Paediatric leptospirosis: A population based case-control study from Chennai, India
R Narayanan, G Sumathi, SG Prabhakaran, S Shanmughapriya, K NatarajaseenivasanIndian Journal of Medical Microbiology 2016 34(2):228-232The surveillance in Chennai identified 134 children and 443 adults clinically suspected for leptospirosis. Of these, 35 (26.1%) children and 118 (26.6%) adults had laboratory confirmed diagnosis for leptospirosis. The paediatric leptospirosis exhibited a higher frequency of classic features of Weil's disease. The prevalent serovar encountered was Icterohaemorrhagiae with no difference in the pattern of infecting serovars between the two groups. Further, confirmation of diagnosis was ...
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: R NarayananG SumathiSG PrabhakaranS ShanmughapriyaK Natarajaseenivasan Source Type: research

Antimicrobial resistance trends in blood culture positive Salmonella Paratyphi A isolates from Pondicherry, India
In this study, a total of 284 blood culture isolates of S. Paratyphi A were investigated. Of these isolates, 281 (98.9%) were nalidixic acid resistant. A high rate (6.3%) of high-level resistance (≥4 μg/mL) was found to ciprofloxacin. The isolates with ciprofloxacin minimum inhibitory concentrations (MICs) of ≥12 μg/mL had 4 mutations, 2 mutations within the quinolone resistance-determining region of gyrA and 2 mutations also in parC. According to the Clinical Laboratory Standards Institute 2012 MIC breakpoints, 75.0% of isolates were resistant to ciprofloxacin. Finally, 3 major pulsed-field gel elect...
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: GA MenezesBN HarishMA KhanW GoessensJP Hays Source Type: research

Ribosomal RNA-based panbacterial polymerase chain reaction for rapid diagnosis of septicaemia in Intensive Care Unit patients
Mahua Das Gupta, Harsimran Kaur, Pallab Ray, Vikas Gautam, GD PuriIndian Journal of Medical Microbiology 2016 34(2):219-221Early diagnosis and treatment of sepsis by appropriate antibiotics is of utmost importance. Therefore, we evaluated 16S rRNA panbacterial polymerase chain reaction (PCR) for rapid diagnosis of sepsis in 49 adult patients in Intensive Care Units (ICUs) and compared it with an automated blood culture. 8 ml of 10 ml blood collected was inoculated into BACTEC® aerobic bottle and the remaining 2 ml was used for DNA extraction and PCR. 109 of 115 (93%) episodes of suspected sepsis showed concordant resu...
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: Mahua Das GuptaHarsimran KaurPallab RayVikas GautamGD Puri Source Type: research

An Xpert screen to identify carbapenemases
Mubin Kazi, Chaitali Nikam, Anjali Shetty, Camilla RodriguesIndian Journal of Medical Microbiology 2016 34(2):216-218To prevent the spread of carbapenemases-producing Enterobacteriaceae (CPE) active surveillance, contact isolation and cohorting infected patients should be practiced. Rectal swabs for the Xpert MDRO-assay of 32 patients were included. 71.85% were positive for targets incorporated into the MDRO-assay; whereas 28% were phenotypically not CRE and Xpert negative (9.37% had different mechanism [bla OXA]). The assay identified 59.3%, 9.37% and 3.1% as bla NDM, bla NDM+VIM and bla VIM, respectively. The assay is a ...
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: Mubin KaziChaitali NikamAnjali ShettyCamilla Rodrigues Source Type: research

Chikungunya virus infection in West Bengal, India
Conclusion: Re-emergence and resurgence of the Chikungunya virus requires continuous monitoring. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: S ChattopadhyayR MukherjeeA NandiN Bhattacharya Source Type: research

Typing of Plasmodium falciparum DNA from 2 years old Giemsa-stained dried blood spots using nested polymerase chain reaction assay
D Kumar, S Dhiman, B Rabha, D Goswami, K Yadav, M Deka, V Veer, I BaruahIndian Journal of Medical Microbiology 2016 34(2):210-212A panel of 129 Giemsa-stained thick blood spots (TBS) confirmed for Plasmodium falciparum infection having different levels of parasite density were collected from a malaria endemic area. DNA was extracted and nested polymerase chain reaction (PCR) assay was performed to amplify P. falciparum DNA. Nested PCR assay successfully amplified P. falciparum DNA at a very low parasitaemia of ~10 parasites/μl of blood. Current PCR assay is very simple and can be used retrospectively to monitor the in...
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: D KumarS DhimanB RabhaD GoswamiK YadavM DekaV VeerI Baruah Source Type: research

