TREM1/DAP12 based novel multiple chain CAR-T cells targeting DLL3 show robust anti-tumour efficacy for small cell lung cancer
In this study, we have developed a DLL3-TREM1/DAP12 CAR-T (DLL3-DT CAR-T) therapy, demonstrating comparable anti-tumour efficacy against SCLC cells in vitro. In murine xenograft and patient-derived xenograft models, DLL3-DT CAR-T cells exhibited a more robust tumour eradication efficiency than second-generation DLL3-BBZ CAR-T cells. Furthermore, we observed elevated memory phenotypes, induced durable responses, and activation under antigen-presenting cells in DLL3-DT CAR-T cells. Collectively, these findings suggest that DLL3-DT CAR-T cells may offer a novel and potentially effective therapeutic strategy for treating DLL3-...
Source: Immunology - March 12, 2024 Category: Allergy & Immunology Authors: Fengqi Nie Yuli Chen Yanming Hu Peng Huang Xuefei Shi Jingsheng Cai Mantang Qiu Enxiu Wang Kaihua Lu Ming Sun Source Type: research
Prevention and treatment of HPV-related cancer through a mRNA vaccine expressing APC-targeting antigen
In conclusion, this study described a therapeutic mRNA vaccine inducing strong anti-tumour immunity in peripheral and in tumour microenvironment, holding promising potential to treat HPV-induced cancer and to prevent cancer recurrence.PMID:38471664 | DOI:10.1111/imm.13777 (Source: Immunology)
Source: Immunology - March 12, 2024 Category: Allergy & Immunology Authors: Xiaoxuan Li Huiyan Wang Wujiang Lai Jinrong Liao Wenyu Mo Keke Huang Liqing He Xiaomei Liang Zhibin Yu Jiang Xu Xianwu Hua Fujun Hou Jun Ding William Wei-Guo Jia Kuan Zhang Yifeng Wang Source Type: research
Cooperative induction of CXCL chemokines by inflammatory cytokines and oxidized phospholipids
This study analysed in vitro pro-inflammatory activity of inflammatory cytokines (TNFα and IL-1β) in a combination with a lipid DAMP molecule, oxidized palmitoyl-arachidonoyl-phosphatidylcholine (OxPAPC). The study was performed on endothelial and monocytic cell lines. The cells were treated with different concentrations of TNFα or IL-1β, OxPAPC and their combinations, either in the presence or absence of drugs regulating inflammation. Pro-inflammatory effects of TNFα/IL-1β and OxPAPC were estimated by analysis of chemokines CXCL8, CXCL2 and CXCL3 by ELISA and RT-PCR. Toxicity was determined by analysis of metabolic ...
Source: Immunology - March 12, 2024 Category: Allergy & Immunology Authors: Alma Hodzic Bernd Gesslbauer Valery Bochkov Olga V Oskolkova Source Type: research
TREM1/DAP12 based novel multiple chain CAR-T cells targeting DLL3 show robust anti-tumour efficacy for small cell lung cancer
In this study, we have developed a DLL3-TREM1/DAP12 CAR-T (DLL3-DT CAR-T) therapy, demonstrating comparable anti-tumour efficacy against SCLC cells in vitro. In murine xenograft and patient-derived xenograft models, DLL3-DT CAR-T cells exhibited a more robust tumour eradication efficiency than second-generation DLL3-BBZ CAR-T cells. Furthermore, we observed elevated memory phenotypes, induced durable responses, and activation under antigen-presenting cells in DLL3-DT CAR-T cells. Collectively, these findings suggest that DLL3-DT CAR-T cells may offer a novel and potentially effective therapeutic strategy for treating DLL3-...
