Clinical effects of thermotherapy in combination with intracavitary infusion of traditional Chinese medicine in the treatment of malignant pleural effusion.
Authors: Gu Y, Jiang L, Miao JH, Liang TS, Kan QC, Yang DK Abstract Malignant fluid, a commonly seen tumor associated complication, mainly includes peritoneal effusion, malignant pleural effusion and pericardial effusion. It can produce huge negative influence on the quality of life of patients and even lead to death. Treatment of malignant effusion is one of the effective measures for improving life expectancy of patients. To evaluate the effect of thermotherapy in combination with intracavitary infusion of Kangai injection in treating malignant pleural effusion, 195 patients who received treatment from April 2010...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Effects of carbomer eye drops in combination with orthokeratology lens in treating adolescent myopia.
Authors: Shi YH, Zhou LT, Zhang CX, Li YZ, Zhang JZ, Zhou HM, Li YG, Liu T, Zhang LL, Sun LN, Chen Z Abstract To investigate the effects of carbomer eye drops (CED) during long-time wearing of overnight orthokeratology lens of adolescents with myopia, 260 teenagers with myopia treated in the Henan Provincial People’s Hospital from June 2012 to August 2014 and followed-up for more than 2 years were enrolled. All the patients underwent regular fitting of orthokeratology lens. They were divided into a CED (Vidisic) group (130 cases, 260 eyes treated with CED) and rewetting drops (RD) (Baushe and Lomb) group (130 case...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Acupuncture in endocrine disorders: a critical appraisal.
Authors: Deng YZ, Li LB, Xu LG, Zhou D, Wei LJ, Liu Y Abstract Acupuncture is an integral part of ancient Chinese medical practice. The technique has been used extensively in pain relief and is being tried for many other chronic conditions. Industrial development and affluence lead to the increase in the prevalence of many endocrine disorders such as diabetes, obesity, and polycystic ovarian disease. The rising prevalence of the endocrine morbidity is observed in both the developing and developed nations. The management of these disorders involves major lifestyle modification coupled with a long-term drug intake. I...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Mechanism of serum miR-21 in the pathogenesis of familial and triple negative breast cancer.
Authors: Yang L, Feng Y, Qi P, Xu S, Zhou Y Abstract The aim of this study was to clarify the mechanism of miR-21 in familial and triple-negative breast cancer (TNBC) by exploring the expression of serum miR-21. The sera were collected from healthy women at high risk of breast cancer. miR-39 was employed as the external reference, and real-time fluorescence quantitative PCR was used to detect the expression of serum miR-21 in 77 subjects. The miR-21 expression of the familial breast cancer group, TNBC group, and breast cancer high risk group were significantly higher than those in the normal control group and other...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Cell-based therapies for experimental diabetic nephropathy: a systematic review and meta-analysis.
In conclusion, cell-based therapies significantly improve kidney function, cardiovascular risk factors, and body factors in the treatment of DN. PMID: 28078852 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Oxidative stress in asthma: a distinct clinical and pathologic feature?
Authors: Li Y, Li GP Abstract Asthma is a type of chronic airway inflammation. Corticosteroids are inadequate for asthma therapy. However, it remains unclear whether oxidative stress is a distinct clinical and pathologic feature in asthma. We reviewed the articles on asthma-associated oxidative stress. The exposures to airborne allergens, such as house dust mite (HDM) and birch pollen, may not only trigger innate and adaptive immune responses but also cause oxidative stress damage in the airways. Allergen-induced reactive oxygen species (ROS) is involved in p38 MAPK, phosphoinositide-3-kinase (PI3K)/Akt and nuclear...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Changes of expression of estrogen and progestrone receptors, human epithelial growth factor receptor 2 and Ki-67 after neoadjuvant chemotherapy in the treatment of breast cancer.