Fixed-dose combinations of antimicrobials: A need for special attention
Conclusions: There are several inadequately addressed aspects of the considered FDCs which are available in Indian market. In view of the growing problem of antimicrobial resistance, this issue must get the required attention. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: N ShafiqG KumarV GautamP RayS Malhotra Source Type: research

Candida albicans outbreak associated with total parenteral nutrition in the neonatal unit
Conclusion: According to results of the molecular methods, we thought that a C. albicans outbreak had occurred in the neonatal pediatric unit, due to contamination of TPN solution. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: H GuducuogluB GultepeB OtluA BektasO YildirimO TuncerM Berktas Source Type: research

A prospective study of wound infection among post-discharge patients at a level 1 trauma centre of India
Conclusions: Wound infections are common after discharge among trauma patients highlighting the importance of active surveillance and participation of patients. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: K SharmaBT ThanbuanaAK GuptaN RajkumariP MathurJ GunjiyalMC Misra Source Type: research

Mutational prevalence of chloroquine resistance transporter gene among Plasmodium falciparum field isolates in Assam and Arunachal Pradesh, India
Conclusion: Our study has confirmed high prevalence of mutant Pfcrt genotypes associated with chloroquine resistance in Assam and Arunachal Pradesh, India. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: J SharmaM SoniP DuttaSA KhanJ Mahanta Source Type: research

Different characteristics between mycobacteriophage Chy1 and D29, which were classified as cluster A2 mycobacteriophages
Conclusions: The sequence of Chy1 showed 97.4% homology with the genome sequence of D29, but there was a large difference in their biological characteristics. Overall, the results of this investigation indicate that Chy1 is not an ideal candidate for developing mycobacteriophage-based anti-TB therapies but for future researches to investigate the reason why biological characteristics of Chy1 and D29 were remarkably different. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: Y GanP LiuT WuS Guo Source Type: research

Human immunodeficiency virus, hepatitis B virus and hepatitis C virus seroprevalence in critically ill emergency department patients at a Trauma Care Centre, India
Conclusion: Adopting Standard Precautions (SP) can be used as an easy method to decrease the risk of occupationally acquired infections. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: P BatraP MathurS BhoiBT ThanbuanaS NairM BalamurganMC Misra Source Type: research

New Delhi metallo-β-lactamase and extended spectrum β-lactamases co-producing isolates are high in community-acquired urinary infections in Assam as detected by a novel multiplex polymerase chain reaction assay
Conclusion: NDM and ESBL co-producing isolates were both community (64.7%) and hospital (35.29%) acquired among E. coli. Antibiotic resistance can be effectively evaluated by a cost and time effective molecular method, such as the multiplex PCR used in this study, which complement culture and sensitivity tests. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: VV BorahKK SaikiaP ChandraNK HazarikaR Chakravarty Source Type: research

Treatment with lamivudine and entecavir in severe acute hepatitis B
Conclusion: Anti-viral therapy can be considered for managing selected cases of SAHB. Biochemical as well as virological parameters need to orient the choice of the anti-viral agent. Lamivudine displayed a greater decrease in viral load compared to controls, but it was associated with lower levels of HBsAg to anti-HBs seroconversion. Patients treated with entecavir showed a better response in terms of HBs seroconversion by 24 weeks. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: A Streinu-CercelO SandulescuM StefanA Streinu-Cercel Source Type: research

Genotyping and serotyping of macrolide and multidrug resistant Streptococcus pneumoniae isolated from carrier children
Conclusion: A significant proportion of children harboured macrolide and multidrug-resistant S. pneumoniae. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: SF SwedanWA HayajnehGN Bshara Source Type: research

Microbial profile of the vitreous aspirates in culture proven exogenous endophthalmitis: A 10-year retrospective study
Conclusion: A bacterial predominance was observed among causative organisms of exogenous endophthalmitis with Pseudomonas species being the most common. The appropriate surgical intervention improved or stabilised the visual acuity in nearly 50% eyes. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: H BhattacharjeeK BhattacharjeeK GogoiM SinghBG SinglaA Yadav Source Type: research

