Utility of GeneXpert in the diagnosis, reliance on urine microscopy and clinical characteristics of genitourinary tuberculosis at a tertiary care hospital
Conclusion: The sample size in the present study is small to arrive at a brisk inference, but it may safely be postulated that yield of detection for GeneXpert may be improved using multiple sampling, especially the early morning ones. It is also pertinent to mention here that GeneXpert may not be able to pick up mutant genomes. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Ajaz Nabi Koul Basharat A Kassana Aadil Rafeeq Rather Source Type: research

Echinocandins: Their role in the management of Candida biofilms
Subramanian Swaminathan, Shweta Kamat, Nalini Adele PintoIndian Journal of Medical Microbiology 2018 36(1):87-92The importance of antifungal agents and their clinical implications has received little attention in comparison to antibiotics, particularly in the health-care setting. However, apart from bacterial infections rising in hospitals, the incidences of fungal infections are growing with the development of resistance to conventional antifungal agents. Newer antifungal agents such as echinocandins (ECs) have been extensively studied over the past decade and are recognised as a superior treatment compared with prior ant...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Subramanian Swaminathan Shweta Kamat Nalini Adele Pinto Source Type: research

Identification of multiple strains of Porphyromonas gingivalis using heteroduplex polymerase chain reaction in varying severity of chronic periodontitis
Conclusions: Species-specific PCR and heteroduplex analysis provide a simple and accurate method to analyse the strain diversity of P. gingivalis. P. gingivalis was detected in both healthy periodontal sites as well as sites with periodontitis. The presence of two or three P. gingivalis strains was seen in 60% of the samples. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Mihir Raghavendra Kulkarni Kishore G Bhat Betsy Sara Thomas G Subraya Bhat Raghavendra Dhirendra Kulkarni Source Type: research

Comparison of different methods of RNA preparation from peripheral blood for nucleic acid amplification assay
Conclusions: These results demonstrate that despite the lower purity and integrity of RNA, our RNA preparation protocol is simple and rapid and shows reasonable performance in RT-LAMP. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Dong Eun Lee Hanna Lee Seon Duk Lee Hyung Soo Han Jae Young Choe Sora Yun Hong-Jun Park Jungbae Park Jong Kun Kim Source Type: research

Changing trends of culture-positive typhoid fever and antimicrobial susceptibility in a tertiary care North Indian Hospital over the last decade
Conclusion: We found that the most common etiological agent of enteric fever is S. Typhi causing the majority of cases from July to October in our region. MIC to ceftriaxone in typhoidal salmonellae is creeping towards resistance and more data are needed to understand the azithromycin susceptibility. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Priyanka Sharma Sushila Dahiya Neelam Manral Bhavana Kumari Sambuddha Kumar Sangeeta Pandey Seema Sood Bimal Kumar Das Arti Kapil Source Type: research

Nonspecific amplification of human DNA by Streptococcus pneumoniae LytA primer
Conclusion: Since HRMA is done without a specific probe, one should be aware of non-specific amplifications while using primers for HRMA of human clinical samples. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Helen Hencida Thangamony Ravindran Kumar Chinnappan Palaniappan Thangavelu Mani Mariappa Berlin Grace Viswanathan Mariammal Kootallur Narayanan Brahmadathan Source Type: research

Histoplasmosis in non-endemic North-Western part of India
Conclusion: This study highlights that Gujarat and Rajasthan are an endemic region for histoplasmosis, and a systematic study is required to understand epidemiology of the disease. Histoplasmosis should be a differential diagnosis in a patient presenting with adrenal enlargement, lymphadenopathy, oral ulcers and fever of unknown origin in this region. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Atul K Patel Ketan K Patel Harsh Toshniwal Swati Gohel Arunaloke Chakrabarti Source Type: research

