Corrigendum to ’ 5-Aza-dC promotes T-cell acute lymphoblastic leukemia cell invasion via downregulation of DNMT1 and upregulation of MMP-2 and MMP-9’ [Volume 114, October 2022, Pages 43-53.e2]
Authors declare that following the publication of the original article, due to the discontinuation of author collaboration, the following Co-Authors requested to be removed as Authors from this article: Ting Yang**, Jingjing Xu ⁎⁎, Nan Li ⁎⁎, Qian Li **, Minmin Chen **, Kangni Lin **, Haojie Zhu **, Shaozhen Chen ** and Rong Zheng **. The correct Author line is shown above. The request has been approved by all Authors of the original article. (Source: Experimental Hematology)
Source: Experimental Hematology - August 5, 2023 Category: Hematology Authors: Congmeng Lin, Yongxin Xie, Wenwen Huang, Dayi Lin, Luhui Lin Tags: Corrigendum Source Type: research
Expanding hematopoietic stem cell ex vivo: recent advances and technical considerations
Self-renewing hematopoietic stem cells (HSCs) are a fundamental cellular component in the bone marrow (BM) that enables long-term hematopoietic reconstitution upon transplantation. Since the experimental demonstration of the existence and their long-term repopulating potential of HSCs by James E. Till and Ernst A. McCulloh1,2, self-renewing mechanisms in HSCs have been the subject of intensive investigation. In particular, the BM microenvironment has been demonstrated to play a crucial role in mediating HSC self-renewal. (Source: Experimental Hematology)
Source: Experimental Hematology - August 3, 2023 Category: Hematology Authors: Juan A. Rubio-Lara, Kyomi J. Igarashi, Shubhankar Sood, Alban Johansson, Pia Sommerkamp, Masayuki Yamashita, Dawn S. Lin Tags: Perspective Source Type: research
Cover 2: Editorial Board
(Source: Experimental Hematology)
Source: Experimental Hematology - July 28, 2023 Category: Hematology Source Type: research
Good prognosis of adult hemophagocytic lymphohistiocytosis associated with the germline HAVCR2 mutation
Hemophagocytic lymphohistiocytosis (HLH) is a potentially fatal systemic condition caused by hyperactivation of macrophages, cytotoxic T cells, and natural killer (NK) cells, which release excessive cytokines, leading to inflammation and organ damage [1,2]. HLH can be categorized as primary HLH, resulting from an inherited defect, and secondary HLH, caused by malignancies, infections, autoimmune diseases, or unidentified causes. Primary or familial HLH occurs in the presence of an underlying predisposing genetic defect in immune function, including mutations in perforin 1, UNC-13 homolog D, RAS-related protein Rab-27A, syn...
Source: Experimental Hematology - July 18, 2023 Category: Hematology Authors: Pitchayut Boonyabaramee, Chantana Polprasert, Sirorat Kobbuaklee, Rung Settapiboon, Saranya Pongudom, Saruta Faknuam, Sunisa Kongkiatkamon, Kitsada Wudhikarn, Ponlapat Rojnuckarin Tags: Brief Communication Source Type: research
Good prognosis of adult hemophagocytic lymphohistiocytosis (HLH) associated with the germline HAVCR2 mutation
Hemophagocytic lymphohistiocytosis (HLH) is a potentially fatal systemic condition caused by hyperactivation of macrophages, cytotoxic T cells and natural killer (NK) cells, which release excessive cytokines leading to inflammation and organ damage.1, 2 HLH can be categorized as primary HLH resulting from an inherited defect, while secondary HLH is caused by malignancies, infections, autoimmune diseases or unidentified causes. Primary or familial HLH occurs in the presence of an underlying predisposing genetic defect in immune function including mutation in perforin 1 (PRF1), UNC-13 homolog D (UNC13D), RAS-related protein ...
