Molecular and antimicrobial characterization of a group G anti-lipopolysaccharide factor (ALF) from Penaeus monodon
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Liang Zhou, Guoqiang Li, Yang Jiao, Danqiong Huang, Anguo Li, Huirong Chen, Ying Liu, Shuiming Li, Hui Li, Chaogang WangAbstractAnti-lipopolysaccharide factors (ALFs) are important host-defense molecules of crustaceans. They all contain a lipopolysaccharide-binding domain (LBD) and some ALFs exhibit strong antimicrobial activity. In this research, a Group G ALF from Penaeus monodon (ALFPm11) was studied. It is an anionic peptide specifically having a cationic and highly amphipathic LBD, with five positively charged residues separate...
Source: Fish and Shellfish Immunology - September 7, 2019 Category: Biology Source Type: research

Immune-related genes response to stimulation of miR-155 overexpression in CIK (ctenopharyngodon idella kidney) cells and zebrafish
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Yonglin Hua, Jing Zhang, Zhihao Jia, Jian Li, Xianrong Xiong, Yan XiongAbstractMiR-155 regulates the development of germinal-center and the generation of immunoglobulin class-switched plasma cells. However, whether miR-155 is involved in immune response in fish is still unclear. Here, CIK cells transfected with miR-155 overexpressed plasmid inhibited mRNA expression of mIg and Rag2 (P 
Source: Fish and Shellfish Immunology - September 7, 2019 Category: Biology Source Type: research

Dietary daidzein improved intestinal health of juvenile turbot in terms of intestinal mucosal barrier function and intestinal microbiota
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Weihao Ou, Haibin Hu, Pei Yang, Jihong Dai, Qinghui Ai, Wenbing Zhang, Yanjiao Zhang, Kangsen MaiAbstractA 12-week feeding trial was conducted to investigate the effect of dietary daidzein on the intestinal mucosal barrier function and the intestinal microbiota profile of juvenile turbot (Scophthalmus maximus L.). Three isonitrogenous and isolipidic experimental diets were formulated to contain 0 (FM), 40 (D.40) and 400 (D.400) mg kg−1 daidzein, respectively. Fish fed D.400 had significantly lower growth performance than fish ...
Source: Fish and Shellfish Immunology - September 7, 2019 Category: Biology Source Type: research

High level of dietary olive oil decreased growth, increased liver lipid deposition and induced inflammation by activating the p38 MAPK and JNK pathways in large yellow croaker (Larimichthys crocea)
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Xueshan Li, Kun Cui, Wei Fang, Qiang Chen, Dan Xu, Kangsen Mai, Yanjiao Zhang, Qinghui AiAbstractA feeding experiment was conducted to determine the effects of fish oil replaced by olive oil (OO) on growth performance, serum biochemical, antioxidant capacity and inflammatory response in large yellow croaker (Larimichthys crocea). Four iso-nitrogenous and iso-lipidic diets were formulated by replacing fish oil (FO) with 0% (the control group), 33.3%, 66.7% and 100% OO. Fish fed the diet with 100% OO had the lowest growth performance ...
Source: Fish and Shellfish Immunology - September 7, 2019 Category: Biology Source Type: research

L-carnitine regulated Nrf2/Keap1 activation in vitro and in vivo and protected oxidized fish oil-induced inflammation response by inhibiting the NF-κB signaling pathway in Rhynchocypris lagowski Dybowski
In conclusion, l-carnitine regulated Nrf2/Keap1 activation in vitro and in vivo and protected oxidized fish oil-induced inflammation response by inhibiting the NF-κB signaling pathway in Rhynchocypris lagowski Dybowski. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - September 6, 2019 Category: Biology Source Type: research

Molecular cloning, expression and functional analysis of Atg16L1 from orange-spotted grouper (Epinephelus coioides)
In this study, we cloned and characterized an Atg16 homolog from orange-spotted grouper (Epinephelus coioides) (EcAtg16L1). EcAtg16L1 encodes a 656-amino acid polypeptide, which shares 94.22% and 72.65% homology with large yellow croakers (Larimichthys crocea) and humans (Homo sapiens), respectively. EcAtg16L1 contains a conserved Atg16 domain and a WD-repeat-containing domain. Subcellular localization showed that EcAtg16L1 was distributed in the cytoplasm of grouper cells with a dot-like pattern. EcAtg16L1 overexpression promoted Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV) re...
Source: Fish and Shellfish Immunology - September 6, 2019 Category: Biology Source Type: research

