Building a reference indicator model using co ‐kriging interpolation to determine the geographical origin of the flighted spongy moth complex in China
In this study, we representatively sampled the Chinese provinces where flighted spongy moth complex (FSMC) has been reported and, for the first time, used co-kriging interpolation to predict the distribution patterns of FSMCδ13C values in the main distribution areas. From 2020 to 2022, we set up 60 traps in 12 provinces and cities in China and collected 795 FSMCs. Then, 6 main climatic factors were obtained by multi-collinearity screening from 21 types of meteorological data collected at the sample plots, and a correlation analysis was carried out by combining longitude, latitude, and altitude data with theδ13C values of...
Source: Insect Science - February 23, 2024 Category: Biology Authors: Zeshi Qin, Yixia Cao, Yan Wang, Jun Ding, Wujia Xia, Juan Shi Tags: ORIGINAL ARTICLE Source Type: research

AsOBP1 is required for bioallethrin repellency in the malaria vector mosquito Anopheles sinensis
Bioallethrin, the first commercially available volatile pyrethroid, has been used worldwide in insecticides and repellents. The olfactory mechanism of the repellency elicited by bioallethrin in mosquitoes is still unclear. The binding ability of recombinantAsOBP1 protein to bioallethrin and the reduced bioallethrin repellency and electroantennogram responses inAsOBP1−/− mutants demonstrated that theAsOBP1-mediated olfactory pathway is an important component of bioallethrin repellency inAnopheles sinensis. Moreover, the reduced bioallethrin repellency in pyrethroid-resistant mosquitoes may be related tokdr mutation and ...
Source: Insect Science - February 23, 2024 Category: Biology Authors: Yongjie Zhang, Shulin He, Chengyin He, Ling Zhou, Ou Xu, Liang Qiao, Bin Chen, Yueqing Cao, Zhengbo He Tags: ORIGINAL ARTICLE Source Type: research

The symbiont Wolbachia alleviates pesticide susceptibility in the two ‐spotted spider mite Tetranychus urticae through enhanced host detoxification pathways
Wolbachia infection increases pesticide resistance in spider miteTetranychus urticae.Wolbachia infection upregulates the expression of detoxification genes inT. urticae. Abamectin treatment significantly improvedWolbachia abundance inT. urticae. AbstractThe two-spotted spider mite (Tetranychus urticae) is one of the most well-known pesticide-resistant agricultural pests, with resistance often attributed to changes such as target-site mutations and detoxification activation. Recent studies show that pesticide resistance can also be influenced by symbionts, but their involvement in this process in spider mites remains uncert...
Source: Insect Science - February 23, 2024 Category: Biology Authors: Qing ‐Tong Ye, Xue Gong, Huan‐Huan Liu, Bing‐Xuan Wu, Chang‐Wu Peng, Xiao‐Yue Hong, Xiao‐Li Bing Tags: ORIGINAL ARTICLE Source Type: research

Microbial changes and associated metabolic responses modify host plant adaptation in Stephanitis nashi
This study demonstrated changes in microbial diversity and the reshaping of microbial community structure in the pear lace bugStephanitis nashi when it fed on 2 different host plants, and showed howS.  nashi mobilized a series of metabolic reactions to adapt to these host plants. Association analysis further clarified the one-to-one correspondence between differential bacteria, differential fungi, and functional responses in this process, which revealed the mechanisms ofS.  nashi adaptation to 2 different host plants from a microbiological perspective. AbstractSymbiotic microorganisms are essential for the physiological ...
Source: Insect Science - February 19, 2024 Category: Biology Authors: Tong ‐Pu Li, Chen‐Hao Wang, Jia‐Chu Xie, Meng‐Ke Wang, Jie Chen, Yu‐Xi Zhu, De‐Jun Hao, Xiao‐Yue Hong Tags: ORIGINAL ARTICLE Source Type: research

Role of aminopeptidase N ‐like in the acquisition of begomoviruses by Bemisia tabaci, the whitefly vector
In this study, we identified an aminopeptidase N-like protein (BtAPN) inB.  tabaci Asia  II 1, an efficient vector of SLCMV, which is involved in the SLCMV transmission process. Through the use of glutathione S-transferase pull-down assay and LC-MS/MS analysis, we demonstrated the interaction between BtAPN and the coat protein (CP) of SLCMV. This interaction was further confirmedin vitro, and we observed an induction ofBtAPN gene expression following SLCMV infection. By interfering with the function of BtAPN, the quantities of SLCMV were significantly reduced in various parts ofB.  tabaci Asia  II 1, including the wh...
Source: Insect Science - February 19, 2024 Category: Biology Authors: Yun ‐Yun Fan, Yao Chi, Na Chen, Wilmer J. Cuellar, Xiao‐Wei Wang Tags: ORIGINAL ARTICLE Source Type: research

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AbstractAs a natural antiviral defense system, the RNAi machinery is activated by viral dsRNA signatures, resulting in the targeted degradation of viral genomic RNAs and transcripts. Exogenously delivered insecticidal dsRNA also trigger RNAi via the same RNAi machinery, but in this case the target is the transcript of a gene essential for development or survival. As we learn more about the natural role of RNAi in viral immunity, the underlying mechanisms, and the factors that influence RNAi efficacy against agricultural pests, these studies will support the implementation of more RNAi-based insect pest control strategies, ...
Source: Insect Science - February 13, 2024 Category: Biology Tags: CONTENTS Source Type: research

Issue Information
(Source: Insect Science)
Source: Insect Science - February 13, 2024 Category: Biology Tags: ISSUE INFORMATION Source Type: research

Correction
(Source: Insect Science)
Source: Insect Science - February 13, 2024 Category: Biology Tags: CORRECTION Source Type: research

