The frequency of βS-globin haplotypes in the state of Paraná, Brazil, and clinical manifestations of sickle cell anemia
Conclusion: In this study, no association was found between haplotypes and clinical manifestations, probably given the almost absolute predominance of CAR and BEN haplotypes. However, this fact alerts to the possible influence of other polymorphisms and miscegenation in the Brazilian population.RESUMO Introdu ção: A variabilidade nas manifestações clínicas da anemia falciforme (HbSS) pode ser influenciada pelos haplótipos no grupamento da globina β S, nomeados de acordo com a origem geogr áfica: República Centro-Africana (CAR), Benin (BEN), Senegal (SEN) Camar&ot...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 16, 2017 Category: Pathology Source Type: research

The detection of Bence Jones protein in urine by the heat test helps in diagnosis of multiple myeloma?
Conclusion: The evaluation results of BJ protein detection by the heat method sho wed sensitivity of 47.4%, specificity of 91.3%, with positive and negative predictive values of 50% and 90.4%, respectively.RESUMO Introdu ção: O mieloma múltiplo (MM) é uma neoplasia maligna hematológica causada pela intensa proliferação indiscriminada de plasmócitos na medula óssea. Diante da suspeita clínica de MM devem ser realizados exames laboratoriais e exames de imagem, entre outros. Objetivos: Avaliar o teste labora torial de calor para detecção de pr...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 16, 2017 Category: Pathology Source Type: research

Molecular technique for detection and identification of Helicobacter pylori in clinical specimens: a comparison with the classical diagnostic method
Conclusion: PCR is a useful method in the laboratorial routine to detect the presence of H. pylori in the stomach tissue, due to high sensitivity and specificity, but it requires a more careful analysis and standardization.RESUMO Introdu ção: Helicobacter pylori é uma bactéria encontrada nas células epiteliais do trato gastrointestinal humano. Sua infecção relaciona-se com diferentes patologias, como gastrite crônica, úlcera péptica, linfoma gástrico e adenocarcinoma. A infecção por Helicobacter pylori está presente em mais da ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 16, 2017 Category: Pathology Source Type: research

Performance validation of western blot for anti-HIV antibody detection in blood samples collected on filter paper (DBS)
Conclusion: WB optimization allowed the achievement of accurate results, and the easy DBS sample collection, transportation and storage will support the use of this immunodiagnostic technique for conducting HIV/acquired immunodeficiency syndrome (Aids) epidemiological surveys.RESUMO Introdu ção: Em estudos de campo é vantajoso o emprego de amostras coletadas em papel de filtro (DBS) pela simplicidade em manuseá-las e transportá-las. Essas características colocam a tecnologia de DBS em posição de destaque nas pesquisas que buscam aumentar o acesso da populaç&ati...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 16, 2017 Category: Pathology Source Type: research

Molecular methods for detection of Helicobacter pylori infection: could they be the gold standard?
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - March 16, 2017 Category: Pathology Source Type: research

Multisystemic Langerhans cell histiocytosis: case report
This article reports a case of multisystemic Langerhans cell histiocytosis diagnosed by autopsy of a four-day newborn with confirmation by immunohistochemistry and secondary systemic infection associated.RESUMO Histiocitose de c élulas de Langerhans é a nomenclatura designada para um espectro de doenças raras que acomete preferencialmente crianças de 1 a 3 anos, cujo nome refere-se à proliferação de células morfológica e imunofenotipicamente similares às células dendríticas especializadas da pele e da mucosa (cé lulas de Langerhans)....
