Investigation of plasmid-mediated quinolone resistance in Pseudomonas aeruginosa clinical isolates
Conclusions: Plasmid-mediated quinolone resistance genes have so far not been identified in P. aeruginosa isolates. However, qnrB have detected in P. florescens and P. putida isolates. This is the first study conducted on the qnrA, qnrB, qnrS and qnrC genes as well as the qepA and aac (6')-Ib-cr genes in P. aeruginosa clinical isolates. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: Y Tanriverdi CayciAY CobanM Gunaydin Source Type: research

Comparison of manual mycobacteria growth indicator tube and epsilometer test with agar proportion method for susceptibility testing of Mycobacterium tuberculosis
Conclusion: The results have shown that MGIT and Etest methods can be used instead of the agar proportion method, because these two methods are more rapid and easier than the agar proportion method. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: N KarabulutB BayraktarY Bulut Source Type: research

Serological diagnosis of dengue in laboratory practice in Kolkata
Conclusions: Serodiagnostic tests dengue-specific NS1 antigen and IgM and IgG antibodies when conducted simultaneously would be able to diagnose confirmed dengue cases categorising primary and secondary dengue along with the duration of the disease, whether early or prolonged. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: N BhattacharyaH MukherjeeR NaskarS TalukdarG DasN PramanikAK Hati Source Type: research

Association of MDR-TB isolates with clinical characteristics of patients from Northern region of India
Conclusion: Routine surveillance of resistance to anti-TB drugs will improve timely recognition of MDR-TB cases and help prevent further transmission in Northern India. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: A GuptaMR NagarajaP KumariG SinghR RamanSK SinghS Anupurb Source Type: research

Significance of Epstein-Barr virus (HHV-4) and CMV (HHV-5) infection among subtype-C human immunodeficiency virus-infected individuals
J Sachithanandham, R Kannangai, SA Pulimood, A Desai, AM Abraham, OC Abraham, V Ravi, P Samuel, G SridharanIndian Journal of Medical Microbiology 2014 32(3):261-269Purpose: Opportunistic viral infections are one of the major causes of morbidity and mortality in HIV infection and their molecular detection in the whole blood could be a useful diagnostic tool. Objective: The frequency of opportunistic DNA virus infections among HIV-1-infected individuals using multiplex real-time PCR assays was studied. Materials and Methods: The subjects were in two groups; group 1: Having CD4 counts 350 cells/µl (n = 173). Individuals ...
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: J SachithanandhamR KannangaiSA PulimoodA DesaiAM AbrahamOC AbrahamV RaviP SamuelG Sridharan Source Type: research

Transfer patterns of integron-associated and antibiotic resistance genes in S. flexneri during different time intervals in Tianjin, China
Conclusion: Of the 32 strains of S. flexneri isolated from 1981 to 1983 and stored in our laboratory, class 1 integron was detected in 28 strains (87.50%), while 27 strains (84.37%) harboured an aminoglycoside resistance gene, aadA, in the variable region of their integrons. Class 1 integron was identified in 22 (91.67%) of the 24 S. flexneri strains isolated from 2009 to 2010, whereas the variable region and 3′-end amplification were not present in any of the strains. Class 2 integron was not found in the 1981-1983 group (group A) of strains; although 19 (79.17%) of the 24 strains in the 2009-2010 group (group B) po...
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: J WangF LiangX-Mei WuW Qi Source Type: research

Detection of Hepatitis C virus RNA in peripheral blood mononuclear cells of patients with abnormal alanine transaminase in Ahvaz
Conclusion: In the present study high rate of 32% occult hepatitis C were found among the patients with elevated ALT. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: M MakvandiD KhalafkhanyM RastiN NeisiA OmidvariniaAT MirghaedA MasjedizadehAA Shyesteh Source Type: research

Recent outbreak of scrub typhus in North Western part of India
Conclusion: Scrub typhus has emerged as an important cause of febrile illness in Jaipur. Empirical treatment with doxycycline is justified in endemic areas. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: Parul SinhaSweta GuptaRomika DawraPuneet Rijhawan Source Type: research

