Evaluating agreement, histological features and relevance of separating pleomorphic and florid lobular carcinoma-in-situ subtypes
Morphological variants of lobular carcinoma in situ (LCIS) include classical- (CLCIS), pleomorphic- (PLCIS) and florid-type (FLCIS). Treatment guidelines suggest managing PLCIS and FLCIS like ductal carcinoma in situ (DCIS); therefore accurate identification of LCIS subtypes is critical. However significance of separating PLCIS from FLCIS is not clear. Also inter-observer agreement in identifying LCIS subtypes, using contemporary criteria, is not known. We aimed to evaluate inter-observer agreement amongst breast pathologists in diagnosing LCIS subtypes and use the agreement data to justify LCIS classification for manageme...
Source: Human Pathology - May 9, 2018 Category: Pathology Authors: Kamaljeet Singh, Cherie Paquette, Elizabeth T Kalife, Yihong Wang, Shamlal Mangray, M Ruhul Quddus MD MPhil, Margaret M. Steinhoff Tags: Original contribution Source Type: research

The implications of TrkA and MET aberrations in de novo salivary duct carcinoma
In this study, the recently identified receptor tyrosine kinases MET and tropomyosin-receptor kinase (Trk) were investigated as potential therapeutic targets. A total of 28 consecutive, surgically resected, de novo SDC cases were selected after evaluating histology and immunohistochemical expression of androgen receptor. (Source: Human Pathology)
Source: Human Pathology - May 9, 2018 Category: Pathology Authors: Hyang Joo Ryu, Yoon Woo Koh, Sun Och Yoon Tags: Original Contributions Source Type: research

Next-generation sequencing analysis for gastric adenocarcinoma with enteroblastic differentiation: emphasis on the relationship with hepatoid adenocarcinoma
In this study, we employed next-generation sequencing (NGS) to establish a molecular/clinicopathological concept of GAED and clarify whether these two tumors should be grouped together in one category. (Source: Human Pathology)
Source: Human Pathology - May 8, 2018 Category: Pathology Authors: Yoichi Akazawa, Tsuyoshi Saito, Takuo Hayashi, Yuka Yanai, Sho Tsuyama, Keisuke Akaike, Yoshiyuki Suehara, Fumiyuki Takahashi, Kazuya Takamochi, Hiroya Ueyama, Takashi Murakami, Sumio Watanabe, Akihito Nagahara, Takashi Yao Tags: Original contribution Source Type: research

H3 K27M –mutant diffuse midline gliomas in different anatomical locations
The histone H3 K27M mutation has been frequently reported in most diffuse midline gliomas. However, the relationship between the H3 K27M mutation and clinical outcomes of gliomas from different anatomical locations is still not fully understood. A total of 120 patients with diffuse midline gliomas were selected for this retrospective observational study. The status of H3 K27M, ATRX, TP53, and IDH was evaluated using immunohistochemistry and Sanger sequencing. Of the 120 patients aged from 4 to 76 years (median, 27 years), 61 (50.8%) were harboring the H3 K27M mutation. (Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Authors: Leiming Wang, Zhuo Li, Ming Zhang, Yueshan Piao, Li Chen, Huiying Liang, Yukui Wei, Zeliang Hu, Lihong Zhao, Lianghong Teng, Dehong Lu Tags: Original contribution Source Type: research

Histology of colorectal adenocarcinoma with double somatic mismatch-repair mutations is indistinguishable from those caused by Lynch syndrome
Lynch syndrome (LS) is the most common form of hereditary colon cancer. Germline mutations in the mismatch-repair (MMR) genes MLH1, MSH2 (EPCAM), MSH6, and PMS2, followed by a second hit to the remaining allele, lead to cancer development. Universal tumor screening for LS is routinely performed on colon cancer, and screening has identified patients with unexplained MMR deficiency that lack MLH1 methylation and a germline mutation. Tumor sequencing has since identified double somatic (DS) mutations in the MMR gene corresponding with the absent protein in 69% of these patients. (Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Authors: Jessica A. Hemminger, Rachel Pearlman, Sigurdis Haraldsdottir, Deborah Knight, Jon Gunnlaugur Jonasson, Colin C. Pritchard, Heather Hampel, Wendy L. Frankel Tags: Original contribution Source Type: research

Masthead
(Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Source Type: research

Table of Contents
(Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Source Type: research

Editorial Board
(Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Source Type: research

In This Issue
(Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Source Type: research