RNA-seq analysis of local tissue of Carassius auratus gibelio with pharyngeal myxobolosis: Insights into the pharyngeal mucosal immune response in a fish-parasite dialogue
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Yuanli Zhao, Xiuhua Liu, Hiroshi Sato, Qianqian Zhang, Aihua Li, Jinyong ZhangAbstractThe lack of practical control measures for pharyngeal myxobolosis is becoming an important limiting factor for the sustainable development of the gibel carp (Carassius auratus gibelio) culture industry in China. Myxobolus honghuensis has been identified as the causative agent of this pandemic disease, which exclusively infects the pharynx of gibel carp, a potential important mucosal lymphoid-associated tissue (MLAT). Myxozoa generally initiate inva...
Source: Fish and Shellfish Immunology - September 6, 2019 Category: Biology Source Type: research

Major histocompatibility complex class I (MHC Iα) of Japanese flounder (Paralichthys olivaceus) plays a critical role in defense against intracellular pathogen infection
In this study, we examined the expression profile and biological activity of an MHC Iα homologue, PoMHC Iα, from P. olivaceus. Structural analysis showed that PoMHC Iα possesses conserved structural characteristics of MHC Iα proteins, including MHC_I domain, IGc1 domain, transmembrane region. Expression of PoMHC Iα was upregulated in a time-dependent manner by extracellular and intracellular bacterial pathogens and viral pathogen infection. Different expression patterns were exhibited in response to the infection of different types of microbial pathogens in different immune tissues. Recombinan...
Source: Fish and Shellfish Immunology - September 6, 2019 Category: Biology Source Type: research

Chlorpyrifos induced oxidative stress to promote apoptosis and autophagy through the regulation of miR-19a-AMPK axis in common carp
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Qiaojian Zhang, Shufang Zheng, Shengchen Wang, Wei Wang, Houjuan Xing, Shiwen XuAbstractChlorpyrifos (CPF) has become a mainly pollution in water environment. Micro-RNAs (miRNAs) play an important part in the development of apoptosis and autophagy. However, the potential mechanism of CPF induced kidney toxicity and the roles of miRNAs are still unclear. To explore the underlying mechanism, the kidney of common carp exposed to different concentrations of CPF for 40 days was used as a research object. We found that CPF could damage the...
Source: Fish and Shellfish Immunology - September 5, 2019 Category: Biology Source Type: research

Bioencapsulation efficacy of sulfated galactans in adult Artemia salina for enhancing immunity in shrimp Litopenaeus vannamei
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Tawut Rudtanatip, Boonyakorn Boonsri, Jantana Praiboon, Kanokpan WongprasertAbstractLive food organisms like Artemia have been used for delivery of different substances such as nutrients, probiotics and immune-stimulants to aquatic animals. Previously, we reported that sulfated galactans (SG) from the red seaweed Gracilaria fisheri (G. fisheri) increased immune activity in shrimp. In the present study we further investigated the capacity and efficiency of bioencapsulation of SG in adult Artemia for delivery to tissues and potentiall...
Source: Fish and Shellfish Immunology - September 5, 2019 Category: Biology Source Type: research

Grouper IFIT1 inhibits iridovirus and nodavirus infection by positively regulating interferon response
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Ya Zhang, Yuxin Wang, Zetian Liu, Jiaying Zheng, Youhua Huang, Xiaohong Huang, Qiwei QinAbstractInterferon-induced protein with tetratricopeptide repeats 1 (IFIT1), one of the interferon stimulated genes (ISGs), is strongly induced by type I interferon (IFN), double-stranded RNAs and virus infection. To investigate the actions of fish IFIT1 in response to virus infection, we cloned an IFIT1 homolog from orange spotted grouper (EcIFIT1) and clarified its function in this study. The full-length cDNA of EcIFIT1 is 1839 bp, which is com...
Source: Fish and Shellfish Immunology - September 4, 2019 Category: Biology Source Type: research

Astakine1 forms protein complex in plasma
In this study we found that astakine1 was present as a high molecular weight (HMW) complex in plasma. It is known that calcium concentration are fluctuating in several crustaceans especially during the molting process. This HMW-complex was formed under low calcium concentrations in plasma and could be partially reversed provided calcium was added. The biological function of the naïve astakine1 and that in the HMW complex was about the same, but if the protein is to be isolated or studied for its function it is important to know about this property of astakine1 which may previously have hampered isolation and functiona...
Source: Fish and Shellfish Immunology - September 3, 2019 Category: Biology Source Type: research

