Clinical wear of approximal glass ionomer restorations protected with a nanofilled self-adhesive light-cured protective coating
Conclusion: These results suggest that the application of a NPC has a protective effect on the clinical wear of approximal GIC restorations in primary teeth. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

The role of potassium channels in the endothelial dysfunction induced by periodontitis
Conclusions: Periodontitis induces impairment in endothelial-dependent relaxation; this impairment recovers, even in the presence of periodontitis. The recovery is mediated by the activation of endothelial calcium-activated potassium channels in ligature animals. Although important for maintenance of vascular homeostasis, this effect could mask the lack of NO, which has other beneficial properties. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

Pulp analysis of teeth submitted to different types of forces: a histological study in rats
Conclusions: Pulp tissue alterations resulting from ITM were limited to hemodynamic events, without progressing to irreversible degeneration, regardless of the type of force applied. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

Effects of experimental bleaching agents on the mineral content of sound and demineralized enamels
Abstract High concentrations of hydrogen peroxide can cause adverse effects on composition and structure of teeth. However, the addition of calcium and fluoride in bleaching agents may reduce enamel demineralization. Objective: To evaluate chemical changes of sound and demineralized enamels submitted to high concentrations of hydrogen peroxide containing fluoride (F) or calcium (Ca). Material and Methods: Enamel blocks of bovine incisors with standard dimensions were obtained and half of them were submitted to pH-cycling to promote initial enamel caries lesions. Sound and demineralized enamel samples were divided into (n=1...
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

Quorum sensing LuxS/autoinducer-2 inhibits Enterococcus faecalis biofilm formation ability
Abstract Objective: To investigate the relation between biofilm formation ability and quorum sensing gene LuxS/AI-2. Materials and Methods: Enterococcus faecalis (E. faecalis) standard strain ATCC 29212 was used in the study. Long flanking homology polymerase chain reaction method was used to build the LuxS gene knockout strain. Sequential culture turbidity measurement and CFU counting were used to assess the proliferation ability of E. faecalis after the depletion of LuxS. 96-well plate assay was used to quantify the biofilm formation ability; CLSM was used to observe the attached bacteria areas, while scanning electron m...
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis
Abstract Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN- γ ) levels are high in CP patients; however, the IFN- γ receptor (IFN- γ R) has not been studied in gingival tissue from these patients. Objective: To evaluate IFN- γ levels and IFN- γ R expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). Material and Methods: Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each s...
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

Volumetric reconstruction and determination of minimum crosssectional area of the pharynx in patients with cleft lip and palate: comparison between two different softwares
Conclusion: Measurements of volume and mCSA are statistically similar between applications. Therefore, open-source applications may be a viable option to assess upper airway dimensions using CBCT exams. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

One-year prospective clinical study comparing patient satisfaction and masticatory performance of mandibular overdentures supported by one versus two implants
Abstract There is a lack of clinical evidence on mandibular overdentures (MOD) supported by a single implant. Objective: To compare patient satisfaction and masticatory performance in MOD supported by one versus two implants in a two-group parallel randomized clinical trial. Material and Methods: Twenty-one patients wearing new maxillary and mandibular complete dentures (CDs) were randomly divided to receive one (GI, n = 11) or two (GII, n = 10) implants in the mandibular arch. Four months after implant placement, o-ring abutments were installed in the implants, and matrix attachments were placed in the lower complete dent...
Source: Journal of Applied Oral Science - October 4, 2018 Category: Dentistry Source Type: research

Cyclic flexural fatigue resistance of NiTi Controlled Memory and Blue Technology instruments after torsional preloading
Abstract Objective The aim of this study was to evaluate the influence of torsional preloading on the cyclic flexural fatigue resistance of thermally treated NiTi instruments. Material and Methods Ten new instruments New Hyflex CM (HF 30.06; Coltene/Whaladent Inc.), Typhoon CM (TYP 30.06; Clinician's Choice Dental Products) and Vortex Blue (VB 30.06; Dentsply Tulsa Dental) were chosen, based on geometry and specific characteristics of the manufacturing process. The new instruments of each system were tested in a bench device to determine their fatigue resistance through mean value of number of cycles to failure (Nf) (Contr...
Source: Journal of Applied Oral Science - August 21, 2018 Category: Dentistry Source Type: research

