MicroR ‐9c‐5p and novel‐mir50 co‐target Akt to regulate Lasioderma serricorne reproduction
This study identified 2 binding sites ofmiR-9c-5p andnovel-mir50 in the coding sequences ofLsAkt. The expression profiles of 2 microRNAs (miRNAs) andLsAkt displayed an opposite pattern during the adult stages. Luciferase reporter assay showed thatnovel-mir50 andmiR-9c-5p could downregulate the expression ofLsAkt. Overexpression ofmiR-9c-5p andnovel-mir50 by injection of mimics inhibited the expression ofLsAkt and reduced oviposition, decreased egg hatchability, and blocked ovarian development. It also decreased the expression of genes involved in ovarian development (LsVg andLsVgR) and the nutritional signaling pathway (Ls...
Source: Insect Science - June 23, 2023 Category: Biology Authors: Yi Yan,
Dong ‐Dong Qin,
Hong Yang,
Kang‐Kang Xu,
Can Li,
Wen‐Jia Yang Tags: ORIGINAL ARTICLE Source Type: research
The evolution and functional divergence of 10 Apolipoprotein D ‐like genes in Nilaparvata lugens
In this study, we identified 10ApoD-like genes (NlApoD1 −10) with distinct spatiotemporal expression patterns inNilaparvata lugens (BPH), which is an important pest of rice.NlApoD1 −10 were found to be distributed on 3 chromosomes in a tandem array ofNlApoD1/2,NlApoD3 −5, andNlApoD7/8, and show sequence and gene structural divergence in the coding regions, indicating that multiple gene duplication events occurred during evolution. Phylogenetic analysis revealed that NlApoD1 −10 can be clustered into 5 clades, with NlApoD3−5 and NlApoD7/8 potentially evolving exclusively in the Delphacidae family. Functional scree...
Source: Insect Science - June 20, 2023 Category: Biology Authors: Jia ‐Bao Lu,
Peng‐Peng Ren,
Qiao Li,
Fang He,
Zhong‐Tian Xu,
Sai‐Nan Wang,
Jian‐Ping Chen,
Jun‐Min Li,
Chuan‐Xi Zhang Tags: ORIGINAL ARTICLE Source Type: research
Hexamerin and allergen are required for female reproduction in the American cockroach, Periplaneta americana
Two proteins, hexamerin and allergen, separated from the fat body of female adults of the American cockroach, were identified as storage proteins. They play an important role in the juvenile hormone-stimulated female reproduction and may be involvedvia metabolism or nutrition. AbstractReproduction is of great importance for the continuation of the species. In insects, the fat body is the major tissue for nutrient storage and involved in vitellogenesis, which is essential for female reproduction. Here, 2 proteins, hexamerin and allergen, were separated from the fat bodies of adult female American cockroaches (Periplaneta am...
Source: Insect Science - June 17, 2023 Category: Biology Authors: Shiming Zhu,
Xiaoyi Chen,
Sishi Xia,
Qin Li,
Ziqi Ye,
Shaoting Zhao,
Kexin Liu,
Fangfang Liu Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: Insect Science)
Source: Insect Science - June 14, 2023 Category: Biology Tags: ISSUE INFORMATION Source Type: research
Cover Caption
The Glanville fritillary butterfly,Melitaea cinxia, is widely distributed between approximately 35 °N and 60°N in Eurasia, from the Atlantic coast to the Baikal Lake region. In China,M. cinxia is distributed in Xinjiang. The geographic populations have to face greatly different ambient temperatures. The biochemical adaptation strategy ofM. cinxia is revealed in Chinese population for dealing with high temperature (see pages 758-770). Photo provided by Rongjiang Wang. (Source: Insect Science)
Source: Insect Science - June 13, 2023 Category: Biology Tags: CONTENTS Source Type: research
Gene cloning, protein expression, and enzymatic characterization of a double ‐stranded RNA degrading enzyme in Apolygus lucorum
Characterization of enzymatic activity of heterologously expressed AldsRNase at different pH, incubation time and temperature. The more acidic intestinal (pH 5) environment inApolygus lucorum has promoted the activity of the recombinant protein AldsRNase enzyme as reflected by short degradation time (1 min) at 30 °C. AbstractRNA interference (RNAi) is a powerful tool that post-transcriptionally silences target genes in eukaryotic cells. However, silencing efficacy varies greatly among different insect species. Recently, we met with little success when attempting to knock down genes in the mirid bugApolygus lucorum via ...