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma
Conclusion: Probe E showed better rate (67.8%), sensitivity, specificity and accuracy (100%), a very good correlation among the different observers and with PCR, besides great cost-benefits relation.RESUMO Introdu ção: O vírus Epstein-Barr (EBV) pode servir como alvo nos tratamentos terapêuticos, sendo necessário resultado diagnóstico confiável. Objetivo: Avaliar a acurácia da detecção do EBV pela hibridização in situ (ISH), utilizando cinco sondas comerciais em amostras fixadas em formalina e in cluídas em parafina de linfoma de Hodg...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Immunohistochemical analysis of apoptosis and cell proliferation in lungs of premature infants with chronic lung disease (bronchopulmonary dysplasia)
Conclusion: Apoptosis and cell proliferation are involved in the pathophisiology of CLD. The "new" CLD form is more susceptible to apoptosis, while cell proliferation is more evident in the groups with CLD.RESUMO Introdu ção: A fisiopatologia da doença pulmonar crônica, clinicamente conhecida como displasia broncopulmonar (DBP), ainda é incerta. Acredita-se que mecanismos de proteção, como liberação de mediadores inflamatórios e ativação de processos apoptóticos e/ou proliferativos, são acionados no te cido pulmonar d...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Polymethyl methacrylate (Linnea Safe) causes local inflammatory response after intramuscular implant in BALB/c mice but it is not observed in distant organs
Conclusion: The Linnea Safe® PMMA behaved as a safe and stable biomaterial, once its microspheres were sized to prevents phagocytosis, and leads to local and controlled inflammation.RESUMO Introdu ção: Entre suas diversas funções terapêuticas, há mais de uma década o polimetilmetacrilato (PMMA) vem se destacando na reposição de volumes perdidos com processo de envelhecimento e preenchimento de sulcos e rugas. É considerado um biomaterial permanente, apesar de sua confiabilidade ser amplamente discutida por profissinais da área da saúde. ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Daily intake of watercress causes inhibition of experimental Ehrlich tumor growth
Conclusion: The present study demonstrated that the daily intake of aqueous solution of watercress was able to activate a suppression of the EET growth, probably due to the main compounds with antitumor properties present in this vegetable.RESUMO Introdu ção: O aumento da ingestão de vegetais crucíferos tem sido associado à redução de risco de câncer. Estudos experimentais e epidemiológicos sugerem que o agrião é um vegetal crucífero que apresenta alta concentração de compostos com reconhecida atividade antitumoral. Objetivo: I...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Use of the fluid obtained by puncture of cystic hygroma: an alternative method for fetal karyotyping
ABSTRACT The aim of our study aim was to report the case of a fetus with Turner syndrome (TS) diagnosed by karyotype from cystic hygroma (CH) fluid, highlighting the applications and importance of this procedure. First-trimester screening revealed an increased nuchal translucency measurement, cervical cystic hygroma and head and trunk subcutaneous edema. The presence of oligohydramnios prevented the performance of amniocentesis. We performed puncture of the CH for fetal karyotyping, which revealed X-chromosome monosomy (45,X), compatible with TS. Therefore, the use of CH fluid as an alternative sample for fetal karyotyping...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Clinical correlation between the Point-of-care testing method and the traditional clinical laboratory diagnosis in the measure of the lipid profile in patients seen in medical offices
Conclusion: The POCT CardioChek® PA Analyzer is an easy-to-operate tool, with adequate analytical performance and a good correlation with the results of the conventional laboratory method, therefore, considered a reliable me thod.RESUMO Introdu ção: Níveis plasmáticos elevados de lipídios são considerados como principal fator de risco modificável para a doença cardiovascular. A metodologia Point-of-care testing (POCT) fornece resultados rápidos e permite antecipar o diagnóstico e o tratamento. Objetivo: Comparar os resultados do perfil lipídic...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Incidence of factor VIII inhibitory antibodies in patients with hemophilia A seen at HEMOCE, Ceará, Brazil
Conclusion: The high incidence of factor VIII inhibitor in the study population can be explained by the type of treatment used at HEMOCE, which is based on the factor VIII in its recombinant form. The results should be evaluated carefully, so that the treatment and monitoring of these patients are conducted in the safest way possible.RESUMO Introdu ção: A hemofilia A é uma doença hereditária causada pela deficiência do fator VIII, resultante de uma herança genética ligada ao cromossomo X. Durante o tratamento de pacientes com essa doença, pode ocorrer presen&cce...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Analysis of laboratory tests results of patients submitted to a clinical screening program