Authors: Li ML, Dong Y, Luan SL, Zhao ZH, Ning FL Abstract Recent studies suggest that the development and prognosis of breast cancer is in close correlation to molecular subtype of breast cancer. Neoadjuvant chemotherapy has been extensively applied in the treatment of local breast cancer in advanced stage. In order to verify the correlation between expression changes of estrogen receptor, progestrone receptor, human epithelial growth factor receptor 2 and Ki-67 after neoadjuvant chemotherapy and neoadjuvant chemotherapy, we studied 120 patients with stage IIAIIIC breast cancer who underwent neoadjuvant chemothera...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

The impact of electromagnetic field at a frequency of 50 Hz and a magnetic induction of 2.5 mT on viability of pineal cells in vitro.
Authors: Koziorowska A, Pasiud E, Fila M, Romerowicz-Misielak M Abstract The impact of electromagnetic fields (EMF) on the pineal gland has been described in numerous studies, but many questions still remain unanswered. The aim of the experiment described in this study was to evaluate the effect of EMF on the viability of the pineal gland cells of pig in vitro. Primary culture of the pineal gland cells has been exposed to the influence of an EMF at a frequency of 50 Hz with 1, 2 or 3 hours and for 3 hours every 2 or 3 days. After the experiment, viability of cells was assessed by MTT assay and compared to a control...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Decreased immunoreactivity of visfatin in the pancreas and liver of rats with renovascular hypertension.
The objective of this study was to evaluate the immunoreactivity of visfatin in pancreas and liver of “two kidney, one clip” (2K1C) renovascular hypertension model in rats. The studies were carried out on the pancreas and liver of rats. After a 6-week period of the renal artery clipping procedure, 2K1C rats developed a stable hypertension. Paraffin sections were stained with hematoxylin and eosin (for general histological examination) and processed for immunolocalization of visfatin. The intensity of immunohistochemical reaction was measured using Nikon NIS-Elements Advanced Research software. The hypertension significan...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Effects of sevoflurane on cardiopulmonary function in patients undergoing coronary artery bypass.
In conclusion, although LVEF was not improved in the sevoflurane group, sevoflurane may contribute to stabilizing the cardiopulmonary function and preventing from myocardial injury. PMID: 28078857 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Use of Lactated Ringer ’s solution does not eliminate the risk of strong ion difference-related metabolic acidosis following on-pump cardiac surgery.
In conclusion, our data confirm that there is a direct correlation between a strong ion difference and base excess following on-pump cardiac surgery. The use of RL prevented significant hyperchloraemia, but did not eliminate the risk of strong ion difference-related metabolic acidosis. The change in the strong ion difference was primarily linked to perioperative changes in the serum sodium concentration. PMID: 28078858 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Role of vitacamphore in improving central pro-inflammatory cytokines following transient global ischemia.
In conclusion, VCP is likely to play a beneficial role in modulating transient global ischemia induced by CA via PIC signal mechanisms. PMID: 28078859 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Effects of two different anesthetic methods on cellular immunity of patients after liver cancer resection.
This study aims to investigate the effects of epidural block in combination with general anesthesia and general anesthesia alone on the immune function of patients undergoing primary liver cancer resection. Twenty-four patients with liver cancer who received treatment in the Third Hospital of Harbin Medical University, Heilongjiang, China, were enrolled and randomly allocated into group A and group B, with 12 in each group. The data on the T lymphocyte subpopulation, pro-inflammatory cytokines and antiinflammatory cytokines were recorded before, immediately after and 24 h after liver cancer resection to compare differences...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Interleukin-6 and interleukin-8 in diagnosing neonatal septicemia.
