Genes, Vol. 15, Pages 292: Translation of Data from Animal Models of Cancer to Immunotherapy of Breast Cancer and Chronic Lymphocytic Leukemia
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The field of clinical oncology has been revolutionized over the past decade with the introduction of many new immunotherapies the existence of which have depended to a large extent on experimentation with both in vitro analysis and the use of various animal models, including gene-modified mice. The discussion below will review my own laboratory's studies, along with those of others in the field, on cancer immunotherapy. Our own studies have predominantly dwelt on two models of malignancy, namely a solid tumor model (breast cancer) and lymphoma. The data from our own laboratory, and that of other scientists, high...
Source: Genes - February 25, 2024 Category: Genetics & Stem Cells Authors: Reginald Gorczynski Tags: Article Source Type: research
Genes, Vol. 15, Pages 290: Drug Repurposing and Lysosomal Storage Disorders: A Trick to Treat
We present a comprehensive analysis of existing drugs, their repurposing potential, and their clinical implications in the context of LSDs, highlighting the necessity of mutation-specific approaches. Our review systematically explores the landscape of drug repositioning as a means to enhance LSDs therapies. The findings advocate for the strategic repositioning of drugs, accentuating its role in expediting the discovery of effective treatments. We conclude that drug repurposing represents a viable pathway for accelerating therapeutic discovery for LSDs, emphasizing the need for the careful evaluation of drug efficacy and to...
Source: Genes - February 25, 2024 Category: Genetics & Stem Cells Authors: Bruno Hay Mele Federica Rossetti Maria Vittoria Cubellis Maria Monticelli Giuseppina Andreotti Tags: Review Source Type: research
Genes, Vol. 15, Pages 293: A Scalable and Robust Chloroplast Genotyping Solution: Development and Application of SNP and InDel Markers in the Maize Chloroplast Genome
In this study, we performed next-generation sequencing of 176samples, and the maize cultivars represented five distinct groups. A total of 89 single nucleotide polymorphisms (SNPs) and 11 insertion/deletion polymorphisms (InDels) were identified. To enable high-throughput detection, we successfully amplified and confirmed 49 SNP and InDel markers, which were defined as a Varietal Chloroplast Panel (VCP) using the Kompetitive Allele Specific PCR (KASP). The specific markers provided a valuable tool for identifying chloroplast groups. The verification experiment, focusing on the identification of reciprocal cross hybrids and...
Source: Genes - February 25, 2024 Category: Genetics & Stem Cells Authors: Rui Wang Yang Yang Hongli Tian Hongmei Yi Liwen Xu Yuanda Lv Jianrong Ge Yikun Zhao Lu Wang Shiliang Zhou Fengge Wang Tags: Article Source Type: research
Genes, Vol. 15, Pages 295: Lignin Biosynthesis and Its Diversified Roles in Disease Resistance
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Lignin is complex, three-dimensional biopolymer existing in plant cell wall. Lignin biosynthesis is increasingly highlighted because it is closely related to the wide applications in agriculture and industry productions, including in pulping process, forage digestibility, bio-fuel, and carbon sequestration. The functions of lignin in planta have also attracted more attentions recently, particularly in plant defense response against different pathogens. In this brief review, the progress in lignin biosynthesis is discussed, and the lignin’s role in disease resistance are thoroughly elucidated. This issue...
Source: Genes - February 25, 2024 Category: Genetics & Stem Cells Authors: Qing-Hu Ma Tags: Review Source Type: research
Genes, Vol. 15, Pages 294: Genome-Wide Association Study Revealed Putative SNPs and Candidate Genes Associated with Growth and Meat Traits in Japanese Quail
Griffin
Johann Sölkner
Gottfried Brem
John C. McEwan
Rudiger Brauning
Natalia A. Zinovieva
The search for SNPs and candidate genes that determine the manifestation of major selected traits is one crucial objective for genomic selection aimed at increasing poultry production efficiency. Here, we report a genome-wide association study (GWAS) for traits characterizing meat performance in the domestic quail. A total of 146 males from an F2 reference population resulting from crossing a fast (Japanese) and a slow (Texas White) growing breed were examined. Using the genotyping-by-sequencing technique, genomic data...