Antibiotic susceptibility pattern and genotyping of campylobacter species isolated from children suffering from gastroenteritis
Conclusion: PCR assay method allows reliable detection of C. jejuni. C. jejuni was the most prevalent Campylobacter species. Gentamicin, amikacin and chloramphenicol were the most effective antibiotic. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: RM Abd El-BakyM SakhyGFM Gad Source Type: research

Application of PCR fingerprinting using (GACA) 4 primer in the rapid discrimination of dermatophytes
Conclusion: The PCR-based genotype using the short primer is rapid and precise in direct identification of dermatophyte isolates by one-step PCR to the species level and strain discrimination of the T. mentagrophytes variants. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: E ElavarashiAJ KindoJ KalyaniR Sudha Source Type: research

Meta-analysis in microbiology
N Pabalan, H Jarjanazi, TS SteinerIndian Journal of Medical Microbiology 2014 32(3):229-235The use of meta-analysis in microbiology may facilitate decision-making that impacts public health policy. Directed at clinicians and researchers in microbiology, this review outlines the steps in performing this statistical technique, addresses its biases and describes its value in this discipline. The survey to estimate extent of the use of meta-analyses in microbiology shows the remarkable growth in the use of this research methodology, from a minimal Asian output to a level comparable with those of Europe and North America in the...
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: N PabalanH JarjanaziTS Steiner Source Type: research

"Chennai Declaration": 5-year plan to tackle the challenge of anti-microbial resistance
Chennai Declaration TeamIndian Journal of Medical Microbiology 2014 32(3):221-228 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: Chennai Declaration Team Source Type: research

The New ICMJE Recommendations
Jacob Rosenberg, Howard Bauchner, Joyce Backus, Peter De Leeuw, Jeff Drazen, Frank Frizelle, Fiona Godlee, Charlotte Haug, Astrid James, Christine Laine, Humberto Reyes, Peush Sahni, Getu ZhaoriIndian Journal of Medical Microbiology 2014 32(3):219-220 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - July 10, 2014 Category: Microbiology Authors: Jacob RosenbergHoward BauchnerJoyce BackusPeter De LeeuwJeff DrazenFrank FrizelleFiona GodleeCharlotte HaugAstrid JamesChristine LaineHumberto ReyesPeush SahniGetu Zhaori Source Type: research

Snippets
Prabha DesikanIndian Journal of Medical Microbiology 2014 32(2):213-215 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: Prabha Desikan Source Type: research

Cherish my microbe-philic heart
Someshwaran RajamaniIndian Journal of Medical Microbiology 2014 32(2):212-212 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: Someshwaran Rajamani Source Type: research

Characterisation of heteroresistant subcolonies for MBL, AmpC genes in Klebsiella pneumoniae and Acinetobacter baumannii
J Shanthi, R BalagurunathanIndian Journal of Medical Microbiology 2014 32(2):210-211 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: J ShanthiR Balagurunathan Source Type: research

Evaluation of dried blood spot as an alternative specimen for the diagnosis of anti-HCV in resource-limited settings
P Nandagopal, H Syed Iqbal, S Saravanan, SS Solomon, Shruti Mehta, M Selvakumar, E Chandrasekhar, S Solomon, P BalakrishnanIndian Journal of Medical Microbiology 2014 32(2):208-210 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: P NandagopalH Syed IqbalS SaravananSS SolomonShruti MehtaM SelvakumarE ChandrasekharS SolomonP Balakrishnan Source Type: research

Is low virulence Acinetobacter baumannii acquiring virulence?
GK Badave, VA Agarwal, NG DeogadeIndian Journal of Medical Microbiology 2014 32(2):207-208 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: GK BadaveVA AgarwalNG Deogade Source Type: research

