O-022 Pro - FET: The future of IVF
Abstract textAssisted reproductive technology (ART) represents a relatively new but extremely dynamic field of medicine. Throughout its 40 year-old history, there has been a number of paradigm changes, all of which aimed to optimise the efficacy and safety of this technology. This presentation will cover all the scientific developments of the last few years that have now build the case for yet another paradigm change; the transition to predominantly frozen embryo transfers. Emerging knowledge on the effect of the supraphysiological levels of sex steroids, usually present during a stimulated cycle, on the probability of pre...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-777 Comparison of GnRH-a trigger and GnRH-a plus low-dose HCG trigger for high ovarian responders in IVF/ICSI: A retrospective study based on propensity score matching
This study demonstrates that GnRH-a alone can reduce the incidence of moderate-to-severe OHSS without harming embyro quality in women with high ovarian response. These findings need further prospective validations in hyperresponsive populations by multi-center, large-sample, randomized controlled studies.Trial registration numberN/A (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-767 Cumulative live birth rate after IVF - trend over time and the impact of blastocyst culture and vitrification
AbstractStudy questionHas cumulative live birth rate (CLBR) improved over time and which factors are associated with such an improvement?Summary answerDuring 2007-2017, CLBR per oocyte aspiration increased significantly (27.0 % to 36.3 %), in parallel with an increase in blastocyst transfer and cryopreservation by vitrification.What is known alreadyWhile it has been shown that live birth rate (LBR) per embryo transfer (ET) is higher for fresh blastocyst than for fresh cleavage stage embryo transfer, CLBR per oocyte aspiration, including one fresh ET and all subsequent frozen embryo transfers (FET), does not seem to differ ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-096 The ethics of stem cell-based embryo-like structures: a focus group study on the attitudes of Dutch professionals and lay citizens
AbstractStudy questionWhat are main themes guiding the attitudes of Dutch professionals and lay citizens with regard to the creation and research use of embryo-like structures?Summary answerThe attitudes of Dutch citizens indicate feelings of trust and distrust, distinct ‘embryo’ conceptualizations, competing moral values and beliefs, and criteria for adequate regulatory safeguards.What is known alreadyResearchers are hopeful that the creation and research use of so-called embryo-like structures (ELS), i.e., stem cell-based models that can mimic (parts of) early (human) embryogenesis, may provide a morally less controv...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-087 Embryos with higher mitochondrial DNA ratios show better clinical outcomes in single euploid embryo transfer
This study is a retrospective cohort study from 2016 to 2019, including 1465 single embryo transfer cycles.Participants/materials, setting, methodsThe involved embryos were biopsied on Day 5 or 6 and the mitochondrial DNA ratio of 1465 embryos was examined undergoing PGS/NGS. The mitochondrial DNA ratios were normalized for technical batch-to-batch variation. The mitochondrial DNA ratio between the implantation group and non-implantation group was statistically analyzed. Data were analyzed by the student ’s t-test for continuous variables and Chi-square test for categorical variables.Main results and the role of chanceTh...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-073 Perinatal outcomes of infants conceived using partner versus donor sperm - An analysis of singleton and twin pregnancies from the UK national dataset
AbstractStudy questionAre perinatal outcomes different in pregnancies conceived using donor sperm compared with those with partner sperm?Summary answerThe perinatal outcomes of singleton and twin pregnancies conceived with donor sperm are better when compared to those conceived with partner spermWhat is known alreadyThere has been a substantial increase in the use of donor sperm in the last 15 years across the world. A recent systematic review and meta-analysis has suggested that there is an increased risk of hypertensive disorders of pregnancy and small for gestational age babies from ART treatment using donor sperm compa...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –171 Automated oocyte and zygote denudation using a novel microfluidic device supervised by a computer vision algorithm
This study was conducted during 2020 – 2021.Participants/materials, setting, methodsWe developed a customized microfluidic biochip that exerts a particular fluid motion while avoiding egg entrapment within microfluidic channels. The denudation efficacy was established by subjectively scoring images of bovine oocytes after generating a continuous “Push& Pull ” fluid motion inside the biochip wells. A Computer Vision model was developed in parallel in order to optically assess denudation completion. The model used was a Pytorch implementation of Faster-RCNN with ImageNet pretrained weightsMain results and the role ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –170 The secretomy of embryo sex
AbstractStudy questionDoes the analysis of the metabolites of the embryonic culture medium can predict the sex of the embryo?Summary answerThe presence and quantity of some metabolites in the culture medium can predict the sex of the human embryos.What is known alreadyAdvances in analytical techniques for metabolomics have brought the possibility of better tools for the characterization of molecules. Embryonic metabolism can be used as a good indicator of viability, regardless of the morphology of the blastocysts, since differences were observed in the metabolic activities between the days of embryo development and in the ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –169 Does increasing the time interval between Oocyte-Retrieval and Oocyte-Denudation improve the results in ICSI cycles ?
