Genes, Vol. 15, Pages 289: Heat-Resistant Inbred Lines Coordinate the Heat Response Gene Expression Remarkably in Maize (Zea mays L.)
ua Chen
High temperatures are increasingly becoming a prominent environmental factor accelerating the adverse influence on the growth and development of maize (Zea mays L.). Therefore, it is critical to identify the key genes and pathways related to heat stress (HS) tolerance in maize. Great challenges have been faced in dissecting genetic mechanisms and uncovering master genes for HS tolerance. Here, Z58D showed more thermotolerance than AF171 at the seedling stage with a lower wilted leaf rate and H2O2 accumulation under HS conditions. Transcriptomic analysis identified 3006 differentially expressed genes (DEGs) in A...
Source: Genes - February 25, 2024 Category: Genetics & Stem Cells Authors: Ming Xue Xiaoyue Han Luyao Zhang Saihua Chen Tags: Article Source Type: research
Genes, Vol. 15, Pages 283: Transcriptomic Profiling at the Maternal-to-Zygotic Transition in Leech, Helobdella austinensis
In this study, we focused on a narrow developmental time window of ~6–8 h, comprising stages just prior to and immediately following zygote deposition. Employing RNA-Seq methodology, we identified differentially expressed transcripts at this fundamental ontogenic boundary, known as the maternal-to-zygotic transition (MZT). Gene expression changes were characterized by the massive degradation of maternal RNAs (~45%) coupled with the rapid transcription of ~5000 zygotic genes (~20% of the genome) in the first mitotic cell cycle. The latter transcripts encoded a mixture of cell maintenance and regulatory protein...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Samuel Hsaio Naim Saglam David Morrow Daniel H. Shain Tags: Article Source Type: research
Genes, Vol. 15, Pages 284: Whole Genome Sequencing and Comparative Genomics of Six Staphylococcus schleiferi and Staphylococcus & nbsp;coagulans Isolates
This study employed a comprehensive approach, utilizing comparative genomics, to identify genomic distinctions between S. schleiferi and S. coagulans, aiming to address the challenges in the accurate classification and diagnosis of these organisms and identify unique features. Whole genome sequencing was performed on six clinical isolates, and their genomes were compared to identify variations in gene content and virulence factors. De novo assembly and annotation revealed two samples as S. coagulans and four samples as S. schleiferi. Analysis of the core genomes revealed conserved regions crucial for defining species ident...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Mohamed A. Abouelkhair Stephen A. Kania Tags: Article Source Type: research
Genes, Vol. 15, Pages 286: Data Augmentation Enhances Plant-Genomic-Enabled Predictions
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José Crossa
Genomic selection (GS) is revolutionizing plant breeding. However, its practical implementation is still challenging, since there are many factors that affect its accuracy. For this reason, this research explores data augmentation with the goal of improving its accuracy. Deep neural networks with data augmentation (DA) generate synthetic data from the original training set to increase the training set and to improve the prediction performance of any statistical or machine learning algorithm. There is much empirical evidence of their success in many computer vision applications. Due to this, DA was ex...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Osval A. Montesinos-L ópez Mario Alberto Solis-Camacho Leonardo Crespo-Herrera Carolina Saint Pierre Gloria Isabel Huerta Prado Sofia Ramos-Pulido Khalid Al-Nowibet Roberto Fritsche-Neto Guillermo Gerard Abelardo Montesinos-L ópez Jos é Crossa Tags: Article Source Type: research
Genes, Vol. 15, Pages 287: Prevalence of Endocrinopathies in a Cohort of Patients with Rett Syndrome: A two-Center Observational Study
In conclusion, our data highlighted that endocrinopathies are not rare in RTT, especially in patients with MeCP2 deletions. Therefore, in the context of a multidisciplinary approach, endocrinological evaluation should be recommended for RTT patients. (Source: Genes)
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Giorgia Pepe Roberto Coco Domenico Corica Gabriella Di Di Rosa Filip Bossowski Magdalena Skorupska Tommaso Aversa Stefano Stagi Malgorzata Wasniewska Tags: Article Source Type: research
Genes, Vol. 15, Pages 285: Full-Length Transcriptome Sequencing and RNA-Seq Analysis Offer Insights into Terpenoid Biosynthesis in Blumea balsamifera (L.) DC.