Conclusion: The main biochemical tests that showed changes, in sequence, were: vitamin D; qualitative urine testing; GPT; HDL-c; triglycerides; blood count; fasting glycemia and urea. The only tests that had the most abnormal rates in young adults, aged 18-39 years, were: parasitological examination of stool; decreased serum levels of urea and glutamic-oxaloacetic transaminase (GOT) and increased values of GPT.RESUMO Introdu ção: Não há trabalhos que demonstrem quais as principais alterações de exames bioquímicos empreendidos em programas de check-up. Objetivo: Identificar o...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Sepsis-associated organ dysfunction and increased supportive care are associated with high serum interleukin-6 levels
Conclusion: These findings suggest that the evaluation of serum IL-6 level is useful in the early phase of the severe sepsis and septic shock in order to identify higher-risk patients.RESUMO Introdu ção: É primordial identificar a sepse e o choque séptico, uma vez que qualquer atraso no início do tratamento adequado associa-se ao pior prognóstico. Objetivo: Avaliar níveis de interleucina 6 (IL-6) no início da evolução da disfunção orgânica na sepse e sua relação com a necessidad e de terapias de suporte avançado. ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Diet and cancer
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - January 13, 2017 Category: Pathology Source Type: research

Aggressive ameloblastic fibrosarcoma in maxilla: case report and new perspectives based on the current literature
ABSTRACT Ameloblastic fibrosarcoma (AFS) is a rare malignant tumor characterized by benign epithelial component within a malignant fibrous stroma. It occurs as a de novo lesion or from a previous ameloblastic fibroma. The aim of this paper is to report an aggressive and recurrent case of AFS in the maxilla of a 38-year-old patient. Histopathological diagnosis can be challenging, especially when it is based on incisional biopsy specimens. Hence, this report highlights not only the importance of the histological features for diagnosis but also the clinical behavior of AFS based on current literature evidence.RESUMO O fibross...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Angiosarcoma of small intestine: case report and literature review
Conclusion: This case demonstrates the difficulty in early diagnosing this pathology, due to its nonspecific clinical presentation. The disease aggressiveness, its unestablished predisposing factors and the controversy around the adequate treatment engender the poor prognosis.RESUMO Introdu ção: Angiossarcomas são patologias raras, e o aparecimento deles no intestino delgado é extremamente incomum. Relato de caso: Paciente do sexo feminino apresentando vômitos, cólica, distensão abdominal e irritação peritoneal. Foram realizadas laparotomia exploratória e...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Preventable causes of death and factors associated with newborn survival at a university hospital in Curitiba, Paraná, Brazil
ABSTRACT Introduction: The analysis of deaths occurred in the neonatal period and the association of these data to necropsy data are crucial to reduce infant mortality rate worldwide. Objective: To analyze the preventable causes of death and the factors associated with a higher risk of early newborn death. Methods: A cross-sectional and descriptive study was performed with data about newborns that died during the neonatal period at a university hospital located in Curitiba; 314 cases of pediatric necropsies were selected, and preventable causes of death, survival time, sex, weight, gestational age, first- and fifth-minute ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Protein molecular modeling of genetic markers for thyroid cancer
Conclusion: This study shows the importance of understanding the molecular structure of the markers for thyroid cancer through bioinformatics, and consequently, the development of more effective new molecules as alternative tools for thyroid cancer treatment.RESUMO Introdu ção: Os avanços nos estudos de biologia molecular da tireoide não fornecem apenas uma visão sobre as doenças tireoidianas, mas um diagnóstico preciso do câncer de tireoide. Objetivo: Realizar um tutorial sobre modelagem molecular proteica dos marcadores genéticos do câncer de tireoide. M...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Histopathological study comparing native and post-transplant recurrent chronic hepatitis C with emphasis on confounders with acute cellular rejection
