Genome editing of different strains of Aureobasidium melanogenum using efficient Cre/loxp site-specific recombination systems
In this study, an efficient Cre/loxp site-specific recombination system (pAMGDloxp-1, pAMEXlox-1 and pAMCRE1) was constructed. It was found that they could be successfully used to sequentially delete and express many genes in different strains of A. melanogenum. After each round of gene disruption and expression, over 0.5 positive cells per 1000 competent cells and over 49.8 positive transformants per 1.0 μg DNA were achieved. After each round of the antibiotics gene excision by using the Cre-loxp site-specific recombination, over 95.4% of the antibiotics-resistant cells became sensitive to both hygromycin B and nourseoth...
Source: Fungal Biology - June 13, 2019 Category: Biology Source Type: research

Polysaccharide diversity in VNI isolates of Cryptococcus neoformans from Roraima, Northern Brazil
Publication date: Available online 12 June 2019Source: Fungal BiologyAuthor(s): Halan D. Dal Pupo, Bianca A.G. Sena, Flavia C.G. Reis, Lumena Machado, Silvana T. Fortes, João N. de Almeida Junior, Rodrigo M.C. Godinho, Marcio L. RodriguesAbstractSpecies of the Cryptococcus genus comprise environmental, encapsulated fungal pathogens that cause lethal meningitis in immunosuppressed individuals. In humans, fungal uptake of hypocapsular Cryptococcus by macrophages was associated with high fungal burden in the cerebrospinal fluid and long-term patient survival. On the basis of the key role of the cryptococcal capsule in diseas...
Source: Fungal Biology - June 12, 2019 Category: Biology Source Type: research

Subunits of the vacuolar H+-ATPase complex, Vma4 and Vma10, are essential for virulence and represent potential drug targets in Candida albicans
Publication date: Available online 12 June 2019Source: Fungal BiologyAuthor(s): Se Woong Kim, Young Kwang Park, Yoo Jin Joo, Yu Jin Chun, Ju Yeon Hwang, Je-Hyun Baek, Joon KimAbstractHyphal morphogenesis of Candida albicans is important for its pathogenesis. Here, we showed that the filamentous growth of C. albicans requires vacuolar H+-ATPase function. Results showed that levels of Vma4 and Vma10 increased in cells undergoing hyphal growth compared to those undergoing yeast growth. Deleting VMA4 or VMA10 abolished vacuolar functions and hyphal morphogenesis. These deletion mutants were also characterized as avirulent in a...
Source: Fungal Biology - June 12, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: July 2019Source: Fungal Biology, Volume 123, Issue 7Author(s): (Source: Fungal Biology)
Source: Fungal Biology - June 12, 2019 Category: Biology Source Type: research

The first survey of Cystobasidiomycete yeasts in the lichen genus Cladonia; with the description of Lichenozyma pisutiana gen. nov., sp. nov
We report the first known associations between cystobasidiomycete yeasts and Cladonia (both corticate and ecorticate), and find that the association is geographically widespread in various habitats. Our results also suggest that a great diversity of lichenassociated yeasts remains to be discovered. (Source: Fungal Biology)
Source: Fungal Biology - June 7, 2019 Category: Biology Source Type: research

The concentration and prevalence of ochratoxin A in coffee and coffee-based products: a global systematic review, meta-analysis and meta-regression
Publication date: Available online 4 June 2019Source: Fungal BiologyAuthor(s): Amin Mousavi Khaneghah, Yadolah Fakhri, Leili Abdi, Carolina Fernanda Sengling Cebin Coppa, Larissa Tuanny Franco, Carlos Augusto Fernandes de OliveiraAbstractThe contamination of food products with mycotoxin always is a matter of concern for both academic and industry. In this regard, the current investigation was aimed to estimate the prevalence and concentration of ochratoxin A (OTA) in different types of coffee and coffee-based products with the aid of a systematic review and meta-analysis. Therefore, the recommended databases including PubM...
Source: Fungal Biology - June 6, 2019 Category: Biology Source Type: research

Comparison of aquatic hyphomycetes communities between lotic and lentic environments in the Atlantic Rain Forest of Pernambuco, Northeast Brazil
Publication date: Available online 31 May 2019Source: Fungal BiologyAuthor(s): Gabriela Virgínia Ramos da Silva, Rafael F. Castañeda-Ruiz, Elaine MalossoAbstractRiparian forests are known for their importance to aquatic ecosystems. In the regions of riparian forests, large quantities of organic matter are produced and recycled by the microbial community that includes microscopic fungi. The aim of this study was to unveil and compare the diversity of aquatic hyphomycetes associated to submerged leaf litter of tropical lotic and lentic environments in the Atlantic Forest of Northeast Brazil. Six sampling events were carrie...
Source: Fungal Biology - June 1, 2019 Category: Biology Source Type: research