Molecular characterisation for clonality and transmission dynamics of an outbreak of Klebsiella pneumoniae amongst neonates in a tertiary care centre in South India
Conclusion: Extended-spectrum β-lactamase K. pneumoniae is commonly seen in Indian hospitals and main mechanisms being production of SHV, TEM and CTX-M enzymes as seen in the present study. Outer membrane porins contribute significantly to antimicrobial resistance. Emergence of new clones such as ST2647 implies continuous evolution of the organism and also potential for rapid genetic recombination leading to multidrug resistance. Outbreaks amongst neonates lead to fatal outcome, and stringent hospital infection control is necessary. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Chaitra Shankar Manish Kumar Ashtawarthani Baskaran Miracle Magdelene Paul Nithya Ponmudi Sridhar Santhanam Joy Sarojini Michael Balaji Veeraraghavan Source Type: research

Cytomegalovirus reactivation and disease amongst patients with allogeneic haematopoietic stem cell transplantation in Eastern India: Epidemiology, outcome and healthcare cost
Conclusion: In an increasingly globalised world, where medical tourism is common, data from developing countries regarding cost and outcome of CMV infections in AHSCT patients are of relevance. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Meet Kumar Mita Roychowdhury Jeevan Kumar Anusha Harishankar Subir Sinha Saurabh Jayant Bhave Anupam Chakrapani Vivek Radhakrishnan Reena Nair Sanjay Bhattacharya Mammen Chandy Source Type: research

High-level aminoglycoside resistance in Acinetobacter baumannii recovered from Intensive Care Unit patients in Northeastern India
Conclusions: This study reveals the presence of aminoglycoside-resistance genes in combination with blaCTXM and blaNDM, which are highly stable and not frequently reported from this geographical region. Further, the study could predict limited treatment option and need for formulating infection control strategy. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Supriya Upadhyay Annie Bakorlin Khyriem Prithwis Bhattacharya Amitabha Bhattacharjee Santa Ram Joshi Source Type: research

Molecular characterisation and phylogenetic analysis of dengue outbreak in Pasighat, Arunachal Pradesh, Northeast India
Conclusions: The present study reveals a shift in the serotype dominance in the study region. As serotype shifts and secondary infection with a heterologous DENV serotype are frequently associated with disease severity, there is an urgent need for sustained monitoring of the circulating serotypes and enhanced surveillance operations, especially in the monsoon and post-monsoon periods to prevent large-scale, severe dengue outbreaks in this region. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Biswajyoti Borkakoty Mandakini Das Kishore Sarma Aniruddha Jakharia Palash Kumar Das Chandrakanta Bhattacharya Basumoti Apum Dipankar Biswas Source Type: research

Antimicrobial susceptibility profiles of gram-negative bacteria causing infections collected across India during 2014 & #8211;2016: Study for monitoring antimicrobial resistance trend report
Conclusion: Increasing rates of ESBL producers have been noted, which is alarming. Further, carbapenem resistance was also gradually increasing, which needs much attention. Overall, this study data show that carbapenems, amikacin and colistin continue to be the best agents available to treat drug-resistant infections. Thus continuous monitoring of susceptibility profile of the clinically important Gram-negative pathogens is of great importance to guide effective antimicrobial therapy. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Balaji Veeraraghavan Mark Ranjan Jesudason John Antony Jude Prakasah Shalini Anandan Rani Diana Sahni Agila Kumari Pragasam Yamuna Devi Bakthavatchalam Rajesh Joseph Selvakumar TN Dhole Camilla Rodrigues Indranil Roy Sangeetha Joshi Bhaskar Narayan Chaudh Source Type: research

Molecular characterisation of Staphylococcus aureus using spa typing as a diagnostic tool in Haryana, India
Conclusion: High diversity observed within S. aureus isolates indicated that many different strains circulate in the study region or in the hospital. The results would contribute in the understanding of epidemiology related to S. aureus spread and prevention. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Gurjeet Singh Shobha Broor Priti Agarwal Source Type: research

