Characterization of human engraftment and hemophagocytic lymphohistiocytosis in NSG-SGM3 neonate mice engrafted with purified CD34+ hematopoietic stem cells
“Humanized’ mice have been utilized as a novel approach for studies of basic human immunology, immuno-oncology, study of hematological disorders, infectious diseases, and autoimmunity. NSG (NOD.CB17-Prkdcscid Il2rgtm1Wj1/Sz) mice are commonly used recipients for human HSC1, 2. In addition to bei ng homozygous for the severe combined immunodeficiency (SCID) mutation, NSG mice also express a disrupted murine Interleukin (IL)2-receptor common γ chain which prevents B, T and NK cell development, along with many other immune lineages3. (Source: Experimental Hematology)
Source: Experimental Hematology - December 3, 2023 Category: Hematology Authors: O'Brien Liam, Walpole Carina, Leal-Rojas Ingrid, Shatunova Svetlana, Moore Andrew, Ingrid G Winkler, Guillerey Camille, Kristen J Radford Tags: Brief Communication Source Type: research
IFC Editorial Board
(Source: Experimental Hematology)
Source: Experimental Hematology - November 30, 2023 Category: Hematology Source Type: research
Understanding genetic heterogeneity in gene-edited hematopoietic stem cell products
The advent of gene editing has ushered in significant advances in biomedical research due to the ability to precisely manipulate genomes in live cells, with potential clinical applications emerging continually. Hematopoietic stem cells (HSCs) have been among the first primary cell types to undergo gene editing and for good reason. Their position at the top of the hematopoietic hierarchy, extensive phenotypic characterization, and the established practice of stem cell transplantation in the lab and in the clinic makes them ideal targets for genetic interventions. (Source: Experimental Hematology)
Source: Experimental Hematology - November 28, 2023 Category: Hematology Authors: Hans Jiro Becker, Satoshi Yamazaki Tags: Review Source Type: research
Beyond the horizon: Emerging therapeutic approaches in Myelodysplastic Neoplasms
Management of Myelodysplastic Neoplasms (MDS) presents a complex challenge and should always be tailored to each patient's individual case, focusing on both quality of life and extension of lifespan. The International Prognostic Scoring System-Revised (IPSS-R) has been a cornerstone in the risk stratification and management of MDS. It provides a comprehensive framework for predicting survival and the risk of transformation to acute myeloid leukemia (AML), based on five key parameters: cytogenetics, bone marrow blast percentage, and levels of hemoglobin, platelets, and absolute neutrophil count1. (Source: Experimental Hematology)
Source: Experimental Hematology - November 28, 2023 Category: Hematology Authors: Almuth Maria Anni Merz, Uwe Platzbecker Source Type: research
Understanding Genetic Heterogeneity in Gene-Edited HSC Products
CRISPR/Cas gene editing has transformed genetic research and is poised to drive the next generation of gene therapies targeting hematopoietic stem cells (HSCs). However, the installation of the ‘desired’ edit is most often only achieved in a minor subset of alleles. The array of cellular pathways triggered by gene editing tools produces a broad spectrum of ‘undesired’ editing outcomes, including short insertions and deletions (indels) and chromosome rearrangements, leading to consi derable genetic heterogeneity in gene edited HSC populations. (Source: Experimental Hematology)
Source: Experimental Hematology - November 28, 2023 Category: Hematology Authors: Hans Jiro Becker, Satoshi Yamazaki Tags: Review Source Type: research
Establishment of ganglioside GD2-expressing extranodal NK/T-cell lymphoma cell line with scRNA-seq analysis
Extranodal natural killer (NK)/T-cell lymphoma, nasal type (ENKL), is characterized by Epstein-Barr virus (EBV) infection and extremely poor survival [1 –9]. ENKL is common in South America and Asia; in East Asia it represents 3%–10% of all lymphomas [6]. Systemic dissemination, including bone marrow infiltration, is found at diagnosis in ∼35% of ENKL patients [8], in whom anthracycline-containing regimens yield dismal outcomes [1–9]. ENKL c ells often express multidrug resistance 1 protein/P-glycoprotein (encoded by MDR1/ABCB1) at high levels, with active efflux of various chemotherapeutic agents, including anthra...