Conclusion: Histological findings in chronic hepatitis C are similar in non-transplant and post-transplant patients. In addition, morphological features characteristic of ACR are also observed in HCV infection of native livers as well as in the graft of patients with recurrent infection after transplantation.RESUMO Introdu ção: A análise histológica de biópsias hepáticas pós-transplante pode trazer dificuldades na distinção entre hepatite crônica C (HCC) recorrente e outras causas de disfunção do enxerto, sobretudo rejeição cel...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Expression of pathogenicity factors by Enterococcus strains isolated from inpatients with bloodstream infection in Belo Horizonte, Minas Gerais, Brazil
This study adds to knowledge of the pathogenicity profile of Enterococcus strains isolated from BSI, aiming to increase understanding about virulence of the circulating strains in Brazil.RESUMO Neste trabalho, avaliou-se o perfil de patogenicidade de 35 amostras de Enterococcus isoladas de pacientes com infec ção de corrente sanguínea (ICS) em diferentes hospitais de Belo Horizonte (MG), Brasil. E. faecalis foi a espécie mais prevalente (27/77,14%). A produção de gelatinase e citolisina, detectada em 14/40% das amostras, apresentou distribuição heterogênea. A pr...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Report of a case of paroxysmal nocturnal hemoglobinuria (PNH) with complex evolution and liver transplant
We report the case of a 32 year-old female PNH patient with bone marrow aplasia, which followed a complex course, diagnosed with aplastic anemia associated with PNH, evolving in three years with Budd-Chiari syndrome and liver transplantation. Post-transplant complications, hepatic arterial thrombosis, graft rejection, liver retransplantation and treatment of PNH with eculizumab. Clinical stabilization and cessation of symptoms were achieved.RESUMO Hemoglobin úria paroxística noturna (HPN) é uma doença rara, adquirida, com episódios trombóticos e pancitopenia frequente. Relatamos o ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Hemoglobinopathy detection through an institutional neonatal screening program in Colombia
Discussion and conclusion: Taking in consideration data from the World Health Organization (WHO) on hemoglobinopathies, our prevalence of> 1% is considered high. Therefore, a more extended coverage and the need for a national screening program are priorities.RESUMO Introdu ção: As hemoglobinopatias são doenças genéticas comuns em todo o mundo e representam um problema de saúde pública. Na Colômbia, embora existam áreas geográficas com maior risco de apresentá-las, não há programas de triagem nem estudos para estabelecer sua preval&...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Biofilm production and resistance profile of Enterobacter sp. strains isolated from pressure ulcers in Petrolina, Pernambuco, Brazil
Conclusion: The strains of Enterobacter sp. showed high resistance to the antimicrobials used in clinical routine.RESUMO Introdu ção: O gênero Enterobacter é formado por bactérias fermentadoras de lactose. Esse microrganismo causa largo espectro de infecções hospitalares, como pneumonia, infecções do trato urinário e feridas, além de associar-se à colonização de dispositivos hospitalares. Objetivo: Definir o pe rfil de resistência e a produção de biofilme de cepas de Enterobacter sp. isoladas de úlcer...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Detection of antimicrobial resistance genes in beta-lactamase- and carbapenemase-producing Klebsiella pneumoniae by patient surveillance cultures at an intensive care unit in Rio de Janeiro, Brazil
Conclusion: Although no clonal relationship was observed within the isolates, this study revealed alarming data on the antimicrobial resistance deficiently monitored for preventive purposes in Brazil. Our data allow us to conclude that the inclusion of surveillance cultures in health facilities is a recommended strategy aiming particularly at preventing the spread of resistance genes in the hospital environment and, consequently, reducing morbidity and mortality.RESUMO Introdu ção: O aumento da incidência de microrganismos multirresistentes é considerado um dos principais problemas de saúd...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Bacterial resistance in enterobacteria
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - December 9, 2016 Category: Pathology Source Type: research

Adenocarcinoma of the female urethra: a case report