A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Jianning Zhang, Yifei Zhang, Linbao Zhang, Qianyu Wei, Xiaoli Liu, Dinglong YangAbstractIn the present study, a sialic acid-binding lectin was cloned and characterized from Manila clam Ruditapes philippinarum (designed as RpSabl). The open reading frame of RpSabl encoded a polypeptide of 162 amino acids with a calculated molecular mass of 17.7 kDa. Analysis of the conserved domain suggested that RpSabl was a new member of the sialic acid-binding lectins family. In non-stimulated clams, RpSabl transcripts were constitutively expres...
Source: Fish and Shellfish Immunology - September 3, 2019 Category: Biology Source Type: research

CgSOCS6 negatively regulates the expression of CgIL17s and CgDefh1 in the pacific oyster Crassostrea gigas
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Zhaojun Wu, Jiejie Sun, Liyan Wang, Yanan Zong, Zirong Han, Wen Yang, Zhaoqun Liu, Lingling Wang, Linsheng SongAbstractAs a family of negatively feedback regulating factors, the suppressor of cytokine signaling (SOCS) can depress cytokine signal transduction, and eventually modulate growth, development, differentiation, and immune response. In the present study, a SOCS homologue (designated as CgSOCS6) was identified from oyster Crassostrea gigas. The open reading frame of CgSOCS6 cDNA was of 1167 bp encoding a peptide of 388 amino a...
Source: Fish and Shellfish Immunology - August 31, 2019 Category: Biology Source Type: research

Identification and characterization of novel type of RNAs, circRNAs in crucian carp Carassius auratus gibelio
In this study, the expression pattern of circRNA in Crucian carp was investigated with transcriptomic analysis. The results showed that only 37 circRNAs were identified from the Crucian carp, and these circRNAs biogenesis was formed with canonical GU-AG splicing mechanism with unevenly distributed on the chromosomes. Wherein, most of the circRNAs were derived from the sense overlapping strategy. Reverse transcript PCR and Sanger sequencing data indicated that these circRNAs were existed authenticity in Crucian carp. The bioinformatics analysis indicated that circRNAs identified from the Crucian carp with potential miRNA sp...
Source: Fish and Shellfish Immunology - August 31, 2019 Category: Biology Source Type: research

Effects of stress and cortisol on the polarization of carp macrophages
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Magdalena Maciuszek, Leszek Rydz, Iga Świtakowska, B.M. Lidy Verburg-van Kemenade, Magdalena ChadzińskaAbstractIn teleost fish, myelopoiesis is maintained both in the head (HK) and trunk kidney (TK), but only the HK holds the endocrine cells that produce the stress hormone cortisol. We now compared the effects of prolonged restraint stress (in vivo) and cortisol (in vitro) on the polarization of HK and TK-derived carp macrophages.Monocytes/macrophages from both sources were treated in vitro with cortisol, lipopolysaccharide or wit...
Source: Fish and Shellfish Immunology - August 31, 2019 Category: Biology Source Type: research

PPAR-δ of orange-spotted grouper exerts antiviral activity against fish virus and regulates interferon signaling and inflammatory factors
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Yuxin Wang, Yepin Yu, Qing Wang, Shina Wei, Shaowen Wang, Qiwei Qin, Min YangAbstractPeroxisome proliferator-activated receptor δ (PPAR-δ), also called PPAR-β or PPAR-β/δ, is a member of the peroxisome proliferator-activated receptor (PPAR) family, which belongs to the nuclear steroid receptor superfamily. Activated PPARs participate in the regulation of lipid and glucose metabolism and also affect cellular proliferation, differentiation, and apoptosis, and the immune responses. To investigate the roles o...
Source: Fish and Shellfish Immunology - August 31, 2019 Category: Biology Source Type: research

TcpA, a novel Yersinia ruckeri TIR-containing virulent protein mediates immune evasion by targeting MyD88 adaptors
In conclusion, our results showed that TcpA acted as a new virulence factor in Y. ruckeri could suppress innate immune response and increase virulence by inhibiting TLR and MyD88-mediated specific signaling, highlighting a novel strategy for innate immune evasion in bacteria. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - August 31, 2019 Category: Biology Source Type: research

