Molecular investigation of staphylococcal cassette chromosome mec types and genotypic relations of methicillin-resistant staphylococci isolated from before and after hospital exposed students
Conclusion: Most of the isolates obtained after hospital training showed clonal similarity and seven had multiple SCCmec elements require further investigation for the possible mechanism. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Bora Ekinci Tuba Gokdogan Edgunlu Gulcin Bayramoglu Gulsen Ulucam Atay Source Type: research

Assessment of microbiological status after successful completion of intermittent revised national tuberculosis control programme directly observed treatment, short course regimen for microbiologically confirmed pulmonary tuberculosis cases: While new daily regimen going to be implemented in India
Conclusion: Till date, RNTCP does not follow up the patients for any period of time after successful completion of treatment. Through the present study, we could find relapse cases in 10% of the previously treated non-symptomatic patients. These unnoticed relapse cases have potential to spread TB and increase disease burden of country. Thus, we can conclude that RNTCP has to follow up the patients after successful treatment to determine whether they relapse. It is needed for the success of programme and control of the disease in the country. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Roumi Ghosh Sudipta Roy Md. Khalid Rashid Source Type: research

Intestinal cryptosporidiosis in renal transplant recipients: Prevalence, species detection and comparative evaluation of SSU rRNA and Cryptosporidium oocyst wall protein genes
Conclusions: Cryptosporidiosis is common amongst RT recipients, particularly those with diarrhoea. C. hominis is the most common species in the studied population. SSU rRNA PCR was more sensitive molecular method for the differentiation of Cryptosporidium species. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Ujjala Ghoshal Prabhat Ranjan Asmita Dey Uday Chand Ghoshal Source Type: research

Performance of an in-house real-time PCR assay for detecting Cytomegalovirus infection among transplant patients from a tertiary care centre
Conclusion: The in-house viral load assay had a better correlation with clinical findings compared to the commercial assay. Quality assessment of these assays was done by the United Kingdom National External Quality Assessment Scheme (UKNEQAS), an external proficiency testing programme, and by the National Institute for Biological Standard and Control (NIBSC) standard. For UKNEQAS and NIBSC standards, the bias between the assays was 0.73 log10and 0.85 log10, respectively. This difference is well within the acceptable range already reported in the literature. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Santhosh Kumar Duraisamy Shoba Mammen Sasi Kumar Reddy Lakshminarayan Susan Verghese Mahesh Moorthy Biju George Rajesh Kannangai Santosh Varghese Alok Srivastava Asha Mary Abraham Source Type: research

Co-circulation of four dengue serotypes at South Eastern Andhra Pradesh, India: A prospective study
Conclusion: This study reports the all four dengue serotypes' co-circulation. This is the first report from South Eastern Andhra Pradesh. Amongst four, DENV-2 was predominant followed by DENV-4. The information of predominant serotypes can guide in forecasting dengue outbreaks and improving control measures of vectors thus may be helpful in the prevention of outbreaks. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Rishi Gowtham Racherla Madhavi Latha Pamireddy Alladi Mohan Nagaraja Mudhigeti Padmalatha Anjaneyulu Mahalakshmi Umapathi Nallapireddy Usha Kalawat Source Type: research

Detection of anti-hepatitis C virus and hepatitis C virus RNA in dried blood spot specimens using Whatman No. 1 filter paper
Conclusions: DBS using Whatman No. 1 filter paper is quite reliable as serum for detection of anti-HCV. It can be useful in effective surveillance. However, it is not suitable for confirmation of chronic HCV infection. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Ritapa Ghosh Naba Kumar Hazarika Source Type: research

The effectiveness of sofosbuvir and daclatasvir in the treatment of hepatitis C in thalassaemia major patients and their effect on haematological factors
Conclusion: Combination of SOF and DAC is an effective and tolerable treatment regimen without affect on the amount of anaemia in patients and did not differentiate the need for blood transfusions. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Farhad Zamani Hossein Ajdarkosh Fahimeh Safarnezhad-Tameshkel Azita Azarkeivan Hossein Keyvani Farshad Naserifar Jamshid Vafaeimanesh Source Type: research

