Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production
This study focused on the functional investigation of the pbpcz gene in P. brevicompactum, a regulator belonging to the Zn(II)2Cys6 family. RNAi was used to silence the pbpcz gene, providing a valuable tool for genetic studies in P. brevicompactum. After seven days, we observed differences in the number of spores between different phenotypes strains of pbpcz gene. Compared to the wild-type strain (WT), the spore yield of the pbpcz gene silencing mutant (M2) was only 51.4 %, while that of the pbpcz gene overexpressed mutant (SE4) was increased by 50 %. Expression levels of the three genes (brlA, abaA, and wetA) comprising c...
Source: Fungal Genetics and Biology - November 3, 2023 Category: Biology Authors: TingTing Hu Jingjing Wang Mianhui Chen Lin Lin Wei Wei Dongzhi Wei Source Type: research
Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production
This study focused on the functional investigation of the pbpcz gene in P. brevicompactum, a regulator belonging to the Zn(II)2Cys6 family. RNAi was used to silence the pbpcz gene, providing a valuable tool for genetic studies in P. brevicompactum. After seven days, we observed differences in the number of spores between different phenotypes strains of pbpcz gene. Compared to the wild-type strain (WT), the spore yield of the pbpcz gene silencing mutant (M2) was only 51.4 %, while that of the pbpcz gene overexpressed mutant (SE4) was increased by 50 %. Expression levels of the three genes (brlA, abaA, and wetA) comprising c...
Source: Fungal Genetics and Biology - November 3, 2023 Category: Biology Authors: TingTing Hu Jingjing Wang Mianhui Chen Lin Lin Wei Wei Dongzhi Wei Source Type: research
Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production
This study focused on the functional investigation of the pbpcz gene in P. brevicompactum, a regulator belonging to the Zn(II)2Cys6 family. RNAi was used to silence the pbpcz gene, providing a valuable tool for genetic studies in P. brevicompactum. After seven days, we observed differences in the number of spores between different phenotypes strains of pbpcz gene. Compared to the wild-type strain (WT), the spore yield of the pbpcz gene silencing mutant (M2) was only 51.4 %, while that of the pbpcz gene overexpressed mutant (SE4) was increased by 50 %. Expression levels of the three genes (brlA, abaA, and wetA) comprising c...
Source: Fungal Genetics and Biology - November 3, 2023 Category: Biology Authors: TingTing Hu Jingjing Wang Mianhui Chen Lin Lin Wei Wei Dongzhi Wei Source Type: research
Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production
This study focused on the functional investigation of the pbpcz gene in P. brevicompactum, a regulator belonging to the Zn(II)2Cys6 family. RNAi was used to silence the pbpcz gene, providing a valuable tool for genetic studies in P. brevicompactum. After seven days, we observed differences in the number of spores between different phenotypes strains of pbpcz gene. Compared to the wild-type strain (WT), the spore yield of the pbpcz gene silencing mutant (M2) was only 51.4 %, while that of the pbpcz gene overexpressed mutant (SE4) was increased by 50 %. Expression levels of the three genes (brlA, abaA, and wetA) comprising c...
Source: Fungal Genetics and Biology - November 3, 2023 Category: Biology Authors: TingTing Hu Jingjing Wang Mianhui Chen Lin Lin Wei Wei Dongzhi Wei Source Type: research
Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production
This study focused on the functional investigation of the pbpcz gene in P. brevicompactum, a regulator belonging to the Zn(II)2Cys6 family. RNAi was used to silence the pbpcz gene, providing a valuable tool for genetic studies in P. brevicompactum. After seven days, we observed differences in the number of spores between different phenotypes strains of pbpcz gene. Compared to the wild-type strain (WT), the spore yield of the pbpcz gene silencing mutant (M2) was only 51.4 %, while that of the pbpcz gene overexpressed mutant (SE4) was increased by 50 %. Expression levels of the three genes (brlA, abaA, and wetA) comprising c...
Source: Fungal Genetics and Biology - November 3, 2023 Category: Biology Authors: TingTing Hu Jingjing Wang Mianhui Chen Lin Lin Wei Wei Dongzhi Wei Source Type: research
Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production
This study focused on the functional investigation of the pbpcz gene in P. brevicompactum, a regulator belonging to the Zn(II)2Cys6 family. RNAi was used to silence the pbpcz gene, providing a valuable tool for genetic studies in P. brevicompactum. After seven days, we observed differences in the number of spores between different phenotypes strains of pbpcz gene. Compared to the wild-type strain (WT), the spore yield of the pbpcz gene silencing mutant (M2) was only 51.4 %, while that of the pbpcz gene overexpressed mutant (SE4) was increased by 50 %. Expression levels of the three genes (brlA, abaA, and wetA) comprising c...
Source: Fungal Genetics and Biology - November 3, 2023 Category: Biology Authors: TingTing Hu Jingjing Wang Mianhui Chen Lin Lin Wei Wei Dongzhi Wei Source Type: research
Construction of RNA silencing system of Penicillium brevicompactum and genetic manipulation of the regulator pbpcz in mycophenolic acid production
This study focused on the functional investigation of the pbpcz gene in P. brevicompactum, a regulator belonging to the Zn(II)2Cys6 family. RNAi was used to silence the pbpcz gene, providing a valuable tool for genetic studies in P. brevicompactum. After seven days, we observed differences in the number of spores between different phenotypes strains of pbpcz gene. Compared to the wild-type strain (WT), the spore yield of the pbpcz gene silencing mutant (M2) was only 51.4 %, while that of the pbpcz gene overexpressed mutant (SE4) was increased by 50 %. Expression levels of the three genes (brlA, abaA, and wetA) comprising c...
Source: Fungal Genetics and Biology - November 3, 2023 Category: Biology Authors: TingTing Hu Jingjing Wang Mianhui Chen Lin Lin Wei Wei Dongzhi Wei Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
A member of the OSCA/TMEM63 family of mechanosensitive calcium channels participates in cell wall integrity maintenance in Aspergillus nidulans
Fungal Genet Biol. 2023 Oct 5;169:103842. doi: 10.1016/j.fgb.2023.103842. Online ahead of print.ABSTRACTThe calF7 mutation in Aspergillus nidulans causes hypersensitivity to the cell wall compromising agents Calcofluor White (CFW) and Congo Red. In this research we demonstrate that the calF7 mutation resides in gene AN2880, encoding a predicted member of the OSCA/TMEM63 family of transmembrane glycoproteins. Those members of the family whose physiological functions have been investigated have been shown to act as mechanosensitive calcium transport channels. Deletion of AN2880 replicates the CFW hypersensitivity phenotype. ...
Source: Fungal Genetics and Biology - October 7, 2023 Category: Biology Authors: Terry W Hill Stanley Vance Jennifer F Loome Benard J Haugen Darlene M Loprete Shana V Stoddard Loretta Jackson-Hayes Source Type: research