Molecular epidemiology of extended spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in a tertiary care hospital
N Kammili, N Cherukuri, S Palvai, GP Pazhni, T Ramamurthy, JV Rao, PR AnuradhaIndian Journal of Medical Microbiology 2014 32(2):205-207 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: N KammiliN CherukuriS PalvaiGP PazhniT RamamurthyJV RaoPR Anuradha Source Type: research

Prevalence of atypical bacterial pathogens in hospitalised adult patients with community-acquired pneumonia in Central Greece
C Neocleous, I Gerogianni, K Gourgoulianis, E PetinakiIndian Journal of Medical Microbiology 2014 32(2):204-205 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: C NeocleousI GerogianniK GourgoulianisE Petinaki Source Type: research

Comparative in vitro activity of polymyxins against carbapenem susceptible and resistant non-fermenters from critically ill-patients
V Gupta, N Bansal, S Palta, S Gombar, J ChanderIndian Journal of Medical Microbiology 2014 32(2):203-204 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: V GuptaN BansalS PaltaS GombarJ Chander Source Type: research

The prevalence of bacterial contamination of stethoscope diaphragms: A cross sectional study, among health care workers of a tertiary care hospital
PP Lokkur, S NagarajIndian Journal of Medical Microbiology 2014 32(2):201-202 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: PP LokkurS Nagaraj Source Type: research

Nasal carriage of multidrug-resistant MSSA and MRSA in children of municipality schools
M Bharathi, N Lakshmi, C Siva Kalyani, I Jyothi PadmajaIndian Journal of Medical Microbiology 2014 32(2):200-201 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: M BharathiN LakshmiC Siva KalyaniI Jyothi Padmaja Source Type: research

One step away from conquering polio eradication in India
SS Negi, NM NagarkarIndian Journal of Medical Microbiology 2014 32(2):199-199 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: SS NegiNM Nagarkar Source Type: research

Laboratory accreditation programme for human immunodeficiency virus testing in India
A Ganju, AK KangaIndian Journal of Medical Microbiology 2014 32(2):197-198 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: A GanjuAK Kanga Source Type: research

Prompt diagnosis and extraordinary survival from Naegleria fowleri meningitis: A rare case report
We report a case of N. fowleri meningitis in a 6-year-old boy who presented with signs and symptoms of acute bacterial meningitis. No history of travelling or swimming was present. However, the boy frequently played with water stored from a "kuhl" (diversion channels of water). Wet mount of cerebrospinal fluid (CSF) revealed amoeboid and actively motile flagellate forms of trophozoites. CSF culture done on 1.5% non-nutrient agar plates with a lawn culture of Escherichia coli kept at 37°C for 15 days did not reveal any growth. The test of flagellation on passing CSF in distilled water was however positive in ...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: A SoodS ChauhanL ChandelSC Jaryal Source Type: research

Emergence of VIM-2 metallo-beta-lactamase producing Ralstonia pickettii clinical isolate in India
A Khajuria, AK Praharaj, N Grover, M KumarIndian Journal of Medical Microbiology 2014 32(2):191-193A multidrug-resistant clinical isolate of Ralstonia pickettii from a woman was analysed. Modified Hodge test was positive for carbapenemase production. Conjugation experiment revealed the presence of conjugative plasmid of>140 Kb size typed as IncN type. This is the first report of emergence blaVIM-2 in R. pickettii in India. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: A KhajuriaAK PraharajN GroverM Kumar Source Type: research

Cerebral phaeohyphomycosis by Exophiala dermatitidis
We report a case of cerebral phaeohyphomycosis caused by Exophiala dermatitidis in a young immuno competent male presenting to a tertiary care hospital in Jaipur. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: S SoodVK VaidM SharmaH Bhartiya Source Type: research