Lamivudine plus tenofovir versus lamivudine plus adefovir for the treatment of hepatitis B virus in HIV-coinfected patients, starting antiretroviral therapy
Conclusions: Adefovir plus lamivudine is an effective alternative of tenofovir plus lamivudine in long-term HBV treatment outcome in HIV/HBV coinfected patients. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Jayeeta Sarkar Debraj Saha Bhaswati Bandyopadhyay Bibhuti Saha Runu Chakravarty Subhasish Kamal Guha Source Type: research

Rapid detection of ethambutol-resistant Mycobacterium tuberculosis in clinical specimens by real-time polymerase chain reaction hybridisation probe method
Conclusions: Our real-time PCR hybridisation probe method promises for rapid detection of EMB-resistant MTB directly from clinical specimens. However, future studies and modifications of method by incorporating other potential loci along with targeted mutation (emb306) are still required to increase the sensitivity of method. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: DS Chauhan R Sharma D Parashar R Das P Sharma AV Singh PK Singh K Katoch VM Katoch Source Type: research

Polymorphisms in cytotoxic T-lymphocyte associated antigen 4 gene does not affect scytotoxic T-lymphocyte associated antigen 4 levels in human papillomavirus-infected women with or without cervical cancer
Conclusion: The present study suggests sCTLA-4 levels are not affected by a polymorphism at + 49 A>G CTLA-4. Hence, levels of CTLA-4 are similar in both CaCx cases and control group. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Priyanka Wagh Priyanka Kulkarni Shilpa Kerkar Himangi Warke Hemangi Chaudhari Kedar Deodhar Bharat Rekhi Hemant Tongaonkar Jayanti Mania-Pramanik Source Type: research

Prothrombotic state in HIV: A study on protein C, protein S, homocysteine and correlation with CD4 counts
Swati Khare, Rashmi Kushwaha, Ashutosh Kumar, Vimala Venkatesh, Himanshu D Reddy, Mili Jain, Mohd Yusuf, Uma Shankar SinghIndian Journal of Medical Microbiology 2018 36(2):201-206Introduction: Human immunodeficiency virus (HIV) may result in variable haematological manifestations. Thrombotic events are more common among HIV-infected persons than the general population, possibly due to the increased inflammatory/hypercoagulable state and presence of concurrent comorbidities. Aims and Objectives: (1) Screen for coagulation abnormalities in HIV-infected patients. (2) Detect certain prothrombotic factors such as deficiency of ...
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Swati Khare Rashmi Kushwaha Ashutosh Kumar Vimala Venkatesh Himanshu D Reddy Mili Jain Mohd Yusuf Uma Shankar Singh Source Type: research

Identification of Raoultella spp.: Comparison of three methods
Conclusion: It was established that MALDI-TOF MS method is reliable in identifying genus Raoultella. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Alicja Sekowska Agnieszka Mikucka Eugenia Gospodarek-Komkowska Source Type: research

Distribution of hepatitis C virus genotypes in Istanbul, Turkey
Conclusion: The majority of patients with HCV infection had genotype 1 (82.5%), followed by genotype 3, 2 and 4. Monitoring the change in HCV genotype distribution is critical for the development of effective strategies for HCV elimination. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Nuran Karabulut Sema Alacam Ayfer Yolcu Mustafa Onel Ali Agacfidan Source Type: research

Trends in antimicrobial resistance patterns of Group A streptococci, molecular basis and implications
Conclusion: The erythromycin and tetracycline resistance in GAS continues to exist at high levels and may be attributed to the over-prescription and use of these antibiotics. Our findings indicate that the use of these antibiotics especially macrolides as empiric therapy in penicillin-allergic patients may not be appropriate. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Tintu Abraham Sujatha Sistla Source Type: research

Molecular characterisation of virulent gene vacA in Helicobacter pylori clinical isolates from patients with gastroduodenal diseases in Assam, India
Conclusion: Prevalence of VacA s1m1 strains may account for high risk of transmission of this gastric pathogen and the overall risk of acquiring infection. Phylogenetic analysis results suggests the prevalence of high genetic diversity in our region. Our findings may aid in developing a better understanding of the genetic structure of H. pylori and the pathophysiology of associated diseases, thus facilitating the implementation of various treatment options. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 7, 2018 Category: Microbiology Authors: Anisha Sarma Lahari Saikia Mituban Gogoi Kaushal Yadav Source Type: research