Effect of cavity preparation design and ceramic type on the stress distribution, strain and fracture resistance of CAD/CAM onlays in molars
This study aimed to evaluate the effect of the cavity preparation and ceramic type on the stress distribution, tooth strain, fracture resistance and fracture mode of human molar teeth restored with onlays. Material and Methods Forty-eight molars were divided into four groups (n=12) with assorted combinations of two study factors: BL- conventional onlay preparation with boxes made from leucite ceramic (IPS-Empress CAD, Ivoclar Vivadent); NBL- conservative onlay preparation without boxes made from leucite ceramic; BD- conventional onlay preparation with boxes made from lithium disilicate glass ceramic (IPS e.max CAD, Ivoclar...
Source: Journal of Applied Oral Science - August 21, 2018 Category: Dentistry Source Type: research

Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials
The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubi...
Source: Journal of Applied Oral Science - August 21, 2018 Category: Dentistry Source Type: research

Relationship between phonological working memory, metacognitive skills and reading comprehension in children with learning disabilities
Conclusion children with learning disabilities presented deficits in phonological working memory and use of metacognitive strategies. The positive and effective correlation between the abilities analyzed suggests that failure in the phonological working memory and use of metacognitive strategies interfere with reading comprehension. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - August 21, 2018 Category: Dentistry Source Type: research

Effect of non-surgical periodontal therapy on the degree of gingival inflammation and stress markers related to pregnancy
Abstract Objectives The purpose of this study was to determine the impact of nonsurgical periodontal therapy considering the salivary stress-related hormone and cytokine levels in the gingival crevicular fluid (GCF) on pregnant and nonpregnant women. Material and Methods Thirty non-pregnant (control group) and 30 pregnant women (test group) that met the study inclusion criteria were chosen. Only participants with gingivitis were included. Clinical data and samples of GCF and saliva were collected at baseline and after periodontal therapy. The levels of interleukin-1 beta ( Κ -1 β ) and IL-10, and concentration o...
Source: Journal of Applied Oral Science - July 23, 2018 Category: Dentistry Source Type: research

The association between periodontal conditions, inflammation, nutritional status and calcium-phosphate metabolism disorders in hemodialysis patients
Conclusions High prevalence and severity of periodontal disease are observed in hemodialysis patients. There is a high probability that periodontal disease may be present at the early stages of chronic kidney disease (CKD) before the hemodialysis onset. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 23, 2018 Category: Dentistry Source Type: research

Relationship between phonological working memory, metacognitive skills and reading comprehension in children with learning disabilities
Conclusion children with learning disabilities presented deficits in phonological working memory and use of metacognitive strategies. The positive and effective correlation between the abilities analyzed suggests that failure in the phonological working memory and use of metacognitive strategies interfere with reading comprehension. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 23, 2018 Category: Dentistry Source Type: research

Tomographic late evaluation of xenogeneic bone grafts in sockets of impacted third molars
The objective of this study was to evaluate the distance of the crest of alveolar ridge to the cementoenamel junction (CEJ) of the lower second molars and the bone density of the third molar socket filled with Gen-Tech ®, 5 years after an exodontia using cone beam computed tomography (CBCT) to visualize the central region of the sockets, without overlapping of the buccal and lingual cortical bones. Material and Methods A total of 12 individuals from an initial group of 39 patients submitted to extraction of the unruptured lower third molars and grafting of an association of inorganic bovine bone matrix, organic bovine ...
Source: Journal of Applied Oral Science - July 16, 2018 Category: Dentistry Source Type: research

Study of the chemical interaction between a high-viscosity glass ionomer cement and dentin
Conclusions The analysis revealed the formation of new compounds or molecular rearrangements resulting from the chemical interactions between GIC and dentin. Moreover, this study provides an effective method to evaluate the dynamics of the setting mechanism of GICs. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 16, 2018 Category: Dentistry Source Type: research

Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model
Conclusions Within the limits of this study, we suggest that diode laser application is an adjunctive treatment because it reduced inflammation and MPO when applied in addition to SRP. On the other hand, more studies are needed for the assessment of the effects of diode laser application to periodontal tissues. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 16, 2018 Category: Dentistry Source Type: research

Communicative performance and vocabulary domain in preschool preterm infants
Conclusion Children born prematurely with low risk of neurological sequelae in preschool age may have greater difficulties in linguistic performance than their peers born to term. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 16, 2018 Category: Dentistry Source Type: research

