Zagra L Abstract Rapidly progressive osteoarthritis (RPOA) is an uncommon subset of osteoarthritis (OA) characterized by rapid joint degeneration and destruction. The treatment of choice for these patients has traditionally been total hip arthroplasty (THA) because nonoperative treatments modalities are not effective. RPOA can lead to acetabular bone loss and consequently intraoperative technical difficulties making joint reconstruction in such patients a challenge for the surgeon. The purpose of this study was to determine the clinical and radiological r...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Treatment of Vancouver B1, C periprosthetic hip fractures with periprosthetic polyaxial locking plate system: A 3-year follow-up.
Authors: Castelli A, Rossi SMP, Rocca L, Jannelli E, Benazzo F Abstract The incidence of periprosthetic fractures after primary knee and hip arthroplasty is around 2.5% and is increasing after revision surgery up to 4%. Management of these fractures is often particularly demanding and expensive. The primary aim of the presented study is to describe our experience in using a precontoured periarticular polyaxial standard plating system in a cohort of patients with homogeneous fracture type (Vancouver B1, C), comparing our experience with other surgical solutions. In stable implants, the primary strategies aim for fra...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Targeting VEGF-A in cartilage repair and regeneration: state of the art and perspectives.
Authors: Vadalà G, Russo F, Musumeci M, Giacalone A, Papalia R, Denaro V Abstract Osteoarthritis (OA) is the most common joint disorders in western populations, and is characterized by a progressive degradation of articular cartilage (AC) leading to loss of joint function. Methods to cure, delay or prevent the onset of OA and/or improve AC repair strategies have high clinical and socioeconomic impact. Possible innovative strategies envisioned for early OA treatments or cartilage repair include the implantation/injection of mesenchymal progenitors (MPs)-based constructs or cell-free bioactive scaffolds/hydrog...
Source: Journal of Biological Regulators and Homeostatic Agents - January 17, 2019 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Stress, inflammation and natural treatments.
Authors: Theoharides TC, Kavalioti M Abstract Stress and inflammation have become the curses of our times and are the main pathogenetic factors in multiple diseases that are often comorbid and include allergies and asthma, eczema and psoriasis, fibromyalgia syndrome, mast cell activation syndrome, irritable bowel syndrome, myalgic encephalomyelitis/chronic fatigue syndrome and autism spectrum disorder (ASD). Unfortunately, there are no effective drugs. Cross-talk between mast cells and microglia in the hypothalamus and amygdala could explain stress-induced inflammation. We recently showed that the "alarmin&quo...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Does the purinergic system affect extracellular matrix functions in the central nervous system?
Authors: Zuccarini M, Carluccio M, Ziberi S, Giuliani P, Buccella S, Conti C, Ciccarelli R, Di Iorio P Abstract Exracellular matrix (ECM) consists of a plethora of proteins and polysaccharides, which aggregate into an organized network connected to the surface of the producing cells. It is structurally and functionally present in all components of tissues and organs and represents the substrate on which cells adhere, migrate, proliferate and differentiate, influencing their survival, shape and function. In response to acute (trauma) or chronic (degenerative) insults, brain ECM modifies its composition and function,...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Stimulated mast cells release inflammatory cytokines: potential suppression and therapeutical aspects.
Authors: Varvara G, Tettamanti L, Gallenga CE, Caraffa A, D'Ovidio C, Mastrangelo F, Ronconi G, Kritas SK, Conti P Abstract Mast cells (MCs) are derived from bone marrow precursors and are immune cells involved in acute and chronic inflammation. MCs are ubiquitous and play a crucial role in innate and acquired immunity. They are activated through cross-linking of their surface high affinity receptors (FcεRI), leading to immediate secretion of stored inflammatory mediators, and late production and release of pro-inflammatory cytokines/chemokines without degranulation. Therefore, MCs are important in inflamma...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Cyclin-dependent kinase 7 is a potential therapeutic target in papillary thyroid carcinoma.
In conclusion, our study might provide a novel potential target for PTC therapy. PMID: 30574740 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

17 β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis.