Changing panorama for surveillance of device-associated healthcare infections: Challenges faced in implementation of current guidelines
Renu Gupta, Sangeeta Sharma, Parwez , Sonal SaxenaIndian Journal of Medical Microbiology 2018 36(1):18-25Healthcare-associated infections (HAI) are preventable in up to 30% of patients with evidence-based infection prevention and control (IPC) activities. IPC activities require effective surveillance to generate data for the HAI rates, defining priority areas, identifying processes amenable for improvement and institute interventions to improve patient's safety. However, uniform, accurate and standardised surveillance methodology using objective definitions can only generate meaningful data for effective execution ...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Renu Gupta Sangeeta Sharma Parwez Sonal Saxena Source Type: research

Fusarium: The versatile pathogen
Ananya Tupaki-Sreepurna, Anupma Jyoti KindoIndian Journal of Medical Microbiology 2018 36(1):8-17Fusarium is an emerging human opportunistic pathogen of growing importance, especially among immunosuppressed haematology patients due to an increased incidence of disseminated infections over the past two decades. This trend is expected only to continue due to the advances in medical and surgical technologies that will prolong the lives of the severely ill, making these patients susceptible to rare opportunistic infections. Production of mycotoxins, enzymes such as proteases, angio-invasive property and an intrinsically resist...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Ananya Tupaki-Sreepurna Anupma Jyoti Kindo Source Type: research

Sexually transmitted infections: Need for extragenital screening
Jyoti Rawre, Sonu Agrawal, Benu DhawanIndian Journal of Medical Microbiology 2018 36(1):1-7Extragenital infections can occur concurrently with simultaneous urogenital infections. Extragenital sites are believed to serve as hidden reservoirs and play a critical role in their transmission. The etiological relationship of the most widespread Sexually transmitted diseases (STD) pathogen to reproductive tract has long been established, but the distribution to extragenital sites appears to be infrequent and its correlation with the sexual practice still requires to be investigated. Optimal-screening strategies for extragenital i...
Source: Indian Journal of Medical Microbiology - May 2, 2018 Category: Microbiology Authors: Jyoti Rawre Sonu Agrawal Benu Dhawan Source Type: research

Implementation challenges in bio-medical waste management rules, 2016
Malini R Capoor, Kumar Tapash BhowmikIndian Journal of Medical Microbiology 2017 35(4):623-625 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Malini R Capoor Kumar Tapash Bhowmik Source Type: research

Cladophialophora bantiana brain abscess: A report of two cases treated with voriconazole
Ram Gopalakrishnan, Nandini Sethuraman, R Madhumitha, Kalpesh Sukhwani, Nitin Bansal, Indira Poojary, Arunaloke ChakrabartiIndian Journal of Medical Microbiology 2017 35(4):620-622Cerebral phaeohyphomycosis is an infection caused by a number of dematiaceous fungi, characterised by the presence of melanised hyphae in the invaded tissue. Cladophialophora bantiana is the most common species affecting the humans, which has a predilection for causing the central nervous system infections resulting in high mortality. We hereby report a success story of two cases of brain abscess caused by C. bantiana who were treated with surgic...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Ram Gopalakrishnan Nandini Sethuraman R Madhumitha Kalpesh Sukhwani Nitin Bansal Indira Poojary Arunaloke Chakrabarti Source Type: research

Diagnosis and treatment of diffusible Penicillium marneffei in human immunodeficiency virus-negative patients: A challenge for the physician
Xiao-Hua Chi, Yao-Ming Xue, Quan-Shi Wang, Gui-Ping Li, Hong-Sheng Zhou, Yong-Shuai QiIndian Journal of Medical Microbiology 2017 35(4):617-619Penicillium marneffei infection in human immunodeficiency virus (HIV)-negative patients is addressed far less often. In this article, a small cohort of HIV-negative patients who disseminated P. marneffei infection was included. Sites of infection were found from blood culture, as subcutaneous nodules, or from lymph node biopsy. Fever, rash, swollen lymph nodes, anaemia and weight loss were common characteristics in most infected patients. The signs and symptoms are diverse and creat...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Xiao-Hua Chi Yao-Ming Xue Quan-Shi Wang Gui-Ping Li Hong-Sheng Zhou Yong-Shuai Qi Source Type: research