Source: Insect Science - June 8, 2023 Category: Biology Authors: Jie ‐Yu Zhang,
Jing Zhao,
Keyan Zhu‐Salzman,
Qin‐Qin Ji,
Yi‐Ping Jiang,
Liu‐Bin Xiao,
De‐Jin Xu,
Guang‐Chun Xu,
Lin‐Quan Ge,
Yong‐An Tan Tags: ORIGINAL ARTICLE Source Type: research
The genome sequence of a spider mite, Tetranychus truncatus, provides insights into interspecific host range variation and the genetic basis of adaptation to a low ‐quality host plant
The phytophagous miteTetranychus truncatus is a significant pest in East Asia, but its host range is relatively narrower than that ofTetranychus urticae, another pest mite. We have produced a high-quality, chromosomal-level genome ofT. truncatus and compared it with that ofT. urticae. Our findings indicate thatT. truncatus has fewer genes associated with detoxification, transport, and chemoreception thanT. urticae, with a notable reduction in gustatory receptor genes. Through population genetics analyses and comparisons of transcriptomes after a host plant shift, we have identified genes that are potentially involv...
Source: Insect Science - June 7, 2023 Category: Biology Authors: Lei Chen,
Xin ‐Yue Yu,
Xiao‐Feng Xue,
Feng Zhang,
Li‐Xue Guo,
Hua‐Meng Zhang,
Ary A. Hoffmann,
Xiao‐Yue Hong,
Jing‐Tao Sun Tags: ORIGINAL ARTICLE Source Type: research
Higher levels of the lipophilic antioxidants coenzyme Q10 and vitamin E in long ‐lived termite queens than in short‐lived workers
This study provides essential biochemical and evolutionary insights into the relationship between CoQ10 concentrations and termite lifespan extension. (Source: Insect Science)
Source: Insect Science - June 6, 2023 Category: Biology Authors: Eisuke Tasaki,
Yorihiro Yamamoto,
Yoshihito Iuchi Tags: ORIGINAL ARTICLE Source Type: research
Characterization of the pheromone receptors in Mythimna loreyi reveals the differentiation of sex pheromone recognition in Mythimna species
Molecular recognition mechanism of sex pheromones and analogs was revealed inMythimna loreyi by analyzing the antennal transcriptome. MlorPR6 and MlorPR3 were the receptors of major and minor components. MlorPR1 and MlorPR5 both possessed the ability to detect pheromones of sympatric species. AbstractPheromone receptors (PRs) are key proteins in the molecular mechanism of pheromone recognition, and exploring the functional differentiation of PRs between closely related species helps to understand the evolution of moth mating systems. Pheromone components of the agricultural pestMythimna loreyi have turned into (Z)-9-tetrad...
Source: Insect Science - June 3, 2023 Category: Biology Authors: Chan Wang,
Lei Liu,
Tian ‐Yu Huang,
Yu Zhang,
Yang Liu,
Gui‐Rong Wang Tags: ORIGINAL ARTICLE Source Type: research
Chromosome ‐level genome assembly of the Asian citrus psyllid, Diaphorina citri
We have assembled one of the highest quality chromosome-levelDiaphorina citri genomes to date and found by resequencing that theD. citri sex determination system is XO. Based on the phylogenetic and genomic comparison,D.citri andPachypsylla venusta were found to be most closely related and separated from their most recent common ancestor about 336.62 million years ago. Genes potentially involved in detoxification metabolism, pathogen transmission, and honeydew secretion were screened. AbstractDiaphorina citri is a global citrus pest. As a vector insect, it can transmit the causative agents of citrus huanglongbing, causin...
