PD-1: A critical player and target for immune normalization
Immunology. 2024 Jan 25. doi: 10.1111/imm.13755. Online ahead of print.ABSTRACTImmune system imbalances contribute to the pathogenesis of several different diseases, and immunotherapy shows great therapeutic efficacy against tumours and infectious diseases with immune-mediated derivations. In recent years, molecules targeting the programmed cell death protein 1 (PD-1) immune checkpoint have attracted much attention, and related signalling pathways have been studied clearly. At present, several inhibitors and antibodies targeting PD-1 have been utilized as anti-tumour therapies. However, increasing evidence indicates that P...
Source: Immunology - January 25, 2024 Category: Allergy & Immunology Authors: Xuening Liu Alison Zhao Su Xiao Haohao Li Menghua Li Wei Guo Qiuju Han Source Type: research
The Neurobeachin-like 2 protein (NBEAL2) controls the homeostatic level of the ribosomal protein RPS6 in mast cells
Immunology. 2024 Jan 25. doi: 10.1111/imm.13756. Online ahead of print.ABSTRACTThe Beige and Chediak-Higashi (BEACH) domain-containing, Neurobeachin-like 2 (NBEAL2) protein is a molecule with a molecular weight of 300 kDa. Inactivation of NBEAL2 by loss-of-function mutations in humans as well as deletion of the Nbeal2 gene in mice results in functional defects in cells of the innate immune system such as neutrophils, NK-cells, megakaryocytes, platelets and of mast cells (MCs). To investigate the detailed function of NBEAL2 in murine MCs we generated MCs from wild type (wt) and Nbeal2-/- mice, and deleted Nbeal2 by CRISPR/C...
Source: Immunology - January 25, 2024 Category: Allergy & Immunology Authors: Philine Wegner Julia Drube Lisa Ziegler Birgit Strotmann Raphaela Marquardt Claudia K üchler Marco Groth Bernhard Nieswandt Nico Andreas Sebastian Drube Source Type: research
Chimerism of avian IgY-scFv and truncated IgG-Fc: A novel strategy in cross-species antibody generation and enhancement
In this study, engineered mammalian ΔFc domains (CH2, CH3, and intact Fc region) were fused with anti-canine parvovirus-like particles avian IgY-scFv to produce chimeric antibodies and expressed in the HEK293 cell expression system. The obtained scFv-CH2, scFv-CH3, and scFv-Fc can bind with antigen specifically and dose-dependently. Surface plasmon resonance investigation confirmed that scFv-CH2, scFv-CH3, and scFv-Fc had different degrees of binding to FcRn, with scFv-Fc showing the highest affinity. scFv-Fc had a significantly longer half-life in mice compared with the unfused scFv. The identified ΔFcs are promising fo...
Source: Immunology - January 22, 2024 Category: Allergy & Immunology Authors: Shikun Ge Alberto Carlos Pires Dias Xiaoying Zhang Source Type: research
Anti- and pro-inflammatory milieu differentially regulate differentiation and immune functions of oligodendrocyte progenitor cells
We examined the effects of pro-inflammatory cytokines, represented by interferon (IFN)-γ and tumour necrosis factor (TNF)-α, as well as anti-inflammatory cytokines, represented by interleukin (IL)-4 and IL-10, on OPC differentiation and immune characteristics. Using primary cultures, enzyme-linked immunosorbent assay and immunofluorescence stainings, we assessed differentiation capacity, phagocytic activity, major histocompatibility complex (MHC)-II expression, and cytokine secretion. We observed that the anti-inflammatory milieu (IL4 and IL10) reduced both OPC differentiation and immune functions. Conversely, exposure t...
Source: Immunology - January 20, 2024 Category: Allergy & Immunology Authors: Omri Zveik Ariel Rechtman Livnat Brill Adi Vaknin-Dembinsky Source Type: research
Sequential engagement of adhesion molecules and cytokine receptors impacts both piecemeal and anaphylactic degranulation of human basophils
Immunology. 2024 Jan 16. doi: 10.1111/imm.13754. Online ahead of print.ABSTRACTBasophils are rare granulocytes in circulation which home to tissues in a process depending on rolling, adhesion and cytokine exposure. However, it is still unclear how these steps affect basophil degranulation. Our aim was to imitate these processes associated with homing by sequential crosslinking of adhesion molecules and cytokine exposure and evaluate the effect on basophil piecemeal (PMD) and anaphylactic degranulation (AND). Blood donors with or without allergic asthma were recruited from an ongoing cohort study. Basophils were subjected t...