ERRATUM: Neurosensory analysis of tooth sensitivity during at-home dental bleaching: a randomized clinical trial
Abstract Clear cell odontogenic carcinoma (CCOC) is a rare odontogenic tumor of the jaws, histologically characterized by the presence of agglomerates of cells with eosinophilic cytoplasm. The patient, a 62-year-old Caucasian woman, presented an intraosseous lesion in the mandibular symphysis. A clinical examination revealed a discrete volumetric increase with a hard consistency, palpable to extraoral and intraoral examinations. Imaging studies revealed an extensive radiolucent area, without defined limits, extending from the region of the right second premolar to the left canine. Incisional biopsy analysis indicated a dia...
Source: Journal of Applied Oral Science - July 10, 2018 Category: Dentistry Source Type: research

Effects of hyperbaric oxygen treatment on implant osseointegration in experimental diabetes mellitus
Conclusion Histomorphometry findings suggest that HBO has positive effect on implant osseointegration in the early healing period in diabetic rabbits. However, implant stability is not affected by HBO treatment. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 10, 2018 Category: Dentistry Source Type: research

Effects of platelet-rich plasma on human gingival fibroblast proliferation and migration in vitro
Conclusion In this study, perhaps the medium with 5% PRP is the dominant option, promoting the abilities of hGFs to heal wounds, because of its fast and effective impact on cell proliferation, colony formation and migration. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 10, 2018 Category: Dentistry Source Type: research

Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin
Conclusion Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implants. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 10, 2018 Category: Dentistry Source Type: research

Immunohistochemical and mRNA expression of RANK, RANKL, OPG, TLR2 and MyD88 during apical periodontitis progression in mice
Abstract Objective To evaluate and correlate, in the same research, the mRNA expression and the staining of RANK, RANKL, OPG, TLR2 and MyD88 by immunohistochemistry in the apical periodontitis (AP) progression in mice. Material and Methods AP was induced in the lower first molars of thirty-five C57BL/6 mice. They were assigned to four groups according to their euthanasia periods (G0, G7, G21 and G42). The jaws were removed and subjected to histotechnical processing, immunohistochemistry and real-time reverse transcription-PCR (qRT-PCR). Data were analyzed with parametric and nonparametric tests ( α =0.05). Results An...
Source: Journal of Applied Oral Science - July 10, 2018 Category: Dentistry Source Type: research

Daily melatonin administration improves osseointegration in pinealectomized rats
Conclusion The absence of the pineal gland impaired the bone repair process during osseointegratio n, however the daily melatonin replacement was able to restore this response. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - July 10, 2018 Category: Dentistry Source Type: research

