Loss of function of XBP1 splicing activity of IRE1 α favors B cell tolerance breakdown
J Autoimmun. 2023 Dec 9;142:103152. doi: 10.1016/j.jaut.2023.103152. Online ahead of print.ABSTRACTAnti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. By Whole-Exome Sequencing, we identified a new heterozygous mutation (p.R594C) in ERN1 gene, encoding IRE1α (Inositol-Requiring E...
Source: Journal of Autoimmunity - December 10, 2023 Category: Allergy & Immunology Authors: Quentin Reuschl é Laurien Van Heddegem Victor Bosteels Matthieu Moncan Sabine Depauw Nad ège Wadier Sandra Mar échal Clint De Nolf Virginia Delgado Yosra Messai Marie-Claude Stolzenberg Aude Mag érus Ang élique Werck J érôme Olagne Quan Li Guillaum Source Type: research
Loss of function of XBP1 splicing activity of IRE1 α favors B cell tolerance breakdown
J Autoimmun. 2023 Dec 9;142:103152. doi: 10.1016/j.jaut.2023.103152. Online ahead of print.ABSTRACTAnti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. By Whole-Exome Sequencing, we identified a new heterozygous mutation (p.R594C) in ERN1 gene, encoding IRE1α (Inositol-Requiring E...
Source: Journal of Autoimmunity - December 10, 2023 Category: Allergy & Immunology Authors: Quentin Reuschl é Laurien Van Heddegem Victor Bosteels Matthieu Moncan Sabine Depauw Nad ège Wadier Sandra Mar échal Clint De Nolf Virginia Delgado Yosra Messai Marie-Claude Stolzenberg Aude Mag érus Ang élique Werck J érôme Olagne Quan Li Guillaum Source Type: research
Loss of function of XBP1 splicing activity of IRE1 α favors B cell tolerance breakdown
J Autoimmun. 2023 Dec 9;142:103152. doi: 10.1016/j.jaut.2023.103152. Online ahead of print.ABSTRACTAnti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. By Whole-Exome Sequencing, we identified a new heterozygous mutation (p.R594C) in ERN1 gene, encoding IRE1α (Inositol-Requiring E...
Source: Journal of Autoimmunity - December 10, 2023 Category: Allergy & Immunology Authors: Quentin Reuschl é Laurien Van Heddegem Victor Bosteels Matthieu Moncan Sabine Depauw Nad ège Wadier Sandra Mar échal Clint De Nolf Virginia Delgado Yosra Messai Marie-Claude Stolzenberg Aude Mag érus Ang élique Werck J érôme Olagne Quan Li Guillaum Source Type: research
Loss of function of XBP1 splicing activity of IRE1 α favors B cell tolerance breakdown
J Autoimmun. 2023 Dec 9;142:103152. doi: 10.1016/j.jaut.2023.103152. Online ahead of print.ABSTRACTAnti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. By Whole-Exome Sequencing, we identified a new heterozygous mutation (p.R594C) in ERN1 gene, encoding IRE1α (Inositol-Requiring E...
Source: Journal of Autoimmunity - December 10, 2023 Category: Allergy & Immunology Authors: Quentin Reuschl é Laurien Van Heddegem Victor Bosteels Matthieu Moncan Sabine Depauw Nad ège Wadier Sandra Mar échal Clint De Nolf Virginia Delgado Yosra Messai Marie-Claude Stolzenberg Aude Mag érus Ang élique Werck J érôme Olagne Quan Li Guillaum Source Type: research
Loss of function of XBP1 splicing activity of IRE1 α favors B cell tolerance breakdown
J Autoimmun. 2023 Dec 9;142:103152. doi: 10.1016/j.jaut.2023.103152. Online ahead of print.ABSTRACTAnti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. By Whole-Exome Sequencing, we identified a new heterozygous mutation (p.R594C) in ERN1 gene, encoding IRE1α (Inositol-Requiring E...
Source: Journal of Autoimmunity - December 10, 2023 Category: Allergy & Immunology Authors: Quentin Reuschl é Laurien Van Heddegem Victor Bosteels Matthieu Moncan Sabine Depauw Nad ège Wadier Sandra Mar échal Clint De Nolf Virginia Delgado Yosra Messai Marie-Claude Stolzenberg Aude Mag érus Ang élique Werck J érôme Olagne Quan Li Guillaum Source Type: research
Loss of function of XBP1 splicing activity of IRE1 α favors B cell tolerance breakdown
J Autoimmun. 2023 Dec 9;142:103152. doi: 10.1016/j.jaut.2023.103152. Online ahead of print.ABSTRACTAnti-nuclear antibodies are the hallmark of autoimmune diseases such as systemic lupus erythematosus (SLE) and scleroderma. However, the molecular mechanisms of B cell tolerance breakdown in these pathological contexts are poorly known. The study of rare familial forms of autoimmune diseases could therefore help to better describe common biological mechanisms leading to B cell tolerance breakdown. By Whole-Exome Sequencing, we identified a new heterozygous mutation (p.R594C) in ERN1 gene, encoding IRE1α (Inositol-Requiring E...