Lingual cysticercosis
AP Pichare, AV Rujuta, M Sanjeevani, VP BaradkarIndian Journal of Medical Microbiology 2014 32(2):185-187Cysticercosis is a disease caused by larval form of tapeworm. Cysticercus cellulose primarily develops in tissues of pigs. Infection of human tissues is unusual and affliction of the oral cavity is rare. We, herein, present a case of lingual cysticercosis without involvement of any other site. A 5-year-old male, non-vegetarian child presented with a painless, pearly white, solitary nodular swelling on posterior dorsal side of tongue since 3 months. Excision biopsy was done. Histopathology revealed cysticercous cellulose...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: AP PichareAV RujutaM SanjeevaniVP Baradkar Source Type: research

Spirocheatal shock syndrome
AA Alfaifi, I Masoodi, O Alzaidi, S Hussain, S Khurshid, IA SirwalIndian Journal of Medical Microbiology 2014 32(2):183-185In this paper we describe a clinical scenario of high grade fever, jaundice, hypotension and multi-organ dysfunction syndrome in a 30-year-old homeless male without radiological biliary obstruction. He was brought to our hospital by the emergency medical service of Saudi Arabia (Red Crescent) from the street. After an initial resuscitation he proved to have Borrelia recurrentis. The clinical course during his hospital stay was not a smooth one, but the patient finally improved. After 20 days of hospita...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: AA AlfaifiI MasoodiO AlzaidiS HussainS KhurshidIA Sirwal Source Type: research

Fatal missed case of hemophagocytic lymphohistiocytosis co-infected with parvovirus B19 and Epstein-Barr virus in an infant: Test hyperferritinaemia early
J Kishore, D KishoreIndian Journal of Medical Microbiology 2014 32(2):181-183Hemophagocytic lymphohistiocytosis (HLH) triggered by Parvovirus B19 and Epstein-Barr virus co-infection is rare and unknown in infants. A 2-month-old male infant with fever, rash, bicytopenia and hepato-splenomegaly died owing to diagnostic dilemmas. Hence simply testing for hyperferritinaemia and hypertriglyceridemia/hypofibrinogenemia could diagnose HLH early while robust treatment be life-saving. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: J KishoreD Kishore Source Type: research

Brain abscess due to Streptococcus oralis in an immunocompetent patient
R Solanki, S Subramanian, V Lakshmi, V Bhushanam, A KumarIndian Journal of Medical Microbiology 2014 32(2):179-180A bacteriologically proven case of brain abscess, due to Streptococcus oralis is being reported in a 12-year-old girl who is a known case of congenital heart disease. The patient presented with fever, headache and vomiting. Pus cultures yielded S. oralis. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: R SolankiS SubramanianV LakshmiV BhushanamA Kumar Source Type: research

Erratum
Indian Journal of Medical Microbiology 2014 32(2):178-178 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Source Type: research

Prevalence of adhesive genes among uropathogenic Escherichia coli strains isolated from patients with urinary tract infection in Mangalore
AV Shetty, SH Kumar, M Shekar, AK Shetty, I Karunasagar, I KarunasagarIndian Journal of Medical Microbiology 2014 32(2):175-178The study was carried out to detect the adhesive genes pap (pyelonephritis associated pili), sfa (S fimbrial adhesin) and afa (afimbrial adhesin) from Escherichia coli strains isolated in patients diagnosed with urinary tract infection (UTI). A total of 23% of the isolates were positive for pap, sfa and afa genes with a prevalence of 60.87% (14/23), 39.1% (9/23) and 39.1% (9/23), respectively. Prevalence of multiple adhesive genes was 8.7% (2/23) for pap and afa, 30.43% (7/23) for pap and sfa. Sign...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: AV ShettySH KumarM ShekarAK ShettyI KarunasagarI Karunasagar Source Type: research

Incidence of extended spectrum beta lactamase producing Escherichia coli among patients, healthy individuals and in the environment
EA George, S Sankar, MV Jesudasan, C Sudandiradoss, B NandagopalIndian Journal of Medical Microbiology 2014 32(2):172-174We investigated the faecal carriage of extended spectrum β-lactamases (ESBL) producing Escherichia coli in different groups of human subjects and in the environment. A total of 363 E. coli strains were isolated from stool samples of patients (n = 77), healthy subjects (n = 170) and from different environmental samples (n = 116). A total of 124 ESBL producing E. coli strains were isolated in this study. The frequency of ESBL producing E. coli was found to be highest (60.3%) among the strains isolated...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: EA GeorgeS SankarMV JesudasanC SudandiradossB Nandagopal Source Type: research

