Early metastatic colorectal cancers show increased tissue expression of miR-17/92 cluster members in the invasive tumor front
Accurate prediction of regional lymph node metastases (LNM) in endoscopically resected pT1 colorectal cancer (CRC) is crucial in treatment stratification for subsequent radical surgery. Several miRNAs have been linked to CRC invasion and metastasis, including the oncogenic miR-17/92 cluster, and expression levels might have predictive value in the risk assessment of early metastatic progression in CRC. We performed global miRNA microarray using tissue samples from the invasive front of pT1 CRC and investigated associations of the miR-17/92 cluster and presence of LNM. (Source: Human Pathology)
Source: Human Pathology - June 11, 2018 Category: Pathology Authors: Rikke Karlin Jepsen, Guy Wayne Novotny, Louise Laurberg Klarskov, Claus Heiner Bang-Bertelsen, Ida Trondhjem Haakansson, Anker Hansen, Ib Jarle Christensen, Lene Buhl Riis Ass, Estrid H øgdall Tags: Original Contributions Source Type: research
Androgen receptor expression is a favorable prognostic factor in triple-negative breast cancers
Androgen receptor (AR) expression is an emerging prognostic marker that has been observed in 25% to 75% of triple negative breast cancers (TNBCs) that lack estrogen receptor, progesterone receptor and HER2 overexpression. TNBCs are treated with AR-targeted therapies and standardized evaluation of AR expression may help guide patient management. Basal-like TNBCs are a subgroup of TNBCs defined by positive immunoreactivity for CK5/6 or EGFR that carry a worse prognosis. However, it's unclear whether basal-like TNBCs have a different rate of AR positivity or if AR expression is predictive of disease free survival in this pati...
Source: Human Pathology - June 11, 2018 Category: Pathology Authors: Tao Zuo, Parker Wilson, Ali Fuat Cicek, Malini Harigopal Tags: Original contribution Source Type: research
The Impact of the effectiveness of GATA3 as a prognostic factor in breast cancer
The transcription factor GATA3 plays a significant role in mammary gland development and differentiation. We analyzed expression of GATA3 in breast cancer (BC) cell lines and clinical specimens from BC patients in Taiwan. Semi-quantitative reverse transcriptase (RT)-polymerase chain reaction (PCR), quantitative real-time PCR carried out to determine the mRNA level of GATA3 from 241 pairs of matched tumor and adjacent normal tissues from anonymous female donors. GATA3 immunohistochemistry (IHC) staining and H-score were performed (n=25). (Source: Human Pathology)
Source: Human Pathology - June 11, 2018 Category: Pathology Authors: Fararjeh-Salah Abdul Fattah, Shih-Hsin Tu, Li-Ching Chen, Yun-Ru Liu, Yen-Kuang Lin, Hang-Lung Chang, Hui-Wen Chang, Chih-Hsiung Wu, Wendy-Hwang Verslues, Yuan-Soon Ho Tags: Original contribution Source Type: research
Desmoplastic non- infantile astrocytoma/Ganglioglioma: rare low-grade tumor with frequent BRAF V600E mutation
This study was carried out to evaluate the frequency of BRAF V600E mutation in DIA/DIG. All cases of DIA/DIGs diagnosed over 7years (2010–2016) were reviewed ret rospectively. The clinical, radiological and histopathological characteristics of these patients were evaluated along with immunohistochemical analysis for glial and neuronal markers. (Source: Human Pathology)
Source: Human Pathology - June 11, 2018 Category: Pathology Authors: Debajyoti Chatterjee, Chandni Garg, Navneet Singla, Bishan Dass Radotra Tags: Original contribution Source Type: research
Lymphomas with pseudo-double hit BCL6-MYC translocations due to t(3;8)(q27;q24) are associated with a germinal center immunophenotype, extranodal involvement, and frequent BCL2 translocations
High-grade B-cell lymphomas with MYC, BCL2, and/or BCL6 rearrangements, “double hit” or “triple hit” lymphomas (DTHL), are aggressive neoplasms associated with a poor prognosis. A t(3;8)(q27;q24) rarely occurs in B-cell lymphomas that results in a unique “pseudo-double hit” BCL6-MYC fusion, indistinguishable by interphase fluorescence in situ hybridization ( FISH) from more conventional DTHL with independent MYC and BCL6 translocations. Reports of t(3;8)(q27;q24) lymphomas are sparse, and to better characterize their pathologic, cytogenetic, and clinical features, 6 new cases from 2 institutions and 19 previous...
