Utility of polymerase chain reaction assays for confirmation of liquid culture test results for Ureaplasma spp.
Swati Khullar, Jyoti Rawre, Neena Khanna, Vishnubhatla Sreenivas, Benu DhawanIndian Journal of Medical Microbiology 2020 38(2):235-236 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Swati Khullar Jyoti Rawre Neena Khanna Vishnubhatla Sreenivas Benu Dhawan Source Type: research

Cryptococcosis in non-human immunodeficiency virus-infected patients: A clinical dilemma and diagnostic enigma
Manisha Paul, Mohit Bhatia, Ranjana Rohilla, Udayakumar Sasirekha, Neelam KaisthaIndian Journal of Medical Microbiology 2020 38(2):229-234 Cryptococcosis is a fungal disease with worldwide distribution and wide array of clinical manifestations, caused by encapsulated basidiomycetous yeasts called Cryptococcus spp. It has traditionally been considered an opportunistic infection known to occur in immunocompromised hosts, particularly those who are infected with human immunodeficiency virus. However, this infection has also been reported in phenotypically 'normal' or otherwise clinically non-immunocompr...
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Manisha Paul Mohit Bhatia Ranjana Rohilla Udayakumar Sasirekha Neelam Kaistha Source Type: research

Catheter-related Brevibacterium casei bloodstream infection in a child with aplastic anaemia
We report a catheter-related bloodstream infection with Brevibacterium casei in a 6-year-old child with aplastic anaemia. Treatment with appropriate antibiotics along with the removal of the catheter resulted in complete cure in our patient. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Sangeeta Joshi Ruchira Misra Suverna Kirolikar Sujata Mushrif Source Type: research

Burkholderia pseudomallei septic arthritis in Type-2 diabetes mellitus patients: Report of two cases
K Baruah Frincy, Borkakoty Biswajyoti, Satyaki Saikia, Manash P Baruah, Utpala DeviIndian Journal of Medical Microbiology 2020 38(2):222-225 Two cases of Burkholderia pseudomallei septic arthritis are presented with a brief review of the literature. B. pseudomallei septic arthritis most commonly occurs in diabetics and other immunocompromised patients and may prove fatal despite appropriate therapy. Clinical and microbiological suspicion of B. pseudomallei infection may help in providing appropriate empirical therapy. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: K Baruah Frincy Borkakoty Biswajyoti Satyaki Saikia Manash P Baruah Utpala Devi Source Type: research

SARS-CoV-2 viral sepsis with meningoencephalitis
Mitkumar Dharsandiya, Kinjal Shah, Ketan Patel, Tushar Patel, Amrish Patel, Atul PatelIndian Journal of Medical Microbiology 2020 38(2):219-221 SARS-CoV-2 predominantly involves the lungs producing acute lung injury, but it can also give rise to a variety of complications involving the central nervous system, gastrointestinal system, kidney and also viral sepsis. With this case report, we are discussing unusual series of complication from acute lung injury, followed by viral sepsis then encephalitis, followed by progressive macrophage activation syndrome. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Mitkumar Dharsandiya Kinjal Shah Ketan Patel Tushar Patel Amrish Patel Atul Patel Source Type: research

Community-acquired infection caused by small-colony variant of Staphylococcus aureus
Sumit Rai, Pramod Kumar Sharma, Nirpex Tyagi, Sugandh AgarwalIndian Journal of Medical Microbiology 2020 38(2):216-218 Staphylococcus aureus and other Gram negative bacteria produce small colony variants (SCV) which usually emerge after exposure to antimicrobials. They cause repeated infections, treatment failures and often pass unnoticed during cultures due to unusual appearance and incomplete incubation. This infectious disease grand round highlights a similar clinical case with atypical history and appearance of a SCV of S. aureus and why prolonged incubation is necessary for aspirates from patients with recurrent infe...
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Sumit Rai Pramod Kumar Sharma Nirpex Tyagi Sugandh Agarwal Source Type: research

Functional studies of Plasmodium falciparum & #39;s prohibitin1 and prohibitin 2 in yeast
Savitha Chellappan, Subarna Roy, Jyoti M Nagmoti, Wahida Tabassum, SL Hoti, Mrinal Kanti Bhattacharyya, Praveen Balabaskaran NinaIndian Journal of Medical Microbiology 2020 38(2):213-215 Prohibitins (PHBs) are evolutionarily conserved mitochondrial integral membrane proteins, shown to regulate mitochondrial structure and function, and can be classified into PHB1 and PHB2. PHB1 and PHB2 have been shown to interact with each other, and form heterodimers in mitochondrial inner membrane. Plasmodium falciparum has orthologues of PHB1 and PHB2 in its genome, and their role is unclear. Here, by homology modelling and yeast two-h...
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Savitha Chellappan Subarna Roy Jyoti M Nagmoti Wahida Tabassum SL Hoti Mrinal Kanti Bhattacharyya Praveen Balabaskaran Nina Source Type: research