Assessment of bacteriological quality of ready to eat food vended in streets of Silchar city, Assam, India
I Sharma, JA MazumdarIndian Journal of Medical Microbiology 2014 32(2):169-171A total of 37 street vended food samples were examined for bacterial and the colony forming units counts ranged from 4.5 × 10 5 to 1.12 × 10 6 . The isolates were identified as Escherichia coli (37.5%), Pseudomonas aeruginosa (3.57%), Staphylococcus aureus (14.20%), Salmonella sp. (5.36%), Klebsiella sp. (10.71%), Shigella sp. (19.64%) and Enterobacter sp. (8.93%) respectively. All the 56 isolates were susceptible to ciprofloxacin while their susceptibility to the other drugs varied. These findings demonstrated that the ready-to-eat foo...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: I SharmaJA Mazumdar Source Type: research

Evaluating the role of low-speed centrifugation towards transfecting human peripheral blood mononuclear cell culture
We present a new transfection protocol, wherein, low-speed centrifugation of cell culture plates immediately after adding the lipid: DNA complex significantly enhances the transfection efficiency. Peripheral blood mononuclear cells (PBMCs) were transfected with BLOCK-iT™ Fluorescent Oligo (scrambled siRNA) and lipofectamine complex using conventional and low-speed centrifugation modified transfection protocols. The efficiency of transfection was determined using flowcytometer and cell viability was checked using MTT assay. Incorporation of low-speed centrifugation significantly enhances the transfection efficiency of...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: M MajumdarR RathoY ChawlaMP Singh Source Type: research

Revised ciprofloxacin breakpoints for Salmonella typhi: Its implications in India
B Veeraraghavan, A Sharma, P Ranjan, A KapilIndian Journal of Medical Microbiology 2014 32(2):161-163The rise of multidrug resistant strains of Salmonella Typhi in the last decade of the previous century led to the use of fluoroquinolones as the drug of choice. However, over the past few years fluoroquinolone resistance has been increasingly reported. In accordance with the revised Clinical and Laboratory Standards Institute (CLSI) breakpoints, only 3% of the isolates were susceptible to ciprofloxacin in comparison to 95% as per the earlier guidelines when 488 isolates collected between 2010 and 2012 were re-interpreted. I...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: B VeeraraghavanA SharmaP RanjanA Kapil Source Type: research

Clinicomicrobiological analysis of patients with cholangitis
SM Shenoy, S Shenoy, S Gopal, BV Tantry, S Baliga, A JainIndian Journal of Medical Microbiology 2014 32(2):157-160Acute cholangitis is inflammation of biliary ductal system from infection with an associated biliary obstruction. This retrospective study was done to determine the factors responsible for cholangitis and the microbiological profile of the bile in patients with cholangitis. In the study involving 348 patients, 36.4% had associated malignancy. A total of 54% of the bile samples were positive for aerobic culture. Nearly 66-73% of the Escherichia coli and Klebsiella isolates were Extended spectrum beta lactamases ...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: SM ShenoyS ShenoyS GopalBV TantryS BaligaA Jain Source Type: research

Same day sputum smear microscopy approach with modified ZN staining for the diagnosis of pulmonary tuberculosis in a microscopy centre at Rajahmundry
Conclusions: Diagnosis of lung tuberculosis is possible with two spot sputum samples with modified ZN staining. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: TJ ChandraRS RajYV Sharma Source Type: research