Increased recognition of Chryseobacterium species as an emerging cause of nosocomial urinary tract infection following introduction of matrix-assisted laser desorption/ionisation-time of flight for bacterial identification
Harsimran Kaur, Balvinder Mohan, Vinaykumar Hallur, Atul Raj, Madhunarayana Basude, Ravimohan S Mavuduru, Neelam TanejaIndian Journal of Medical Microbiology 2017 35(4):610-616Chryseobacterium species are rarely reported as aetiological agents of nosocomial urinary tract infection. Here, we evaluated the clinical significance of 19 isolates of Chryseobacterium species (15 Chryseobacterium indologenes and 4 Chryseobacterium gleum; identified by matrix-assisted laser desorption/ionisation-time of flight [MALDI-TOF]) obtained from urine or percutaneous nephrostomy drainage of 16 patients with urological complaints. The strain...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Harsimran Kaur Balvinder Mohan Vinaykumar Hallur Atul Raj Madhunarayana Basude Ravimohan S Mavuduru Neelam Taneja Source Type: research

Demographic profile of healthy children with nasopharyngeal colonisation of Streptococcus pneumoniae: A research paper
Radhika Raman, Janani Sankar, Sulochana Putlibai, Vaidehi RaghavanIndian Journal of Medical Microbiology 2017 35(4):607-609Background: Pneumonia is a preventable cause of mortality in children. Streptococcus pneumoniae colonising the nasopharynx of healthy children can cause invasive diseases and the serotype distribution of colonisation isolates should be an indicator of invasive disease, antibiotic resistance profiles, and potential vaccine coverage. Identifying factors influencing nasopharyngeal colonisation, the serotypes and antimicrobial resistance pattern can improve rational preventive strategies. Objectives: Ident...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Radhika Raman Janani Sankar Sulochana Putlibai Vaidehi Raghavan Source Type: research

Infections in live donor liver transplant recipients: A study of timeline, aetiology and antimicrobial resistance of bacterial and fungal infections from the developing world
Vikas Khillan, Pratibha Kale, Viniyendra Pamecha, Neha Rathor, Shiv Kumar SarinIndian Journal of Medical Microbiology 2017 35(4):604-606Infections are the leading cause of morbidity and mortality in liver transplant (LT) recipients. We studied timeline, spectrum of infection, system involved, and antimicrobial resistance in 64 patients undergoing live donor LT with 6-month follow-up. Of 64 patients, 38 (59.5%) patients had 103 infectious episodes, 10 patients had single infectious episode and 28 patients had two or more infectious episodes. 96 (93.2%) were bacterial and Candida infections were in 7 (6.8%). Earl...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Vikas Khillan Pratibha Kale Viniyendra Pamecha Neha Rathor Shiv Kumar Sarin Source Type: research

Molecular identification and detection of virulent factors in Helicobacter pylori from gastric biopsy samples of patients attended at Assam Medical College and Hospital, Dibrugarh, Assam, India
Anisha Sarma, Lahari Saikia, Ratna Kanta Bhuyan, Ezaz HussainIndian Journal of Medical Microbiology 2017 35(4):600-603The present study aimed to detect the major virulence determinants of Helicobacter pylori, the gastric bacteria by polymerase chain reaction from genomic DNA of 314 gastric biopsies from dyspeptic patients in 2015-2016 through upper gastrointestinal endoscopy. In 153 cases out of 314, the high prevalence of oipA gene followed by cagA-vacA s1 m1 combined genotypes was found in mostly gastritis/duodenitis patients followed by the peptic ulcer and normal patients. Therefore, the clinical significance of the vi...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Anisha Sarma Lahari Saikia Ratna Kanta Bhuyan Ezaz Hussain Source Type: research

