Cell fate decision in erythropoiesis: insights from multi-omics studies.
Erythropoiesis is an important cellular differentiation process that leads to the formation of red blood cells from hematopoietic stem cells (HSCs) [1,2]. Owing to sophisticated mouse models [3] and human ex vivo differentiation systems that recapitulate all steps of differentiation [4], erythropoiesis has been comprehensibly analyzed, which makes it an ideal model system to address outstanding questions in biology. For example, enhancers were first characterized through extensive analyses of transcription at the ß-globin locus during erythroid differentiation [5]. (Source: Experimental Hematology)
Source: Experimental Hematology - January 20, 2024 Category: Hematology Authors: Steven Tur, Carmen G. Palii, Marjorie Brand Source Type: research
Computing Sickle Erythrocyte Health Index on quantitative phase imaging and machine learning
This study developed an in vitro assessment, the Sickle Erythrocyte Health Index, using quantitative phase imaging (QPI) and machine learning to model the health of RBCs in people with SCD. The health index combines assessment of cell deformation, sickle-shaped classification, and membrane flexibility to evaluate erythrocyte health. (Source: Experimental Hematology)
Source: Experimental Hematology - January 19, 2024 Category: Hematology Authors: Yaw O.N. Ansong-Ansongton, Timothy D. Adamson Tags: Nonmalignant hematologic diseases Source Type: research
Computing Sickle Erythrocyte Health Index Based on Quantitative Phase Imaging and Machine Learning
This study developed an in-vitro assessment, the Sickle Erythrocyte Health Index, using quantitative phase imaging (QPI) and machine learning to model the health of RBCs in people with SCD. The Health Index combines assessment of cell deformation, sickle-shaped classification, and membrane flexibility to evaluate erythrocyte health. (Source: Experimental Hematology)
Source: Experimental Hematology - January 19, 2024 Category: Hematology Authors: Yaw O.N. Ansong-Ansongton, Timothy D. Adamson Tags: Article Source Type: research
Metabolic Regulation of Erythrocyte Development and Disorders
Erythropoiesis presents some unique metabolic requirements as hematopoietic stem cells (HSC) undergo successive steps of lineage differentiation, proliferation, and terminal maturation into highly specialized oxygen transporting cells filled with hemoglobin [1, 2]. Approximately 25 trillion circulating red blood cells (RBC) account for ∼84% of all cells in the adult human body. Over two million RBCs are produced every second to replace aged or damaged ones, representing ∼40% of the total body mass turnover [3]. (Source: Experimental Hematology)
Source: Experimental Hematology - January 16, 2024 Category: Hematology Authors: Junhua Lyu, Min Ni, Mitchell J. Weiss, Jian Xu Source Type: research
IFC Editorial Board
(Source: Experimental Hematology)
Source: Experimental Hematology - January 1, 2024 Category: Hematology Source Type: research
Publisher ’s Note: Introducing article numbering to Experimental Hematology
(Source: Experimental Hematology)
Source: Experimental Hematology - January 1, 2024 Category: Hematology Source Type: research
G-CSF –induced hematopoietic stem cell mobilization from the embryonic hematopoietic niche does not require neutrophils and macrophages
Granulocyte colony-stimulating factor (G-CSF) induces the production of mature neutrophils, promotes the proliferation and differentiation of hematopoietic stem and progenitor cells (HSPCs), and facilitates the release of HSPCs from the bone marrow [1,2]. Its capability to release HSPCs has made G-CSF one of the pioneering cytokines used for HSPC mobilization, a process involving the release of HSPCs from the bone marrow to collect them from the peripheral blood during stem cell transplantation. (Source: Experimental Hematology)
Source: Experimental Hematology - December 29, 2023 Category: Hematology Authors: Ji Wook Kim, Evan A. Fedorov, Leonard I. Zon Tags: Regular Submission Source Type: research
G-CSF induced hematopoietic stem cell mobilization from the embryonic hematopoietic niche does not require neutrophils and macrophages
Granulocyte colony-stimulating factor (G-CSF) induces the production of mature neutrophils, promotes the proliferation and differentiation of hematopoietic stem and progenitor cells (HSPCs), and facilitates the release of HSPCs from the bone marrow1,2. Its capability to release HSPCs has made G-CSF one of the pioneering cytokines used for HSPC mobilization, a process involving the release of HSPCs from the bone marrow to collect them from the peripheral blood during stem cell transplantation. To date, G-CSF remains the most widely employed mobilizing agent and can be combined with other drugs or cytokines, such as AMD3100 ...
Source: Experimental Hematology - December 29, 2023 Category: Hematology Authors: Ji Wook Kim, Evan A. Fedorov, Leonard I. Zon Tags: Article Source Type: research
Purging myeloma cell contaminants and simultaneous expansion of peripheral blood-mobilized stem cells
Hematopoietic stem cells (HSCs) are vital tissue stem cells responsible for producing all hematopoietic cells throughout a person's lifetime [1]. HSCs harvested from the body are essential medical resources for hematopoietic stem cell transplantation (HSCT), which can be classified into two types: allogeneic HSCT and autologous HSCT [2]. These procedures are primarily performed for patients with hematological malignancies after chemotherapy. Commonly, peripheral blood-mobilized HSCs (PBSCs) are used for autologous HSCT as the donor cell source for patients with multiple myeloma or malignant lymphoma [3,4], but this poses s...