Effect of CRISPR/Cas9-mediated knockout of either Mx1 or ISG15 gene in EPC cells on resistance against VHSV infection
In this study, to know the anti-viral hemorrhagic septicemia virus (VHSV) role of Mx1 and ISG15 of EPC cells, either Mx1 or ISG15 gene was knocked-out using a CRISPR/Cas9 system, and the progression of cytopathic effects (CPE) and viral growth were analyzed. Mx1 gene and ISG15 gene knockout EPC cells were successfully produced via CRISPR/Cas9 coupled with a single-cell cloning. Through the sequence analysis, one clone showing two heterozygous indel patterns in Mx1 gene and a clone showing three heterozygous indel patterns in ISG15 gene were selected for further analyses. Mx1 knockout EPC cells did not show any differences ...
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

Effects of potential probiotic Bacillus velezensis K2 on growth, immunity and resistance to Vibrio harveyi infection of hybrid grouper (Epinephelus lanceolatus♂ × E. fuscoguttatus♀)
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Jing Li, Zhi-Bin Wu, Zhao Zhang, Ji-Wei Zha, Shen-Ye Qu, Xiao-Zhou Qi, Gao-Xue Wang, Fei LingAbstractNowadays, there is no suitable treatment for vibriosis in groupers. So an eco-efficient and environmentally friendly treatment is necessary for the grouper industry. Probiotic-feeding has been a promising strategy to control the bacterial pathogens in aquaculture. A new Bacillus velezensis strain named K2 was isolated from the intestinal tract of healthy grouper, and exhibited wide antimicrobial spectrum of against fish pathogens, inc...
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

Two DExD/H-box helicases, DDX3 and DHX9, identified in rainbow trout are able to bind dsRNA
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Sarah J. Poynter, Shanee Herrington-Krause, Stephanie J. DeWitte-OrrAbstractIn mammals, the multifunctional DExH/D-box helicases, DDX3 and DHX9, are nucleic acid sensors with a role in antiviral immunity; their role in innate immunity in fish is not yet understood. In the present study, full-length DDX3 and DHX9 coding sequences were identified in rainbow trout (Oncorhynchus mykiss). Bioinformatic analysis demonstrated both deduced proteins were similar to those of other species, with ~80% identity to other fish species and ~70&ndash...
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

Bioflocs substituted fishmeal feed stimulates immune response and protects shrimp from Vibrio parahaemolyticus infection
In conclusion, the present study demonstrates that shrimp survival and immunity are enhanced by biofiocs substituted fishmeal. Significantly, the bioflocs diets activated the immune response to prevent V. parahaemolyticus infection. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

β-glucan modulates cortisol levels in stressed pacu (Piaractus mesopotamicus) inoculated with heat-killed Aeromonas hydrophila
In this study, we show that β-glucan can modulate cortisol release in fish. We simulated a common situation in aquaculture: the transport of fish followed by contact with an opportunistic pathogen and observed what effect glucan had on the immune and stress response in these conditions. Pacu (Piaractus mesopotamicus) were fed with a diet containing β-glucan (0.1%) for 15 days prior to transport followed by an injection with heat-killed Aeromonas hydrophila. We sampled fish before transport, at arrival and at 3 and 24 h after bacterial injection. β-Glucans are used in aquaculture and have a known immunostim...
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

In vivo administration of LPS and β-glucan generates the expression of a serum lectin and its cellular receptor in Cherax quadricarinatus
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): José Luis Sánchez-Salgado, Mohamed Alí Pereyra, Concepción Agundis, Montserrat Calzada-Ruiz, Erika Kantun-Briceño, Edgar ZentenoAbstractIn crustaceans, it has been suggested that specific protection against pathogens could be triggered by vaccines and biological response modifiers; although the specific mechanisms of this protection have not been clarified yet. In the crayfish Cherax quadricarinatus, a humoral lectin (CqL) binds its own granular hemocytes through a specific receptor (CqLR) and incr...
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