A recombinant plasmid of composite cysteine proteinase inhibitor/glyceraldehyde-3-phosphate dehydrogenase gene of periodic Brugia malayi functions on DNA immunity in the host
Z Fang, Q Xu, JQ Wu, SJ Lu, YY Wang, H FangIndian Journal of Medical Microbiology 2016 34(2):146-152Objectives: Both cysteine proteinase inhibitors (CPIs) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) play important roles in the pathogenesis of parasites and their relationship with the hosts. We constructed a new eukaryotic recombinant expression plasmid pcDNA3.1(+)-BmCPI/BmGAPDH of periodic Brugia malayi for investigation of the DNA vaccine-elicited immune responses. Materials and Methods: We cloned a gene encoding the CPIs and GAPDH from periodic B. malayi into vector pcDNA3.1. The composited plasmid or the contro...
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: Z FangQ XuJQ WuSJ LuYY WangH Fang Source Type: research

Gonorrhoea diagnostics: An update
R Verma, S SoodIndian Journal of Medical Microbiology 2016 34(2):139-145Diagnosis of gonorrhoea is an ongoing challenge. The organism is fastidious requiring meticulous collection and transport for successful cultivation. Asymptomatic infections are common which go undetected by conventional methods thereby leading to continued transmission and the risk of complications. The nucleic acid amplification tests, now increasingly used in developed countries, offer improved sensitivity compared to bacterial culture. However, these continue to suffer sequence related problems leading to false positive and false negative results. ...
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: R VermaS Sood Source Type: research

Blood-borne viruses and health care workers: A neglected entity!
Ekta Gupta, Amit GuptaIndian Journal of Medical Microbiology 2016 34(2):137-138 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: Ekta GuptaAmit Gupta Source Type: research

Combating the spread of Middle East respiratory syndrome coronavirus: Indian perspective
Baijayantimala MishraIndian Journal of Medical Microbiology 2016 34(2):135-136 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: Baijayantimala Mishra Source Type: research

The revised guidelines of the Medical Council of India for academic promotions: Need for a rethink
Rakesh Aggarwal, Nithya Gogtay, Rajeev Kumar, Peush Sahni, for the Indian Association of Medical Journal Editors Indian Journal of Medical Microbiology 2016 34(2):131-134 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - April 14, 2016 Category: Microbiology Authors: Rakesh AggarwalNithya GogtayRajeev KumarPeush Sahnifor the Indian Association of Medical Journal Editors Source Type: research

Evaluation of the antibiotic resistance of Pseudomonas aeruginosa isolates according to the changing guidelines
K Yanik, Y Tanriverdi Cayci, A Karadag, S Esen, M GunaydinIndian Journal of Medical Microbiology 2016 34(1):123-124 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: K YanikY Tanriverdi CayciA KaradagS EsenM Gunaydin Source Type: research

Presence of a novel variant NDM-10, of the New Delhi metallo-beta-lactamase in a Klebsiella pneumoniae isolate
Atul Khajuria, Ashok Kumar Praharaj, Mahadevan Kumar, Naveen GroverIndian Journal of Medical Microbiology 2016 34(1):121-123 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: Atul KhajuriaAshok Kumar PraharajMahadevan KumarNaveen Grover Source Type: research

NDM-1 Infection and colonisation in critically ill patients from Delhi: A glimpse of the community scenario
P Batra, M Dwivedi, BL Sherwal, R Dutta, S GuptaIndian Journal of Medical Microbiology 2016 34(1):120-121 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: P BatraM DwivediBL SherwalR DuttaS Gupta Source Type: research

Implications of p24 antigen in HIV testing
AA Mathews, AK Mambatta, B Appalaraju, S MenonIndian Journal of Medical Microbiology 2016 34(1):119-119 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: AA MathewsAK MambattaB AppalarajuS Menon Source Type: research

Blood stream infection by Chryseobacterium species in an immunocompetent individual
P Sharma, S Gupta, S Verma, DV Singh, A KangaIndian Journal of Medical Microbiology 2016 34(1):118-118 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: P SharmaS GuptaS VermaDV SinghA Kanga Source Type: research

Pulmonary nocardiosis mimicking malignancy
J Taneja, B Sen, N DangIndian Journal of Medical Microbiology 2016 34(1):117-118 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: J TanejaB SenN Dang Source Type: research

