Staphylococcus aureus colonisation in HIV-infected patients: Incidence, risk factors and subsequent skin- and soft-tissue infections
Aashirwad Panigrahy, Sanjeev Sinha, Bimal Kumar Das, Arti Kapil, Sreenivas Vishnubhatla, Benu DhawanIndian Journal of Medical Microbiology 2020 38(3):444-447 We evaluated the incidence and risk factors of Staphylococcus aureus colonisation in 300 treatment-naïve HIV patients. Swabs from anterior nares and pharynx were cultured. Eighty-eight patients (29.3%) were colonised with S. aureus (47.7% nasal, 23.8% pharyngeal and 28.5% at both sites), which yielded 112 isolates. Methicillin-resistant S. aureus was detected in 25.9% (29/112) of isolates. Panton�...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Aashirwad Panigrahy Sanjeev Sinha Bimal Kumar Das Arti Kapil Sreenivas Vishnubhatla Benu Dhawan Source Type: research

Characterisation of colonisation properties of vaginal lactobacilli from healthy Indian women: Implications for identification of potential probiotic candidates
Arati Mane, Ishrat Khan, Madhuri ThakarIndian Journal of Medical Microbiology 2020 38(3):440-443 The ability of probiotic bacteria to colonise the target site is considered of utmost importance and used for preliminary screening of potential probiotic candidates. Thirty-eight vaginal Lactobacillus strains isolated from healthy women and characterised by 16S RNA sequencing were assessed for colonisation characteristics including adherence to human vaginal epithelial cells, auto-aggregation and hydrophobicity. No significant difference in adherence (P = 0.384), auto-aggregation (P = 0.282) and hydropho...
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Arati Mane Ishrat Khan Madhuri Thakar Source Type: research

Two novel genomic DNA sequences as common diagnostic targets to detect Cryptosporidium hominis and Cryptosporidium parvum: Development of quantitative polymerase chain reaction assays, and clinical evaluation
Conclusion: Novel target-based qPCR assays can be rapid an efficient tool for simultaneous detection of a C. parvum and C. hominis. These genes could also be utilized for the development of innovative DNA-based Point-of-Care test development. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Arpit Kumar Shrivastava Swagatika Panda Subrat Kumar Priyadarshi Soumyaranjan Sahu Source Type: research

Molecular, phylogenetic and antibiotic resistance analysis of enteroaggregative escherichia coli/uropathogenic Escherichia coli hybrid genotypes causing urinary tract infections
Conclusions: Our results indicated a closer relationship among EAEC and UPEC, which suggested that some EAEC strains can be potential uropathogens. Ours is a first study documenting the existence of EAEC pathotypes VGs in UPEC strains of nosocomial origin; further studies are required to understand the diarrhoeagenic potential of these hybrids. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Vinay Modgil Harpreet Kaur Balvinder Mohan Neelam Taneja Source Type: research

Characteristics and outcomes of critically ill patients with influenza A (H1N1) in the Western Balkans during the 2019 post-pandemic season
Conclusion: Critically ill patients with confirmed influenza A (H1N1) pdm09 infection in the post-pandemic season were older, required vasoactive drugs more often, and there was a trend of higher survival compared to H1N1 infection patients in the previous pandemic seasons. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Pedja Kovacevic Jovan Matijasevic Sasa Dragic Biljana Zlojutro Srdjan Gavrilovic Milka Jandric Ana Andrijevic Tijana Kovacevic Vladimir Carapic Maja Travar Ljubisa Preradovic Danica Momcicevic Source Type: research

Microsporidia infection in patients with autoimmune diseases
Conclusion: There was a significant increase in microsporidia infection in autoimmune disease patients undergoing treatment with TNF-α antagonists, and the duration of treatment is one of the risk factors. The study highlights the importance of microsporidia testing in immunocompromised patients, particularly those undergoing treatment with anti-TNF-α drugs and emphasises the need for awareness among clinicians regarding this opportunistic parasite. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Khadiga Ahmed Ismail Yousry A Hawash Taisir Saber Emad M Eed Amany S Khalifa Khalaf F Alsharif Saleh A Alghamdi Ahmed M Khalifa Osama Mahmoud Khalifa Hatem K Althubiti Gala M Alsofyani Source Type: research

Molecular diversity of dengue virus serotypes 1 & #8211;4 during an outbreak of acute dengue virus infection in Theni, India
Conclusion: Our study documents the molecular characteristics of DENV circulating in our geographical locality. The detection of heterologous DENV serotypes highlights the importance of regular molecular monitoring for the early recognition of any switch in pre-dominant serotype. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Amudhan Murugesan Dhanasezhian Aridoss Swarna Senthilkumar Lallitha Sivathanu Ramalingam Sekar Esaki M Shankar Elanchezhiyan Manickan Source Type: research