Functional characterization of IRF8 regulation of type II IFN in golden pompano (Trachinotus ovatus)
Publication date: November 2019Source: Fish & Shellfish Immunology, Volume 94Author(s): Ke-Cheng Zhu, Hua-Yang Guo, Nan Zhang, Bao-Suo Liu, Liang Guo, Shi-Gui Jiang, Dian-Chang ZhangAbstractInterferon regulatory factor 8 (IRF8) increases type I IFN transcription levels by binding to IFN promoters, thereby playing a role in innate immunity. Nevertheless, the detailed mechanism through which IRF8 regulates type II IFN in fish remains ambiguous. In the present study, two genes from the golden pompano (Trachinotus ovatus), IRF8 (ToIRF8) and IFN gamma (ToIFNγ), were identified in the IFN/IRF-based signalling pathway. ...
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

Dynamic immune and metabolism response of clam Meretrix petechialis to Vibrio challenge revealed by a time series of transcriptome analysis
This study provides a sight that the dynamic immunity and metabolic responses may be integrated to improve the host survival and shift more energy for immune defense. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

Isolation and expression of four Megalobrama amblycephala toll-like receptor genes in response to a bacterial infection
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Fan-Bin Zhan, Kianann Tan, Xiaoran Song, Jiongying Yu, Wei-Min WangAbstractToll-like receptors (TLRs) are a category of pattern recognition receptors (PRRs), which recognize pathogen associated molecular patterns (PAMPs) and participate in the immune responses. We identified tlr5a, tlr5b, tlr9 and tlr21 from the genome of blunt snout bream (Megalobrama amblycephala). All four tlrs were constitutively expressed in all examined tissues. After an immune bacterial challenge with Aeromonas hydrophila, their expressionwas up-regulated in l...
Source: Fish and Shellfish Immunology - August 29, 2019 Category: Biology Source Type: research

Genome organization and definition of the Penaeus monodon viral responsive protein 15 (PmVRP15) promoter
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Phattarunda Jaree, Taro Kawai, Chu-Fang Lo, Anchalee Tassanakajon, Kunlaya SomboonwiwatAbstractThe viral responsive protein 15 from the black tiger shrimp Penaeus monodon (PmVRP15) is a highly responsive gene upon white spot syndrome virus (WSSV) challenge. It is identified from hemocyte and important for WSSV trafficking and assembly. However, the knowledge of PmVRP15 gene regulation is limited. In the present study, the genome organization and 5′upstream promoter sequences of PmVRP15 gene were investigated. The PmVRP15 gene w...
Source: Fish and Shellfish Immunology - August 28, 2019 Category: Biology Source Type: research

Antimicrobial activity and mechanisms of multiple antimicrobial peptides isolated from rockfish Sebastiscus marmoratus
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Jun Bo, Ying Yang, Ronghui Zheng, Chao Fang, Yulu Jiang, Jie Liu, Mengyun Chen, Fukun Hong, Christyn Bailey, Helmut Segner, Kejian WangAbstractPathogenic disease is a major factor affecting the aquaculture of the rockfish Sebastiscus marmoratus, an important commercial species inhabiting the nearshore waters of the Western Pacific Ocean. Antimicrobial peptides (AMPs), as critical components of innate immunity, have been considered as promising antibiotic substitutes. The aims of this study were 1) to identify major AMPs in the rockfi...
Source: Fish and Shellfish Immunology - August 28, 2019 Category: Biology Source Type: research

Generation and functional evaluation of a DNA vaccine co-expressing Vibrio anguillarum VAA protein and flounder interleukin-2
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Hongsen Xu, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Wenbin ZhanAbstractIn our previous study, a DNA plasmid encoding the VAA gene of Vibrio anguillarum was constructed and demonstrated to confer moderated protection against V. anguillarum challenge. Here, a bicistronic DNA vaccine (pVAA-IRES-IL2), co-expressing the VAA gene of V. anguillarum and Interleukin-2 (IL2) gene of flounder, was constructed to increase the protective efficacy of VAA DNA vaccine. The potential of pVAA-IRES-IL2 to express both VAA and IL2 in transfected HINAE ...
Source: Fish and Shellfish Immunology - August 28, 2019 Category: Biology Source Type: research

Involvement of apoptosis in the dialogue between the parasite Bonamia ostreae and the flat oyster Ostrea edulis
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Ophélie Gervais, Bruno Chollet, Christine Dubreuil, Serena Durante, Chunyan Feng, Cyril Hénard, Cyrielle Lecadet, Delphine Serpin, Renault Tristan, Isabelle ArzulAbstractThe protozoan parasite Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. Control of shellfish diseases mostly relies on prevention measures including transfer restrictions and stock management measures such as breeding programmes. These prevention and mitigation measures require a b...
Source: Fish and Shellfish Immunology - August 25, 2019 Category: Biology Source Type: research