Trends in antimicrobial susceptibility of Salmonella Typhi from North India (2001-2012)
Conclusion: There has been re-emergence of susceptibility to first line antibiotics and a notable decline in MDR strains of S. Typhi. We have a very high resistance to NA and decreasing susceptibility to ciprofloxacin. Third generation cephalosporins and azithromycin seem to be effective therapeutic options. Judicious use of these antibiotics is mandatory to prevent emergence of resistant strains. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: L SinghalPK GuptaP KaleV GautamP Ray Source Type: research

PCR analysis of 16S-23S ribosomal RNA internal transcribed spacer for identification of Acinetobacter clinical isolates
V WiwanitkitIndian Journal of Medical Microbiology 2014 32(2):148-148 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: V Wiwanitkit Source Type: research

Identification of Acinetobacter clinical isolates by polymerase chain reaction analysis of 16S-23S ribosomal ribonucleic acid internal transcribed spacer
Conclusion: These findings confirmed 16S-23S rRNA ITS for the identification of A. baumannii of different genotypes among patients. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: MAA SarhanAA OsmanWO HaimourMN MohamedTH TahaNM Abdalla Source Type: research

Study of complement activation, C3 and interleukin-6 levels in burn patients and their role as prognostic markers
S Modi, M Rashid, A Malik, M ShahidIndian Journal of Medical Microbiology 2014 32(2):137-142Purpose: The management of burn patients is always challenging for the clinician due to high risk of bacterial sepsis, multi-organ failure and death. Our objective was to study complement activation, C3 and interleukin-6 (IL-6) levels in burn patients and evaluate their role as prognostic markers. Materials and Methods: A total of 63 burn patients and 60 healthy controls were included in this study. Blood was collected from patients within 24 h and at 7 th day of injury. Complement activation was determined by crossed electrophoresi...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: S ModiM RashidA MalikM Shahid Source Type: research

A novel nested reverse-transcriptase polymerase chain reaction method for rapid hepatitis C virus detection and genotyping
Conclusion: Though being a two-step process, this method is fast, cost-efficient, reliable and feasible for regular HCV RNA screening and apt even in resource limited settings. This method could be translated to regular nucleic acid screening for other infectious diseases as regular PCR regimen. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: K SahaR FirdausA BiswasA MukherjeePC Sadhukhan Source Type: research

Detection of Enterovirus 71 gene from clinical specimens by reverse-transcription loop-mediated isothermal amplification
Conclusions : The effectiveness of RT-LAMP is higher than RT-PCR and real-time PCR. The protocol is easy to operate and time saving. It was not an expensive instrument, which was needed; it is an applicable method for rapid diagnosis of the disease, especially in resource-poor countries or in developing countries. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: D WangX WangY GengC An Source Type: research

Revamping the role of biofilm regulating operons in device-associated Staphylococci and Pseudomonas aeruginosa
Pradeep P Halebeedu, GS Vijay Kumar, Shubha GopalIndian Journal of Medical Microbiology 2014 32(2):112-123Extensive use of indwelling devices in modern medicine has revoked higher incidence of device associated infections and most of these devices provide an ideal surface for microbial attachment to form strong biofilms. These obnoxious biofilms are responsible for persistent infections, longer hospitalization and high mortality rate. Gene regulations in bacteria play a significant role in survival, colonization and pathogenesis. Operons being a part of gene regulatory network favour cell colonization and biofilm formation...
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: Pradeep P HalebeeduGS Vijay KumarShubha Gopal Source Type: research

South African HIV strains in India: Did clade C follow the mandrax route?
Shobha SehgalIndian Journal of Medical Microbiology 2014 32(2):110-111 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: Shobha Sehgal Source Type: research

Endorsing Chennai declaration and implementing the strategy
Reba KanungoIndian Journal of Medical Microbiology 2014 32(2):109-109 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 2, 2014 Category: Microbiology Authors: Reba Kanungo Source Type: research

Commentary : Slow or long-term non-progressor HIV patients: Indian scenario
S SinghIndian Journal of Medical Microbiology 2014 32(1):77-78 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 4, 2014 Category: Microbiology Authors: S Singh Source Type: research