ABSTRACT The urethral adenocarcinoma is a rare malignant tumor that affects predominantly women. Its incidence is higher after the age of 50 in this group. The knowledge about this disease is limited due to its low frequency and is mainly based on case reports. Although rarely presenting distant metastasis, the clinical consequences of treating this condition may bring an important negative impact on patient's quality of life. This is mainly due to mutilating surgery and the need to use permanent urinary catheter. It is presented a case report of a patient with urethral adenocarcinoma and its therapeutic management.RESUMO ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Invasive bladder urothelial carcinoma, plasmacytoid variant: case report
We report a case of a 79 years old male, subjected to transurethral resection of bladder tumor, which histological examination revealed a pT1 high-grade urothelial carcinoma. Subsequently, he underwent radical cystoprostatectomy, which showed urothelial carcinoma with lack of cohesion, plasmacytoid variant, positive for citokeratin 7 (CK7), citokeratin 20 (CK20) and trans-acting T-cell-specific transcription factor (GATA-3), and negative for E-cadherin and CD138. It is important to recognize the plasmacytoid variant of the invasive urothelial carcinoma, since it avoids a potential misdiagnosis of metastatic cancer.RESUMO C...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Quantitative analysis of inflammatory and adhesion molecules in lungs of neonates with chronic lung disease (bronchopulmonary dysplasia) receiving mechanical ventilation
ABSTRACT INTRODUCTION: Chronic lung disease (CLD), clinically known as bronchopulmonary dysplasia (BPD), is a major cause of morbidity in premature newborn and were submitted to oxygen therapy. OBJECTIVE: Immunohistochemical identification of inflammatory molecules in the lung tissue of premature neonates that died with CLD. METHODS: Immunohistochemical analysis of 51 samples of premature newborn lungs – grouped in: without CLD,"classic" CLD and"new" CLD. RESULTS: Neutrophil influx and the number of CD4+ and CD45RO+ cells were higher in the"classic" CLD group (p
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Features of the female prostate according to age: an autopsy study
CONCLUSION: This study suggests that the number of female prostate glands increases after menopause, with proliferative spread and growth of the median portion to the proximal and distal portions.RESUMO INTRODU ÇÃO: A próstata é uma glândula com papel importante no sistema reprodutor masculino e feminino. Dados sobre esse órgão não foram completamente explorados em mulheres desde a sua primeira descrição, provavelmente por ser considerada uma glândula vestigial. OBJETIVO: Correlacionar a morfol ogia da próstata feminina com a idade em aut&oac...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Cervical cancer screening: from Pap smear to future strategies
ABSTRACT Previously, the screening for detection of cervical cancer was performed by simple cervicovaginal sample collected by the physician whenever the patient attended the medical consultation, and soon it was established as the annual"Pap smear". Since then, an elementary test has evolved into a complex process with multiple algorithms for the identification of invasive disease. The detection of human papillomavirus (HPV) has become part of the new screening recommendations, resulting in major changes in the guidelines. This review intends to emphasize the most important topics that are part of cervical cance...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Species distribution and antifungal susceptibility to vulvovaginal Candida spp. in southern Mato Grosso State, Brazil
CONCLUSION: C. albicans was the predominant species. We observed a high susceptibility of Candida spp. to fluconazole and ketoconazole antifungal.RESUMO INTRODU ÇÃO: A candidíase vulvovaginal é uma prevalente infeccção mucosa oportunista, causada predominantemente por Candida albicans. As espéceis de Candida variam de acordo com a suscetibilidade aos agentes antifúngicos, assim os testes de sensibilidade têm importância clínica para uma adequada escolha terapêutica. OBJETIVO: Investigar a distribuição de leveduras vulvovaginais e...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Prevalence of factor V Leiden in patients with venous thrombosis
CONCLUSION: In conclusion, the research of mutation on factor V has strong impact on investigation of venous thromboembolism, in order to elucidate the etiology of the event, and in treatment and in prophylaxis against the recurrence.RESUMO INTRODU ÇÃO: A trombose venosa é uma doença de caráter multifatorial e de alta incidência na população. O desenvolvimento dessa patologia está intimamente ligado à presença de fatores ambientais e genéticos, podendo ocorrer em associação ou isoladamente. Entre as diversas mutaçõ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Is the creatine kinase isoenzyme MB level a marker of myocardial ischemia in ventilated premature infants?