Myofascial trigger points in patients with temporomandibular joint disc displacement with reduction: a cross-sectional study
The objective of this retrospective study was to evaluate the impact of myofascial trigger points (MTrPs) in patients with articular disc displacement with reduction (DDWR) and to identify which clinical variables are associated with the concomitant presence of DDWR and MTrPs. Material and Methods 130 patients were selected that sought treatment due to joint pain, with ages ≥ 18 years, of both genders, with DDWR confirmed by magnetic resonance imaging. The sample was divided into two groups: Group 1, patients with DDWR and MTrPs (N=101); and Group 2, patients with DDWR and no MTrPs (N=29). Information on gender, age, pa...
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Long-term microwaving of denture base materials: effects on dimensional, color and translucency stability
Abstract While the combined effect of microwave irradiation with cleansing solutions on denture base materials has been investigated, the effects of only using microwave irradiation and, more importantly, in a long-term basis, was not studied yet. Objective The purpose of this study was to evaluate the effect of a long-term repeated microwaving on the dimensional, color and translucency stability of acrylic and polyamide denture base materials. Material and Methods Thirty two specimens (32 mm x 10 mm x 2.5 mm) from polyamide (Valplast) and PMMA (Vertex Rapid Simplified) denture base materials were made. Eight specimens fro...
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Maxillomandibular giant osteosclerotic lesions
Conclusions GOLs must be differentiated from other radiopaque benign and malignant tumors. Condensing osteitis, was considered an anomalous osseous response induced by a chronic low-grade inflammatory stimulus. For development of idiopathic osteosclerosis, two possible mechanisms could be related. The first is modification of the normal turnover with excessive osseous deposition. The second mechanism will prevent the normal bone resorption, arresting the osseous breakdown process. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Minimization of polymerization shrinkage effects on composite resins by the control of irradiance during the photoactivation process
Conclusion The results from this study can pr omote the improvement of the composite resin light curing process by the minimization of polymerization shrinkage effects, given an operational standardization of the photoactivation process. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Changes in bacterial number at different sites of oral cavity during perioperative oral care management in gastrointestinal cancer patients: preliminary study
Conclusions Even with regular oral health care, bacterial numbers remained high in the upper incisor tooth gingiva in gastrointestinal cancer patients who received open surgery. Additional procedures are likely needed to effectively reduce the number of bacteria in the gingival area associated with the upper anterior teeth. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis
Conclusion Micro-CT showed the newly formed bone had favorable morphometric characteristics of quality and quantity. Beyond the RUNX2, OC, OPN, OST, and RANK proteins expressed in the alveolar bone healing, OPG and RANKL activity showed to be essential for activation of basic multicellular units during the alveolar bone healing. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Efficacy of relacin combined with sodium hypochlorite against Enterococcus faecalis biofilms
Abstract Objective Relacin is a synthetic molecule that targets RelA, an essential protein in a conserved bacterial stress response system. It was shown to inhibit bacterial growth. The aims of this study were to evaluate the antimicrobial effect of relacin combined with sodium hypochlorite (NaOCl) on Enterococcus faecalis biofilms and to evaluate the cytotoxicity of relacin. Material and Methods 48-h E. faecalis OG1RF biofilms were treated by various concentrations of relacin in order to determine its inhibitory concentration. Then, the 48-h biofilms were treated either with 1-min NaOCl (0.01%, 0.05%) alone, or in combina...
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Oral implant osseointegration model in C57Bl/6 mice: microtomographic, histological, histomorphometric and molecular characterization
Conclusions: This study demonstrated a unique molecular view of oral osseointegration kinetics in C57Bl/6 mice, evidencing potential elements responsible for orchestrating cell migration, proliferation, ECM deposition and maturation, angiogenesis, bone formation and remodeling at the bone-implant interface in parallel with a novel microscopic analysis. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Caries lesion remineralization with fluoride toothpastes and chlorhexidine - effects of application timing and toothpaste surfactant
Conclusions: The absence of CHX as an adjunct to fluoride toothpastes led to greater remineralization of enamel lesions compared with the immediate use of CHX treatment for both SDS- and CAPB-toothpastes. CAPB-toothpastes indicated significantly greater remineralization than SDS-toothpastes, and can be suggested for patients at high risk of caries. A 30-minute waiting time for CHX treatment is recommended after brushing. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis
This study evaluated the expression of pro-inflammatory (IL-1 β , IL-6, IFN- γ and TNF- α ) and anti-inflammatory (IL-4 and TGF- β ) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. Material and Methods: Apical periodontitis lesions ’ data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels...
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Alterations in the stomatognathic system due to amyotrophic lateral sclerosis
Conclusions: ALS may lead to modifications in the activities of the stomatognathic system, including muscular hyperactivity and reduction in chewing efficiency; however, no change has been observed in the masticatory muscle thickness and molar bite force. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

In-office tooth bleaching with 38% hydrogen peroxide promotes moderate/severe pulp inflammation and production of ll-1β, TNF-β, GPX, FGF-2 and osteocalcin in rats
Abstract Objectives: To study the intensity of inflammatory infiltrate and production of interleukin-1 β (ll-1 β ), tumor necrosis factor- β (TNF- β ), fibroblast growth factor-2 (FGF-2), glutathione peroxidase (GPX), and osteocalcin in response to in-office tooth bleaching in rats. Material and Methods: Twenty male Wistar rats were randomized into four groups (n=5) according to the received treatment (tooth bleaching or no treatment - control) and the period of euthanasia after treatment (24 h or 10 days). We performed tooth bleaching using a 38% hydrogen peroxide gel on maxillary and mandibular inciso...
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