Source: Genes - February 25, 2024 Category: Genetics & Stem Cells Authors: Natalia A. Volkova Michael N. Romanov Alexandra S. Abdelmanova Polina V. Larionova Nadezhda Yu. German Anastasia N. Vetokh Alexey V. Shakhin Ludmila A. Volkova Alexander A. Sermyagin Dmitry V. Anshakov Vladimir I. Fisinin Darren K. Griffin Johann S ölkne Tags: Article Source Type: research
Genes, Vol. 15, Pages 289: Heat-Resistant Inbred Lines Coordinate the Heat Response Gene Expression Remarkably in Maize (Zea mays L.)
ua Chen
High temperatures are increasingly becoming a prominent environmental factor accelerating the adverse influence on the growth and development of maize (Zea mays L.). Therefore, it is critical to identify the key genes and pathways related to heat stress (HS) tolerance in maize. Great challenges have been faced in dissecting genetic mechanisms and uncovering master genes for HS tolerance. Here, Z58D showed more thermotolerance than AF171 at the seedling stage with a lower wilted leaf rate and H2O2 accumulation under HS conditions. Transcriptomic analysis identified 3006 differentially expressed genes (DEGs) in A...
Source: Genes - February 25, 2024 Category: Genetics & Stem Cells Authors: Ming Xue Xiaoyue Han Luyao Zhang Saihua Chen Tags: Article Source Type: research
Genes, Vol. 15, Pages 283: Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, Helobdella austinensis
In this study, we focused on a narrow developmental time window of ~6–8 h, comprising stages just prior to and immediately following zygote deposition. Employing RNA-Seq methodology, we identified differentially expressed transcripts at this fundamental ontogenic boundary, known as the maternal-to-zygotic transition (MZT). Gene expression changes were characterized by the massive degradation of maternal RNAs (~45%) coupled with the rapid transcription of ~5000 zygotic genes (~20% of the genome) in the first mitotic cell cycle. The latter transcripts encoded a mixture of cell maintenance and regulatory protein...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Samuel Hsaio Naim Saglam David Morrow Daniel H. Shain Tags: Article Source Type: research
Genes, Vol. 15, Pages 284: Whole Genome Sequencing and Comparative Genomics of Six Staphylococcus schleiferi and Staphylococcus & nbsp;coagulans Isolates
This study employed a comprehensive approach, utilizing comparative genomics, to identify genomic distinctions between S. schleiferi and S. coagulans, aiming to address the challenges in the accurate classification and diagnosis of these organisms and identify unique features. Whole genome sequencing was performed on six clinical isolates, and their genomes were compared to identify variations in gene content and virulence factors. De novo assembly and annotation revealed two samples as S. coagulans and four samples as S. schleiferi. Analysis of the core genomes revealed conserved regions crucial for defining species ident...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Mohamed A. Abouelkhair Stephen A. Kania Tags: Article Source Type: research
Genes, Vol. 15, Pages 286: Data Augmentation Enhances Plant-Genomic-Enabled Predictions
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José Crossa
Genomic selection (GS) is revolutionizing plant breeding. However, its practical implementation is still challenging, since there are many factors that affect its accuracy. For this reason, this research explores data augmentation with the goal of improving its accuracy. Deep neural networks with data augmentation (DA) generate synthetic data from the original training set to increase the training set and to improve the prediction performance of any statistical or machine learning algorithm. There is much empirical evidence of their success in many computer vision applications. Due to this, DA was ex...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Osval A. Montesinos-L ópez Mario Alberto Solis-Camacho Leonardo Crespo-Herrera Carolina Saint Pierre Gloria Isabel Huerta Prado Sofia Ramos-Pulido Khalid Al-Nowibet Roberto Fritsche-Neto Guillermo Gerard Abelardo Montesinos-L ópez Jos é Crossa Tags: Article Source Type: research
Genes, Vol. 15, Pages 287: Prevalence of Endocrinopathies in a Cohort of Patients with Rett Syndrome: A two-Center Observational Study
In conclusion, our data highlighted that endocrinopathies are not rare in RTT, especially in patients with MeCP2 deletions. Therefore, in the context of a multidisciplinary approach, endocrinological evaluation should be recommended for RTT patients. (Source: Genes)
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Giorgia Pepe Roberto Coco Domenico Corica Gabriella Di Di Rosa Filip Bossowski Magdalena Skorupska Tommaso Aversa Stefano Stagi Malgorzata Wasniewska Tags: Article Source Type: research
Genes, Vol. 15, Pages 285: Full-Length Transcriptome Sequencing and RNA-Seq Analysis Offer Insights into Terpenoid Biosynthesis in Blumea balsamifera (L.) DC.