17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis. J Biol Regul Homeost Agents. 2018 Nov-Dec;32(6):1369-1377 Authors: Panic A, Stanimirovic J, Obradovic M, Zafirovic S, Sudar-Milovanovic E, Petrovic N, Isenovic ER Abstract 17β-Estradiol (E2) is known to negatively regulate inducible nitric oxide (NO) synthase (iNOS) expression via estrogen receptor alpha (ER-α) activation in aortic vascular smooth muscle cells.Therefore, we sought to determine whether E2 can inhibit iNOS in vivo in hepatic tissue via the acti...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Role and influence of p75NTR receptor on antioxidative damage of retinal pigment epithelial cells.
In conclusion, p75 NTR receptor may be a cause of oxidative damage in RPE cells. . PMID: 30574742 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Apoptosis of hepatocarcinoma cells Hepg2 induced by Huaier extract through regulation of HBx and CEACAM1 gene expression..
Authors: Zhong LH, Zhu LY, Zhao YY, Wang W, Lu BL, Wang Y, Cheng Y, Ma YJ Abstract Huaier can effectively inhibit the growth of tumor cells by enhancing the immune system. However, the mechanism of its function is still not clear. The current study aimed to explore the possible mechanism of Huaier in inhibiting human hepatocarcinoma cells by observing its effect on proliferation and invasion in hepatocarcinoma cells, HepG2 and HepG2-X, which stably express the HBx gene, and by comparing the levels of mRNA transcription and protein expression of HBx and CEACAM1 in HepG2 cells and HepG2-X cells when treated with diff...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

SOX2 gene expression and its role in triple negative breast cancer tissues.
Authors: Yao GD, Niu YY, Chen KX, Meng HX, Yao GD, Song HT, Tian ZN, Geng JS, Feng MY Abstract The aim of this work was to study the expression of SOX2 gene in triple negative breast cancer and its role. One hundred and twenty specimens of paraffin-embedded triple negative breast cancer (TNBC) tissues were collected from Harbin Medical University Cancer Hospital, Heilongjiang, China between January 2014 and March 2018. The expression of SOX2 was detected using immunohistochemistry, and the relationship between the expression of SOX2 and clinical features was analyzed. Breast cancer cell lines (normal group, SOX2 in...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Longitudinal study on the effect of oral hygiene measures on the salivary count of microbial species with cariogenic potential.
Authors: Esin S, Pasini M, Miceli M, Cosseddu G, Giuca MR, Batoni G Abstract The effect of oral hygiene education measures and professional tooth cleaning on the salivary levels of microbial species with high cariogenic potential (i.e. Streptococcus mutans, Lactobacillus spp. and Candida albicans) was evaluated at different time points. At time 0, high salivary carriage rates were recorded in the study group (n=30). Fifty percent of the subjects harbored all three species in their saliva, 27% harbored 2 species, and 23% only one species. At 3 months after oral hygiene measures, a statistically significant reduction...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Impact of treatments on fecal microbiota and fecal metabolome in symptomatic uncomplicated diverticular disease of the colon: a pilot study.
Authors: Laghi L, Mastromarino P, Elisei W, Capobianco D, Zhu CL, Picchio M, Giorgetti G, Brandimarte G, Tursi A Abstract Symptomatic uncomplicated diverticular disease (SUDD) affects 50% of people having diverticulosis. We performed a pilot study assessing the effect of current treatments on fecal microbiota and metabolome in SUDD. Thirteen consecutive females with SUDD were treated with a 2-week therapeutic trial of 30 g/day fiber supplementation (3 patients), 1.6 g/day of mesalazine (3 patients), 900 billion/day of probiotic mixture VivoMixx® (3 patients), or 800 mg/day of rifaximin (4 patients). Stool sampl...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

One-year follow-up showing effects of single intra-articular injection of hyaluronic acid (1,500-2,000 kDa) in symptomatic knee osteoarthritis.