Transformation of Corynespora cassiicola by Agrobacterium tumefaciens
Publication date: Available online 30 May 2019Source: Fungal BiologyAuthor(s): Junxiang Li, Ni Hong, Bin Peng, Huijie Wu, Qinsheng GuAbstractThe fungus causing target spot disease, Corynespora cassiicola (Berk. & M. A. Curtis) C. T. Wei, poses an increasing threat to watermelon (Citrullus lanatus), muskmelon (Cucumis melo), and cucumber (Cucumis sativus); the most economically important cucurbit crops grown in China. An understanding of the molecular mechanisms underlying the pathogenicity of C. cassiicola is essential for the development of new strategies to control this disease-causing fungus. Agrobacterium tumefaciens-m...
Source: Fungal Biology - May 30, 2019 Category: Biology Source Type: research

Environmental characteristics and taxonomy of microscopic fungi isolated from washing machines
This study is focused on the diversity of fungal species colonising washing machines, and investigates the possible relationship between the habits of washing machine users and the colonising species. Housekeeping conditions and habits of washing machine users were assessed in Hungary with a questionnaire. After sampling, several different fungal species were identified by microscopy and sequence analysis of diagnostic loci. Based on the results, 32% of the sampled washing machines were highly polluted with various species of fungi. Forty six percent of the observed devices were colonised also by opportunistically human pa...
Source: Fungal Biology - May 25, 2019 Category: Biology Source Type: research

The central role of septa in the basidiomycete Schizophyllum commune hyphal morphogenesis
Publication date: Available online 22 May 2019Source: Fungal BiologyAuthor(s): Marjatta RaudaskoskiAbstractThe purpose of the present research was to observe in the filamentous basidiomycete Schizophyllum commune, the connection between the nuclear division and polymerization of the contractile actin ring with subsequent formation of septa in living hyphae. The filamentous actin was visualized using Lifeact-mCherry and the nuclei with EGFP tagged histone 2B (H2B). Time-lapse fluorescence microscopy confirmed that both in monokaryotic (haploid) and dikaryotic hyphae, the first signs of the contractile actin ring occur at th...
Source: Fungal Biology - May 24, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: June 2019Source: Fungal Biology, Volume 123, Issue 6Author(s): (Source: Fungal Biology)
Source: Fungal Biology - May 22, 2019 Category: Biology Source Type: research

The pH sensing receptor AopalH plays important roles in the nematophagous fungus Arthrobotrys oligospora
Publication date: Available online 18 May 2019Source: Fungal BiologyAuthor(s): Juan Li, Runian Wu, Meng Wang, James Borneman, Jinkui Yang, Ke-Qin ZhangAbstractThere is well-conserved PacC/Rim101 signaling among ascomycete fungi to mediate environmental pH sensing. For pathogenic fungi, this pathway not only enables fungi to grow over a wide pH range, but it also determines whether these fungi can successfully colonize and invade the targeted host. Within the pal/PacC pathway, palH is a putative ambient pH sensor with a seven-transmembrane domain. To characterize the function of a palH homolog, AopalH, in the nematophagous ...
Source: Fungal Biology - May 19, 2019 Category: Biology Source Type: research

The α-1,6-mannosyltransferase VdOCH1 plays a major role in microsclerotium formation and virulence in the soil-borne pathogen Verticillium dahliae
In conclusion, VdOCH1, encoded α-1,6-mannosyltransferase, manipulating the biological characteristics, microsclerotia formation and pathogenic ability of V. dahliae in sunflower. (Source: Fungal Biology)
Source: Fungal Biology - May 18, 2019 Category: Biology Source Type: research

Diversity and distribution of hidden cultivable fungi associated with marine animals of Antarctica
Publication date: Available online 14 May 2019Source: Fungal BiologyAuthor(s): Valéria Martins Godinho, Maria Theresa Rafaela de Paula, Débora Amorim Saraiva Silva, Karla Paresque, Aline Paternostro Martins, Pio Colepicolo, Carlos Augusto Rosa, Luiz Henrique RosaAbstractIn the present study, we surveyed the distribution and diversity of fungal assemblages associated with 10 species of marine animals from Antarctica. The collections yielded 83 taxa from 27 distinct genera, which were identified using molecular biology methods. The most abundant taxa were Cladosporium sp. 1, Debaryomyces hansenii, Glaciozyma martinii, Mets...
Source: Fungal Biology - May 15, 2019 Category: Biology Source Type: research

Penicillium digitatum infection mechanisms in citrus: What do we know so far?
Publication date: Available online 13 May 2019Source: Fungal BiologyAuthor(s): Jonas Henrique Costa, Jaqueline Moraes Bazioli, João Guilherme de Moraes Pontes, Taícia Pacheco FillAbstractPenicillium digitatum is the major source of postharvest decay in citrus fruits worldwide. This fungus shows a notably limited host range, being able to infect mainly mature fruit belonging to the Rutaceae family. This highly specific host interaction has attracted the interest of the scientific community. Researchers have investigated the chemical interactions and specialized virulence strategies that facilitate this fungus’s fruit co...
Source: Fungal Biology - May 13, 2019 Category: Biology Source Type: research