Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: Deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 27;170:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: Deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 27;170:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: Deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 27;170:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: Deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 27;170:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: Deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 27;170:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 26:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use in ...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 26:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use in ...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Creating large chromosomal segment deletions in Aspergillus flavus by a dual CRISPR/Cas9 system: deletion of gene clusters for production of aflatoxin, cyclopiazonic acid, and ustiloxin B
Fungal Genet Biol. 2023 Dec 26:103863. doi: 10.1016/j.fgb.2023.103863. Online ahead of print.ABSTRACTAspergillus flavus produces hepatocarcinogenic aflatoxin that adversely impacts human and animal health and international trade. A promising means to manage preharvest aflatoxin contamination of crops is biological control, which employs non-aflatoxigenic A. flavus isolates possessing defective aflatoxin gene clusters to outcompete field toxigenic populations. However, these isolates often produce other toxic metabolites. The CRISPR/Cas9 technology has greatly advanced genome editing and gene functional studies. Its use in ...
Source: Fungal Genetics and Biology - December 28, 2023 Category: Biology Authors: Perng-Kuang Chang Source Type: research
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to...
Source: Fungal Genetics and Biology - December 21, 2023 Category: Biology Authors: M Cogliati P E Chidebelu M Hitchcock M Chen V Rickerts S Ackermann M Desnos Ollivier J In ácio U Nawrot M Florek K J Kwon-Chung D-H Yang C Firacative C A Puime P Escandon S Bertout F Roger J Xu Source Type: research
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to...
Source: Fungal Genetics and Biology - December 21, 2023 Category: Biology Authors: M Cogliati P E Chidebelu M Hitchcock M Chen V Rickerts S Ackermann M Desnos Ollivier J In ácio U Nawrot M Florek K J Kwon-Chung D-H Yang C Firacative C A Puime P Escandon S Bertout F Roger J Xu Source Type: research
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to...
Source: Fungal Genetics and Biology - December 21, 2023 Category: Biology Authors: M Cogliati P E Chidebelu M Hitchcock M Chen V Rickerts S Ackermann M Desnos Ollivier J In ácio U Nawrot M Florek K J Kwon-Chung D-H Yang C Firacative C A Puime P Escandon S Bertout F Roger J Xu Source Type: research
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to...
Source: Fungal Genetics and Biology - December 21, 2023 Category: Biology Authors: M Cogliati P E Chidebelu M Hitchcock M Chen V Rickerts S Ackermann M Desnos Ollivier J In ácio U Nawrot M Florek K J Kwon-Chung D-H Yang C Firacative C A Puime P Escandon S Bertout F Roger J Xu Source Type: research
Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids
In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to...
Source: Fungal Genetics and Biology - December 21, 2023 Category: Biology Authors: M Cogliati P E Chidebelu M Hitchcock M Chen V Rickerts S Ackermann M Desnos Ollivier J In ácio U Nawrot M Florek K J Kwon-Chung D-H Yang C Firacative C A Puime P Escandon S Bertout F Roger J Xu Source Type: research
Multi-locus sequence typing and phylogenetics of cryptococcus neoformans ad hybrids
In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to...
Source: Fungal Genetics and Biology - December 21, 2023 Category: Biology Authors: M Cogliati P E Chidebelu M Hitchcock M Chen V Rickerts S Ackermann M Desnos Ollivier J In ácio U Nawrot M Flore K J Kwon Chung D-H Yang C Firacative C A Puime P Escandon S Bertout F Roger J Xu Source Type: research
Pathogenicity chromosome of Fusarium oxysporum f. sp. cepae
Fungal Genet Biol. 2023 Dec 17;170:103860. doi: 10.1016/j.fgb.2023.103860. Online ahead of print.ABSTRACTFusarium oxysporum f. sp. cepae (Foc) is the causative agent of Fusarium basal rot disease in onions, which leads to catastrophic global crop production losses. Therefore, the interaction of Foc with its host has been actively investigated, and the pathogen-specific (PS) regions of the British strain Foc_FUS2 have been identified. However, it has not been experimentally determined whether the identified PS region plays a role in pathogenicity. To identify the pathogenicity chromosome in the Japanese strain Foc_TA, we in...
Source: Fungal Genetics and Biology - December 19, 2023 Category: Biology Authors: Kosei Sakane Mitsunori Akiyama Sudisha Jogaiah Shin-Ichi Ito Kazunori Sasaki Source Type: research