Source: Journal of Autoimmunity - December 10, 2023 Category: Allergy & Immunology Authors: Quentin Reuschl é Laurien Van Heddegem Victor Bosteels Matthieu Moncan Sabine Depauw Nad ège Wadier Sandra Mar échal Clint De Nolf Virginia Delgado Yosra Messai Marie-Claude Stolzenberg Aude Mag érus Ang élique Werck J érôme Olagne Quan Li Guillaum Source Type: research
Associations between CD70 methylation of T cell DNA and age in adults with systemic lupus erythematosus and population controls: The Michigan Lupus Epidemiology & amp; Surveillance (MILES) Program
CONCLUSIONS: We describe altered CD70 methylation patterns in T lymphocyte subsets in adults with SLE relative to controls, and report associations particular to SLE between methylation of this immune-relevant gene and both age and race, possibly a consequence of "weathering" or accelerated aging which may have implications for SLE pathogenesis and potential intervention strategies.PMID:38064919 | DOI:10.1016/j.jaut.2023.103137 (Source: Journal of Autoimmunity)
Source: Journal of Autoimmunity - December 8, 2023 Category: Allergy & Immunology Authors: Emily C Somers Jaclyn M Goodrich Lu Wang Sioban D Harlow Wendy Marder Afton L Hassett Suzanna M Zick W Joseph McCune Caroline Gordon Kamil E Barbour Charles G Helmick Faith M Strickland Source Type: research
Associations between CD70 methylation of T cell DNA and age in adults with systemic lupus erythematosus and population controls: The Michigan Lupus Epidemiology & amp; Surveillance (MILES) Program
CONCLUSIONS: We describe altered CD70 methylation patterns in T lymphocyte subsets in adults with SLE relative to controls, and report associations particular to SLE between methylation of this immune-relevant gene and both age and race, possibly a consequence of "weathering" or accelerated aging which may have implications for SLE pathogenesis and potential intervention strategies.PMID:38064919 | DOI:10.1016/j.jaut.2023.103137 (Source: Journal of Autoimmunity)
Source: Journal of Autoimmunity - December 8, 2023 Category: Allergy & Immunology Authors: Emily C Somers Jaclyn M Goodrich Lu Wang Sioban D Harlow Wendy Marder Afton L Hassett Suzanna M Zick W Joseph McCune Caroline Gordon Kamil E Barbour Charles G Helmick Faith M Strickland Source Type: research
High-resolution HLA genotyping in inclusion body myositis refines 8.1 ancestral haplotype association to DRB1*03:01:01 and highlights pathogenic role of arginine-74 of DR β1 chain
CONCLUSIONS: High-resolution HLA sequencing more precisely characterised the alleles associated with IBM and defined a haplotype linked to earlier disease onset. Identification of the critical amino acid residue by advanced biostatistical analysis of immunogenetics data offers mechanistic insights and future directions into uncovering IBM aetiopathogenesis.PMID:38043487 | DOI:10.1016/j.jaut.2023.103150 (Source: Journal of Autoimmunity)
Source: Journal of Autoimmunity - December 3, 2023 Category: Allergy & Immunology Authors: Nataliya Slater Anuradha Sooda Emily McLeish Kelly Beer Anna Brusch Rakesh Shakya Christine Bundell Ian James Abha Chopra Frank L Mastaglia Merrilee Needham Jerome D Coudert Source Type: research
High-resolution HLA genotyping in inclusion body myositis refines 8.1 ancestral haplotype association to DRB1*03:01:01 and highlights pathogenic role of arginine-74 of DR β1 chain
CONCLUSIONS: High-resolution HLA sequencing more precisely characterised the alleles associated with IBM and defined a haplotype linked to earlier disease onset. Identification of the critical amino acid residue by advanced biostatistical analysis of immunogenetics data offers mechanistic insights and future directions into uncovering IBM aetiopathogenesis.PMID:38043487 | DOI:10.1016/j.jaut.2023.103150 (Source: Journal of Autoimmunity)
Source: Journal of Autoimmunity - December 3, 2023 Category: Allergy & Immunology Authors: Nataliya Slater Anuradha Sooda Emily McLeish Kelly Beer Anna Brusch Rakesh Shakya Christine Bundell Ian James Abha Chopra Frank L Mastaglia Merrilee Needham Jerome D Coudert Source Type: research
High-resolution HLA genotyping in inclusion body myositis refines 8.1 ancestral haplotype association to DRB1*03:01:01 and highlights pathogenic role of arginine-74 of DR β1 chain
CONCLUSIONS: High-resolution HLA sequencing more precisely characterised the alleles associated with IBM and defined a haplotype linked to earlier disease onset. Identification of the critical amino acid residue by advanced biostatistical analysis of immunogenetics data offers mechanistic insights and future directions into uncovering IBM aetiopathogenesis.PMID:38043487 | DOI:10.1016/j.jaut.2023.103150 (Source: Journal of Autoimmunity)
