Genes, Vol. 15, Pages 249: De Novo Genome Assembly of the Whitespot Parrotfish (Scarus forsteni): A Valuable Scaridae Genomic Resource
In this study, a genome of S. forsteni was assembled de novo through using Illumina and Nanopore sequencing. The 1.71-Gb genome of S. forsteni, was assembled into 544 contigs (assembly level: contig). It exhibited an N50 length of 17.97 Mb and a GC content percentage of 39.32%. Our BUSCO analysis revealed that the complete protein of the S. forsteni genome had 98.10% integrity. Combined with structure annotation data, 34,140 (74.81%) genes were functionally annotated out of 45,638 predicted protein-coding genes. Upon comparing the genome size and TE content of teleost fishes, a roughly linear relationship was observed betw...
Source: Genes - February 17, 2024 Category: Genetics & Stem Cells Authors: Yu Liang Lin Xian Jinmin Pan Kecheng Zhu Huayang Guo Baosuo Liu Nan Zhang Yan Ou-Yang Qin Zhang Dianchang Zhang Tags: Article Source Type: research
Genes, Vol. 15, Pages 248: Spatiotemporal Expression and Haplotypes Identification of KRT84 Gene and Their Association with Wool Traits in Gansu Alpine Fine-Wool Sheep
In this study, we analysed the expression, localization, and polymorphism of the K84 gene (KRT84) in Gansu Alpine Fine-wool sheep using immunofluorescence, RT-qPCR, and PARMS (Penta-primer amplification refractory mutation system). Haplotypes of KRT84 were also constructed and their relationship with wool traits analysed. It was revealed that KRT84 was highly expressed in hair follicles, including the inner root sheath, outer root sheath, and hair medulla and at all six lamb ages investigated from 1 to 270 days of age. Three SNPs were detected in KRT84 exon 1, and they formed three haplotypes (named H1, H2, and H3) and six...
Source: Genes - February 16, 2024 Category: Genetics & Stem Cells Authors: Xueqin Yu Shaobin Li Huitong Zhou Fangfang Zhao Jiang Hu Jiqing Wang Xiu Liu Mingna Li Zhidong Zhao Zhiyun Hao Bingang Shi Jon G. H. Hickford Tags: Article Source Type: research
Genes, Vol. 15, Pages 244: miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure
Keun Kim
The primary goal of this investigation was to identify mRNA targets affected by dysregulated miRNAs in RIF. This was accomplished by comprehensively analyzing mRNA and miRNA expression profiles in two groups: female subjects with normal reproductive function (control, n = 5) and female subjects experiencing recurrent implantation failure (RIF, n = 5). We conducted transcriptome sequencing and small RNA sequencing on endometrial tissue samples from these cohorts. Subsequently, we validated a selection of intriguing findings using real-time PCR with samples from the same cohort. In total, our analysis revealed ...
Source: Genes - February 15, 2024 Category: Genetics & Stem Cells Authors: Sung Hwan Cho Young Myeong Kim Hui Jeong An Ji Hyang Kim Nam Keun Kim Tags: Article Source Type: research
Genes, Vol. 15, Pages 245: When Livestock Genomes Meet Third-Generation Sequencing Technology: From Opportunities to Applications
ngwei Zhang
Third-generation sequencing technology has found widespread application in the ge (Source: Genes)
Source: Genes - February 15, 2024 Category: Genetics & Stem Cells Authors: Xinyue Liu Junyuan Zheng Jialan Ding Jiaxin Wu Fuyuan Zuo Gongwei Zhang Tags: Review Source Type: research
Genes, Vol. 15, Pages 246: Homozygous CNP Mutation and Neurodegeneration in Weimaraners: Myelin Abnormalities and Accumulation of Lipofuscin-like Inclusions
Martin L. Katz
A progressive neurological disorder was observed in a male neutered Weimaraner. Clinical signs included fecal incontinence, lethargy, moderate paraparesis, proprioceptive pelvic limb ataxia, falling, cognitive decline, incoordination, decreased interest in food, changes in posture, and episodes of trance-like behavior. Neurologic signs were first observed at approximately 4 years, 10 months of age and progressed slowly. Magnetic resonance imaging showed generalized brain atrophy with areas of white matter pathology. Humane euthanasia was elected at 6 years, 7 months of age due to increasing severity o...
Source: Genes - February 15, 2024 Category: Genetics & Stem Cells Authors: Stefan H. Keller Gary S. Johnson Garrett Bullock Tendai Mhlanga-Mutangadura Malte Schwartz Savannah G. Pattridge Juyuan Guo Gregg D. Kortz Martin L. Katz Tags: Article Source Type: research
