Using whole genome sequence to compare variant callers and breed differences of US sheep
As whole genome sequence (WGS) data sets have become abundant and widely available, so has the need for variant detection and scoring. The aim of this study was to compare the accuracy of commonly used variant calling programs, Freebayes and GATK HaplotypeCaller (GATK-HC), and to use U.S. sheep WGS data sets to identify novel breed-associated SNPs. Sequence data from 145 sheep consisting of 14 U.S. breeds were filtered and biallelic single nucleotide polymorphisms (SNPs) were retained for genotyping analyses. Genotypes from both programs were compared to each other and to genotypes from bead arrays. The SNPs from WGS were ...
Source: Frontiers in Genetics - January 4, 2023 Category: Genetics & Stem Cells Source Type: research

Case report: Therapy adherence, MTTP variants, and course of atheroma in two patients with HoFH on low-dose, long-term lomitapide therapy
Conclusion: When accompanied by good adherence to therapy, low-dose lomitapide on top of standard lipid-lowering therapy with decreased frequency of lipid apheresis prevented the progression of atherosclerotic burden. Non-compliance might occur due to patient impulsivity and non-adherence to a low-fat diet. (Source: Frontiers in Genetics)
Source: Frontiers in Genetics - January 4, 2023 Category: Genetics & Stem Cells Source Type: research

An integrative analysis of an lncRNA –mRNA competing endogenous RNA network to identify functional lncRNAs in uterine leiomyomas with RNA sequencing
Conclusion: These results suggested that 3,293 DEmRNAs and 553 DElncRNAs were differentially expressed in UL tissue and normal myometrium tissue, which might be candidate-identified therapeutic and prognostic targets for UL and be considered as offering several possible mechanisms and pathogenesis of UL in the future. (Source: Frontiers in Genetics)
Source: Frontiers in Genetics - January 4, 2023 Category: Genetics & Stem Cells Source Type: research

RNA Pol III promoters —key players in precisely targeted plant genome editing
The clustered regularly interspaced short palindrome repeat (CRISPR)/CRISPR-associated protein Cas) system is a powerful and highly precise gene-editing tool in basic and applied research for crop improvement programs. CRISPR/Cas tool is being extensively used in plants to improve crop yield, quality, and nutritional value and make them tolerant to environmental stresses. CRISPR/Cas system consists of a Cas protein with DNA endonuclease activity and one CRISPR RNA transcript that is processed to form one or several short guide RNAs that direct Cas9 to the target DNA sequence. The expression levels of Cas proteins and gRNAs...
Source: Frontiers in Genetics - January 4, 2023 Category: Genetics & Stem Cells Source Type: research

Development of a novel pyroptosis-related LncRNA signature with multiple significance in acute myeloid leukemia
Conclusion: Through comprehensive analysis of PRlncRNA model, our study may offer a valuable basis for future researches in targeting pyroptosis and tumor microenvironment (TME) and provide new measures for prevention and treatment in AML. (Source: Frontiers in Genetics)
Source: Frontiers in Genetics - January 4, 2023 Category: Genetics & Stem Cells Source Type: research