Current trend of antibiotic sensitivity of Salmonella typhi and other Salmonellae in Mumbai: A 5 years study
CA Chande, KA Chopdekar, V Pradnya, R Unnati, B Jyoti, S Ritesh, C Shazia, SG Joshi, C AbhayIndian Journal of Medical Microbiology 2016 34(1):115-116 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: CA ChandeKA ChopdekarV PradnyaR UnnatiB JyotiS RiteshC ShaziaSG JoshiC Abhay Source Type: research

Recent outbreak of scrub typhus in North Western part of India
JH ParkIndian Journal of Medical Microbiology 2016 34(1):114-114 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: JH Park Source Type: research

High level mupirocin resistance among CoNS from nasal carriers of End stage renal disease patients and hospital personnel from tertiary care centre, Chennai
S Murugesan, U Singh, N Perumal, V Ramanathan, P KrishnanIndian Journal of Medical Microbiology 2016 34(1):114-115 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: S MurugesanU SinghN PerumalV RamanathanP Krishnan Source Type: research

Effect of genitourinary flora on occurrence of recurrent urinary tract infection in females
V Gupta, R Garg, A Huria, P Goel, J Chander, SD CruzIndian Journal of Medical Microbiology 2016 34(1):112-113 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: V GuptaR GargA HuriaP GoelJ ChanderSD Cruz Source Type: research

Congenital syphilis in the era of decreasing seroprevalence
A Baidya, A Ghosh, S Chopra, A Garg, S Sood, A Kapil, BK DasIndian Journal of Medical Microbiology 2016 34(1):111-112 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: A BaidyaA GhoshS ChopraA GargS SoodA KapilBK Das Source Type: research

Investigation on the effect of the ecological parameters on the prevalence of Laribacter hongkongensis in freshwater fish and in human
Q Kong, J Sun, L Shen, J Cha, H Xu, H Jin, H Yu, X NiIndian Journal of Medical Microbiology 2016 34(1):110-111 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: Q KongJ SunL ShenJ ChaH XuH JinH YuX Ni Source Type: research

Plasmid profile and antibiogram of Enterococcal faecalis isolated from tertiary care hospital in Delhi
M Barua, S Das, C Gupta, R Saha, IR KaurIndian Journal of Medical Microbiology 2016 34(1):109-110 (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: M BaruaS DasC GuptaR SahaIR Kaur Source Type: research

First report of Dientamoeba fragilis infection explaining acute non-specific abdominal pain
E Vassalou, CM Vassalos, G Spanakos, A Fotopoulos, G Dounias, P Kalofolias, G Vrioni, A TsakrisIndian Journal of Medical Microbiology 2016 34(1):106-108Dientamoeba fragilis is now considered a potentially emerging gastrointestinal pathogen in both developing and developed countries. We first report an autochthonous case of D. fragilis infection in Greece. A 49-year-old female with acute non-specific abdominal pain required emergency surgical admission for active observation and repeated assessment. To the best of our knowledge, this is the first reported case of acute unexplained abdominal pain finally attributed to D. fra...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: E VassalouCM VassalosG SpanakosA FotopoulosG DouniasP KalofoliasG VrioniA Tsakris Source Type: research

Chronic invasive fungal rhinosinusitis by Paecilomyces variotii: A rare case report
This article reviews and presents a case report of chronic fungal sinusitis in an immunocompetent adult male infected with Paecilomyces variotii which is opportunistic soil saprophyte, uncommon to humans. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: T SwamiS PannuMukesh KumarG Gupta Source Type: research

Prosthetic joint infection due to Lysobacter thermophilus diagnosed by 16S rRNA gene sequencing
We report the first case of prosthetic joint infection caused by Lysobacter thermophilus which was identified by 16S rRNA gene sequencing. Removal of prosthesis followed by antibiotic treatment resulted in good clinical outcome. This case illustrates the use of molecular diagnostics to detect uncommon organisms in suspected prosthetic infections. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: B DhawanS SebastianR MalhotraA KapilD Gautam Source Type: research