Source: Immunology - March 12, 2024 Category: Allergy & Immunology Authors: Fengqi Nie Yuli Chen Yanming Hu Peng Huang Xuefei Shi Jingsheng Cai Mantang Qiu Enxiu Wang Kaihua Lu Ming Sun Source Type: research
Prevention and treatment of HPV-related cancer through a mRNA vaccine expressing APC-targeting antigen
In conclusion, this study described a therapeutic mRNA vaccine inducing strong anti-tumour immunity in peripheral and in tumour microenvironment, holding promising potential to treat HPV-induced cancer and to prevent cancer recurrence.PMID:38471664 | DOI:10.1111/imm.13777 (Source: Immunology)
Source: Immunology - March 12, 2024 Category: Allergy & Immunology Authors: Xiaoxuan Li Huiyan Wang Wujiang Lai Jinrong Liao Wenyu Mo Keke Huang Liqing He Xiaomei Liang Zhibin Yu Jiang Xu Xianwu Hua Fujun Hou Jun Ding William Wei-Guo Jia Kuan Zhang Yifeng Wang Source Type: research
Omicron breakthrough infections after triple-dose inactivated COVID-19 vaccination: A comprehensive analysis of antibody and T-cell responses
This study longitudinally evaluated the immune response in individuals over a year after receiving three doses of an inactivated SARS-CoV-2 vaccine, focusing on reactions to Omicron breakthrough infections. From 63 blood samples of 37 subjects, results showed that the third booster enhanced the antibody response against Alpha, Beta, and Delta VOCs but was less effective against Omicron. Although antibody titres decreased post-vaccination, SARS-CoV-2-specific T-cell responses, both CD4+ and CD8+ , remained stable. Omicron breakthrough infections significantly improved neutralization against various VOCs, including Omicron. ...
Source: Immunology - March 10, 2024 Category: Allergy & Immunology Authors: Tiandan Xiang Xufeng Quan Hang Jia Hua Wang Boyun Liang Sumeng Li Xiaoyan Wang Huadong Li Xuemei Feng Lei Fan Ling Xu Tong Wang Shue Xiong Dongliang Yang Jia Liu Xin Zheng Source Type: research
Omicron breakthrough infections after triple-dose inactivated COVID-19 vaccination: A comprehensive analysis of antibody and T-cell responses
This study longitudinally evaluated the immune response in individuals over a year after receiving three doses of an inactivated SARS-CoV-2 vaccine, focusing on reactions to Omicron breakthrough infections. From 63 blood samples of 37 subjects, results showed that the third booster enhanced the antibody response against Alpha, Beta, and Delta VOCs but was less effective against Omicron. Although antibody titres decreased post-vaccination, SARS-CoV-2-specific T-cell responses, both CD4+ and CD8+ , remained stable. Omicron breakthrough infections significantly improved neutralization against various VOCs, including Omicron. ...
Source: Immunology - March 10, 2024 Category: Allergy & Immunology Authors: Tiandan Xiang Xufeng Quan Hang Jia Hua Wang Boyun Liang Sumeng Li Xiaoyan Wang Huadong Li Xuemei Feng Lei Fan Ling Xu Tong Wang Shue Xiong Dongliang Yang Jia Liu Xin Zheng Source Type: research
Flavonoid extracted from Epimedium attenuate cGAS-STING-mediated diseases by targeting the formation of functional STING signalosome
Immunology. 2024 Mar 7. doi: 10.1111/imm.13771. Online ahead of print.ABSTRACTHyperactivation of the cyclic-GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signalling pathway has been shown to be associated with the development of a variety of inflammatory diseases, and the discovery of an inhibitor of the cGAS-STING signalling pathway holds great promise in the therapeutic interventions. Epimedium flavonoid (EF), a major active ingredient isolated from the medicinal plant Epimedium, has been reported to have good anti-inflammatory activity, but its exact mechanism of action remains unclear. In the present s...