Source: Experimental Hematology - July 18, 2023 Category: Hematology Authors: Pitchayut Boonyabaramee, Chantana Polprasert, Sirorat Kobbuaklee, Rung Settapiboon, Saranya Pongudom, Saruta Faknuam, Sunisa Kongkiatkamon, Kitsada Wudhikarn, Ponlapat Rojnuckarin Tags: Brief Communication Source Type: research
Effects of ruxolitinib on murine regulatory T cells are immune-context dependent
Aplastic anemia (AA) is a bone marrow failure (BMF) disorder mediated by immune dysfunction resulting in pancytopenia and marrow hypoplasia. To study immune AA experimentally, BMF can be induced in mice with major histocompatibility complex-mismatched lymph node (LN) cell infusion after sublethal total body irradiation (TBI). Based on the suppressive effect of the JAK 1/2 inhibitor ruxolitinib (RUX) on T-cell activation and inflammatory cytokine production, we previously studied RUX in our murine BMF model. (Source: Experimental Hematology)
Source: Experimental Hematology - July 16, 2023 Category: Hematology Authors: Nidhi Aggarwal, Ash Lee Manley, Jichun Chen, Emma M. Groarke, Xingmin Feng, Neal S. Young Tags: Brief Communication Source Type: research
Effects of Ruxolitinib on Murine Regulatory T Cells Are Immune Context Dependent
Aplastic anemia (AA) is a bone marrow failure (BMF) disorder mediated by immune dysfunction resulting in pancytopenia and marrow hypoplasia. To study immune AA experimentally, BMF can be induced in mice with major histocompatibility complex-mismatched lymph node (LN) cell infusion after sublethal total body irradiation (TBI). Based on the suppressive effect of the JAK 1/2 inhibitor ruxolitinib (RUX) on T cell activation and inflammatory cytokine production, we previously studied RUX in our murine BMF model. (Source: Experimental Hematology)
Source: Experimental Hematology - July 16, 2023 Category: Hematology Authors: Nidhi Aggarwal, Ash Lee Manley, Jichun Chen, Emma M. Groarke, Xingmin Feng, Neal S. Young Tags: Brief Communication Source Type: research
New insight into the causes, consequences, and correction of hematopoietic stem cell aging
The human lifespan has doubled over the past 200 years reflecting significant progress in sanitation and medicine [1]. However, this increase in lifespan presents us with new socioeconomic and medical challenges. Somatic stem cells, including hematopoietic stem cell (HSCs), are tasked with maintaining, repairing, and replenishing tissues throughout life. Thus, their lifespan, unlike the bulk of cells that make up the human body, equals or exceeds the lifespan of the organism [2,3]. This responsibility for regeneration in combination with their uniquely long lifespan confers vulnerability in the system: age-related decline ...
Source: Experimental Hematology - July 8, 2023 Category: Hematology Authors: Els Mansell, Dawn S. Lin, Stephen J. Loughran, Michael D. Milsom, Jennifer J. Trowbridge Tags: Review Source Type: research
New Insight into the Causes, Consequences and Correction of Hematopoietic Stem Cell Ageing
The human lifespan has doubled over the past 200 years reflecting significant progress in sanitation and medicine (1). However, this increase in lifespan presents us with new socio-economic and medical challenges. Somatic stem cells including HSCs are tasked with maintaining, repairing and replenishing tissues throughout life. Thus, their lifespan, unlike the bulk of cells that make up the human body, equals or exceeds the lifespan of the organism (2, 3). This responsibility for regeneration in combination with their uniquely long lifespan confers vulnerability in the system: age-related decline of stem cell fitness direct...
Source: Experimental Hematology - July 8, 2023 Category: Hematology Authors: Els Mansell, Dawn S Lin, Stephen J Loughran, Michael D Milsom, Jennifer J Trowbridge Tags: Review Source Type: research
Critical role of MXRA7 in differentiation blockade in human acute promyelocytic leukemia cells
Since the accomplishment of Human Genome Project in 2002 and with the advancement of various high-throughput research strategies, characterization of previously ill-defined genes or discovery of novel functions of known genes has been accelerated, which in turn facilitates the discovery of the pathogenesis of or effective therapies for diseases. In the past decade, we investigated the potential role of a less-cared gene, matrix remodeling associated 7 (MXRA7), in different pathophysiological processes including leukemia. (Source: Experimental Hematology)
Source: Experimental Hematology - July 5, 2023 Category: Hematology Authors: Zhenjiang Sun, Dandan Lin, Ying Shen, Kunpeng Ma, Benfang Wang, Hong Liu, Suning Chen, Depei Wu, Yiqiang Wang Tags: Article Source Type: research
Cover 2: Editorial Board
(Source: Experimental Hematology)
Source: Experimental Hematology - June 26, 2023 Category: Hematology Source Type: research
Distinct roles of the preparatory and payoff phases of glycolysis in hematopoietic stem cells
Most adult hematopoietic stem cells (HSCs) maintain a quiescent state in physiological conditions [1, 2], and quiescence is closely related to HSC function [3]. Energy metabolism plays a crucial role in maintaining HSC quiescence [4, 5], shielding HSCs from accumulation of DNA damage and diminishment of repopulation capacity. HSCs are more metabolically inactive than progenitors, exemplified by low adenosine-triphosphate (ATP) production, low protein synthesis rate [6], low mTORC activity accompanied by high nuclear FOXO levels [7, 8], high autophagic activity upon metabolic stress [9-11], dependence on fatty acid oxidatio...