Source: Journal of Autoimmunity - December 3, 2023 Category: Allergy & Immunology Authors: Nataliya Slater Anuradha Sooda Emily McLeish Kelly Beer Anna Brusch Rakesh Shakya Christine Bundell Ian James Abha Chopra Frank L Mastaglia Merrilee Needham Jerome D Coudert Source Type: research
High-resolution HLA genotyping in inclusion body myositis refines 8.1 ancestral haplotype association to DRB1*03:01:01 and highlights pathogenic role of arginine-74 of DR β1 chain
CONCLUSIONS: High-resolution HLA sequencing more precisely characterised the alleles associated with IBM and defined a haplotype linked to earlier disease onset. Identification of the critical amino acid residue by advanced biostatistical analysis of immunogenetics data offers mechanistic insights and future directions into uncovering IBM aetiopathogenesis.PMID:38043487 | DOI:10.1016/j.jaut.2023.103150 (Source: Journal of Autoimmunity)
Source: Journal of Autoimmunity - December 3, 2023 Category: Allergy & Immunology Authors: Nataliya Slater Anuradha Sooda Emily McLeish Kelly Beer Anna Brusch Rakesh Shakya Christine Bundell Ian James Abha Chopra Frank L Mastaglia Merrilee Needham Jerome D Coudert Source Type: research
High-resolution HLA genotyping in inclusion body myositis refines 8.1 ancestral haplotype association to DRB1*03:01:01 and highlights pathogenic role of arginine-74 of DR β1 chain
CONCLUSIONS: High-resolution HLA sequencing more precisely characterised the alleles associated with IBM and defined a haplotype linked to earlier disease onset. Identification of the critical amino acid residue by advanced biostatistical analysis of immunogenetics data offers mechanistic insights and future directions into uncovering IBM aetiopathogenesis.PMID:38043487 | DOI:10.1016/j.jaut.2023.103150 (Source: Journal of Autoimmunity)
Source: Journal of Autoimmunity - December 3, 2023 Category: Allergy & Immunology Authors: Nataliya Slater Anuradha Sooda Emily McLeish Kelly Beer Anna Brusch Rakesh Shakya Christine Bundell Ian James Abha Chopra Frank L Mastaglia Merrilee Needham Jerome D Coudert Source Type: research
Neointimal myofibroblasts contribute to maintaining Th1/Tc1 and Th17/Tc17 inflammation in giant cell arteritis
This study aimed to investigate if MFs could also impact T-cell polarization. Confocal microscopy was used to analyze fresh fragments of temporal artery biopsies (TABs). Healthy TAB sections were cultured to obtain MFs, which were then treated or not with interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) and analyzed by immunofluorescence and RT-PCR. After peripheral blood mononuclear cells and MFs were co-cultured for seven days, T-cell polarization was analyzed by flow cytometry. In the neointima of GCA arteries, we observed a phenotypic heterogeneity among VSMCs that was consistent with a MF phenotype (...
Source: Journal of Autoimmunity - December 1, 2023 Category: Allergy & Immunology Authors: H élène Greigert Andr é Ramon Coraline Genet Claudie Cladi ère Claire Gerard Marion Cuidad Marc Corbera-Bellalta Roser Alba-Rovira Louis Arnould Catherine Creuzot-Garcher Laurent Martin Georges Tarris Thibault Ghesqui ère Sethi Ouandji Sylvain Audia Source Type: research
Neointimal myofibroblasts contribute to maintaining Th1/Tc1 and Th17/Tc17 inflammation in giant cell arteritis
This study aimed to investigate if MFs could also impact T-cell polarization. Confocal microscopy was used to analyze fresh fragments of temporal artery biopsies (TABs). Healthy TAB sections were cultured to obtain MFs, which were then treated or not with interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) and analyzed by immunofluorescence and RT-PCR. After peripheral blood mononuclear cells and MFs were co-cultured for seven days, T-cell polarization was analyzed by flow cytometry. In the neointima of GCA arteries, we observed a phenotypic heterogeneity among VSMCs that was consistent with a MF phenotype (...
Source: Journal of Autoimmunity - December 1, 2023 Category: Allergy & Immunology Authors: H élène Greigert Andr é Ramon Coraline Genet Claudie Cladi ère Claire Gerard Marion Cuidad Marc Corbera-Bellalta Roser Alba-Rovira Louis Arnould Catherine Creuzot-Garcher Laurent Martin Georges Tarris Thibault Ghesqui ère Sethi Ouandji Sylvain Audia Source Type: research