Authors: Wu YQ, Shen J, Zhou QL, Zhao HW, Liu LR, Liu X Abstract Neonatal septicemia (NS) is a common cause of death of newborn infants, hence early diagnosis and treatment are of the utmost importance. However, lack of specific clinical symptoms and late detection delay a correct diagnosis. It is therefore of great importance to establish auxiliary indexes for the early diagnosis of NS. To evaluate the value of interleukin (IL-6 and IL-8) in the diagnosis of NS, a prospective study was carried out. Seventy-five newborns who developed septicemia and received treatment in our hospital from January 2013 to December 2...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

A placebo-controlled trial of a proprietary lipid-lowering nutraceutical supplement in the management of dyslipidemia.
Authors: Houston M, Rountree R, Lamb J, Phipps S, Meng S, Zhang B Abstract There is an ever growing emergence in the popularity of patient-driven care. As this health and wellness model grows, inquiries into diet, lifestyle, and supplemental approaches will continue to become a focal point for the healthcare consumer. Because of this, the aim of this study is to determine the tolerability, and overall effectiveness of a proprietary multi-ingredient lipid-lowering supplement in subjects with dyslipidemia. Forty participants were recruited for a single-center, double-blind randomized, placebocontrolled trial. Study p...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Nasal irrigation with Nasir ® in children: a preliminary experience on nasal cytology.
In conclusion, this pilot study reports that nasal irrigation with Nasir® might be useful to attenuate upper airway inflammation. PMID: 28078863 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Human herpesviruses-6 and -7 encephalitis in immunocompetent infants: are they really so uncommon?
In this report, we offer evidence that HHV-6 and -7 primary infections can cause several clinical manifestations, such as encephalitis, also in immunocompetent hosts. In our experience, children with neurological symptoms suggestive of viral encephalitis should be fully investigated for these two viruses. PMID: 28078864 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Parasympathetic nervous system involvement in food allergy: description of a paediatric case.
Authors: Vitaliti G, Pavone P, Giugno GR, Vecchio M, Cocuzza S, Serra A, Maiolino L, Di Mauro P, Lubrano R, Falsaperla R Abstract The latest research data emphasize the interaction between the nervous and the immune systems. It has been demonstrated that the central nervous system (CNS) can be involved secondarily due to blood brain barrier (BBB) disruption via pro-inflammatory cytokines released in allergy. More recently it was demonstrated that the parasympathetic nervous system (PNS) could also be equally involved in models of peripheral inflammation such as food allergy; although this last clinical presentation...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Favourably effective formulation of sodium iodide and salicylic acid plus professional hygiene in patients affected by desquamative gingivitis.
Authors: Carcieri P, Broccoletti R, Giacometti S, Gambino A, Conrotto D, Cabras M, Arduino PG Abstract The aim of this prospective pilot study was to evaluate the efficiency of an oral hygiene protocol, in combination with a solution of sodium iodide associated to salicylic acid (SISA), in patients affected by desquamative gingivitis (DG). Twenty patients not totally responding to conventional topical therapies, were selected. They received oral hygiene instructions with non-surgical periodontal therapy in a 21-day cohort study (during 3 weekly appointments). The SISA was used at the end of each session, with an im...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Plasma endocannabinoid behaviour in total knee and hip arthroplasty.
Authors: Ottria R, Cappelletti L, Ravelli A, Mariotti M, Gigli F, Romagnoli S, Ciuffreda P, Banfi G, Drago L Abstract Endocannabinoids are a class of lipid mediators involved in a wide range of physiological pathways including pain perception, and immunological defences. In particular, the involvement of endocannabinoids in bone metabolism and bone resorption has recently been studied. Moreover, one study on total knee arthroplasty describes the probable role of endocannabinoids in pain perception after surgery. The aim of the present study was to evaluate variations of endocannabinoid concentrations in patients un...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Minoxidil dose response study in female pattern hair loss patients determined to be non-responders to 5% topical minoxidil.
