Multiple virulence factors regulated by quorum sensing may help in establishment and colonisation of urinary tract by Pseudomonas aeruginosa during experimental urinary tract infection
Conclusion: This study suggests that protease, elastase and rhamnolipid contribute to pathogenesis and survival of P. aeruginosa during urinary tract infection. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - March 15, 2013 Category: Microbiology Authors: P GuptaRK GuptaK Harjai Source Type: research

Multiplex polymerase chain reaction using insertion sequence 6110 (IS6110) and mycobacterial protein fraction from BCG of Rm 0.64 in electrophoresis target genes for diagnosis of tuberculous lymphadenitis
Conclusion: In conclusion, techniques like MPCR with high sensitivity and specificity can play an important role in rapid diagnosis of TBLA. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - March 15, 2013 Category: Microbiology Authors: K SharmaN GuptaA SharmaG SinghPK GuptaA RajwanshiSC VarmaM Sharma Source Type: research

Prevalence of biofilm-producing Staphylococcus epidermidis in the healthy skin of individuals in Tamil Nadu, India
Conclusion: We inferred that ica operon and biofilm-forming S. epidermidis are common in the healthy skin of individuals in Tamil Nadu. Measures have to be taken to reduce the risk of hospital-acquired S. epidermidis infections. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - March 15, 2013 Category: Microbiology Authors: CA EL FarranA SekarA BalakrishnanS ShanmugamP ArumugamJ Gopalswamy Source Type: research

Alteration in sample preparation to increase the yield of multiplex Polymerase Chain Reaction assay for diagnosis of genital ulcer disease
Conclusion: The results indicate that sample preparation using phenol chloroform extraction and ethanol precipitation, prior to M-PCR helps to eliminate the inhibitors and increase the yield of the assay. However, being a laborious procedure, it may be used for samples giving negative results after the screening by Roche Amplicor specimen preparation kit. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - March 15, 2013 Category: Microbiology Authors: G RaoA DasP PrabhakarV NemaAR Risbud Source Type: research

Comparison of two recombinant systems for expression of cholera toxin B subunit from Vibrio cholerae
Conclusion: Expression of rCTB in pAE_ctxB construct was more efficient (15-fold) than pQE_ctxB, and it seems that Lac UV5 in E. coli BL21 (DE3) is more compatible with the former construct. This expression system can be used to produce recombinant CTB in high yield which may enable us to study the oral tolerance or mucosal adjuvant properties of rCTB using animal models. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - March 15, 2013 Category: Microbiology Authors: M BoustanshenasB BakhshiM GhorbaniD Norouzian Source Type: research

Evaluation and characterisation of A and B fragments of Corynebacterium diphtheriae toxin towards recombinant diphtheria vaccine
Conclusion: Our results showed the possibility to prepare a potent recombinant vaccine containing whole DT gene or DT-A against C. diphtheriae or could be used in treatment of cancer as it give high levels of IL-2 and IFN-γ. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - March 15, 2013 Category: Microbiology Authors: S AbulmagdM EmaraS AzizR El-Domany Source Type: research

Challenges to pneumococcal vaccine in India
R KanungoIndian Journal of Medical Microbiology 2013 31(1):1-2 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - March 15, 2013 Category: Microbiology Authors: R Kanungo Source Type: research