Characterization of two  Bursicon genes and their association with wing development in the brown citrus aphid, Aphis citricidus
In this study, we successfully identified theAcBurs- α andAcBurs- β genes inAphis citricidus. The open reading frames ofAcBurs- α andAcBurs- β were 480 and 417  bp in length, respectively. Both AcBurs-α and AcBurs-β exhibited 11 conserved cysteine residues.AcBurs- α andAcBurs- β were expressed during all developmental stages ofA. citricidus and showed high expression levels in the winged aphids. To investigate the potential role ofAcBurs- α andAcBurs- β in wing development, we employed RNA interference (RNAi) techniques. With the efficient silencing ofAcBurs- α (44.90%) andAcBurs- β (52.31%), malformed wings w...
Source: Insect Science - February 10, 2024 Category: Biology Authors: Jin ‐Ming Lu, Feng Shang, Bi‐Yue Ding, Lin Wang, Qing‐Chun Li, Jin‐Jun Wang, Wei Dou Tags: ORIGINAL ARTICLE Source Type: research

Construction of cytomegalovirus promoter ‐driven gene expression system in Laodelphax striatellus
This study aimed to construct an expression vector using the promoter of the constitutive-expressedtubulin gene of SBPH, and promoter of human cytomegalovirus (CMV). Fluorescence experiments demonstrated that bothtubulin and CMV promoter could drive green fluorescent protein (GFP) expression in SBPH, and could also facilitate the expression of a nucleocapsid protein (NP) -GFP fusion protein containing viral NP with comparable efficiency. Through expression vector optimization, we have identified that the 3 tandem CMV promoters display a significantly higher promoter activity compared with both the 2 tandem CMV promoters an...
Source: Insect Science - February 10, 2024 Category: Biology Authors: Xiaohui Cheng, Wan Zhao, Guohua Liang, Hong Lu, Yumei Fu, Yiming Li, Feng Cui Tags: ORIGINAL ARTICLE Source Type: research

High ‐throughput molecular gut content analysis of aphids identifies plants relevant for potato virus Y epidemiology
This study provides the first evidence from high-throughput molecular GCA of aphids and reveals host use patterns that are relevant for PVY epidemiology. (Source: Insect Science)
Source: Insect Science - February 7, 2024 Category: Biology Authors: William Jacob Pitt, William Rodney Cooper, Derek Pouchnik, Heather Headrick, Punya Nachappa Tags: ORIGINAL ARTICLE Source Type: research

m ‐Aminophenylacetylene induces maternal care in a predatory spider
In this study, we investigated maternal care inPardosa pseudoannulata (Araneae, Lycosidae) females. We proposed that the physical interaction between pulli and mother spiders induces maternal care viam-aminophenylacetylene (m-A), a novel regulator of maternal care. The presence of pulli on the dorsal abdomen of non-mother spiders suppressed pulli predation and increased the pulli-carrying rate, and the absence of pulli on the mother spiders increased pulli predation and decreased the pulli-carrying rate. The compoundm-A was abundant in mother spiders, and it could be induced in non-mother spiders when they carried pulli. T...
Source: Insect Science - February 5, 2024 Category: Biology Authors: Yunru Chen, Na Yu, Shuchen Dong, Guanqun Li, Huiya Pan, Zonglei Guo, Zewen Liu Tags: ORIGINAL ARTICLE Source Type: research

Insulin receptor participates in the peripheral olfactory processes of honey bees (Apis cerana cerana)
In conclusion, our findings provide a foundation for understanding the involvement of AcerInR in the odor perception ofA.  cerana cerana. Moreover, they offer novel insights into the olfactory recognition mechanism in insects. (Source: Insect Science)
Source: Insect Science - February 2, 2024 Category: Biology Authors: Huiting Zhao, Longlong Chen, Miaomiao Liu, Shuguo Zhao, Weihua Ma, Yusuo Jiang Tags: ORIGINAL ARTICLE Source Type: research

Conservation genomics analysis reveals recent population decline and possible causes in bumblebee Bombus opulentus
Conservation genomics methods were used to understand the recent demography ofBombus opulentus populations and results indicated that one of the bumblebee's populations had experienced a long-term decline, which should be caused by a combination of high-temperature, microsporidia infection, and pesticides exposure. AbstractBumblebees are a genus of pollinators (Bombus) that play important roles in natural ecosystem and agricultural production. Several bumblebee species have been recorded as under population decline, and the proportion of species experiencing population decline within subgenusThoracobombus is higher than av...
Source: Insect Science - February 1, 2024 Category: Biology Authors: Huiling Sang, Yancan Li, Shuxin Tan, Pu Gao, Bei Wang, Shengnan Guo, Shudong Luo, Cheng Sun Tags: ORIGINAL ARTICLE Source Type: research

BmSLC7A5 is essential for silk protein synthesis and larval development in Bombyx mori
We demonstrate that amino acid signaling regulates silk protein synthesis and larval development by L-type amino acid transporter  1 inBombyx mori. Feed intake is increased in the early 5th instar, which accelerates nutrient absorption in the midgut and accumulation of amino acids in the hemolymph. Meanwhile, the expression ofBmSLC7A5 shows an enhancement, which is positively correlated with leucine content in the silk gland. Leucine increases the activity of TORC1 signaling, and 4EBP1 and S6K1 are phosphorylated by TORC1, which activate eukaryotic translation initiation factors and ribosomal protein S6 to improve protein...
Source: Insect Science - January 30, 2024 Category: Biology Authors: Xin Tang, Huawei Liu, Xin Wang, Li Chang, Qingsong Liu, Qingyou Xia, Ping Zhao Tags: ORIGINAL ARTICLE Source Type: research