In this study, we recruited FPHL subjects that were identified as non-responders to 5% topical minoxidil utilizing the previously validated assay for minoxidil response. Subjects were treated for 12 weeks with a novel 15% topical minoxidil solution. At 12 weeks, 60% of subjects achieved a clinically significant response based on target area hair counts (>13.7% from baseline), as well as significant improvement in global photographic assessment. None of the subjects experienced significant hemodynamic changes or any other adverse events. To the best of our knowledge, this is the first study to demonstrate the potentially...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

High level laser therapy for the treatment of lower back pain: clinical efficacy and comparison of different wavelengths.
Authors: Notarnicola A, Maccagnano G, Tafuri S, Gallone MF, Moretti L, Moretti B Abstract High energy laser therapy (HELT) could be a new alternative treatment for lower back pain (LBP), which is a significant public health problem. Nevertheless, differences between the various light waves of HELT have not yet been fully researched. Sixty-six patients with lower back pain were treated using a high energy laser therapy. They were randomized into three different protocols which differed according to wave length (650 nm, 810 nm and TRIAX, which is simultaneous emission of 810 nm, 980 nm e 1064 nm). The other parameter...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Assessing the association between 25-OH vitamin D levels and ROMA score in a population of obese women.
This study suggests that the deficiency of 25- OH vitamin D in obese women has a possible correlation with high ROMA score. PMID: 28078870 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Anti-plaque and antimicrobial efficiency of different oral rinses in a 3-day plaque accumulation model.
Authors: Cantore S, Ballini A, Mori G, Dibello V, Marrelli M, Mirgaldi R, De Vito D, Tatullo M Abstract The idea of incorporating a mouthrinse with normal tooth brushing could be a useful adjunct to oral hygiene. Despite the principle nature of the toothpaste vehicle, most alcohol-based chemical plaque-control agents have been evaluated and later formulated in the mouthrinse vehicle. The current study was aimed to investigate the persistence of antimicrobial action and plaque inhibitory properties of a new alcohol-free mouthrinse when compared with positive control, chlorhexidine 0.12% and placebo control, physiolo...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Molecular characterization and antibiotic resistance of salmonella serovars isolated in the Apulia region of Italy.
Authors: Mirgaldi R, Ballini A, Dionisi AM, Luzzi I, Dipalma G, Inchingolo F, De Vito D Abstract During the period January 2013-December 2015, 175 cases of human salmonellosis were reported in the Apulia Region of Italy. The aim of this study was to characterize salmonella strains from the standpoints of serovars prevalence, antimicrobial resistance and clonal origin. The serological typing was performed by agglutination against antisera followed by a multiplex polymerase chain reaction (m-PCR). The obtained results were analyzed following the Kauffmann-White scheme. Susceptibility to antimicrobial agents was teste...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Fine needle aspiration cytology of 650 thyroid nodules operated for multinodular goiter: a cyto-histological correlation based on the new Italian cytological classification (siapec 2014).
Authors: Tartaglia F, Giuliani A, Tromba L, Carbotta S, Karpathiotakis M, Tortorelli G, Pelle F, Merola R, Donello C, Carbotta G, De Anna L, Conzo G, Sorrenti S, Ulisse S Abstract The new Italian cytological classification (SIAPEC 2014) of thyroid nodules, in line with those of Bethesda and BTA-RCPath, replaces the previous TIR3 class with two new classes (TIR3A and TIR3B), which correspond to different risks of malignancy and clinical actions required. The present study was conducted to evaluate the diagnostic accuracy of the new SIAPEC classification as opposed to its previous version (SIAPEC 2007). Preoperative ...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Tendonitis of the rotator cuff treated with extracorporeal shock wave therapy: radiographic monitoring to identify prognostic factors for disintegration.
Authors: Notarnicola A, Moretti L, Maccagnano G, Tafuri S, Moretti B Abstract Extracorporeal shock wave therapy (ESWT) is widely used for calcific tendonitis of the shoulder. The initial rationale for this therapy was to break the calcification, but this effect does not always occur. To date, we do not know how calcifications evolve or why they may be less responsive to the action of the shock waves. One hundred and seventy-four shoulders with calcific tendinitis were prospectively evaluated before and after ESWT, using the radiographic classifications according to Gartner and Heyer, to Bosworth and to Molè....