Papilloma of lip associated with human papilloma viruses-32 infection in a child
Sasidharanpillai Sabeena, Sadashiva Rao Pallade, Nutan Kamath, Mary Mathew, Govindakarnavar ArunkumarIndian Journal of Medical Microbiology 2016 34(1):97-99Squamous papilloma is the most common benign oral epithelial lesion, and it is well known to be associated with human papilloma virus 6 and 11. Here, we report a case of squamous papilloma associated with human papilloma viruses (HPV)-32 in a 4-year-old boy who presented with a verrucous lesion on the lower lip. HPV-32 is often associated with a rare benign condition focal epithelial hyperplasia (FEH). A limited number of lesions and the absence of characteristic histol...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: Sasidharanpillai SabeenaSadashiva Rao PalladeNutan KamathMary MathewGovindakarnavar Arunkumar Source Type: research

Leprous neuromyositis: A rare clinical entity and review of the literature
We reported a case of 56-year-old gentleman presenting with pyrexia of unknown origin, asymmetric sensory motor axonal polyneuropathy and was on chronic exogenous steroid therapy. On evaluation, his skin, muscle, nerve and bone marrow biopsy showed numerous globi of acid-fast Bacilli suggestive of leprous neuromyositis, a rare form of disseminated Hansen's disease. We reported this case in view of its rarity, atypical manifestation of a relatively rare disease and literature review on poor electrophysiological correlation in the diagnosis of leprous neuromyositis as compared to the histopathological examination. (Sourc...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: Shubhanker MitraKarthik GunasekaranGeeta ChackoSamuel George Hansdak Source Type: research

Detection of rifampicin resistance in tuberculosis by molecular methods: A report from Eastern Uttar Pradesh, India
R Tripathi, P Sinha, R Kumari, P Chaubey, A Pandey, S AnupurbaIndian Journal of Medical Microbiology 2016 34(1):92-94Diagnosis of drug resistance tuberculosis (TB) by the gold standard method is labour intensive and time consuming. Hence, there is an urgent need for introduction of rapid diagnostic techniques. Line probe assay (LPA) and cartridge-based nucleic acid amplification test (CBNAAT) have been introduced in India under Revised National Tuberculosis Control Program. Spot and morning sputum samples of previously treated patients by anti-TB drugs were subjected to LPA or CBNAAT. Total 682/1253 (54.4%) were diagno...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: R TripathiP SinhaR KumariP ChaubeyA PandeyS Anupurba Source Type: research

Co-infection of scrub typhus and leptospirosis in patients with pyrexia of unknown origin in Longding district of Arunachal Pradesh in 2013
Conclusion: The present study is the first report of co-infection of scrub typhus with leptospirosis from Northeast India. Medical officers in this region should take scrub typhus and leptospirosis in their differential diagnosis of patients with PUO for early diagnosis and effective treatment. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: Biswajyoti BorkakotyAniruddha JakhariaDipankar BiswasJagadish Mahanta Source Type: research

Resistance to amoxicillin-clavulanate and its relation to virulence-related factors in Yersinia enterocolitica biovar 1A
N Singhal, M Kumar, JS VirdiIndian Journal of Medical Microbiology 2016 34(1):85-87Recent studies have reported that the virulence factors (VFs) were detected more frequently in amoxicillin-clavulanate (AMC) susceptible clinical isolates of Escherichia coli. Here, we have evaluated the relationship between VFs and AMC-resistance phenotype in clinical isolates of Y. enterocolitica biovar 1A. The presence/absence of VFs was compared with their minimum inhibitory concentrations for AMC in strains of two serovars. We observed that the strains of the serovar O: 6, 30-6, 31 showed a similar relationship between the number of VFs...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: N SinghalM KumarJS Virdi Source Type: research

A study on gender-related differences in laboratory characteristics of dengue fever
A Chakravarti, P Roy, S Malik, O Siddiqui, P ThakurIndian Journal of Medical Microbiology 2016 34(1):82-84Studies have reported significant gender-related differences in serological tests for detection of NS1 antigen and IgM antibody used for diagnosing dengue fever. However, no such study has been undertaken in India though dengue fever is endemic in this country. Therefore, this study was planned to study the association of serological findings with gender in 700 patients suspected to be suffering from dengue fever in the Indian setting. Haematological parameters of seropositive patients were also studied. Seropositivity...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: A ChakravartiP RoyS MalikO SiddiquiP Thakur Source Type: research