Yuxin Pang
Blumea balsamifera (L.) DC., an important economic and medicinal herb, has a long history of being used as a traditional Chinese medicine. Its leaves have always been used as a raw material for the extraction of essential oils, comprising large amounts of terpenoids, which have good therapeutic effects on many diseases, such as eczema, bacterial infection, and hypertension. However, the genetic basis of terpenoid biosynthesis in this plant is virtually unknown on account of the lack of genomic data. Here, a combination of next-generation sequencing (NGS) and full-length transcriptome sequencing was applie...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Zhigang Ju Lin Liang Yaqiang Zheng Hongxi Shi Wenxuan Zhao Wei Sun Yuxin Pang Tags: Article Source Type: research
Genes, Vol. 15, Pages 288: Erythrokeratodermia Variabilis-like Phenotype in Patients Carrying ABCA12 Mutations
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Judith Fischer
Erythrokeratodermia variabilis (EKV) is a rare genodermatosis characterized by well-demarcated erythematous patches and hyperkeratotic plaques. EKV is most often transmitted in an autosomal dominant manner. Until recently, only mutations in connexins such as GJB3 (connexin 31), GJB4 (connexin 30.3), and occasionally GJA1 (connexin 43) were known to cause EKV. In recent years, mutations in other genes have been described as rare causes of EKV, including the genes KDSR, KRT83, and TRPM4. Features of the EKV phenotype can also appear with other genodermatoses: for example, in Netherton syndrome, which h...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Alrun Hotz Regina F ölster-Holst Vinzenz Oji Emmanuelle Bourrat Jorge Frank Slaheddine Marrakchi Mariem Ennouri Lotta Wankner Katalin Komlosi Svenja Alter Judith Fischer Tags: Brief Report Source Type: research
Genes, Vol. 15, Pages 281: SP1 Gene Methylation in Head and Neck Squamous Cell Cancer in HPV-Negative Patients
mur Fatkhudinov
There is still much to learn about the epigenetic mechanisms controlling gene expression during carcinogenesis. When researching aberrant DNA methylation, active proliferative tumor cells from head and neck squamous cell cancer (HNSCC) can be used as a model. The aim of the study was to investigate the methylation status of CDKN1, CDKN2A, MYC, Smad3, SP1, and UBC genes in tumor tissue (control-normal tissue) in 50 patients (37 men and 13 women) with HPV-negative HNSCC. Methods: Bisulfite conversion methods and methyl-sensitive analysis of high-resolution melting curves were used to quantify the methylat...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Enar Jumaniyazova Anna Aghajanyan Sergey Kurevlev Leyla Tskhovrebova Andrey Makarov Konstantin Gordon Anastasiya Lokhonina Timur Fatkhudinov Tags: Brief Report Source Type: research
Genes, Vol. 15, Pages 282: Effects of Paraquat, Dextran Sulfate Sodium, and Irradiation on Behavioral and Cognitive Performance and the Gut Microbiome in A53T and A53T-L444P Mice
Thomas Sharpton
Jacob Raber
Heterozygous carriers of the glucocerebrosidase 1 (GBA) L444P Gaucher mutation have an increased risk of developing Parkinson’s disease (PD). The GBA mutations result in elevated alpha synuclein (aSyn) levels. Heterozygous mice carrying one allele with the L444P mutation knocked-into the mouse gene show increased aSyn levels and are more sensitive to motor deficits following exposure to the neurotoxin (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) MPTP than wild-type mice. Paraquat (PQ), a herbicide, increases PD risk in most studies. Its effects on the brain involve alterat...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Ariel Chaklai Abigail O ’Neil Shrey Goel Nick Margolies Destine Krenik Ruby Perez Kat Kessler Elizabeth Staltontall Hong Ki (Eric) Yoon Montzerrat Pantoja Keaton Stagaman Kristin Kasschau Vivek Unni Robert Duvoisin Thomas Sharpton Jacob Raber Tags: Article Source Type: research
Genes, Vol. 15, Pages 277: Identification and Validation of Reference Genes for qRT-PCR Analysis of Petal-Color-Related Genes in Rosa praelucens
Lulin Ma
The flower’s color is regarded as one of the most outstanding features of the rose. Rosa praelucens Byhouwer, an endemic and critically endangered decaploid wild rose species, is abundant in phenotypic diversity, especially in flower color variation, from white to different degrees of pink. The mechanism underlying this variation, e.g., the level of petal-color-related genes, is worth probing. Seven candidate reference genes for qRT-PCR analysis, including tubulin α chain (TUBA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H2B (Histone2A), eukaryotic translation elong...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Hongying Jian Huichun Wang Xianqin Qiu Huijun Yan Lulin Ma Tags: Article Source Type: research