Source: Insect Science - May 26, 2023 Category: Biology Authors: Shuang Lei,
Shi ‐Jiang Yu,
Qi Pan,
Li‐Li Ding,
Si‐Chen Li,
Lu‐Yan Cheng,
Shu‐Qi Wang,
Bing‐Hai Lou,
Jun He,
Cui‐Yun Lei,
Lin Cong,
Hao‐Qiang Liu,
Xue‐Feng Wang,
Chun Ran Tags: ORIGINAL ARTICLE Source Type: research
Experimental arena settings might lead to misinterpretation of movement properties
We examined the effect of the arena structure on movement properties using the red flour beetle as a model. The arena structure greatly affected movement properties, such as movement distance, turn angles, and movement along the arena's perimeter. Instead of examining the effect of treatments on the animal movement, we might in fact examine the animal's interaction with the specific arena used. We advise caution when choosing the right arena and interpreting the results. AbstractMovement is an important animal behavior contributing to reproduction and survival. Animal movement is often examined in arenas or enclosures unde...
Source: Insect Science - May 26, 2023 Category: Biology Authors: Inon Scharf,
Kimberley Hanna,
Daphna Gottlieb Tags: ORIGINAL ARTICLE Source Type: research
Effects of simulated gut pH environment on bacterial composition and pheromone production of Dendroctonus valens
In this study, we fed wild caughtD. valens with 3 different pH media (main host diet with natural pH of 4.7; a mildly acidic diet with pH 6 mimicking the beetle gut pH; and highly acidic diet with pH 4), and measured their effects on the gut pH, bacterial community and production of the main aggregation and anti-aggregation pheromone (verbenone). We further tested the verbenone production capacity of 2 gut bacterial isolates in different pH environments (pH 6 and 4). Compared to natural state or main host diet, feeding on less acidic diet (pH 6) diluted the acidity of the gut, whereas feeding on highly acidic diet (pH 4) e...
Source: Insect Science - May 25, 2023 Category: Biology Authors: Qingjie Cao,
Yu Zhao,
Tuuli ‐Marjaana Koski,
Huiping Li,
Jianghua Sun Tags: ORIGINAL ARTICLE Source Type: research
The selective regulation of immune responses by matrix metalloproteinase MMP14 in Ostrinia furnacalis
In this study, we used the lepidopteran pestOstrinia furnacalis larvae to address these questions by examining the changes of immune-related gene expression and antimicrobial activity after the knockdown ofMMP14 and bacterial infections. We identifiedMMP14 inO. furnacalis using the rapid amplification of complementary DNA ends (RACE), and found that it was conserved and belonged to the MMP1 subfamily. Our functional investigations revealed thatMMP14 is an infection-responsive gene, and its knockdown reduces phenoloxidase (PO) activity andCecropin expression, while the expressions ofLysozyme,Attacin,Gloverin, andMoricin are...
Source: Insect Science - May 21, 2023 Category: Biology Authors: Kangkang Chen,
Shiqi Lu,
Jiahui Song,
Xiaoyi Dou,
Xiangyi Wei,
Xinyan Wang,
Xu Liu,
Congjing Feng Tags: ORIGINAL ARTICLE Source Type: research
Efficient nanoparticle ‐based CRISPR‐Cas13d induced mRNA disruption of an eye pigmentation gene in the white‐backed planthopper, Sogatella furcifera
In this study, the smallest Cas13 family member, Cas13d, and guide RNAs (gRNAs) were complexed with a versatile nanomaterial (star polycation, SPc) to generate a proof-of-concept RNA-editing platform capable of disrupting mRNA expression of the eye pigmentation genetryptophan 2,3-dioxygenase (SfTO) in white-backed planthoppers (WBPHs). The resulting red-eye phenotype was present in 19.76% (with SPc) and 22.99% (without SPc) of the treatment groups and was comparable to the red-eye phenotype generated following conventional RNA interference knockdown (22.22%). Furthermore, the Cas13/gRNA phenotype manifested more quickly th...
Source: Insect Science - May 19, 2023 Category: Biology Authors: Yun ‐Feng Ma,
Meng‐Qi Zhang,
Lang‐Lang Gong,
Xuan‐Zheng Liu,
Gui‐Jun Long,
Huan Guo,
J. Joe Hull,
Youssef Dewer,
Ming He,
Peng He Tags: ORIGINAL ARTICLE Source Type: research
