Immunomodulatory actions of myeloid-derived suppressor cells in the context of innate immunity
Innate Immun. 2023 Nov 28:17534259231215581. doi: 10.1177/17534259231215581. Online ahead of print.ABSTRACTMyeloid-derived suppressor cells (MDSCs) are notable innate immune cells, which are further divided into two subpopulations, i.e., monocytic and granulocytic. These cells are traditionally considered to mainly suppress the T-cell responses. However, more updated data indicate that their properties are rather immunomodulatory than solely immunosuppressive. Indeed, MDSCs display extensive crosstalk with other either innate or adaptive immune cells, and, according to the situation under which they are triggered, they may...
Source: Innate Immunity - November 29, 2023 Category: Allergy & Immunology Authors: Nikoleta Bizymi Andreas M Matthaiou Irene Mavroudi Aristea Batsali Helen A Papadaki Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Nov;29(8):186-200. doi: 10.1177/17534259231207198. Epub 2023 Oct 13.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Nov;29(8):186-200. doi: 10.1177/17534259231207198. Epub 2023 Oct 13.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Nov;29(8):186-200. doi: 10.1177/17534259231207198. Epub 2023 Oct 13.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Nov;29(8):186-200. doi: 10.1177/17534259231207198. Epub 2023 Oct 13.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Nov;29(8):186-200. doi: 10.1177/17534259231207198. Epub 2023 Oct 13.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Nov;29(8):186-200. doi: 10.1177/17534259231207198. Epub 2023 Oct 13.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Nov;29(8):186-200. doi: 10.1177/17534259231207198. Epub 2023 Oct 13.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