Use of fluorescence foldscope as an effective tool for detection of biofilm formation in Pseudomonas aeruginosa
Conclusion: Fluorescence foldscopy is an effective and reliable tool for the detection of biofilm formation in clinical isolates of P. aeruginosa under different laboratory conditions. Biofilm-forming P. aeruginosa worsens the medical condition and is difficult to eradicate. The present study came up with an effective and reliable tool for the detection of biofilm formation in clinical isolates of P. aeruginosa. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Chandrayee Deshamukhya Bhaskar Jyoti Das Shiela Chetri Deepjyoti Paul Debadatta Dhar Chanda Tuhina Banerjee Amitabha Bhattacharjee Source Type: research

Detection of various beta-Lactamases in Escherichia coli and Klebsiella sp.: A study from Tertiary Care Centre of North India
Conclusion: Our finding shows high percentages of ESBL and carbapenemases in E. coli and Klebsiella spp. Among phenotypic methods, CDT seems to be a better choice as prevalence of carbapenemases shows lots of variation in our country. For Class B enzymes, both CDT and mCIM/eCIM can be used in the routine laboratories. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Varsha Gupta Meenakshi Singh Priya Datta Anku Goel Sanjay Singh Kashinath Prasad Jagdish Chander Source Type: research

Comparison of two real-time polymerase chain reaction assays for the detection of severe acute respiratory syndrome-CoV-2 from combined nasopharyngeal-throat swabs
Conclusions: The results highlight the need for careful evaluation of commercial kits before being deployed for screening of COVID-19 infections. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Oves Siddiqui Vikas Manchanda Abhishek Yadav Tanu Sagar Sanchita Tuteja Nazia Nagi Sonal Saxena Source Type: research

Prevalence of torch infections and its associated poor outcome in high-risk pregnant women of Central India: Time to think for prevention strategies
Conclusion: A high prevalence of IgM seropositivity of TORCH infection was noted in the present study with the increased rate of poor foetal outcome warrants the need of proper prenatal counselling, universal immunisation and nutritional supplements during pregnancy. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: HV Manjunathachar Kavita N Singh Vivek Chouksey Ravindra Kumar Ravendra K Sharma Pradip V Barde Source Type: research

Antimicrobial activity of biosynthesised silver nanoparticles against multidrug-resistant microbes isolated from cancer patients with bacteraemia and candidaemia
Conclusion: The present study revealed a significant distribution of multidrug-resistant microbes, which may increase the burden of healthcare to prevent infections in cancer patients. Biosilver nanoparticles exhibit antimicrobial activity against multidrug-resistant microbes and could be considered as effective agents against these strains. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Gamal Mohamed El-Sherbiny Mohamed Kasem Lila Yousseria Mohamed Shetaia Marwa M F. Elswify Samar Samer Mohamed Source Type: research

The impact of antimicrobial stewardship programme on regulating the policy adherence and antimicrobial usage in selected intensive care units in a tertiary care center - A prospective interventional study
Conclusions: Implementation of continuous monitoring of the AMS programme, therefore, has a definite role in reducing the antimicrobial consumption and improving the compliance to the policy guidelines. A more robust study for a prolonged period is, however, necessary to have a better analysis of the outcome. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Sushmita Sana Chowdhury Apurba Sankar Sastry Sathasivam Sureshkumar Anusha Cherian Sujatha Sistla Deepashree Rajashekar Source Type: research

Group B Streptococcus in Indian pregnant women: Its prevalence and risk factors
Conclusion: Due to the low GBS prevalence and no significant association with major risk factors, we recommend to institute universal screening of GBS in pregnant women, instead of risk based screening. Since this was a single centric study with low prevalence of GBS, its applicability may be limited, therefore further larger multi-centric prospective studies are required to understand the true GBS prevalence in Indian society. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Neeraj Goel Chand Wattal Kanwal Gujral Nehal Dhaduk Chandra Mansukhani Pankaj Garg Source Type: research

Epidemiological pattern of Malassezia, its phenotypic identification and antifungal susceptibility profile to azoles by broth microdilution method
Conclusion: AFST is important as it will help the dermatologist to choose the appropriate antifungal agents for the patient and thereby reduce the chronicity of the disease with good patient compliance (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - November 4, 2020 Category: Microbiology Authors: Packia Nancy Romald Anupma Jyoti Kindo V Mahalakshmi U Umadevi Source Type: research