Source: Experimental Hematology - June 18, 2023 Category: Hematology Authors: Kohei Shiroshita, Hiroshi Kobayashi, Shintaro Watanuki, Daiki Karigane, Yuriko Sorimachi, Shinpei Tamaki, Miho Haraguchi, Masamichi Yamamoto, Ayako Nakamura-Ishizu, Shinichiro Okamoto, Keisuke Kataoka, Keiyo Takubo Tags: Article Source Type: research
BCR-ABL promotes hematopoietic stem and progenitor cell formation in embryonic stem cells
Hematopoietic Stem Cells (HSCs) ensure the continuous formation of blood cells their self-renewal and differentiation properties. Pluripotent Stem Cells (PSCs), including Embryonic Stem Cells (ESCs) and induced pluripotent stem cells (iPSCs), are characterized by their ability to generate all embryonic cell lineages. Generating HSCs from PSCs would provide a powerful paradigm to model normal and malignant hematopoiesis and engineer cell-based therapies. However, the identification of true long-term HSCs with both self-renewal and multipotent differentiation abilities from PSCs remains a major challenge. (Source: Experimental Hematology)
Source: Experimental Hematology - June 16, 2023 Category: Hematology Authors: J érôme Artus, Alina Zenych, Isidora Simanic, Christophe Desterke, Denis Clay, Sonia Saïm, Yousef Ijjeh, Lucas Eduardo Botelho de Souza, Sabrina Coignard, Annelise Bennaceur-Griscelli, Ali G. Turhan, Adlen Foudi Tags: Article Source Type: research
The YEATS Domain Epigenetic Reader Proteins ENL and AF9 and Their Therapeutic Value in Leukemia
The MLLT1 gene, which encodes the eleven-nineteen-leukemia (ENL) protein, is located on chromosome 19p13.3 and shares high sequence homology with its human paralog, MLLT3 (encoding the AF9 protein). Over thirty years ago, the discovery of the t(11;19) (q23;p13.3) (MLL1-MLLT1) and t(9;11) (p22;q23) (MLL1-MLLT3) translocations marked our introduction to these proteins as two of the most common MLL fusion partners, representing roughly 13% and 19% of the MLL-rearranged (MLL-r) cases across all ages [1 –4] (Figure 1A). (Source: Experimental Hematology)
Source: Experimental Hematology - June 6, 2023 Category: Hematology Authors: Hsiangyu Hu, Andrew G. Muntean Tags: Perspective Source Type: research
Assay optimization for the objective quantification of human multilineage colony-forming units
The colony-forming unit (CFU) assay can be used to assess the lineage potential of hematopoietic stem and progenitor cells (HSPCs) in vitro. CFU assays are routinely carried out using standardized commercially available products, such as methylcellulose-based MethoCult from STEMCELL Technologies [1]. Classification of HSPC lineage potential from multipotent progenitors has traditionally been accomplished by assessing colony morphology using brightfield light microscopy. Results are sometimes validated using low-throughput methods, such as in situ immunohistochemistry of colonies, cytospins, or flow cytometry of individuall...
Source: Experimental Hematology - June 2, 2023 Category: Hematology Authors: Evrett N. Thompson, Maximillian J. Carlino, Vanessa Scanlon, H. Leighton Grimes, Diane S. Krause Tags: Methods and Techniques Source Type: research