AbstractStudy questionWhat should be the optimal time interval which elapses between oocyte retrieval and denudation followed by ICSI , for optimal results in ART cycles ?Summary answerOur study suggests that an optimum interval between oocyte retrieval and oocyte denudation followed by ICSI, leads to better results in ART cycles.What is known alreadyIt is widely accepted that the best timing for OPU is 34 –39 hours after ovulation trigger. Some studies suggest that preincubation time before ICSI can be beneficial when it comes to fertilization and pregnancy rates while late ICSI (fertilization) may have negative resul...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –168 RCT comparing the effect of Continuous ( Single Step ) embryo culture system versus a Sequential embryo culture system on the outcome of IVF/ICSI cycles
In conclusion, culture in single step media was associated with higher blastocyst formation rates compared to sequential media , suggesting that the single medium may provide better support to the developing embryo. The proportion of poor quality embryos was significantly higher in the sequential media group. Results indicate that embryo culture in continuous media could be as efficient as embryo culture in sequential media. A significant difference observed was the proportion of poor quality embryos on day 5 , which was significantly higher when the embryos were cultured in sequential media. Our results suggest that the t...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-088 Performance of a commercial artificial intelligence software for embryo selection (Embryoscope/KIDScore ™) on predicting biopsied and non-biopsied blastocyst clinical pregnancy according to score subgroups
AbstractStudy questionHow informative is the score grade of KIDScore version 3 for day 5 blastocyst for clinical pregnancy in biopsied and non-biopsied embryos?Summary answerPotential clinical pregnancy is predicable according to score grades (above 7.0), regardless the use of PGT-A, in blastocysts on day 5.What is known alreadyTime-lapse technology has promoted, along with the use of artificial intelligence (A.I.), a new spectrum of tools to improve embryo selection. Several software and algorithms have been launched in ART field in the last years, with the perspective of providing a substantial boost in IVF outcomes. KID...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-076 Neurodevelopmental morbidity in children born after ART: a Nordic register study from the Committee of Nordic ART and Safety (CoNARTaS) group
AbstractStudy questionDoes the risk of neurodevelopmental disorders differ between singletons born after various assisted reproductive techniques (ART) and spontaneous conception (SC) until young adulthood?Summary answerART children had a slightly increased rate of learning and motor functioning disorders, autism spectrum disorders (ASD), and ADHD and conduct disorders.What is known alreadyStudies on the impact of ART on offspring have reported both increased risk and comparable incidences of neurodevelopmental disorders between ART and SC offspring. The most studied neurodevelopmental disorders with ART are autism spectru...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –006 Simultaneous determination of bisphenol A and S in the samples of human seminal fluid
In this study we developed targeted analytical method based on liquid chromatography tandem mass (LC-MS/MS) detection to measure bisphenol A and S in seminal fluid samples obtained from IVF clinic. A total of 140 samples were analysed.Participants/materials, setting, methodsBPA and BPS was extracted from 140 seminal fluid samples using solvent extraction followed by preconcentration step. Samples were analyzed on Agilent 6495 Triple Quadrupole (Agilent Technologies, Santa Clara, CA) operating in the ESI-negative mode. Two MS/MS transitions were used for quantitative LC-MS/MS analyses. Chromatographic separation was achieve...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-209 The impact of sperm DNA fragmentation on pregnancy outcomes depends on oocyte dimorphisms
AbstractStudy questionDoes the impact of sperm DNA fragmentation (SDF) on Intracytoplasmic sperm injection (ICSI) outcomes depend on the presence of oocyte dimorphisms?Summary answerThere is a significant influence of oocyte quality on the impact of SDF on pregnancy outcomes.What is known alreadySperm DNA fragmentation has been associated with ICSI outcomes. DNA damage is commonly encountered in human spermatozoa and it has been widely accepted that the oocyte assumes responsibility for the repair and remodelling of both the maternal and paternal genomes during the oocyte-embryo transition. Indeed, spermatozoa with DNA dam...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-208 High sperm DNA fragmentation index negatively impacts embryo morphokinetics, but not embryo morphology and development rates: the importance of time-lapse imaging system
AbstractStudy questionCan time-lapse imaging (TLI) identify morphokinetic events impacted by high sperm DNA fragmentation index (DFI), irrespective of conventional morphological embryo assessment and development rate?Summary answerEmbryo morphokinetic parameters are negatively impacted by high DFI, whereas conventional morphological embryo assessment and blastocyst development rate are not related to DNA integrity.What is known alreadyPaternal genome activation occurs late in the embryo, and therefore, the negative impact of sperm factors on embryo development is more often observed in the outcomes of pregnancy, such as em...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
