Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Oct 13:17534259231207198. doi: 10.1177/17534259231207198. Online ahead of print.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Oct 13:17534259231205993. doi: 10.1177/17534259231205993. Online ahead of print.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Oct 13:17534259231207198. doi: 10.1177/17534259231207198. Online ahead of print.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Oct 13:17534259231205993. doi: 10.1177/17534259231205993. Online ahead of print.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Oct 13:17534259231207198. doi: 10.1177/17534259231207198. Online ahead of print.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Oct 13:17534259231205993. doi: 10.1177/17534259231205993. Online ahead of print.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic ...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Synthesis and validation of click-modified NOD1/2 agonists
Innate Immun. 2023 Oct 13:17534259231207198. doi: 10.1177/17534259231207198. Online ahead of print.ABSTRACTNOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ravi Bharadwaj Madison V Anonick Swati Jaiswal Siavash Mashayekh Ashley Brown Kimberly A Wodzanowski Kendi Okuda Neal Silverman Catherine L Grimes Source Type: research
Initial exploration of a novel fusion protein, IL-4/IL-34/IL-10, which promotes cardiac allograft survival mice through alloregulation
In conclusion, newly developed FP1 improves the graft survival in cardiac transplantation mouse model. Significant additional work to optimize FP1 or include other novel proteins could supplement current treatment options for transplant patients.PMID:37800911 | DOI:10.1177/17534259231186239 (Source: Innate Immunity)
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Young S Lee I-Ting Cheng Godoy-Ruiz Raquel David J Weber Joseph R Scalea Source Type: research
Obesity Alters cytokine signaling and gut microbiome in septic mice
Innate Immun. 2023 Oct 6:17534259231205959. doi: 10.1177/17534259231205959. Online ahead of print.ABSTRACTSepsis is a leading cause of mortality. Plasma cytokine levels may identify those at increased risk of mortality from sepsis. Our aim was to understand how obesity alters cytokine levels during early sepsis and its correlation with survival. Six-week-old C57BL/6 male mice were randomized to control (non-obese) or high fat diet (obese) for 5-7 weeks. Sepsis was induced by cecal ligation and perforation (CLP). Cytokine levels were measured from cheek bleeds 8 h after CLP, and mice were monitored for survival. Other cohor...
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Lauren Bodilly Lauren Williamson Patrick Lahni Matthew N Alder David B Haslam Jennifer M Kaplan Source Type: research
Initial exploration of a novel fusion protein, IL-4/IL-34/IL-10, which promotes cardiac allograft survival mice through alloregulation
In conclusion, newly developed FP1 improves the graft survival in cardiac transplantation mouse model. Significant additional work to optimize FP1 or include other novel proteins could supplement current treatment options for transplant patients.PMID:37800911 | DOI:10.1177/17534259231186239 (Source: Innate Immunity)
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Young S Lee I-Ting Cheng Godoy-Ruiz Raquel David J Weber Joseph R Scalea Source Type: research
Obesity Alters cytokine signaling and gut microbiome in septic mice
Innate Immun. 2023 Oct 6:17534259231205959. doi: 10.1177/17534259231205959. Online ahead of print.ABSTRACTSepsis is a leading cause of mortality. Plasma cytokine levels may identify those at increased risk of mortality from sepsis. Our aim was to understand how obesity alters cytokine levels during early sepsis and its correlation with survival. Six-week-old C57BL/6 male mice were randomized to control (non-obese) or high fat diet (obese) for 5-7 weeks. Sepsis was induced by cecal ligation and perforation (CLP). Cytokine levels were measured from cheek bleeds 8 h after CLP, and mice were monitored for survival. Other cohor...
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Lauren Bodilly Lauren Williamson Patrick Lahni Matthew N Alder David B Haslam Jennifer M Kaplan Source Type: research
Initial exploration of a novel fusion protein, IL-4/IL-34/IL-10, which promotes cardiac allograft survival mice through alloregulation
In conclusion, newly developed FP1 improves the graft survival in cardiac transplantation mouse model. Significant additional work to optimize FP1 or include other novel proteins could supplement current treatment options for transplant patients.PMID:37800911 | DOI:10.1177/17534259231186239 (Source: Innate Immunity)
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Young S Lee I-Ting Cheng Godoy-Ruiz Raquel David J Weber Joseph R Scalea Source Type: research
Obesity Alters cytokine signaling and gut microbiome in septic mice
Innate Immun. 2023 Oct 6:17534259231205959. doi: 10.1177/17534259231205959. Online ahead of print.ABSTRACTSepsis is a leading cause of mortality. Plasma cytokine levels may identify those at increased risk of mortality from sepsis. Our aim was to understand how obesity alters cytokine levels during early sepsis and its correlation with survival. Six-week-old C57BL/6 male mice were randomized to control (non-obese) or high fat diet (obese) for 5-7 weeks. Sepsis was induced by cecal ligation and perforation (CLP). Cytokine levels were measured from cheek bleeds 8 h after CLP, and mice were monitored for survival. Other cohor...
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Lauren Bodilly Lauren Williamson Patrick Lahni Matthew N Alder David B Haslam Jennifer M Kaplan Source Type: research
Initial exploration of a novel fusion protein, IL-4/IL-34/IL-10, which promotes cardiac allograft survival mice through alloregulation
In conclusion, newly developed FP1 improves the graft survival in cardiac transplantation mouse model. Significant additional work to optimize FP1 or include other novel proteins could supplement current treatment options for transplant patients.PMID:37800911 | DOI:10.1177/17534259231186239 (Source: Innate Immunity)
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Young S Lee I-Ting Cheng Godoy-Ruiz Raquel David J Weber Joseph R Scalea Source Type: research
Obesity Alters cytokine signaling and gut microbiome in septic mice
Innate Immun. 2023 Oct 6:17534259231205959. doi: 10.1177/17534259231205959. Online ahead of print.ABSTRACTSepsis is a leading cause of mortality. Plasma cytokine levels may identify those at increased risk of mortality from sepsis. Our aim was to understand how obesity alters cytokine levels during early sepsis and its correlation with survival. Six-week-old C57BL/6 male mice were randomized to control (non-obese) or high fat diet (obese) for 5-7 weeks. Sepsis was induced by cecal ligation and perforation (CLP). Cytokine levels were measured from cheek bleeds 8 h after CLP, and mice were monitored for survival. Other cohor...
Source: Innate Immunity - October 6, 2023 Category: Allergy & Immunology Authors: Lauren Bodilly Lauren Williamson Patrick Lahni Matthew N Alder David B Haslam Jennifer M Kaplan Source Type: research