H3 K27 M-mutant diffuse midline gliomas in different anatomical locations
The histone H3 K27 M mutation has been frequently reported in the majority of diffuse midline gliomas. However, the relationship between the H3 K27 M mutation and clinical outcomes of gliomas from different anatomical locations is still not fully understood. A total of 120 patients with diffuse midline gliomas were selected for this retrospective observational study. The status of H3 K27 M, ATRX, TP53 and IDH was evaluated using immunohistochemistry and Sanger sequencing. Of the 120 cases aged from 4 to 76years (median=27years), 61 (50.8%) were harboring the H3 K27 M mutation. (Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Authors: Leiming Wang, Zhuo Li, Ming Zhang, Yueshan Piao, Li Chen, Huiying Liang, Yukui Wei, Zeliang Hu, Lihong Zhao, Lianghong Teng, Dehong Lu Source Type: research

Clonality analysis of multifocal ipsilateral breast carcinomas using X-chromosome inactivation patterns
The definition of multifocal breast cancer is ambiguous, and its incidence varies depending on the definition and detection methods. Multifocal breast cancers either have the same clonal origin or arise from completely distinct progenitor cells. The current American Joint Committee on Cancer Staging system and College of American Pathologists breast tumor guidelines state that only the largest tumor needs to be staged and studied immunohistochemically, on the assumption that they are of the same origin. (Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Authors: Hayeon Kim, Chung-Yeul Kim, Kyong Hwa Park, Aeree Kim Source Type: research

Histology of colorectal adenocarcinoma with double somatic mismatch repair mutations is indistinguishable from those caused by lynch syndrome
Lynch syndrome (LS) is the most common form of hereditary colon cancer (CRC). Germline mutations in the mismatch repair (MMR) genes MLH1, MSH2 (EPCAM), MSH6, and PMS2, followed by a second hit to the remaining allele leads to cancer development. Universal tumor screening for LS is routinely performed on CRC, and screening has identified patients with unexplained MMR deficiency that lack MLH1 methylation and a germline mutation. Tumor sequencing has since identified double somatic (DS) mutations in the MMR gene corresponding with the absent protein in 69% of these patients. (Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Authors: Jessica A. Hemminger, Rachel Pearlman, Sigurdis Haraldsdottir, Deborah Knight, Jon Gunnlaugur Jonasson, Colin C. Pritchard, Heather Hampel, Wendy L. Frankel Tags: Original contribution Source Type: research

Central xanthoma of the jaw in association with Noonan syndrome
We present a case of a 15year-old-male with NS who presented with a radiolucent lesion of the mandible that on excision, was found to be a central xanthoma. (Source: Human Pathology)
Source: Human Pathology - May 1, 2018 Category: Pathology Authors: Nicholas J. Olson, Rocco R. Addante, Francine B. de Abreu, Vincent A. Memoli Source Type: research

Frozen section evaluation via dynamic real-time nonrobotic telepathology system in a university cancer center by resident/faculty cooperation team
Frozen section telepathology interpretation experience has been largely limited to practices with locations significantly distant from one another with sporadic need for frozen section diagnosis. In 2010, we established a real-time nonrobotic telepathology system in a very active cancer center for daily frozen section service. Herein, we evaluate its accuracy compared to direct microscopic interpretation performed in the main hospital by the same faculty and its cost-efficiency over a 1-year period. (Source: Human Pathology)
Source: Human Pathology - April 30, 2018 Category: Pathology Authors: Aram Vosoughi, Paul Taylor Smith, Joseph A. Zeitouni, Gregori M. Sodeman MSc, Merce Jorda, Carmen Gomez-Fernandez, Monica Garcia-Buitrago, Atousa Ordobazari, Carol K. Petito, Jennifer R. Chapman, German Campuzano-Zuluaga, Andrew E. Rosenberg, Oleksandr N. Tags: Original contribution Source Type: research

Non –small cell lung cancers with isocitrate dehydrogenase 1 or 2 (IDH1/2) mutations
In this study, we examine the prevalence and features of non–small cell lung cancers (NSCLC) with IDH1/2 mutations. From May 2013 to March 2017, 800 lung cancer samples were successfully sequenced for somatic mutations on the Ion Torrent PGM with the 50-gene AmpliSeq Cancer Hotspot Panel v2 on the Ion Torrent PGM (318 chip). (Source: Human Pathology)
Source: Human Pathology - April 30, 2018 Category: Pathology Authors: Laura N. Toth, Francine B. de Abreu, Laura J. Tafe Tags: Original contribution Source Type: research