CONCLUSION: The increase of CK-MB serum levels in VG and SG seems to indicate an increased work of thoracic skeletal muscle and do not represent a MI signal, which invalidate its use as a marker.RESUMO INTRODU ÇÃO: Não está claro se os níveis séricos de troponina I (TI) e de creatinoquinase fração MB (CK-MB) estão alterados em recém-nascidos prematuros (RNP) sem indícios de isquemia miocárdica (IM). OBJETIVO: Investigar se os níveis de TI e CK-MB se alteram nos RNP sem IM quando em ventilaç ão mecânica (VM). M&Eac...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Application of PCR-SSP method for HLA-B*27 identification as an auxiliary tool for diagnosis of ankylosing spondylitis
CONCLUSION: Considering the high reproducibility and the broad spectrum of subtypes covering, it was concluded that the PCR-SSP identifying method for HLA-B*27 can be used as a routine diagnostic tool f or spondyloarthropathies.RESUMO INTRODU ÇÃO: Os antígenos leucocitários humanos (HLA) são moléculas que apresentam antígenos ao sistema imune; a presença ou a ausência deles é descrita como fator influente em algumas doenças. O HLA-B*27 é um polimorfismo do HLA que tem sido associado à maior predisposição à espondi...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Association between the glutathione S-transferase P1 (GSTP1) Ile105Val gene polymorphism in obese and overweight patients over 60 years
This study aimed to analyze the frequency of the alleles (A and G) and the genotypes of the GSTP1 Ile105Val gene polymorphism, and its association with obesity in the elderly. MATERIALS AND METHODS: This was a cross-sectional study involving 232 subjects aged between 60-98 years, of both sexes, originating from southern Brazil. The volunteers were categorized according to the body mass index (BMI) in three groups: normal weight (n = 52), overweight (n = 133), and obese (n = 47). Anthropometry was evaluated and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for genetic anal...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Review of the Brazilian Guidelines for Cervical Cancer Screening, 2016
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - September 13, 2016 Category: Pathology Source Type: research

Mucocutaneous Kaposi's sarcoma in an HIV-positive patient: diagnosis and treatment
ABSTRACT Kaposi's sarcoma (KS) is an endothelial neoplasia caused by infection with the human herpesvirus 8 (HHV-8), and the type associated with the human immunodeficiency virus (HIV) is considered the most aggressive and frequent. This paper reports a case of mucocutaneous KS in a patient not formerly aware of being an HIV bearer. A 38-year-old male patient has sought treatment with multiple oral lesions and one in the skin. Serology was positive for HIV and incisional biopsy diagnosed KS. After 11 months of chemotherapy and antiretroviral therapy, there was complete remission of the skin KS and partial remission of oral...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Intraoral spindle-cell lipoma with chondroid differentiation: importance in the diagnosis of oral lesions presenting chondroid tissue
We present a case of a 62-year-old woman with a submucous nodule located in the tongue. Histopathological examination revealed an encapsulated tumor composed of myxoid tissue, spindle cells and mature adipocytes in transition to cartilaginous tissue. The final diagnosis was spindle-cell lipoma with myxoid change and chondroid differentiation. No sign of recurrence was found after five years. The diagnosis of intraoral mesenchymal lesions with chondroid differentiation requires careful histologic examination, mainly to differentiate between benign and malignant lesions.RESUMO Lipomas s ão neoplasias benignas de tecid...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Bone marrow necrosis: literature review
Conclusion: The search for an underlying associated malignancy is important for the management of BMN.RESUMO Introdu ção: Necrose de medula óssea (NMO) é uma entidade rara, frequentemente não diagnosticada e mais comumente associada a doenças hematológicas. Metodologia: Realizou-se revisão da literatura na base de dados do PubMed, utilizando o termo "necrose de medula óssea". Foram encontrados 25 artigos em inglês e 65 relatos de caso. Resultados e discussão: A fisiopatologia da NMO não é bem elucidada e parece estar associa...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Evaluation of the use of laboratory tests in primary care
Conclusion: Knowing and monitoring laboratory attention enables health unit managers to enhance effectiveness and rational use of the scarce available resources.RESUMO Introdu ção: A avaliação da utilização dos exames laboratoriais na atenção primária pode melhorar a qualidade do atendimento prestado à população. Objetivo: Avaliar a utilização de exames laboratoriais pelas unidades de atenção primária à saúde de Botucatu, São Paulo, Brasil. Métodos: Foram realizados grupos tutor...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Kinetics of the expressions of radiation-induced plasma proteins of the cardiac territory in electrophoresis