Source: Human Pathology - June 11, 2018 Category: Pathology Authors: Steven M. Johnson, Jayadev Manikkam Umakanthan, Ji Yuan, Yuri Fedoriw, R. Gregory Bociek, Kathleen Kaiser-Rogers, Jennifer N. Sanmann, Nathan D. Montgomery Tags: Original contribution Source Type: research
FOXP3-positive T-cell lymphomas in non-HTLV1 carriers include ALK-negative anaplastic large cell lymphoma: expanding the Spectrum of T-cell lymphomas with regulatory phenotype
We report 6 cases of T-cell lymphomas with Treg phenotype based on diffuse positivity for FOXP3 in tumor cells. The patients showed a median age of 56years with a male predominance. Sites of disease included lymph nodes (4), skin (2), subcutaneous tissue (1) and bone marrow (1). All cases showed monomorphic large cells, some with Hodgkin-like or anaplastic cells. All cases expressed pan T cell markers and lacked cytotoxic markers; one case showed diffuse PD1 staining. (Source: Human Pathology)
Source: Human Pathology - June 10, 2018 Category: Pathology Authors: Cristiane R. Ferreira, Shuchun Zhao, Malaya K. Sahoo, Benjamin Pinsky, Jenna Weber, Luis A.P.C. Lage, Juliana Pereira, Maria C.N. Zerbini, Yasodha Natkunam Tags: Original Contributions Source Type: research
Myosin 5a regulates tumor migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma: utility as a prognostic factor
In this study, we confirmed the expression of myosin 5a in ESCC surgical specimens and cell lines, revealing its correlation with tumor invasion, migration, patient prognosis, and expression of EMT-related proteins. (Source: Human Pathology)
Source: Human Pathology - June 10, 2018 Category: Pathology Authors: Naomi Sato, Fumiyoshi Fujishima, Yasuhiro Nakamura, Yayoi Aoyama, Yoshiaki Onodera, Yohei Ozawa, Ken Ito, Hirotaka Ishida, Takashi Kamei, Mika Watanabe, Hironobu Sasano Tags: Original Contributions Source Type: research
Clinicopathological, Immunohistochemical and molecular correlation of neural crest transcription factor SOX10 expression in triple negative breast carcinoma
The transcription factor SOX10 mediates the differentiation of neural crest –derived cells, and SOX10 by immunohistochemistry(IHC) is used primarily for the diagnosis of melanoma. SOX10 expression has been previously documented in benign breast myoepithelial cells. However there is limited literature on its expression in triple negative breast carcinoma(TNBC). The aim was to study the clinical, pathologic and molecular profiles of SOX10+ tumors in TNBC. Tissue microarrays of TNBC were evaluated for SOX10 expression in 48cases. (Source: Human Pathology)
Source: Human Pathology - June 9, 2018 Category: Pathology Authors: Aparna Harbhajanka, Satyapal Chahar, Kristy Miskimen, Paula Silverman, Lyndsay Harris, Nicole Williams, Vinay Varadan, Hannah Gilmore Tags: Original contribution Source Type: research
Insulinoma-associated protein 1 is a novel sensitive and specific marker for small cell carcinoma of the prostate
In this study, we analyzed the expression of INSM in three high-throughput RNA sequencing data sets and performed INSM1 immunohistochemistry on a large series of prostatic SCCs and nonneuroendocrine prostate tissues. (Source: Human Pathology)
Source: Human Pathology - June 6, 2018 Category: Pathology Authors: Wei Xue, Zhixiang Xin, Yong Zhang, Zhou Jiang, Ling Zhao, Liancheng Fan, Yanqing Wang, Shaowei Xie, Xun Shangguan, Yinjie Zhu, Jiahua Pan, Qiang Liu, Yiran Huang, Baijun Dong Tags: Original Contributions Source Type: research
Evaluation of tumor-infiltrating lymphocytes using routine H & E slides predicts patient survival in resected non-small cell lung cancer
The presence of tumor-infiltrating lymphocytes (TILs) positively impacts the outcome of non-small cell lung cancer (NSCLC) patients. Most previous studies have assessed TILs using different immunohistochemical assays. The purpose of this study was to develop and validate a histopathological scoring model for the assessment of TILs in whole tissue hematoxylin and eosin (H&E)-stained section slides of NSCLC patients and to evaluate the model in an immunoscore setting. Therefore, TIL was evaluated manually on H&E slides from 537 surgical specimens of primary resected stage I-III NSCLC patients. (Source: Human Pathology)
Source: Human Pathology - June 6, 2018 Category: Pathology Authors: Mehrdad Rakaee, Thomas K. Kilvaer, Stig Manfred Dalen, Elin Richardsen, Erna-Elise Paulsen, Sigurd M. Hald, Samer Al-Saad, Sigve Andersen, Tom Donnem, Roy M. Bremnes, Lill-Tove Busund Tags: Original Contributions Source Type: research