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Circulating sRAGE in the diagnosis of osteolytic bone metastasis.
Authors: Galliera E, Marazzi MG, Vianello E, Drago L, Luzzati A, Bendinelli P, Maroni P, Tacchini L, Desiderio MA, Corsi Romanelli MM Abstract Despite the clinical importance of metastasis to the skeleton, the diagnostic tools for early detection and monitoring of bone metastasis lack sensitivity and specificity. We evaluated a promising new serum biomarker, the soluble form of the Receptor of Advanced Glycosylated End-products (sRAGE). sRAGE is involved in the Wnt-signaling pathway, and has been reported to reduce the risk of cancer. We investigated the diagnostic potential of sRAGE to improve the detection and mo...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Comparative molecular analysis of bacterial species associated with periodontal disease.
Authors: De Iuliis V, Ursi S, Di Tommaso LM, Caruso M, Marino A, D Ercole S, Caputi S, Sinjari B, Festa F, Macri M, Martinotti S, Vitullo G, Toniato E Abstract Periodontal disease is an inflammatory disorder affecting the supporting teeth structures, including gingiva, periodontal ligament and alveolar bone, causing loss of connective tissue, reabsorption of alveolar bone and formation of periodontal pockets. The aim of this study is to find a correlation between bacterial growth and periodontal disease. Fifty-seven patients aged between 21 and 65 years, median age 46 years, were enrolled. According to gingival poc...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Congenital muscular dystrophy and epilepsy: a prospective case series of pediatric patients.
Authors: Vitaliti G, Pavone P, Romano C, Barbagallo M, Vecchio M, Ledda C, Lubrano R, Falsaperla R Abstract Congenital Muscular Dystrophies (CMDs) can be considered as a heterogeneous group of diseases characterized by marked weakness, generalized hypotonia and joint contractures. They are divided into pure and classical forms, without ocular and cerebral involvement, and complex forms, which are associated with cerebral abnormalities. Seizures have rarely been described in the pure forms while they seem to occur more frequently in complex forms. The aim of our study was to evaluate the incidence of seizure in CMD....
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Vitamin a decreases after a maximal incremental stress test in non-professional male runners with low aerobic performance.
Authors: Izzicupo P, Ghinassi B, D'Amico MA, Bucciarelli T, Gallina S, Di Baldassarre A Abstract The data on the effects of aerobic training on plasma antioxidant vitamins are conflicting. Additionally, most studies focus on the oxidative profiles of professional athletes, but limited information is available for amateur athlete populations. The aim of this study was to evaluate the effects of high-intensity exercise on antioxidant vitamins in non-professional runners with varying levels of aerobic power. Eighty-one male runners underwent an incremental test to exhaustion. The study population was then divided into...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Zenker diverticulum in the right side of the neck resembling a thyroid mass at ultrasound.
Authors: Alessandrini S, Samperi I, De Cristofaro F, D'Armiento E, Diacinti D, Pernazza A, Bosco D, Ascoli V, Ulisse S Abstract Zenker’s diverticulum represents the most common form of pharyngo-oesophageal diverticula usually occurring on the left side of the neck. Due to its anatomical proximity to the thyroid, it can mimic a thyroid mass. Here we describe the case of an asymptomatic 49-year-old man referred to the Thyroid Clinic of the Policlinico Umberto I Hospital-“Sapienza” University of Rome for thyroid sonography due to a family history of autoimmune thyroid disease. The patient’s thyroid blood tests did...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Orthodontic forces modulate insulin-like growth factor binding protein-5 changes in gingival crevicular fluid.