This study assesses the duration of effectiveness of a single intra-articular hyaluronic acid injection in a large population of patients with knee osteoarthritis. This retrospective post-marketing cohort study collected data from the ANTIAGE Registry (http://www.antiagefbf.it/registro), selecting patients of age ≥ 40 years, with symptomatic knee osteoarthritis (Kellgren-Lawrence grade I-III) of ≥ 12 months duration, and ≥12 months of follow-up. Patients had received a single intra-articular injection of high molecular weight hyaluronic acid (1,500-2,000 kDa) at baseline. WOMAC Osteoarthritis Index total scores me...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Molecular mechanism of action of valproate acid alone or in combination with chlorpromazine in the epigenetic regulation of schizophrenia.
This study aimed to assess the molecular mechanism of the histone deacetylase inhibitor (HDACI) valproate acid (VPA) alone or in combination with the antipsychotic drug chlorpromazine in the epigenetic regulation of schizophrenia. A total of 60 perinatal CD-SD rats were divided in a control group (16 animals) and a schizophrenia model group (44 animals). For the schizophrenia model group the rats received phencyclidine (PCP) 10 mg/kg/day by intradermal injection on days 7, 9, and 11 after birth. The model was confirmed by the Morris water test in 40 rats. The control and model rats were divided into 7 groups. The Real Time...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Tanshinone IIA protects against cardiac fibrosis through inhibition of β-tubulin expression.
Tanshinone IIA protects against cardiac fibrosis through inhibition of β-tubulin expression. J Biol Regul Homeost Agents. 2018 Nov-Dec;32(6):1451-1455 Authors: Zhang Y, Zhang S, Chen X Abstract Cardiac fibrosis is a significant global health problem that occurs after pathological stimuli to the cardiovascular system. Previous researchers have shown the protective role of Salvia miltiorrhiza Bunge (Danshen) as well as its extracted compound Tanshinone IIA (Tan IIA) against cardiac fibrosis. However no previous work has shown the effects of Tanshinone IIA on cardiac cytoskeleton or the possible rela...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Brassinosteroid biosynthesis, stress resistance in plants, and application of brassinosteroids in plant biotechnology.
Authors: Ali MM, Zhang WP, Ihsan MA, Zafar H, Rauf QA, Akhtar MK Abstract Brassinosteroids (BRs) are newly discovered plant hormones that protect the plants from biotic and abiotic stress. Plants produce these hormones at all times, however, the quantity and location of their production vary. It has been demonstrated that BRs help the plants to regulate their response to stress conditions and make them more resistant to pest attack, extreme hot or cold environment, water scarcity, and salinity, among other types of stress. Manipulation of genes involved in the synthesis of BRs in different plants is a feasible stra...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Correlation between rheumatoid arthritis and immunological changes in a rheumatoid arthritis rat model.
This study aimed to investigate the characteristics of the immunological profile of the cells of the synovial membrane and the expression of IL-10 and IL-17 in a rheumatoid arthritis rat model in order to provide a targetdirected treatment for immunological control. Eighty female Wistar rats were randomly divided into a rheumatic arthritis model group (model group) and a control group, 40 animals per group. After the successful rheumatoid arthritis rat model was obtained, 10 animals were sacrificed from each group every week starting from the third week till the sixth week and the expression levels of CD3, CD21, and CD68 i...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

miR-200b promotes cell proliferation and invasion in t-cell acute Lymphoblastic leukemia through NOTCH1.
In this study, we evaluated the impact of miR-200b in T-ALL cell proliferation, survival and invasion using gain and loss of function approaches. Human Jurkat cells, a widely used in-vitro T-ALL cell model, were transfected with miR-200b mimic or miR-200b inhibitor. miR-200b mimics substantially inhibited Jurkat cell proliferation and invasion while significantly stimulating cell apoptosis compared to the control miRNA-treated cells. In contrast, Jurkat cells treated with anti-miR200 demonstrated induction of cell growth and invasion but repression of cell apoptosis. Such effect was accompanied by the corresponding alterat...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2018 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research