Transcriptomic analysis of Baltic cod (Gadus morhua) liver infected with Contracaecum osculatum third stage larvae indicates parasitic effects on growth and immune response
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Huria Marnis, Per W. Kania, Khairul Syahputra, Shaozhi Zuo, Ron P. Dirks, Kurt BuchmannAbstractHigh infection levels due to third-stage larvae of the anisakid nematode Contracaecum osculatum have been documented in cod from the eastern part of the Baltic sea during the latest decades. The nematode larvae mainly infect the liver of Baltic cod and prevalence of infection has reached 100% with a mean intensity up to 80 parasites per host in certain areas and size classes. Low condition factors of the cod have been observed concomitant w...
Source: Fish and Shellfish Immunology - August 25, 2019 Category: Biology Source Type: research

Identification and functional characterization of a C-type lectin gene from Litopenaeus vannamei that is associated with ER-stress response
In this study, we cloned a novel CTL gene (LvCTLU) from Litopenaeus vannamei. LvCTLU is involved in microbe agglutination and phagocytosis. Downregulating LvCTLU increased the cumulative mortality of L. vannamei after Vibrio parahemolyticus infection. Similar to other reported CTLs, LvCTLU also had antiviral properties. Downregulation of LvCTLU also increased the cumulative mortality of L. vannamei after infection with white spot syndrome virus. More importantly, LvCTLU expression was induced by the unfolded protein response (UPR), which is the key pathway in the endoplasmic reticulum (ER)-stress response of eukaryotic org...
Source: Fish and Shellfish Immunology - August 25, 2019 Category: Biology Source Type: research

Multiple subtypes of TLR22 molecule from Schizothorax prenanti present the functional diversity in ligand recognition and signal activation
In this study, we cloned and identified three TLR22 molecules from Schizothorax prenanti (S. prenanti), named as spTLR22-1, spTLR22-2 and spTLR22-3. The full-length coding regions of spTLR22s are 2841 bp, 2805 bp and 2868 bp and coding 946 aa, 934 aa and 955 aa, respectively. All spTLR22s are composed of multiple leucine-rich repeat (LRR) domains, a transmembrane structure and a Toll/IL-1 receptor (TIR) region. The phylogenetic analysis showed that three spTLR22s were close to Cyprinus carpio TLR22-1, TLR22-2 and TLR22-3, respectively. Among the spTLR22s, they presented not close relationship but remained to belong to TLR2...
Source: Fish and Shellfish Immunology - August 25, 2019 Category: Biology Source Type: research

HSP40 gene family in pearl oyster Pinctada fucata martensii: Genome-Wide identification and function analysis
In conclusion, all these results provide a comprehensive basis for the further analysis of PmHSP40 function in pearl oysters. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - August 23, 2019 Category: Biology Source Type: research

Effects of dietary supplementation of bovine lactoferrin on antioxidant status, immune response and disease resistance of yellowfin sea bream (Acanthopagrus latus) against Vibrio harveyi
This study investigated the effect of the dietary supplementation of bovine lactoferrin (LF) on growth performance, hematological and immunological parameters, antioxidant enzymes activity and disease resistance against Vibrio harveyi in yellowfin sea bream (Acanthopagrus latus) fingerling. The fish with initial body weight 10 ± 0.3 g were randomly distributed at 10 fish per each 250 L fiberglass tank, and fed with four experimental diets (a control basal diet and three supplemented diets with 400, 800 and 1200 mg LF kg−1 diet) for 8 weeks. The obtained results showed that fish fed with LF suppleme...
Source: Fish and Shellfish Immunology - August 23, 2019 Category: Biology Source Type: research

Development of an attenuated oral vaccine strain of tilapia Group B Streptococci serotype Ia by gene knockout technology
In this study, a Δ2 mutant that deletion of D2 fragment in parental virulent strain HN016 was constructed, and the safety, stability, immunogenicity, and growth characteristics, as well as the virulence mechanism of Δ2 mutant were evaluated. The results showed that Δ2 mutant was not pathogenic to tilapia, and the virulent revertants were not observed after 50 generations of passage. The RPS reached 96.11% at 15 days and 93.05% at 30 days, respectively, after intraperitoneal injection, while RPS reached 74.80% at 15 days and 53.16% at 30 days, respectively, after oral immunization. The growth of Δ2 m...
Source: Fish and Shellfish Immunology - August 23, 2019 Category: Biology Source Type: research