Source: Immunology - March 7, 2024 Category: Allergy & Immunology Authors: Yan Wang Guang Xu Jincai Wen Xiaomei Zhao Huanying Zhao Guiji Lv Yingjie Xu Ye Xiu Junjie Li Simin Chen Qing Yao Yuanyuan Chen Lina Ma Xiaohe Xiao Junling Cao Zhaofang Bai Source Type: research
Flavonoid extracted from Epimedium attenuate cGAS-STING-mediated diseases by targeting the formation of functional STING signalosome
Immunology. 2024 Mar 7. doi: 10.1111/imm.13771. Online ahead of print.ABSTRACTHyperactivation of the cyclic-GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signalling pathway has been shown to be associated with the development of a variety of inflammatory diseases, and the discovery of an inhibitor of the cGAS-STING signalling pathway holds great promise in the therapeutic interventions. Epimedium flavonoid (EF), a major active ingredient isolated from the medicinal plant Epimedium, has been reported to have good anti-inflammatory activity, but its exact mechanism of action remains unclear. In the present s...
Source: Immunology - March 7, 2024 Category: Allergy & Immunology Authors: Yan Wang Guang Xu Jincai Wen Xiaomei Zhao Huanying Zhao Guiji Lv Yingjie Xu Ye Xiu Junjie Li Simin Chen Qing Yao Yuanyuan Chen Lina Ma Xiaohe Xiao Junling Cao Zhaofang Bai Source Type: research
Flavonoid extracted from Epimedium attenuate cGAS-STING-mediated diseases by targeting the formation of functional STING signalosome
Immunology. 2024 Mar 7. doi: 10.1111/imm.13771. Online ahead of print.ABSTRACTHyperactivation of the cyclic-GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signalling pathway has been shown to be associated with the development of a variety of inflammatory diseases, and the discovery of an inhibitor of the cGAS-STING signalling pathway holds great promise in the therapeutic interventions. Epimedium flavonoid (EF), a major active ingredient isolated from the medicinal plant Epimedium, has been reported to have good anti-inflammatory activity, but its exact mechanism of action remains unclear. In the present s...
Source: Immunology - March 7, 2024 Category: Allergy & Immunology Authors: Yan Wang Guang Xu Jincai Wen Xiaomei Zhao Huanying Zhao Guiji Lv Yingjie Xu Ye Xiu Junjie Li Simin Chen Qing Yao Yuanyuan Chen Lina Ma Xiaohe Xiao Junling Cao Zhaofang Bai Source Type: research
Comparison of the oncolytic activity of a replication-competent and a replication-deficient herpes simplex virus 1
Immunology. 2024 Mar 5. doi: 10.1111/imm.13775. Online ahead of print.ABSTRACTIn 2015, the oncolytic herpes simplex virus 1 (HSV-1) T-VEC (talimogene laherparepvec) was approved for intratumoral injection in non-resectable malignant melanoma. To determine whether viral replication is required for oncolytic activity, we compared replication-deficient HSV-1 d106S with replication-competent T-VEC. High infectious doses of HSV-1 d106S killed melanoma (n = 10), head-and-neck squamous cell carcinoma (n = 11), and chondrosarcoma cell lines (n = 2) significantly faster than T-VEC as measured by MTT metabolic activity, while low do...
Source: Immunology - March 6, 2024 Category: Allergy & Immunology Authors: Georg Lindner Annika Walter Clara L Magnus Katharina Rosenhammer Bohdan Holoborodko Victoria Koch Sarah Hirsch Luis Grossmann Suqi Li David M Knipe Neal DeLuca Beatrice Schuler-Thurner Stefanie Gross Barbara Schwertner Martina Toelge Anette Rohrhofer Sabi Source Type: research
Comparison of the oncolytic activity of a replication-competent and a replication-deficient herpes simplex virus 1
Immunology. 2024 Mar 5. doi: 10.1111/imm.13775. Online ahead of print.ABSTRACTIn 2015, the oncolytic herpes simplex virus 1 (HSV-1) T-VEC (talimogene laherparepvec) was approved for intratumoral injection in non-resectable malignant melanoma. To determine whether viral replication is required for oncolytic activity, we compared replication-deficient HSV-1 d106S with replication-competent T-VEC. High infectious doses of HSV-1 d106S killed melanoma (n = 10), head-and-neck squamous cell carcinoma (n = 11), and chondrosarcoma cell lines (n = 2) significantly faster than T-VEC as measured by MTT metabolic activity, while low do...