Source: Immunology - January 16, 2024 Category: Allergy & Immunology Authors: Ladan Mansouri Frida Kalm Sophia Bj örkander Erik Mel én Joachim Lundahl Anna Nopp Source Type: research
Prognostic biomarkers associated with immune checkpoint inhibitors in hepatocellular carcinoma
Immunology. 2024 Jan 12. doi: 10.1111/imm.13751. Online ahead of print.ABSTRACTThe treatment of hepatocellular carcinoma (HCC), particularly advanced HCC, has been a serious challenge. Immune checkpoint inhibitors (ICIs) are landmark drugs in the field of cancer therapy in recent years, which have changed the landscape of cancer treatment. In the field of HCC treatment, this class of drugs has shown good therapeutic prospects. For example, atezolizumab in combination with bevacizumab has been approved as first-line treatment for advanced HCC due to significant efficacy. However, sensitivity to ICI therapy varies widely amo...
Source: Immunology - January 12, 2024 Category: Allergy & Immunology Authors: Xiu Chen Liqiu Kou Xiaolu Xie Song Su Jun Li Yaling Li Source Type: research
Mycobacterium vaccae alleviates allergic airway inflammation and airway hyper-responsiveness in asthmatic mice by altering intestinal microbiota
Immunology. 2024 Jan 11. doi: 10.1111/imm.13750. Online ahead of print.ABSTRACTHost immunity can influence the composition of the gut microbiota and consequently affect disease progression. Previously, we reported that a Mycobacterium vaccae vaccine could ameliorate allergic inflammation in asthmatic mice by regulating inflammatory immune processes. Here, we investigated the anti-inflammatory effects of M. vaccae on allergic asthma via gut microbiota modulation. An ovalbumin (OVA)-induced asthmatic murine model was established and treated with M. vaccae. Gut microbiota profiles were determined in 18 BALB/c mice using 16S r...
Source: Immunology - January 11, 2024 Category: Allergy & Immunology Authors: Huan Xiao Li-Ting Fang An-Zhou Tang Hong-Liu Chen Mei-Li Xu Xiao-Shua Wei Guo-Dong Pang Chao-Qian Li Source Type: research
Macrophage expressed tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis progression
In this study, we have utilized various perturbations of the bleomycin mouse model of IPF including genetic knockout, RANKL inhibition, and macrophage adoptive transfer to further understand ACP5's role in pulmonary fibrosis. Genetic ablation of Acp5 decreased immune cell recruitment to the lungs and reduced the levels of hydroxyproline (reflecting extracellular matrix-production) as well as histological damage. Additionally, gene expression profiling of murine lung tissue revealed downregulation of genes including Ccl13, Mmp13, and Il-1α that encodes proteins specifically related to immune cell recruitment and macrophage...
Source: Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Jesper Bergwik Ravi Kiran Varma Bhongir M édea Padra Anna Adler Franziska Olm Pernilla L ång Sandra Lindstedt G öran Andersson Arne Egesten Lloyd Tanner Source Type: research
Macrophage expressed tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis progression
In this study, we have utilized various perturbations of the bleomycin mouse model of IPF including genetic knockout, RANKL inhibition, and macrophage adoptive transfer to further understand ACP5's role in pulmonary fibrosis. Genetic ablation of Acp5 decreased immune cell recruitment to the lungs and reduced the levels of hydroxyproline (reflecting extracellular matrix-production) as well as histological damage. Additionally, gene expression profiling of murine lung tissue revealed downregulation of genes including Ccl13, Mmp13, and Il-1α that encodes proteins specifically related to immune cell recruitment and macrophage...
Source: Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Jesper Bergwik Ravi Kiran Varma Bhongir M édea Padra Anna Adler Franziska Olm Pernilla L ång Sandra Lindstedt G öran Andersson Arne Egesten Lloyd Tanner Source Type: research
Macrophage expressed tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis progression
In this study, we have utilized various perturbations of the bleomycin mouse model of IPF including genetic knockout, RANKL inhibition, and macrophage adoptive transfer to further understand ACP5's role in pulmonary fibrosis. Genetic ablation of Acp5 decreased immune cell recruitment to the lungs and reduced the levels of hydroxyproline (reflecting extracellular matrix-production) as well as histological damage. Additionally, gene expression profiling of murine lung tissue revealed downregulation of genes including Ccl13, Mmp13, and Il-1α that encodes proteins specifically related to immune cell recruitment and macrophage...