Expression of cytokine-mRNA in peripheral blood mononuclear cell of human immunodeficiency virus-1 subtype C infected individuals with opportunistic viral infections from India (South)
J Sachithanandham, VV Ramalingam, J Raja, OC Abraham, SA Pulimood, R KannangaiIndian Journal of Medical Microbiology 2016 34(1):76-81Human immunodeficiency virus (HIV) disease progression is associated with a marked change in the level of plasma cytokines. The study reported here investigated the level of mRNA expression of different cytokines: Tumour necrosis factor-alpha (TNF-α), interferon (INF)-gamma, interleukin-10 (IL-10) and IL-21 in the peripheral blood mononuclear cell among the antiretroviral therapy naive subtype C HIV-1 infected individuals and normal healthy controls by real time polymerase chain reaction...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: J SachithanandhamVV RamalingamJ RajaOC AbrahamSA PulimoodR Kannangai Source Type: research

Current spectrum of oculomycosis in North India: A 5-year retrospective evaluation of clinical and microbiological profile
S Verma, V Sharma, A Kanga, R Sharma, A Angrup, K Mokta, A GargIndian Journal of Medical Microbiology 2016 34(1):72-75Oculomycosis is a major cause of visual impairment. Eye pain, redness, discharge, diminution and photophobia are presenting features. We collected corneal scraping, vitreous, aqueous fluids and conjunctival swabs after the slit-lamp examination. Ophthalmological findings were hypopyon, stromal congestion, conjunctival congestion and epithelial defect. Direct microscopy of 10% potassium hydroxide (KOH) wet mounts, gram staining, fungal and bacterial cultures were performed. Fungal isolates were obtained ...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: S VermaV SharmaA KangaR SharmaA AngrupK MoktaA Garg Source Type: research

Bacteriological profile and antibiotic sensitivity pattern of neonatal septicaemia in a rural tertiary care hospital in North India
Conclusion: Our study highlights the recent emergence of Gram-positive organisms as predominant cause of neonatal septicemia in this part of Sub-Himalayan region, along with the review of literature which shows similar results from North India and rest of the world too. Though Gram-negative bacteria still remain the main cause of mortality in neonatal septicemia, we want to dispel the common notion among practitioners that they are the predominant isolates in neonatal septicemia. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: S ThakurK ThakurA SoodS Chaudhary Source Type: research

Identification of opportunistic enteric parasites among immunocompetent patients with diarrhoea from Northern India and genetic characterisation of Cryptosporidium and Microsporidia
Conclusion: Opportunistic enteric parasitic infestation was more common among immunocompetent patients with diarrhoea than healthy subjects. Special staining as well as molecular methods are essential for appropriate diagnosis of these parasites. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: U GhoshalA DeyP RanjanS KhandujaV AgarwalUC Ghoshal Source Type: research

Cloning and molecular characterisation of resuscitation promoting factor-like gene from Mycobacterium avium subspecies avium
Conclusions: Identification, cloning, sequencing and expression of a rpf-like gene from M. avium suggest that RpfA is present in this species also, which might be involved in reactivation phenomenon in this high-risk pathogen. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: R KavithaR Verma Source Type: research

Virulence versus fitness determinants in Escherichia coli isolated from asymptomatic bacteriuria in healthy nonpregnant women
Conclusions: The expression of adhesins, haemolysin, aerobactin, and capsule synthesis gene were similar in two groups suggesting their role as fitness factors. ABU isolates were better biofilm producers, reflecting its importance in silent persistence. Serum resistance gene which was more expressed in cystitis isolates may represent virulence determinant. Genetic makeup of E. coli does not change much rather genes helping in survival and colonisation are expressed equally in ABU and cystitis isolates as opposed to phenotypic attenuation of those that helps in invasion or inflammation in ABU isolates. (Source: Indian Journ...
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: Sugandha SrivastavaJyotsna AgarwalBharti MishraRicha Srivastava Source Type: research

Prevalence, outcome and risk factor associated with vancomycin-resistant Enterococcus faecalis and Enterococcus faecium at a Tertiary Care Hospital in Northern India
Conclusions: Strategies to limit the nosocomial infection especially in patients on ventilator support can reduce VRE incidence and related mortality. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - January 15, 2016 Category: Microbiology Authors: A TripathiSK ShuklaA SinghKN Prasad Source Type: research