High-concentration carbamide peroxide can reduce the sensitivity caused by in-office tooth bleaching: a single-blinded randomized controlled trial
Conclusions In our study, 37% CP resulted in reduced tooth sensitivity but decreased the tooth bleaching effectiveness. However, both bleaching agents resulted in high levels of patient satisfaction. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Standardization of a protocol for shotgun proteomic analysis of saliva
Conclusions The standardization of protocol for salivary proteomic analysis was satisfactory, since the identification detected typical salivary proteins, among others. The results indicate that using the column for depletion of albumin and IgG is not necessary and that performing individual analysis of saliva samples is possible. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Dental caries and bacterial load in saliva and dental biofilm of type 1 diabetics on continuous subcutaneous insulin infusion
Conclusion Some risk factors related to dental caries were associated with type 1 diabetics. An early diagnosis combined with the evaluation of the risk profile of the diabetic patient is imperative, allowing the dental caries to be analyzed through a perspective of prevention and the patient to be integrated into an individualized oral health program. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice
Conclusions This study showed that anatomical, physiological and morphological abnormalities occur in all three major salivary glands as a natural consequence of aging. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Bioactive glass added to autogenous bone graft in maxillary sinus augmentation: a prospective histomorphometric, immunohistochemical, and bone graft resorption assessment
Conclusions The similarity between the groups in the formation and maintenance of the graft volume after 6 months suggests that the bioactive glass mix ed with autogenous bone (1:1) can be used safely as a bone substitute for the maxillary sinus lift. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - June 11, 2018 Category: Dentistry Source Type: research

Solubility, porosity and fluid uptake of calcium silicate-based cements
Abstract Objective: To evaluate the absorption/fluid uptake, solubility and porosity of White mineral trioxide aggregate (MTA) Angelus, Biodentine (BIO), and zinc oxide-eugenol (ZOE). Material and Methods: Solubility was evaluated after immersion in distilled water for 7 and 30 days. Porosity was evaluated using digital inverted microscope, scanning electron microscope (SEM) and micro-computed tomography (micro-CT). For the fluid uptake test, specimens were immersed in Hank's balanced salt solution (HBSS) for 1, 7, 14 and 28 days. Fluid absorption, solubility and porosity of the materials were measured after each period. S...
Source: Journal of Applied Oral Science - May 21, 2018 Category: Dentistry Source Type: research

Cytidine-phosphate-guanosine oligodeoxynucleotides in combination with CD40 ligand decrease periodontal inflammation and alveolar bone loss in a TLR9-independent manner
Conclusions: These results indicated that CpG plus CD40L decreased periodontal inflammation and alveolar bone loss in a TLR9-independent manner in ligature-induced experimental periodontitis. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - May 21, 2018 Category: Dentistry Source Type: research

Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars
This study analysed the application of vascular endothelial growth factor (VEGF) in the angiogenesis of mature root canals. Material and methods Upper first molars of twelve 13-week old Wistar male rats were used. The root pulp of the mesiobuccal canal was removed and the root canal instrumented with K-files up to size #25. Periapical bleeding was induced into the root canal by introducing a #15 K-file beyond the apex. The teeth on the right side of the arch were filled up with blood clot (G1), whereas those on the left side were filled up with blood clot plus 50 ng/ml of VEGF (G2). Teeth were sealed with light-curing glas...
Source: Journal of Applied Oral Science - May 21, 2018 Category: Dentistry Source Type: research

Assessing the association between hypoxia during craniofacial development and oral clefts
Conclusion Hypoxia is involved in the oral cleft etiology, however, polymorphisms in HIF-1A are not associated with oral clefts in humans. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - May 21, 2018 Category: Dentistry Source Type: research

Osteoradionecrosis of the jaws: case series treated with adjuvant low-level laser therapy and antimicrobial photodynamic therapy
Conclusion: LLLT and aPDT showed positive results as an adjuvant therapy to treat ORNJ. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - May 21, 2018 Category: Dentistry Source Type: research

Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
Conclusions: Regular consumption of milk containing probiotics bacteria reduced CFU/mL of Lactobacillus spp. and increased salivary buffering capacity at 9 months of consumption. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - May 14, 2018 Category: Dentistry Source Type: research

Evaluation of the effect of dose-dependent platelet-rich fibrin membrane on treatment of gingival recession: a randomized, controlled clinical trial
Conclusions: The PRF membrane+CAF technique may be an alternative to the CTG+CAF technique for postoperative patient comfort. However, PRF membranes should use as many layers as possible. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - May 14, 2018 Category: Dentistry Source Type: research

Extracellular calcium increases fibroblast growth factor 2 gene expression via extracellular signal-regulated kinase 1/2 and protein kinase A signaling in mouse dental papilla cells
Conclusions: These findings indicate that elevated extracellular Ca2+ levels led to increased Fgf2 expression through ERK1/2 and PKA in mDP cells and that this mechanism may be useful for designing regenerative therapies for dentin. (Source: Journal of Applied Oral Science)
Source: Journal of Applied Oral Science - May 14, 2018 Category: Dentistry Source Type: research