Source: Experimental Hematology - November 27, 2023 Category: Hematology Authors: Shoko Sato, Midori Ishii, Kota Tachibana, Yoshiki Furukawa, Tokuko Toyota, Shintaro Kinoshita, Yoko Azusawa, Jun Ando, Miki Ando Tags: Article Source Type: research
Establishment of ganglioside GD2-expressing extranodal NK/T cell lymphoma cell line with scRNA-seq analysis
Extranodal NK/T-cell lymphoma, nasal type (ENKL), is characterized by Epstein-Barr virus (EBV) infection and extremely poor survival 1-9. ENKL is common in South America and Asia; in East Asia it represents 3-10% of all lymphomas 6. Systemic dissemination, including bone marrow infiltration, is found at diagnosis in ∼35% of ENKL patients 8, in whom anthracycline-containing regimens yield dismal outcomes 1-9. ENKL cells often express multidrug resistance 1 protein / P-glycoprotein (encoded by MDR1 / ABCB1) at high levels, with active efflux of various chemotherapeutic agents, including anthracyclines 10. (Source: Experimental Hematology)
Source: Experimental Hematology - November 27, 2023 Category: Hematology Authors: Shoko Sato, Midori Ishii, Kota Tachibana, Yoshiki Furukawa, Tokuko Toyota, Shintaro Kinoshita, Yoko Azusawa, Jun Ando, Miki Ando Tags: Article Source Type: research
Transcriptional and Functional Consequences of Oncostatin M Signaling on Young Dnmt3a-Mutant Hematopoietic Stem Cells
The process of aging has a profound impact on tissues and cells throughout the body. In the hematopoietic stem and progenitor cell (HSPC) compartment, aging is accompanied by acquisition of somatic mutations. Given the long-lived nature of the HSPC pool, these mutations can be propagated for many cellular generations and through most mature hematopoietic cell progeny. While most of these somatic mutations are considered ‘neutral’, a subset can confer a selective growth advantage to HSPCs, leading to a condition termed age-associated clonal hematopoiesis (CH). (Source: Experimental Hematology)
Source: Experimental Hematology - November 22, 2023 Category: Hematology Authors: Logan S. Schwartz, Kira A. Young, Timothy M. Stearns, Nathan Boyer, Kristina D. Mujica, Jennifer J. Trowbridge Tags: Article Source Type: research
Amyloid deposition through endocytosis in vascular endothelial cells
Primary amyloidosis (AL amyloidosis) is a disease form of amyloid deposition in multiple organs in plasma cell dyscrasias that produce amyloidogenic monoclonal immunoglobulin free light chains. The heart is the most vulnerable organ in this disease because amyloidogenic light chain proteins directly cause cardiomyocyte dysfunction and apoptosis independent of amyloid fibril deposition [1]. In addition, the deposition of amyloid fibrils in multiple organs causes cell damage and tissue architecture distortion resulting in organ dysfunction and poor prognosis (approximately 30% survival probability in 10 years from diagnosis ...
