Microbial cell autofluorescence as a method for measuring the intracellular content of B2 and B6 vitamins
In this report, we demonstrate a simple, rapid, and sufficiently trustworthy spectrofluorimetric method for determining the content of vitamins B2 and B6 in yeast cells which consists of cells growing, harvesting, washing, and resuspending in a buffer, and then measuring the emitted visible light using specific wavelength of excitation (λex=340 nm and λem=385 nm for pyridoxine; λex=460 nm and λem=535 nm for riboflavin). The limits of detection (LOD) and quantification (LOQ) estimated through measurements of vitamin fluorescence were below 0.005 μg/ml for riboflavin and below 0.05 μg/ml for pyridoxine, respectively. I...
Source: International Journal for Vitamin and Nutrition Research - October 20, 2023 Category: Nutrition Authors: Roman Maslanka Micha ł Przywara Agnieszka Janeczko Renata Zadrag-Tecza Source Type: research

The anti-infective effect of β-glucans in children
Conclusions: Good tolerance of β-glucans shows a favorable benefit-risk ratio of this type of intervention. Nevertheless, further monitoring of their efficacy and safety in high-quality research is necessary.PMID:37779363 | DOI:10.1024/0300-9831/a000793 (Source: International Journal for Vitamin and Nutrition Research)
Source: International Journal for Vitamin and Nutrition Research - October 2, 2023 Category: Nutrition Authors: Katarzyna Wzorek- Łyczko Weronika Wo źniak Anna Piwowarczyk Ernest Kuchar Source Type: research