Conclusion: These findings point out to acute alterations of protein expression modulated in time, but also to a late modulation of gene expression.RESUMO Introdu ção: A radiação de corpo inteiro induz alterações agudas e tardias no perfil proteico sanguíneo. Objetivo: O presente estudo tem como escopo analisar em cinética de tempo o perfil eletroforético de bandas de expressão das proteínas solúveis do sangue, na faixa de peso molecular superior e igual à albumina, moduladas por radiação ionizante no território ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Clindamycin microbial resistance in clinical isolates of Staphylococcus sp. derived from blood cultures of hospitalized patients
Conclusion: The D-test is a key test for the constant monitoring of the inducible resistance phenotype (which may impair the effectiveness of treatment with clindamycin), minimizing potential medication errors and adverse clinical outcomes.RESUMO Introdu ção: As infecções causadas por cepas de Staphylococcus aureus resistente à meticilina (MRSA) tornaram-se comuns no âmbito hospitalar, e essa resistência limitou o tratamento dos pacientes com antibióticos betalactâmicos. A clindamicina é um importante agente terapêutico para as infecçõ e...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Metrological traceability in clinical laboratory
This study shows the availability of information on metrological traceability provided by diagnostic kit manufacturers. Method: It was done with the information obtained in kits of glucose, urea, creatinine, uric acid and cholesterol participants in a proficiency testing program throughout 2012 and 2013. Results and conclusion: In the research, 18 producers of reference materials (RMs) and 204 measurands available for laboratory medicine were found. In the study of metrological traceability, the preferential use of CRM was demonstrated by kit manufacturers (glucose 60%, urea 69%, creatinine 60%, uric acid 50%, and choleste...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Implementation of criteria for automatic release of clinical chemistry test results in a laboratory at an academic public hospital
Conclusion: Autoverification implementation led to a more consistent reviewing process of test results, efficiency and improved patient safety.RESUMO Introdu ção: A verificação automática dos resultados consiste na liberação dos resultados dos exames diretamente do equipamento, sem intervenção humana. São inseridas regras para avaliar os resultados no middleware e/ou no sistema de informação laboratorial (SIL), com base em critérios estabele cidos pelo laboratório. A autoverificação uniformiza os critérios...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange
Conclusion: Results were analyzed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) to assess interchangeability between the methods, by linear regression analysis and paired t-test. The exclusion of interfering fragments from result analysis by the modified method produced results in closer proximity to those of the reference method.RESUMO Introdu ção: A contagem de reticulócitos por citometria de fluxo (CF) - um método de contagem automatizada - pode apresentar erros devido à presença de interferentes, contribuindo para uma ligeira eleva&c...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Anti-alpha-fodrin antibodies in patients with Sjögren's syndrome secondary to rheumatoid arthritis
ABSTRACT Introduction: The Sj ögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration. The currently most researched antibodies for its diagnosis are anti-La and anti-Ro, which, however, have low specificity in the case of SS secondary to rheumatoid arthritis (RA) and systemic lupus erythematosu s (SLE). The antibodies against alpha-fodrin (AF) have been proposed to diagnose SS. Objective: In the present study, we investigated the anti-AF antibody in a group of RA patients with and without secondary SS (sSS). Methods: Were studied 90 consecutive patients with RA (48.8% of them with SS)...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Platelet interference in automated reticulocyte counting
(Source: Jornal Brasileiro de Patologia e Medicina Laboratorial)
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - August 2, 2016 Category: Pathology Source Type: research

Proliferating trichilemmal tumor: case report
ABSTRACT The proliferating trichilemmal tumor is a lesion with trichilemmal differentiation, more common occurring among elderly women; it affects the scalp with sizes ranging from 2-10 cm. Microscopically, it is solid-cystic, well-defined, affecting the dermis and the subcutaneous cellular tissue. It presents trichilemmal and squamous keratinization. Pleomorphism may be present. Ghosts, apocrine and spindle cells can be observed. The differential diagnosis should be performed with malignant trichilemmal tumor and squamous cell carcinoma. Their behavior is benign and complete resection is recommended. Our goal is to report...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - May 18, 2016 Category: Pathology Source Type: research