An improvised medium for axenic cultivation of Acanthamoeba spp.
Kirti Megha, Amit Gupta, Rakesh Sehgal, Sumeeta KhuranaIndian Journal of Medical Microbiology 2017 35(4):597-599Acanthamoebae can be easily grown in bacterised cultures, but their growth in axenic media is tedious and many times unsuccessful. We thus experimented with some additives in the conventional axenic medium for growth of various isolates of Acanthamoeba. Addition of Torula yeast RNA was found to significantly enhance the growth of Acanthamoebae in the axenic culture medium. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Kirti Megha Amit Gupta Rakesh Sehgal Sumeeta Khurana Source Type: research

Melioidosis: Reinfection going incognito as relapse
Isra Halim, Tushar Shaw, Chaitanya Tellapragada, KE Vandana, Chiranjay MukhopadhyayIndian Journal of Medical Microbiology 2017 35(4):593-596Melioidosis has recently gained importance as an emerging disease in India. Recurrent melioidosis has been reported from different parts of the world and can be due to relapse or reinfection. Distinction between relapse and reinfection is important for epidemiology, investigation and management. Here, we present the data regarding rate of recurrence and utility of multilocus sequence typing (MLST) in differentiating relapse form reinfection amongst melioidosis patients from a tertiary ...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Isra Halim Tushar Shaw Chaitanya Tellapragada KE Vandana Chiranjay Mukhopadhyay Source Type: research

Clinically relevant yeast species identified by sequencing the internal transcribed spacer region of r-RNA gene and Vitek 2 compact (YST card) commercial identification system: Experience in a Tertiary Care Hospital in Assam, Northeast India
Reema Nath, Reeta Bora, Biswajyoti Borkakoty, Lahari Saikia, Pratap ParidaIndian Journal of Medical Microbiology 2017 35(4):588-592In this retrospective study from 2012 to 2015, 333 clinical isolates of yeasts were identified using Vitek 2 Compact System YST ID card (Biomerieux, France) and internal transcribed spacer (ITS) sequencing. Eighteen species were identified by ITS sequencing. Candida albicans was the most common species (46.5%), followed by Candida tropicalis (27%). The total species supported by Vitek System was 11 (61.11%). The sensitivity of the system in identifying these 11 species was 66.66%...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Reema Nath Reeta Bora Biswajyoti Borkakoty Lahari Saikia Pratap Parida Source Type: research

Dosing strategy based on prevailing aminoglycoside minimum inhibitory concentration in India: Evidence and issues
This study provides insights into the inconsistencies existing in the laboratory interpretation and the corresponding clinical success rates. This urges the need for revising clinical breakpoints for amikacin, to resolve under dosing leading to clinical failure. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Balaji Veeraraghavan Agila Kumari Pragasam Abi Manesh Priscilla Rupali Ramya Iyadurai Camilla Rodrigues Sangeeta Joshi Indranil Roy Bhaskar Narayan Chaudhuri DS Chitnis Dhole Tapan Source Type: research

Effect of Interleukin-28B polymorphism on Interleukin-28 expression and immunological recovery amongst HIV-1-infected individuals following antiretroviral therapy
Conclusion: Our preliminary findings suggest that IL-28 polymorphisms could influence both immunological recovery and therapeutic response in HIV infection. Hence, functional studies are warranted to understand the mechanistic basis of IL-28-mediated host genetic influence on HIV therapeutic response. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: BV Srinidhi G John Fletcher Jaiprasath Sachidanantham Priscilla Rupali Veena Vadhini Ramalingam JP Demosthenes OC Abraham Susanne A Pulimood Grace Rebekah Rajesh Kannangai Source Type: research