Transcriptomic analysis of clam extrapallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development
This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a downregulation of transcripts implied in immune functions (lysosomal activity and complement- and lectin-dependent PRR pathways) was observed during infection. We also showed a deregulation of transcripts encoding proteins involved in the actin cytoskeleton organization such as an...
Source: Fish and Shellfish Immunology - August 23, 2019 Category: Biology Source Type: research

Dual RNA-seq uncovers the immune response of Larimichthys crocea to the secY gene of Pseudomonas plecoglossicida from the perspective of host-pathogen interactions
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Luying Wang, Yunjia Sun, Lingmin Zhao, Xiaojin Xu, Lixing Huang, Yingxue Qin, Yongquan Su, Jiaonan Zhang, Qingpi YanAbstractPseudomonas plecoglossicida is a Gram-negative aerobic bacterium that causes high mortality and serious economic losses in some commercial marine fish. Expression of secY was found to be significantly upregulated at 18 °C compared to 28 °C by RNA-seq and qRT-PCR. All five tested recombinant vectors (pCM130/tac + shRNA) significantly reduced secY mRNA levels in P. plecoglossicida. The ...
Source: Fish and Shellfish Immunology - August 23, 2019 Category: Biology Source Type: research

Protective effect of in-feed specific IgM towards Yersinia ruckeri in rainbow trout
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Jiwan K. Chettri, Azmi Al-Jubury, Marie B. Hansen, Allan Lihme, Inger Dalsgaard, Kurt Buchmann, Peter M.H. HeegaardAbstractTightened regulations and an environmentally friendly approaches in fish production have greatly reduced the use of antibiotics but green solutions are continuously being explored. The use of functional feed may have a potential in the aquaculture sector in securing biomass and minimizing the loss from disease. In the present study, we tested the concept that blood from the fish slaughterhouse can be used for mas...
Source: Fish and Shellfish Immunology - August 23, 2019 Category: Biology Source Type: research

Immersion immunization with recombinant baculoviruses displaying cyprinid herpesvirus 2 membrane proteins induced protective immunity in gibel carp
In this study, nine truncated CyHV-2 membrane glycoproteins (ORF25, ORF25C, ORF25D, ORF30, ORF124, ORF131, ORF136, ORF142A, ORF146) and a GFP reporter protein were respectively expressed using baculovirus surface displaying system. Western blot showed that the proteins were successfully packaged in the recombinant virus particles. In baculovirus transduced gibel carp kidney cells, the target proteins were expressed and displayed on the fish cell surface. Healthy gibel carp were immunized by immersion with the recombinant baculoviruses and the fish treated with phosphate-buffered saline (PBS) were served as mock group. The ...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

Transcriptome sequencing of hybrid bester sturgeon: Responses to poly (I:C) in the context of comparative immunogenomics
We report transcriptome sequencing of gill, head kidney, and spleen of bester sturgeon (a hybrid of beluga Huso huso and sterlet Acipenser ruthenus) injected with synthetic double-stranded RNA (polyI:C). The composition of transcriptome and responses to treatment were examined in the context of comparative genomics with focus on immune genes. Sturgeon transcripts matched to 21.5 k different proteins (blastx). With reference to Atlantic salmon, the functional groups and pathways of the immune system were uniformly represented: at average 36.5 ± 0.8% genes were found. Immune genes comprise a significant fraction o...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

Atlantic salmon (Salmo salar) mounts systemic and mucosal stress responses to peracetic acid
In conclusion, the results show that salmon were able to mount a robust adaptive response to different PAA doses and exposure times, and a combined exposure to stress and PAA. These results underscore the potential of PAA as a chemotherapeutant for salmon at PAA concentrations commonly applied to control parasitic infestations. (Source: Fish and Shellfish Immunology)
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

The growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia affected by dietary Rhodiola rosea polysaccharide
This study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia. RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses. Three diets supplemented with three different levels of RRP (0.2, 0.6 and 1 g kg diet−1) were formulated and tested for growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkii, while a diet without any RRP supplementation served as control. After 8 weeks of feeding, body we...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