Yuxin Pang
Blumea balsamifera (L.) DC., an important economic and medicinal herb, has a long history of being used as a traditional Chinese medicine. Its leaves have always been used as a raw material for the extraction of essential oils, comprising large amounts of terpenoids, which have good therapeutic effects on many diseases, such as eczema, bacterial infection, and hypertension. However, the genetic basis of terpenoid biosynthesis in this plant is virtually unknown on account of the lack of genomic data. Here, a combination of next-generation sequencing (NGS) and full-length transcriptome sequencing was applie...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Zhigang Ju Lin Liang Yaqiang Zheng Hongxi Shi Wenxuan Zhao Wei Sun Yuxin Pang Tags: Article Source Type: research
Genes, Vol. 15, Pages 288: Erythrokeratodermia Variabilis-like Phenotype in Patients Carrying ABCA12 Mutations
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Judith Fischer
Erythrokeratodermia variabilis (EKV) is a rare genodermatosis characterized by well-demarcated erythematous patches and hyperkeratotic plaques. EKV is most often transmitted in an autosomal dominant manner. Until recently, only mutations in connexins such as GJB3 (connexin 31), GJB4 (connexin 30.3), and occasionally GJA1 (connexin 43) were known to cause EKV. In recent years, mutations in other genes have been described as rare causes of EKV, including the genes KDSR, KRT83, and TRPM4. Features of the EKV phenotype can also appear with other genodermatoses: for example, in Netherton syndrome, which h...
Source: Genes - February 24, 2024 Category: Genetics & Stem Cells Authors: Alrun Hotz Regina F ölster-Holst Vinzenz Oji Emmanuelle Bourrat Jorge Frank Slaheddine Marrakchi Mariem Ennouri Lotta Wankner Katalin Komlosi Svenja Alter Judith Fischer Tags: Brief Report Source Type: research
Genes, Vol. 15, Pages 281: SP1 Gene Methylation in Head and Neck Squamous Cell Cancer in HPV-Negative Patients
mur Fatkhudinov
There is still much to learn about the epigenetic mechanisms controlling gene expression during carcinogenesis. When researching aberrant DNA methylation, active proliferative tumor cells from head and neck squamous cell cancer (HNSCC) can be used as a model. The aim of the study was to investigate the methylation status of CDKN1, CDKN2A, MYC, Smad3, SP1, and UBC genes in tumor tissue (control-normal tissue) in 50 patients (37 men and 13 women) with HPV-negative HNSCC. Methods: Bisulfite conversion methods and methyl-sensitive analysis of high-resolution melting curves were used to quantify the methylat...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Enar Jumaniyazova Anna Aghajanyan Sergey Kurevlev Leyla Tskhovrebova Andrey Makarov Konstantin Gordon Anastasiya Lokhonina Timur Fatkhudinov Tags: Brief Report Source Type: research
Genes, Vol. 15, Pages 282: Effects of Paraquat, Dextran Sulfate Sodium, and Irradiation on Behavioral and Cognitive Performance and the Gut Microbiome in A53T and A53T-L444P Mice
Thomas Sharpton
Jacob Raber
Heterozygous carriers of the glucocerebrosidase 1 (GBA) L444P Gaucher mutation have an increased risk of developing Parkinson’s disease (PD). The GBA mutations result in elevated alpha synuclein (aSyn) levels. Heterozygous mice carrying one allele with the L444P mutation knocked-into the mouse gene show increased aSyn levels and are more sensitive to motor deficits following exposure to the neurotoxin (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) MPTP than wild-type mice. Paraquat (PQ), a herbicide, increases PD risk in most studies. Its effects on the brain involve alterat...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Ariel Chaklai Abigail O ’Neil Shrey Goel Nick Margolies Destine Krenik Ruby Perez Kat Kessler Elizabeth Staltontall Hong Ki (Eric) Yoon Montzerrat Pantoja Keaton Stagaman Kristin Kasschau Vivek Unni Robert Duvoisin Thomas Sharpton Jacob Raber Tags: Article Source Type: research
Genes, Vol. 15, Pages 277: Identification and Validation of Reference Genes for qRT-PCR Analysis of Petal-Color-Related Genes in Rosa praelucens
Lulin Ma
The flower’s color is regarded as one of the most outstanding features of the rose. Rosa praelucens Byhouwer, an endemic and critically endangered decaploid wild rose species, is abundant in phenotypic diversity, especially in flower color variation, from white to different degrees of pink. The mechanism underlying this variation, e.g., the level of petal-color-related genes, is worth probing. Seven candidate reference genes for qRT-PCR analysis, including tubulin α chain (TUBA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H2B (Histone2A), eukaryotic translation elong...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Hongying Jian Huichun Wang Xianqin Qiu Huijun Yan Lulin Ma Tags: Article Source Type: research