Source: Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Jesper Bergwik Ravi Kiran Varma Bhongir M édea Padra Anna Adler Franziska Olm Pernilla L ång Sandra Lindstedt G öran Andersson Arne Egesten Lloyd Tanner Source Type: research
Macrophage expressed tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis progression
In this study, we have utilized various perturbations of the bleomycin mouse model of IPF including genetic knockout, RANKL inhibition, and macrophage adoptive transfer to further understand ACP5's role in pulmonary fibrosis. Genetic ablation of Acp5 decreased immune cell recruitment to the lungs and reduced the levels of hydroxyproline (reflecting extracellular matrix-production) as well as histological damage. Additionally, gene expression profiling of murine lung tissue revealed downregulation of genes including Ccl13, Mmp13, and Il-1α that encodes proteins specifically related to immune cell recruitment and macrophage...
Source: Immunology - January 5, 2024 Category: Allergy & Immunology Authors: Jesper Bergwik Ravi Kiran Varma Bhongir M édea Padra Anna Adler Franziska Olm Pernilla L ång Sandra Lindstedt G öran Andersson Arne Egesten Lloyd Tanner Source Type: research
Gut microbiota and intestinal immunity-A crosstalk in irritable bowel syndrome
Immunology. 2024 Jan 4. doi: 10.1111/imm.13749. Online ahead of print.ABSTRACTIrritable bowel syndrome (IBS), one of the most prevalent functional gastrointestinal disorders, is characterized by recurrent abdominal pain and abnormal defecation habits, resulting in a severe healthcare burden worldwide. The pathophysiological mechanisms of IBS are multi-factorially involved, including food antigens, visceral hypersensitivity reactions, and the brain-gut axis. Numerous studies have found that gut microbiota and intestinal mucosal immunity play an important role in the development of IBS in crosstalk with multiple mechanisms. ...
Source: Immunology - January 4, 2024 Category: Allergy & Immunology Authors: Yuxuan Chen Shuyan Feng Ying Li Chi Zhang Guanqun Chao Shuo Zhang Source Type: research
Gut microbiota and intestinal immunity-A crosstalk in irritable bowel syndrome
Immunology. 2024 Jan 4. doi: 10.1111/imm.13749. Online ahead of print.ABSTRACTIrritable bowel syndrome (IBS), one of the most prevalent functional gastrointestinal disorders, is characterized by recurrent abdominal pain and abnormal defecation habits, resulting in a severe healthcare burden worldwide. The pathophysiological mechanisms of IBS are multi-factorially involved, including food antigens, visceral hypersensitivity reactions, and the brain-gut axis. Numerous studies have found that gut microbiota and intestinal mucosal immunity play an important role in the development of IBS in crosstalk with multiple mechanisms. ...
Source: Immunology - January 4, 2024 Category: Allergy & Immunology Authors: Yuxuan Chen Shuyan Feng Ying Li Chi Zhang Guanqun Chao Shuo Zhang Source Type: research
Adenosine 2A receptor antagonists promote lymphocyte proliferation in sepsis by inhibiting Treg expression of PD-L1 in spleen
Immunology. 2023 Dec 30. doi: 10.1111/imm.13744. Online ahead of print.ABSTRACTThe spleen is essential for lymphocyte proliferation, which is associated to sepsis prognosis. Adenosine 2A receptor (A2AR) blocking promotes lymphocyte proliferation in sepsis, however the mechanism is uncertain. Our sepsis cecum ligation perforation model showed that blocking A2AR increased survival and CD4+ cell numbers in a spleen-dependent mechanism. The sequencing of the transcriptome of the spleen indicated alterations in the expression of genes involved in the control of lymphocyte proliferation by inhibiting A2AR, including a reduction ...
Source: Immunology - December 30, 2023 Category: Allergy & Immunology Authors: Teng Zhang Jie Zhao Ting Zheng Wei Fu Tao Ma Source Type: research
Circadian rhythm regulation in the immune system
Immunology. 2023 Dec 30. doi: 10.1111/imm.13747. Online ahead of print.ABSTRACTCircadian rhythms are a ubiquitous feature in nearly all living organisms, representing oscillatory patterns with a 24-h cycle that are widespread across various physiological processes. Circadian rhythms regulate a multitude of physiological systems, including the immune system. At the molecular level, most immune cells autonomously express clock-regulating genes, which play critical roles in regulating immune cell functions. These functions encompass migration, phagocytic activity, immune cell metabolism (such as mitochondrial structural funct...
Source: Immunology - December 30, 2023 Category: Allergy & Immunology Authors: Jun Ding Pengyu Chen Chunjian Qi Source Type: research