Genetic diversity and allelic variation in south Indian isolates of Group A streptococci causing invasive disease
Conclusions: Our study shows that subtle allelic variations in the house keeping genes results in the development of new strains in a given emm type and contribute significantly to the existing high diversity of strains circulating in the community. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Reena Rajkumari John Melbin Jose Kootallur Narayanan Brahmadathan Source Type: research

Evaluation of genotype MTBDRplus line probe assay in detection of rifampicin and isoniazid resistance in comparison to solid culture drug susceptibility testing in a tertiary care centre of western Uttar Pradesh
Conclusion: GenoType® MTBDRplus has good sensitivity and specificity in detecting MDR-TB cases with a significantly lesser turnaround time as compared to conventional DST method and simultaneous detection of Rif and INH resistance. This technique saves several weeks of time required for culture and DST. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Shariq Ahmed Indu Shukla Nazish Fatima Sumit K Varshney Mohammad Shameem Source Type: research

Seroprevalence, risk factors and genotype distribution for Hepatitis C infection: A study from rural hospital in Maharashtra
Conclusions: In the present study, significant risk factors were a history of blood transfusion, habit of alcohol, dialysis. The prevention of HCV infection can be achieved by screening of blood and blood products and creating awareness about risk factors. Since the efficacy of current and new therapies differ by genotype, genotype study is essential. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Satish Ramchandrra Patil Kailash D Datkhile MV Ghorpade Supriya Satish Patil Satish V Kakade Source Type: research

Fecal carriage of carbapenem-resistant Enterobacteriaceae and risk factor analysis in hospitalised patients: A single centre study from India
Conclusions: The study is the first from India to show high CRE carriage in patients admitted to a tertiary care centre and emphasises the need of strict antimicrobial stewardship implementation in hospitals to prevent dissemination of multidrug-resistant CRE. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Balvinder Mohan Amber Prasad Harsimran Kaur Vinaykumar Hallur Neha Gautam Neelam Taneja Source Type: research

Fasciolopsiasis in children: Clinical, sociodemographic profile and outcome
Conclusion: All patients recovered well except one who died due to severe PEM and disseminated tuberculosis and two cases presented with relapse. Most of the cases of polyparasitism were associated with tuberculosis. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Kumar Saurabh Shilpi Ranjan Source Type: research

Profiling of Aggregatibacter actinomycetemcomitans Serotypes B and C and the genotypes in periodontal health and disease
Conclusion: Serotype c was predominant in periodontally diseased as well as periodontally healthy individuals. An association could be present between genotype – serotype and genotype – periodontal status. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Swati Setty Tanvee Wadikar SS Suprith Kishore Bhat Srinath Thakur Source Type: research

Identification of medically important Candida species by polymerase chain reaction-restriction fragment length polymorphism analysis of the rDNA ITS1 and ITS2 regions
Conclusion: When the ability to identify Candida to species level of phenotypic and PCR-RFLP methods was assessed, a great difference was found between these two methods. It may be argued that Msp I and Bln I restriction enzyme fragments can be used in the identification of medically important Candida species. Further studies are needed to develop this kind of restriction profile to be used in the identification of candidal strains. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Suphi Bayraktar Nizami Duran G & #252;lay G & #252;lbol Duran Naciye Eryilmaz Hayat Aslan Cansu & #214;nlen Bur & #231;in & #214;zer Source Type: research

Aetiological study of viruses causing acute encephalitis syndrome in North West India
Conclusions: AES occurred sporadically in Rajasthan, samples should be tested first for herpes group of viruses followed by EV or/and for arboviruses depending on season or measles, mumps and RVs in children. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Jitendra Kumar Tiwari Bharti Malhotra Aradhana Chauhan Hemant Malhotra Pratibha Sharma Farah Deeba Khushbu Trivedi Anjenya M Swamy Source Type: research