Source: Experimental Hematology - November 10, 2023 Category: Hematology Authors: Seiji Nishikage, Akira Fujisawa, Hiromi Endoh, Hirotaka Sakamoto, Tomohide Suzuki, Maki Kanzawa, Shinichi Ishii, Mitsumasa Okano, Eriko Nitta, Kimikazu Yakushijin, Hidesaku Asakura, Kandai Nozu, Ryo Nitta, Yoshio Katayama, Kazuhiko Sakaguchi Tags: Brief communication Source Type: research
Inhibitory effects of SARS-CoV-2 Spike protein and BNT162b2 vaccine on erythropoietin-induced globin gene expression in erythroid precursor cells (ErPCs) from β-thalassemia patients
Since its spread at beginning of 2020, the coronavirus disease 2019 (COVID-19) pandemic represents one of the major health problems, causing radical changes in the society behavior and forcing research efforts to develop novel therapeutic options [1 –3]. Several studies have been focused on possible effects of SARS-CoV-2 infection on hematological parameters [4–7]. Following the approval of the anti-SARS-CoV-2 vaccines by the Regulatory Agencies (EMA in Europe and FDA in United States) COVID-19 vaccines have been extensively tested and dist ributed worldwide [8–10]. (Source: Experimental Hematology)
Source: Experimental Hematology - November 6, 2023 Category: Hematology Authors: Lucia Carmela Cosenza, Giovanni Marzaro, Matteo Zurlo, Jessica Gasparello, Cristina Zuccato, Alessia Finotti, Roberto Gambari Tags: Article Source Type: research
Germline MPL mutations may be a rare cause of “triple-negative” thrombocytosis
Isolated thrombocytosis, defined as a platelet (PLT) count higher than 450 ×109/L, is mainly secondary to reactive conditions or, more rarely, a consequence of primary disorders arising in the bone marrow. The typical primary thrombocytosis is essential thrombocythemia (ET), a clonal acquired disorder belonging to Philadelphia-negative myeloproliferative neoplasms (MPN). In more than 90% of cases, ET is driven by JAK2 V617F mutation or by somatic mutations in exon 9 of CALR or in exon 10 of MPL, while the remaining cases are termed “triple-negative”. (Source: Experimental Hematology)
Source: Experimental Hematology - November 6, 2023 Category: Hematology Authors: Oscar Borsani, Daniela Pietra, Ilaria Carola Casetti, Daniele Vanni, Giacomo Riccaboni, Silvia Catrical à, Bossi Grazia, Emanuela Boveri, Luca Arcaini, Elisa Rumi Tags: Article Source Type: research
Editorial: Metabolic control in hematopoietic homeostasis, Edited by Keisuke Ito
Recent advances in metabolic assessments, genetic models and imaging techniques have led to rapidly accumulating new evidence regarding the metabolic requirements of hematopoietic homeostasis; however, it remains unclear precisely how specific profiles of metabolism control hematopoiesis. (Source: Experimental Hematology)
Source: Experimental Hematology - November 4, 2023 Category: Hematology Authors: Dr Ito Keisuke Tags: Editorial Source Type: research
Editorial: Articles from the Special Issue on Metabolic control in hematopoietic homeostasis; Edited by Keisuke Ito
Recent advances in metabolic assessments, genetic models and imaging techniques have led to rapidly accumulating new evidence regarding the metabolic requirements of hematopoietic homeostasis; however, it remains unclear precisely how specific profiles of metabolism control hematopoiesis. (Source: Experimental Hematology)
Source: Experimental Hematology - November 4, 2023 Category: Hematology Authors: Dr Ito Keisuke Tags: Editorial Source Type: research
Prognostic Significance of Myeloid-derived Suppressor Cells and Systemic Inflammation in Newly Diagnosed Diffuse Large B-Cell Lymphoma Treated with Chemoimmunotherapy
Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma in the United States. Approximately 60% of patients diagnosed with this disease achieve long-term remission with frontline chemoimmunotherapy; however, patients who relapse face poor prognosis.[1] Current prognostic indices such as the International Prognostic Index (IPI) and its revised version (R-IPI), which primarily rely on clinical variables,[2] have greatly improved outcome prediction for patients with DLBCL treated with standard chemoimmunotherapy. (Source: Experimental Hematology)
Source: Experimental Hematology - October 28, 2023 Category: Hematology Authors: David M. Foureau, Fei Guo, Nury M Steuerwald, Lawrence J Druhan, Belinda R Avalos, Edward Copelan, Danyu Sun, Bei Hu, Tamara Moyo, Ryan Jacobs, Steven Park, Nilanjan Ghosh Tags: Brief Communication Source Type: research
Cover 2: Editorial Board
(Source: Experimental Hematology)
Source: Experimental Hematology - October 26, 2023 Category: Hematology Source Type: research