Genes, Vol. 15, Pages 278: Human Leukocyte Antigen Markers for Distinguishing Pustular Psoriasis and Adult-Onset Immunodeficiency with Pustular Reaction
Napatra Tovanabutra
Siri Chiewchanvit
Piranit Kantaputra
Worrachet Intachai
Sivamoke Dissook
Mati Chuamanochan
Pustular skin diseases, with pustular psoriasis (PP) being the prototype, are immune-mediated diseases characterized by the presence of multiple pustules, resulting from neutrophil accumulation in the layer of epidermis. Sterile skin pustular eruption, like PP, is also observed in 20–30% of patients with adult-onset immunodeficiency syndrome (AOID) and anti-interferon γ autoantibodies (IFN-γ), leading to challenges in classification and diagnosis. While the...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Apiwat Sangphukieo Patcharawadee Thongkumkoon Pitiporn Noisagul Luca Lo Piccolo Timothy E. O ’Brien Suteeraporn Chaowattanapanit Charoen Choonhakarn Warayuwadee Amornpinyo Romanee Chaiwarith Salin Kiratikanon Rujira Rujiwetpongstorn Napatra Tovanabutra Tags: Article Source Type: research
Genes, Vol. 15, Pages 279: Evaluation of Storage Conditions and the Effect on DNA from Forensic Evidence Objects Retrieved from Lake Water
This study illustrates that retrieval from water as soon as possible and immediate storage through air-drying or freezing before DNA analysis is beneficial for the outcome of DNA profiling in crime scene investigations. (Source: Genes)
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Muhammad Shahzad Hanne De Maeyer Ghassan Ali Salih Martina Nilsson Anastasia Haratourian Muhammad Shafique Ahmad Ali Shahid Marie Allen Tags: Article Source Type: research
Genes, Vol. 15, Pages 280: POPDC1 Variants Cause Atrioventricular Node Dysfunction and Arrhythmogenic Changes in Cardiac Electrophysiology and Intracellular Calcium Handling in Zebrafish
. Alexander Quinn
Popeye domain-containing (POPDC) proteins selectively bind cAMP and mediate cellular responses to sympathetic nervous system (SNS) stimulation. The first discovered human genetic variant (POPDC1S201F) is associated with atrioventricular (AV) block, which is exacerbated by increased SNS activity. Zebrafish carrying the homologous mutation (popdc1S191F) display a similar phenotype to humans. To investigate the impact of POPDC1 dysfunction on cardiac electrophysiology and intracellular calcium handling, homozygous popdc1S191F and popdc1 knock-out (popdc1KO) zebrafish larvae and adult isolated popdc1S191F...
Source: Genes - February 23, 2024 Category: Genetics & Stem Cells Authors: Matthew R. Stoyek Sarah E. Doane Shannon E. Dallaire Zachary D. Long Jessica M. Ramia Donovan L. Cassidy-Nolan Kar-Lai Poon Thomas Brand T. Alexander Quinn Tags: Article Source Type: research
Genes, Vol. 15, Pages 273: Effects of Soil Rhizobia Abundance on Interactions between a Vector, Pathogen, and Legume Plant Host
. Crowder
Soil rhizobia promote nitrogen fixation in legume hosts, maximizing their tolerance to different biotic stressors, plant biomass, crop growth, and yield. While the presence of soil rhizobia is considered beneficial for plants, few studies have assessed whether variation in rhizobia abundance affects the tolerance of legumes to stressors. To address this, we assessed the effects of variable soil rhizobia inoculum concentrations on interactions between a legume host (Pisum sativum), a vector insect (Acyrthosiphon pisum), and a virus (Pea enation mosaic virus, PEMV). We showed that increased rhizobia abundance r...
Source: Genes - February 22, 2024 Category: Genetics & Stem Cells Authors: Pooja Malhotra Saumik Basu Benjamin W. Lee Liesl Oeller David W. Crowder Tags: Article Source Type: research
Genes, Vol. 15, Pages 274: Identification of Clubroot (Plasmodiophora brassicae) Resistance Loci in Chinese Cabbage (Brassica rapa ssp. pekinensis) with Recessive Character
In this study, Bap246 was tested by being crossed with different highly susceptible B. rapa materials and showed recessive resistance to clubroot. An F2 population derived from Bap246 × Bac1344 was used to locate the resistance Quantitative Trait Loci (QTL) by Bulk Segregant Analysis Sequencing (BSA-Seq) and QTL mapping methods. Two QTL on chromosomes A01 (4.67–6.06 Mb) and A08 (10.42–11.43 Mb) were found and named Cr4Ba1.1 and Cr4Ba8.1, respectively. Fifteen and eleven SNP/InDel markers were used to narrow the target regions in the larger F2 population to 4.67–5.17 M...
Source: Genes - February 22, 2024 Category: Genetics & Stem Cells Authors: Hui Zhang Xitong Liu Jinyan Zhou Stephen E. Strelkov Rudolph Fredua-Agyeman Shifan Zhang Fei Li Guoliang Li Jian Wu Rifei Sun Sheau-Fang Hwang Shujiang Zhang Tags: Article Source Type: research