The bioinformatics analyses reveal novel antigen epitopes in major outer membrane protein of Chlamydia trachomatis
Conclusion: We established the methods by using the biological information network technologies for looking the T-cell antigen epitopes and B-cell antigen epitopes in MOMP of CT, and three novel T-cell epitopes as well as three novel B-cell epitopes were identified in the current study. It provides important information for further studying the antigenicity of CT MOMP protein and also provides useful information for developing highly efficient subunit vaccines for CT. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Tao ZHang Huijun Li Xi Lan CHuntao ZHang ZHangsheng Yang Wenyan Cao Ning Fen Yumei Liu Yi Yan Amanguli ยท Yasheng Xiumin Ma Source Type: research

Hypertonic xylose agar medium: A novel medium for differentiation of Candida dubliniensis from Candida albicans
Conclusion: Hence, we propose HXAM as a sole phenotypic method for identifying C. dubliniensis from germ-tube-positive isolates or from stock collections of known C. albicans. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Abiroo Jan Gulnaz Bashir Bashir Ahmad Fomda Dekyong Angmo Urgain Khangsar Munazah Manzoor Amrish Kohli Sulmaz Reshi Mohd Suhail Saba Choudhary Akeela Fatima Source Type: research

Distribution of different genes responsible for invasive characteristics, detection of point mutations in capsular gene wchA and biofilm production among the invasive and non-invasive isolates of Streptococcus pneumoniae
Conclusion: The results of our study implies a possible capsular variations among the isolates and this may have an impact on capsular typing. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: James John Kripa Shanker Kasudhan Reba Kanungo Savitri Sharma Vaishali Dohe K Prashanth Source Type: research

Incidence of ventilator-associated pneumonia and impact of multidrug-resistant infections on patient & #39;s outcome: Experience at an Apex Trauma Centre in North India
Conclusions: Isolation of MDR pathogens from bronchoalveolar lavage does not always adversely affect the outcome of patients. It requires an interdisciplinary team of clinical microbiologists, physicians and hospital infection control nurses, to collectively manage these patients. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Surbhi Khurana Purva Mathur Subodh Kumar Kapil Dev Soni Richa Aggrawal Priyam Batra Nidhi Bhardwaj Source Type: research

Pre-transplant cytomegalovirus immunoglobulin G antibody levels could prevent severe cytomegalovirus infections post-transplant in liver transplant recipients: Experience from a tertiary care liver centre
Conclusions: Higher pre-Tx CMV IgG levels might prevent severe CMV infections post-Tx. Recipients with low pre-Tx CMV titre might be benefitted by CMV prophylaxis or aggressive pre-emptive treatment. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Ekta Gupta Viniyendra Pamecha Yogita Verma Niteen Kumar Archana Rastogi Nadeem Hasnian Ajeet Singh Bhadoria Source Type: research

Microbiology, clinical spectrum and outcome of peritonitis in patients undergoing peritoneal dialysis in India: Results from a multicentric, observational study
Conclusion: This large multicentre study of peritonitis offers insights into the aetiology and outcomes of infectious complications of chronic PD in India that are germane to clinical decision-making. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Georgi Abraham Amit Gupta Kashi Nath Prasad Anusha Rohit Anil Kumar Bhalla Vishwanath Billa Rajasekhar Chakravati Tonmoy Das Thadakanathan Dhinakaran Arup Ratan Dutta Padmanabhan Giri Gokulnath Tarun Jeloka Sampath Kumar Ajay Marwaha Radha Vijay Raghavan Source Type: research

Correlation of in vitro sensitivity of chloroquine and other antimalarials with the partner drug resistance to Plasmodium falciparum malaria in selected sites of India
Conclusion: The correlation between IC50and molecular markers for antimalarial drug resistance is reported for the first time through this study. A positive correlation between in vitro drug resistance with molecular markers for antimalarial drug resistance could make in vitro assay a reliable tool to predict drug efficacy which is needed for detection of emerging resistance in the country. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Supriya Sharma Ram Suresh Bharti Nitin Bhardwaj Anupkumar R Anvikar Neena Valecha Neelima Mishra Source Type: research