Authors: Ballini A, Santacroce L, Cantore S, Saini R, Mastrangelo F, Desiate A, Scacco S Abstract Orthodontic tooth movement results from the response of the periodontal tissue to orthodontic force, which leads to modeling and remodeling of the surrounding alveolar bone. The response is considered to occur through the activation of specific signaling pathways, many of which are known, all acting to ultimately result in tooth movement. Much is known about the actions of these two cells, and the signaling pathways that affect them, both in bone and orthodontic literature, however, to date, little work has been carrie...
Source: Journal of Biological Regulators and Homeostatic Agents - January 14, 2017 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Effect of nerve growth factor on cultured human chondrocytes.
This study analysed effects of different NGF concentrations on cultured human chondrocytes by evaluating cell proliferation, cell phenotype, and gene expression. The MTT test excluded an influence on cell viability. Alcian blue and S100 staining demonstrated that NGF may induce de-differentiation of the chondrocyte phenotype. Real-time PCR showed that NGF did not influence gene expression of type I, II and XI collagen, TGF-β, IGF-1 and metalloproteinase (MMP)-13, while it reduced the expression of MMP-3. These findings show that NGF may have uncertain effects in human chondrocytes. Further investigations by wider gene...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Hybrid complexes of high and low molecular weight: evaluation using an in vitro model of osteoarthritis.
Authors: Stellavato A, De Novellis V, Reale S, De Rosa M, Schiraldi C Abstract Hyaluronan (HA) is central in joint and cartilage functions and to restore synovial fluid viscosity. In patients with osteoarthritis (OA), molecular weight (MW) and concentration of hyaluronic acid (HA) are reduced, diminishing joint lubrication. IL-1β treatment was used to mimic osteoarthritis in a chondrocytes based in vitro model. The aim of our research, using this model and human chondrocytes was to assess the anti-inflammatory effect of H/L-HA hybrid complexes (SINOVIAL-HL®) in comparison with HA at high (H-HA) and low mol...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Comparing hybrid hyaluronic acid with PRP in end career athletes with degenerative cartilage lesions of the knee.
Authors: Papalia R, Zampogna B, Russo F, Vasta S, Tirindelli MC, Nobile C, Di Martino AC, Vadalà G, Denaro V Abstract Cartilage lesions are very common causes of chronic knee pain in athletes. Current treatment options consist in conservative strategies, such as viscosupplementation and platelet-rich plasma injections. This randomized controlled trial aims to investigate the effect of intra-articular Hybrid Hyaluronic Acid injections compared to PRP for the treatment of cartilage lesions among athletes at the end of their career. Since March 2015, 48 professional soccer players were randomized into two group...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Comparison of three novel biphasic scaffolds for one-stage treatment of osteochondral defects in a sheep model.
Authors: Gervaso F, Mangiavini L, Di Giancamillo A, Boschetti F, Izzo D, Zani DD, Di Giancamillo M, Tessaro I, Domenicucci M, Scalera F, Domeneghini C, Crovace AM, Sannino A, Peretti GM Abstract In the last years, several tissue engineering techniques have been applied to develop different kinds of osteochondral substitutes to overcome the scarce reparative properties of this tissue. The aim of this study was to generate and compare three biphasic scaffolds in an osteochondral lesion in a large-animal model. A critical osteochondral defect was generated in the medial femoral condyle of 18 skeletally mature sheep. T...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Tranexamic acid effects on cartilage and synovial tissue: an in vitro study for a possible safe intra-articular use.
This study demonstrated that TA does not negatively affect chondrocytes and synoviocytes cultured in vitro. Thus, our findings may be clinically relevant in order to validate the intra-articular TA administration during orthopedic procedures. PMID: 28002898 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Intraoperative application Platelet rich fibrin, postoperative injections OF PRP or microfracture only for osteochondral lesions of the knee: a five-year retrospective evaluation.