Source: Experimental Hematology - December 25, 2023 Category: Hematology Authors: Kantaro Ishitsuka, Hidekazu Nishikii, Takaharu Kimura, Ayano Sugiyama-Finnis, Satoshi Yamazaki Tags: Article Source Type: research
Purging myeloma cell contaminants and simultaneous expansion of peripheral blood mobilised stem cells
HSCs are vital tissue stem cells responsible for producing all hematopoietic cells throughout a person's lifetime [1]. HSCs harvested from the body are essential medical resources for HSCT, which can be classified into two types: allogeneic HSCT and autologous HSCT [2]. These procedures are primarily performed for patients with hematological malignancies after chemotherapy. Commonly, PBSCs are used for autologous HSCT as the donor cell source for patients with multiple myeloma or malignant lymphoma [3,4], but this poses several challenges. (Source: Experimental Hematology)
Source: Experimental Hematology - December 25, 2023 Category: Hematology Authors: Kantaro Ishitsuka, Hidekazu Nishikii, Takaharu Kimura, Ayano Sugiyama-Finnis, Satoshi Yamazaki Tags: Article Source Type: research
Nucleic Acid-Induced Inflammation on Hematopoietic Stem Cells
Hematopoietic stem cells (HSCs) represent a multipotent cell population with the inherent ability to differentiate into all blood lineages. HSCs are born during the definitive developmental wave that follows the primitive and pro definitive waves that give rise to erythrocyte and myeloid cells1. First, HSCs differentiate into multipotent progenitor cells (MPPs). MPPs differentiate into common myeloid progenitors (CMPs) and common lymphoid progenitors (CLPs). CMPs give rise to erythrocytes, megakaryocytes, granulocytes, and monocytes/macrophages, while CLPs give rise to B cells, T cells, and natural killer cells2. (Source: ...
Source: Experimental Hematology - December 25, 2023 Category: Hematology Authors: Giang To Vu, Valerie Awad, Maria Feliz Norberto, Teresa V. Bowman, Eirini Trompouki Source Type: research
AKT2 inhibition accelerates the acquisition of phagocytic ability in induced pluripotent stem cell –derived neutrophils
Neutrophils act as the first-line immune players during bacterial infections. Typically, the first leukocytes recruited to inflammatory sites protect humans from pathogens [1]. Despite recent progress in antibacterial and antifungal therapies, the strategy for dealing with the risk of severe infections associated with prolonged neutropenia caused by chemotherapy, radiotherapy, cancer, or hematologic diseases needs to be further explored [2 –4]. Some reports demonstrated that the use of granulocyte transfusions (GTX) prevented life-threatening infections in patients with neutropenia induced by chemotherapy or hematopoieti...
Source: Experimental Hematology - December 13, 2023 Category: Hematology Authors: Toshiya Hino, Fumio Nakahara, Masashi Miyauchi, Yusuke Ito, Yosuke Masamoto, Ken Morita, Yuki Kagoya, Hirotatsu Kojima, Mineo Kurokawa Tags: Brief communication Source Type: research
AKT2 inhibition accelerates the acquisition of phagocytic ability in iPSCs-derived neutrophils
Neutrophils act as the first-line immune players during bacterial infections. Typically, the first leukocytes recruited to inflammatory sites protect humans from pathogens [1]. Despite recent progress in antibacterial and antifungal therapies, the strategy for dealing with risk of severe infections associated with prolonged neutropenia caused by chemotherapy, radiotherapy, cancer, or hematological diseases, needs to be further explored [2 –4]. Some reports demonstrated that the use of granulocyte transfusions (GTX) prevented life-threatening infections in patients with neutropenia induced by chemotherapy or hematopoietic...
Source: Experimental Hematology - December 13, 2023 Category: Hematology Authors: Toshiya Hino, Fumio Nakahara, Masashi Miyauchi, Yusuke Ito, Yosuke Masamoto, Ken Morita, Yuki Kagoya, Hirotatsu Kojima, Mineo Kurokawa Tags: Brief Communication Source Type: research
Ex vivo Hematopoietic Stem Cell Expansion Technologies: Recent Progress, Applications, and Open Questions
Hematopoietic stem cells (HSCs) are a rare but powerful cell type with the role of supporting blood and immune cell production throughout our lives. It is estimated that there are only ∼100,000 active HSCs in adult humans yet they are responsible for generating ∼90% of the cells in our bodies1,2. HSCs can also stably regenerate the entire blood and immune system following transplantation. To achieve these functions, HSCs balance self-renewal to generate more stem cells and dif ferentiation to generate any of the mature blood cell types via a spectrum of intermediate progenitor cells3. (Source: Experimental Hematology)
Source: Experimental Hematology - December 8, 2023 Category: Hematology Authors: Grace A. Meaker, Adam C. Wilkinson Source Type: research
Epigenetic vulnerabilities of leukemia harboring inactivating EZH2 mutations
Epigenetic regulation encompasses the covalent modification of histone proteins, such as acetylation, phosphorylation, and ubiquitylation, resulting in the activation or inhibition of gene transcription and regulation of chromatin structure (1). Dysregulation of histone methylation is associated with various stages of carcinogenesis, including tumor initiation, promotion, and progression. Thus, targeting histone methyltransferases is being explored as a cancer therapeutic approach (2). The polycomb repressive complex 2 (PRC2), which is responsible for the mono-, di- and tri-methylation of histone 3 at lysine 27 promotes th...
Source: Experimental Hematology - December 8, 2023 Category: Hematology Authors: Mona A. Alqazzaz, Genna M. Luciani, Victoria Vu, Raquel Martinez Machado, Magdalena M. Szewczyk, Ella C. Adamson, Sehyun Cheon, Fengling Li, Cheryl H. Arrowsmith, Mark D. Minden, Dalia Barsyte-Lovejoy Tags: Article Source Type: research