Genomic evolution of severe acute respiratory syndrome Coronavirus 2 in India and vaccine impact
Jobin John Jacob, Karthick Vasudevan, Balaji Veeraraghavan, Ramya Iyadurai, Karthik GunasekaranIndian Journal of Medical Microbiology 2020 38(2):210-212 Recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and subsequent containment procedures have impacted the world as never seen before. Therefore, there is considerable curiosity about the genome evolution related to the origin, transmission and vaccine impact of this virus. We have analysed genome sequences of SARS-CoV-2 isolated from Indian patients to gain an in-depth understanding of genomic evolution and transmission in India. Phylogeneti...
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Jobin John Jacob Karthick Vasudevan Balaji Veeraraghavan Ramya Iyadurai Karthik Gunasekaran Source Type: research

Biofilm synthesis and other virulence factors in multidrug-resistant uropathogenic enterococci isolated in Northern India
Conclusion: VanA and vanB are the prevalent genotypes responsible for vancomycin resistance. The high prevalence of MDR enterococcal strains producing biofilm and virulence determinants raises concern. asa1, hyl, esp, gelE, cyl and other genes are known to express these factors and contribute to biofilm formation. Most uropathogenic enterococci expressed biofilm at moderate level and can be detected effectively by calcofluor-white staining. No correlation was noted between vancomycin resistance and biofilm production. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Ayan Kumar Das Mridu Dudeja Sunil Kohli Pratima Ray Manvi Singh Preet Simran Kaur Source Type: research

Prevalence of oxacillin-susceptible methicillin-resistant Staphylococcus aureus nasal carriage and their clonal diversity among patients attending public health-care facilities
Conclusions: There is a gradual colonisation shift happening in the infection pattern by S. aureus in Brazil. The Brazilian Epidemic Clone (ST239-SCCmec IIIa-PVL-) seems to be substituted by isolates from different clonal complexes, such as ST5, ST8 and ST30. The non-correlation between phenotypic/genotypic resistance profile observed in some isolates suggests the presence of other methicillin resistance mechanisms different from mecA presence or a difference in the nucleotide sequence, which prevents the primers to identify the specific region during polymerase chain reaction reactions. MRSA identification should be based...
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Aline Pecanha Muzy Dias Lorrayne Cardoso Guimar & #227;es Livia B. D V. Petrucci J & #233;ssica A Z. Pinheiro Marcos Gabriel Pinheiro Felipe Rodrigues E Silva Helv & #233;cio C C. P & #243;voa F & #225;bio Aguiar-Alves Source Type: research

Molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from patients at a tertiary care hospital in Hyderabad, South India
Conclusions: ST8 was a dominant type along with other previously reported types ST22, ST239, and ST772 from India. The observations highlight the prevalence of genetically diverse clonal populations of MRSA, suggesting potential multiple origins. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Ganapuram J Archana Akhauri Yash Sinha Madhavi Annamanedi Kandala Pavan Asrith Satyajit B Kale Nitin V Kurkure Swapnil P Doijad Kammili Nagamani Nagendra R Hegde Source Type: research

Prevalence, toxin gene profile, genotypes and antibiotic susceptibility of Clostridium difficile in a tertiary care hospital in Taif, Saudi Arabia
Conclusion: Further larger studies are required for an accurate understanding of CDI epidemiology in Saudi Arabia. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Taisir Saber Yousry A Hawash Khadiga A Ismail Amany S Khalifa Khalaf F Alsharif Saleh A Alghamdi Tamer Saber Emad M Eed Source Type: research

Epidemiology and risk factors associated with NTM pulmonary and extrapulmonary infections in a high tuberculosis endemic Region
Conclusions: NTM plays a significant role in causing pulmonary and extrapulmonary infections even in our part of the country with high endemicity of tuberculosis. NTM has emerged as important pathogens even in the immunocompetent patients. There is a need for rapid diagnosis and susceptibility testing of NTM to aid physicians administer timely and appropriate treatment to the patients. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Sanam Rasool Wani Chand Wattal Reena Raveendran Source Type: research

Genotypic and phenotypic characterisation of clinical isolates of methicillin-resistant Staphylococcus aureus in two different geographical locations of Iran
Conclusion: The high prevalence of MRSA makes it necessary to revisit the antibiotics administration by physicians. Indeed, periodic evaluation of antibacterial susceptibility patterns of the MRSA strains is required for efficient treatment of MRSA infections. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Shiva Ahmadishoar Nadia Kazemi Pour Javid Sadeghi Mohammad Reza Nahaei Babak Kheirkhah Source Type: research

Investigation of antimicrobial resistance and virulence genes of Campylobacter isolates from patients in a tertiary hospital in Edirne, Turkey
Conclusions: It was detected that there is an increase in antimicrobial resistance of Campylobacter strains in our region, and most of the isolates harbour virulence genes. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: Canan Eryildiz Kiymet Tabakcioglu Gulcan Kuyucuklu Nermin Sakru Source Type: research