Spectrum of infections in acute febrile illness in central India
Conclusion: It is important to know the cause and clinical profile of AUFIs for their proper management also it will help to prevent morbidity and mortality in AUFI cases. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Yogendra Pandurang Shelke Vijayshri Suresh Deotale Deepashri Laxmanrao Maraskolhe Source Type: research

Stenotrophomonas maltophilia: From trivial to grievous
Lipika Singhal, Parvinder Kaur, Vikas GautamIndian Journal of Medical Microbiology 2017 35(4):469-479Stenotrophomonas maltophilia, once regarded as an organism of low virulence, has evolved as a significant opportunistic pathogen causing severe human infections in both hospital and community settings, especially amongst highly debilitated patients. Globally, S. maltophilia ranks third amongst the four most common pathogenic non-fermenting Gram-negative bacilli (NFGNBs), others being Pseudomonas aeruginosa, Acinetobacter baumannii and Burkholderia cepacia complex (Bcc). The worth of accurate identification of S. maltophilia...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Lipika Singhal Parvinder Kaur Vikas Gautam Source Type: research

An update on technical, interpretative and clinical relevance of antimicrobial synergy testing methodologies
Shakti Laishram, Agila Kumari Pragasam, Yamuna Devi Bakthavatchalam, Balaji VeeraraghavanIndian Journal of Medical Microbiology 2017 35(4):445-468Testing for antimicrobial interactions has gained popularity in the last decade due to the increasing prevalence of drug-resistant organisms and limited options for the treatment of these infections. In vitro combination testing provides information, on which two or more antimicrobials can be combined for a good clinical outcome. Amongst the various in vitro methods of drug interactions, time-kill assay (TKA), checkerboard (CB) assay and E-test-based methods are most commonly use...
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Shakti Laishram Agila Kumari Pragasam Yamuna Devi Bakthavatchalam Balaji Veeraraghavan Source Type: research

Our microbial signatures
Prabha DesikanIndian Journal of Medical Microbiology 2017 35(4):443-444 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - February 1, 2018 Category: Microbiology Authors: Prabha Desikan Source Type: research

In vitro fosfomycin susceptibility against carbapenem-resistant or extended-spectrum beta & #8211;lactamase-producing gram-negative fosfomycin-naive uropathogens: An alluring option or an illusion
Manisa Sahu, Sanjith Saseedharan, Pallavi BhalekarIndian Journal of Medical Microbiology 2017 35(3):437-438 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - October 12, 2017 Category: Microbiology Authors: Manisa Sahu Sanjith Saseedharan Pallavi Bhalekar Source Type: research

Multidrug-resistant Candida auris: Need for alert among microbiologists
Kamini Walia, Anuradha Chowdhary, VC Ohri, Arunaloke ChakrabartiIndian Journal of Medical Microbiology 2017 35(3):436-436 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - October 12, 2017 Category: Microbiology Authors: Kamini Walia Anuradha Chowdhary VC Ohri Arunaloke Chakrabarti Source Type: research

Multiple parasitic and viral infections in a patient living with HIV/AIDS on antiretroviral therapy
K Deepika, Nonika Rajkumari, AS Liji, Subhash Chandra Parija, Abdoul HamideIndian Journal of Medical Microbiology 2017 35(3):432-435Patients with human immunodeficiency virus (HIV) infection are more prone for gastrointestinal infections causing diarrhoea, particularly with parasites. Parasitic infections have been regularly reported in such patients. A female patient confirmed positive for HIV 1 on antiretroviral therapy came with complaints of chronic diarrhoea for the past 7 months. Her initial CD4 count was 89 cells/μl of blood, and antibodies to cytomegalovirus and herpes simplex virus 1 and 2 virus were found to...
Source: Indian Journal of Medical Microbiology - October 12, 2017 Category: Microbiology Authors: K Deepika Nonika Rajkumari AS Liji Subhash Chandra Parija Abdoul Hamide Source Type: research