Is related the hematopoietic stem cells differentiation in the Nile tilapia with GABA exposure?
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Armando Vega-López, Nataraj S. Pagadala, Brenda P. López-Tapia, Ruth L. Madera-Sandoval, Erika Rosales-Cruz, Minerva Nájera-Martínez, Elba Reyes-MaldonadoAbstractThe signaling mediated by small non-proteinogenic molecules, which probably have the capacity to serve as a bridge amongst complex systems is one of the most exiting challenges for the study. In the current report, stem cells differentiation of the immune system in Nile tilapia treated with sub-basal doses of GABA evaluated as c-kit+ and Sca-1+ ce...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

Identification of cells expressing two peptidoglycan recognition proteins in the gill of the vent mussel, Bathymodiolus septemdierum
In this study, we focused on a group of highly conserved PRRs, peptidoglycan recognition proteins (PGRPs), in the deep-sea mussel, Bathymodiolus septemdierum, which harbours chemosynthetic bacteria in their gill epithelial cells. We isolated B. septemdierum PGRP genes BsPGRP-S and BsPGRP-L, which encode a short- and a long-type PGRP, respectively. The short-type PGRP has a signal peptide and was expressed in the asymbiotic goblet mucous cells in the gill epithelium, whereas the long-type PGRP was predicted to include a transmembrane domain and was expressed in gill bacteriocytes. Based on these findings, we hypothesize tha...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

A novel calreticulin-related molecule that interacts with bacteria and enhances host resistance against bacterial infection in black rockfish, Sebastes schlegeli
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Guang-hua Wang, Zhao-xia Li, En-mian Guo, Jing-jing Wang, Min Zhang, Yong-hua HuAbstractCalreticulin (CRT) is a highly conserved and multi-functional protein with diverse localizations. CRT has lectin-like properties and possesses important immunological activities in mammalian. In teleost, very limited studies on CRT immunologic function have been documented. In the present study, a CRT homologue (SsCRT) was cloned, identified and characterized from black rockfish, Sebastes schlegeli, an important aquaculture species in East Asia. T...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

Biochemical and biological characterization of the Hypanus americanus mucus: A perspective on stingray immunity and toxins
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Guilherme Rabelo Coelho, Pedro Prezotto Neto, Fernanda Cortinhas Barbosa, Rafael Silva Dos Santos, Patrícia Brigatte, Patrick Jack Spencer, Sandra Coccuzzo Sampaio, Fernanda D’Amélio, Daniel Carvalho Pimenta, Juliana Mozer ScianiAbstractStingrays skin secretions are largely studied due to the human envenoming medical relevance of the sting puncture that evolves to inflammatory events, including necrosis. Such toxic effects can be correlated to the biochemical composition of the sting mucus, according to the litera...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

A bactericidal permeability-increasing protein (BPI) from manila clam Ruditapes philippinarum: Investigation on the antibacterial activities and antibacterial action mode
Publication date: October 2019Source: Fish & Shellfish Immunology, Volume 93Author(s): Dinglong Yang, Yijing Han, Lizhu Chen, Ruiwen Cao, Qing Wang, Zhijun Dong, Hui Liu, Xiaoli Zhang, Qianqian Zhang, Jianmin ZhaoAbstractBactericidal permeability-increasing protein (BPI) is an antimicrobial protein with potent endotoxin-neutralising activity and plays a crucial role in innate immunity against bacterial infection. In the present study, a bpi (designed as rpbpi) was identified and characterized from manila clam Ruditapes philippinarum. Multiple alignments and phylogenetic analysis suggested that rpbpi was a new member of...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research

Comparative proteomic investigation of Marsupenaeus japonicus hepatopancreas challenged with Vibrio parahaemolyticus and white spot syndrome virus
This study aimed to use isobaric tags (IBTs) to investigate the immune response of the hepatopancreas of Marsupenaeus japonicas infected with Vibrio parahaemolyticus or white spot syndrome virus (WSSV). Liquid chromatography-tandem mass spectrometry and protein sequencing identified 1005 proteins. Among them, 109 proteins were upregulated and 94 were downregulated after V. parahaemolyticus infection. After WSSV infection, 130 proteins were identified as differentially abundant, including 88 that were upregulated and 42 were downregulated. Fifty-four proteins were identified as differentially abundant after both V. parahaem...
Source: Fish and Shellfish Immunology - August 22, 2019 Category: Biology Source Type: research