Authors: Papalia R, Diaz Balzani L, Torre G, Tirindelli MC, Nobile C, Maffulli N, Denaro V Abstract Cartilage lesions are the most common cause of chronic knee pain. Micro-fracturing is reliable, effective, easy to perform and inexpensive. We propose a novel approach to cartilage lesions where microfractures are performed contextually to intra-operative or post-operative administration of platelet concentrates. We retrospectively evaluate 48 patients divided in 3 groups. Group 1: 15 patients underwent microfractures and intraoperative administration of PRF (PRF group); group 2: 16 microfractures and postoperative i...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Pain reduction and improvement of function following ultrasound-guided intra-articular injections of triamcinolone hexacetonide and hyaluronic acid in hip osteoarthritis.
This study shows that ultrasound-guided intra-articular injections of triamcinolone hexacetonide and hyaluronic acid can improve pain, function and quality of life in patients with symptomatic and radiographic hip osteoarthritis. In addition, the administration of triamcinolone hexacetonide and hyaluronic acid to the hip joint in these patients can delay the need for interventional surgery. PMID: 28002900 [PubMed - in process] (Source: Journal of Biological Regulators and Homeostatic Agents)
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Homologous platelet-rich plasma for the treatment of knee involvement in primary Sj ögren’s syndrome.
Homologous platelet-rich plasma for the treatment of knee involvement in primary Sjögren’s syndrome. J Biol Regul Homeost Agents. 2016 Oct-Dec;30(4 Suppl 1):63-67 Authors: Bottegoni C, Farinelli L, Aquili A, Chiurazzi E, Gigante A Abstract Primary Sjögren’s syndrome (pSS) is a chronic autoimmune disease characterized by dry eyes, dry mouth, and other clinical manifestations. The most common extraglandular manifestation of pSS is articular involvement and to date their management is unclear. The aims of the current pilot study were to assess the safety and the outcomes of homologous platelet...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Bone marrow concentrated cells and stromal vascular fraction cells injections for osteoarthritis treatment: a systematic review.
Authors: Vadalà G, Papalia R, La Verde L, Russo F, Denaro V, Rosa MA Abstract The aim of this systematic review is to examine current clinical evidences supporting the intraarticular injection of bone marrow concentrate cells (BMC) and adipose-derived stromal vascular fraction cells (SVF) for the treatment of osteoarthritis (OA). The research was performed on PubMed (Medline), EMBASE and Cochrane Library considering the English literature. Only clinical trials have been included. The systematic research identified twelve clinical trials. Articles included in the study, were one of level II, four of level III...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

The cytokinome in osteoarthritis, a new paradigm in diagnosis and prognosis of cartilage disease.
Authors: Papalia R, Vadalà G, Torre G, Perna M, Saccone L, Cannata F, Denaro V Abstract At present, diagnosis and progression monitoring of osteoarthritis (OA) is made through radiological and clinical assessment. Several studies investigated the role of synovial fluid analysis, to find out whether joint disease could be characterized by the pattern of cytokines, which acts during the pathogenic process or in specific stages of it. Online PubMed-Medline search was performed in order to retrieve evidence concerning synovial fluid analysis of cytokines involved in OA degenerative process. Concerning pro-inflam...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

The meniscus vascularization: the direct correlation with tissue composition for tissue engineering purposes.
Authors: Di Giancamillo A, Mangiavini L, Tessaro I, Marmotti A, Scurati R, Peretti GM Abstract Meniscal lesions still represent an unsolved problem in clinical practice. Like the articular cartilage, meniscus has a scarce healing potential. Thus, when this tissue is damaged, the joint biomechanics is completely altered, leading to the development and progression of premature osteoarthritis. Therefore, in the last years, several tissue-engineering strategies have been developed to regenerate the meniscus with debated results. The comprehension of complex processes underlying meniscus maturation and structure is esse...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Arthroscopic all-inside treatment of popliteomeniscal fascicles tears: surgical technique and results from the first 6 consecutive patients.