Source: Immunology - March 6, 2024 Category: Allergy & Immunology Authors: Georg Lindner Annika Walter Clara L Magnus Katharina Rosenhammer Bohdan Holoborodko Victoria Koch Sarah Hirsch Luis Grossmann Suqi Li David M Knipe Neal DeLuca Beatrice Schuler-Thurner Stefanie Gross Barbara Schwertner Martina Toelge Anette Rohrhofer Sabi Source Type: research
Comparison of the oncolytic activity of a replication-competent and a replication-deficient herpes simplex virus 1
Immunology. 2024 Mar 5. doi: 10.1111/imm.13775. Online ahead of print.ABSTRACTIn 2015, the oncolytic herpes simplex virus 1 (HSV-1) T-VEC (talimogene laherparepvec) was approved for intratumoral injection in non-resectable malignant melanoma. To determine whether viral replication is required for oncolytic activity, we compared replication-deficient HSV-1 d106S with replication-competent T-VEC. High infectious doses of HSV-1 d106S killed melanoma (n = 10), head-and-neck squamous cell carcinoma (n = 11), and chondrosarcoma cell lines (n = 2) significantly faster than T-VEC as measured by MTT metabolic activity, while low do...
Source: Immunology - March 6, 2024 Category: Allergy & Immunology Authors: Georg Lindner Annika Walter Clara L Magnus Katharina Rosenhammer Bohdan Holoborodko Victoria Koch Sarah Hirsch Luis Grossmann Suqi Li David M Knipe Neal DeLuca Beatrice Schuler-Thurner Stefanie Gross Barbara Schwertner Martina Toelge Anette Rohrhofer Sabi Source Type: research
Methyl-supplemented nutrition delays the development of autoimmune disease in pristane-induced murine lupus
Immunology. 2024 Mar 2. doi: 10.1111/imm.13774. Online ahead of print.ABSTRACTThe aetiology and progression of systemic lupus erythematosus (SLE) resulted from a complex sequence of events generated both from genetic and epigenetic processes. In the current research, the effect of methyl-supplemented nutrition on the development of SLE was studied in the pristane-induced mouse model of the disease. The results clearly demonstrated decreased anti-dsDNA antibody and proteinuria levels, modulation of cytokines and protected renal structures in the group of treated mice. An additional increase in the DNA methylation of mouse B...
Source: Immunology - March 2, 2024 Category: Allergy & Immunology Authors: Silviya Bradyanova Iliyan Manoylov Gabriela Boneva Lidiya Kechidzhieva Andrey Tchorbanov Kalina Nikolova-Ganeva Source Type: research
Methyl-supplemented nutrition delays the development of autoimmune disease in pristane-induced murine lupus
Immunology. 2024 Mar 2. doi: 10.1111/imm.13774. Online ahead of print.ABSTRACTThe aetiology and progression of systemic lupus erythematosus (SLE) resulted from a complex sequence of events generated both from genetic and epigenetic processes. In the current research, the effect of methyl-supplemented nutrition on the development of SLE was studied in the pristane-induced mouse model of the disease. The results clearly demonstrated decreased anti-dsDNA antibody and proteinuria levels, modulation of cytokines and protected renal structures in the group of treated mice. An additional increase in the DNA methylation of mouse B...
Source: Immunology - March 2, 2024 Category: Allergy & Immunology Authors: Silviya Bradyanova Iliyan Manoylov Gabriela Boneva Lidiya Kechidzhieva Andrey Tchorbanov Kalina Nikolova-Ganeva Source Type: research