In situ analysis of mTORC1/2 and cellular metabolism-related proteins in human Lymphangioleiomyomatosis
Lymphangioleiomyomatosis (LAM) is a rare progressive cystic lung disease with features of a low-grade neoplasm. It is primarily caused by mutations in TSC1 or TSC2 genes. Sirolimus, an inhibitor of mTOR complex 1 (mTORC1), slows down disease progression in some, but not all patients. Hitherto, other potential therapeutic targets such as mTOR complex 2 (mTORC2) and various metabolic pathways have not been investigated in human LAM tissues. The aim of this study was to assess activities of mTORC1, mTORC2 and various metabolic pathways in human LAM tissues through analysis of protein expression. (Source: Human Pathology)
Source: Human Pathology - June 6, 2018 Category: Pathology Authors: Ildiko Krencz, Anna Sebestyen, Judit Papay, Andras Jeney, Zoltan Hujber, Charles D. Burger, Cesar A. Keller, Andras Khoor Tags: Original Contributions Source Type: research
Evaluation of miRNAs expression in medullary thyroid carcinoma tissue samples: miR-34a and miR-144 as promising overexpressed markers in MTC
Medullary Thyroid Carcinoma (MTC) is a rare neoplasia derived from neural parafollicular C cells. MicroRNAs (miRNAs) are small regulatory RNAs with essential roles in the biology of cancers such as MTC and can be applied as diagnostic markers. According to previous studies, miR-144 and miR-34 and their two oncogenes target, mammalian target of rapamycin (mTOR) and AXL receptor tyrosine kinase (AXL), were selected for further investigations in our study. Thirty MTC samples as well as thirty adjacent normal thyroid tissues were applied in this study including 28 Formalin-Fixed Paraffin-Embedded (FFPE) and 2 fresh-frozen MTC ...
Source: Human Pathology - June 6, 2018 Category: Pathology Authors: Noushin Shabani, Javad Razaviyan, Mahdi Paryan, Seyed Mohammad Tavangar, Fereidoun Azizi, Samira Mohammadi-Yeganeh, Mehdi Hedayati Tags: Original Contributions Source Type: research
Epstein - Barr virus - associated lymphoid proliferations, a 2018 update
Epstein-Barr virus (EBV) has been linked to many human neoplasms including hematopoietic, epithelial, and mesenchymal tumors. Since our original review of EBV-associated lymphoproliferative disorders in 2007 (1), many advances and developments have been reported. In this review, we will examine the recent advances in EBV-associated lymphoid/ histiocytic proliferations, dividing them into reactive, B cell, T/NK cell, immunodeficiency-related, and histiocytic/dendritic cell proliferations. (Source: Human Pathology)
Source: Human Pathology - June 6, 2018 Category: Pathology Authors: Sherif A. Rezk, Xiaohui Zhao, Lawrence M. Weiss Tags: Perspectives in pathology Source Type: research
B7H4 is associated with stemness and cancer progression in esophageal squamous cell carcinoma
In this study, we have assessed the expression of B7H4 and cancer stemness proteins in 156 paraffin embedded ESCC tissue samples using immunohistochemistry as well as in ESCC cell lines using western blotting and immunofluorescence imaging. (Source: Human Pathology)
Source: Human Pathology - June 6, 2018 Category: Pathology Authors: Longzhen Piao, Zhaoting Yang, Jiajun Jin, Weidong Ni, Wenbo Qi, Yanhua Xuan Tags: Original Contributions Source Type: research
Micropapillary urothelial carcinoma: evaluation of HER2 status and immunohistochemical characterization of the molecular subtype
In this study, we focused on micropapillary urothelial carcinoma (MPUC), an aggressive, histomorphologically defined rare variant. Apart from genetic alterations shared with conventional UBC alterations of the HER2 gene have been reported in higher frequencies. However, only small cohorts of MPUCs have been analyzed and the real impact is still unclear. We collected a cohort of 94 MPUCs and immunohistochemically tested HER2, basal (CD44, CK5, EGFR, p63) and luminal (CD24, FOXA1, GATA3, CK20) markers to allocate MPUC to a molecular subtype. (Source: Human Pathology)
Source: Human Pathology - June 6, 2018 Category: Pathology Authors: Ulrike Zinnall, Veronika Weyerer, Eva Comp érat, Philippe Camparo, Nadine T. Gaisa, Ruth Knuechel-Clarke, Aurel Perren, Alessandro Lugli, Marieta Toma, Gustavo Baretton, Glen Kristiansen, Ralf M. Wirtz, Liang Cheng, Bernd Wullich, Robert Stoehr, Arndt Ha Tags: Original contribution Source Type: research