Authors: Simonetta R, Di Vico G, Papalia R, Vasta S, Denaro V Abstract Athletes whose knees are subjected to sudden changes of direction and high jumps such as martial arts athletes, dancers, wrestlers and football players are at higher risk of injuring popliteomeniscal fascicles. Painful squatting and mechanical symptoms such as locking sensation are common. Current available treatments includes open or arthroscopic in repair. Arthroscopic repair with all-inside device can relieve symptoms and restore knee function. Six patients from two surgical centers with isolated popliteomeniscal fascicles tears were treated ...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Tears of popliteomeniscal fascicles, diagnostic and clinical implications. A review of the evidence.
Authors: Papalia R, Simonetta R, Di Vico G, Torre G, Saccone L, Espregueira-Mendes J, Denaro V Abstract Postero-lateral corner of the knee is composed of several structures including the popliteo-meniscal fascicles (PMFs). These fibrous structures form a stable ligamentous complex around the popliteus tendon, which stabilize the lateral meniscus, increasing the strength of postero-lateral corner. Studies were retrieved through an electronic search of CINAHL, EMBASE, and Pub-Med, until May 2016. Studies in English, Italian, French, and Spanish were considered for inclusion. Randomized controlled trials, prospective ...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

TGF- β1 differentially modulates the collagen VI α5 and α6 chains in human tendon cultures.
TGF-β1 differentially modulates the collagen VI α5 and α6 chains in human tendon cultures. J Biol Regul Homeost Agents. 2016 Oct-Dec;30(4 Suppl 1):107-113 Authors: Sabatelli P, Sardone F, Traina F, Merlini L, Santi S, Wagener R, Faldini C Abstract Collagen VI is a microfibrillar collagen with a potential regulatory role in tendon repair mechanism. We studied the expression of collagen VI α5 and α6 chains in normal human tendon fibroblast cultures, both under basal condition and in response to TGF-β1, a potent regulator of tendon healing. Under basal condition, we found...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Heat shock induces the expression of pro-inflammatory cytokines in human Achilles tendon tenocytes.
Authors: D'Addona A, Somma D, Formisano S, Rosa D, Di Donato SL, Maffulli N Abstract The aim of our study is to investigate the behaviour of healthy and tendinopathic human tenocytes after a heat shock. After we harvested tendinopathic and healthy human tendon samples, we split tenocytes into 4 groups: 3 groups were submitted to heat shock, followed by different periods of post-heating (2, 4 and 20 h). The other group represents our negative control. The target genes were analysed using Real Time PCR. IL-1β and IL-6 expression were significantly increased in tendinopathic samples after heat shock. COL1 and COL...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Risk factors for post-operative shoulder stiffness: are there new candidates?
Authors: Cucchi D, Menon A, Feroldi FM, Kwapisz A, Randelli F, Cabitza F, Tassi A, Randelli P Abstract The aim of this study was to document the incidence of postoperative shoulder stiffness (SS) after arthroscopic rotator cuff repair and evaluate the role of risk factors for its development. Seventy-five consecutive patients that underwent arthroscopic rotator cuff repair were included. The incidence of postoperative SS was prospectively investigated and the presence of 20 potential risk factors was documented retrospectively. The incidence of post-operative SS was 10.4%. All patients were women, and sex was signi...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Surgical treatment of insertional Achilles tendinopathy: a systematic review.
Authors: Traina F, Ruffilli A, Mazzotti A, Meliconi R, Berti L, Faldini C Abstract Insertional Achilles tendinopathy is a frequent cause of pain and performance impairment of the ankle. It is more common in runners, but may also affect general population. Conservative treatment is the gold standard in the early phases but 10% to 30% of patients require surgery. The aim of this study is to review the current literature in order to evaluate current surgical strategies for Insertional Achilles tendinopathy and to analyze the effectiveness of the available techniques. We performed a systematic review of the literature,...
Source: Journal of Biological Regulators and Homeostatic Agents - December 25, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research