European Journal of Applied Physiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Development and evaluation of a test strip for the rapid detection of antibody against equine infectious anemia virus
In this study, we designed and developed a colloidal gold immunochromatographic (GICG) test strip to detect antibodies against EIAV based on the double-antigen sandwich. Both the p26 and gp45 proteins were used as the capture antigens, which may help to improve the positive detection rate of the strip. We found that the sensitivity of the test strip was 8 to 16 times higher than those of two commercially available ELISA tests and 128 to 256 times higher than AGID, but 8 to 16 times lower than that of western blotting. The strip has good specificity and stability. When serum samples from experimental horses immunized with t...
Source: European Journal of Applied Physiology - January 8, 2024 Category: Physiology Source Type: research

Biosynthesis of eriodictyol in citrus waster by endowing P450BM3 activity of naringenin hydroxylation
This study provided a theoretical guide for the rational design of P450s to the nonnative compounds.Key points•The compound I is proposed as the starting point for the rational design of the P450BM3•“Flying kite model” is proposed based on the distance between O of Cmpd I and C3′/C5′ of naringenin•Mutant M15-5 with 1.6-fold of activity than M13 was obtained by ab initio modificationGraphical abstract (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 8, 2024 Category: Physiology Source Type: research

Single-session measures of quadriceps neuromuscular function are reliable in healthy females and unaffected by age
ConclusionExcellent reliability of voluntary and evoked force andvastus lateralis EMG outcomes measured in healthy females can be attained in one experimental session, irrespective of age. Female neuromuscular function can be accurately assessed across the lifespan with minimal inconvenience, increasing feasibility for future research. The random error should however be considered when quantifying age-related differences in neuromuscular function. (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 8, 2024 Category: Physiology Source Type: research

The effect of a single closed-circuit rebreather decompression dive in extremely cold water to cardiac function
ConclusionA single trimix CCR dive in arctic cold water seemed to debilitate both systolic and diastolic function. Although the changes were subtle, they appeared parallel over several parameters. This indicates a real post-dive deterioration in cardiac function instead of only volume-dependent changes. These changes are without a clinical significance in healthy divers. However, in a population with pre-existing or underlying heart problems, such changes may provoke symptomatic problems during or after the dive. (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 8, 2024 Category: Physiology Source Type: research

Analysis of substrate specificity of cytochrome P450 monooxygenases involved in trichothecene toxin biosynthesis
AbstractTrichothecenes are a structurally diverse family of toxic secondary metabolites produced by certain species of multiple fungal genera. All trichothecene analogs share a core 12,13-epoxytrichothec-9-ene (EPT) structure but differ in presence, absence and types of substituents attached to various positions of EPT. Formation of some of the structural diversity begins early in the biosynthetic pathway such that some producing species have few trichothecene biosynthetic intermediates in common. Cytochrome P450 monooxygenases (P450s) play critical roles in formation of trichothecene structural diversity. Within some spec...
Source: European Journal of Applied Physiology - January 6, 2024 Category: Physiology Source Type: research

Improving glucose oxidase catalysis in Aspergillus niger via Vitreoscilla hemoglobin fusion protein
This study identified GoxA, a specific isoform of Gox in the industrial gluconic acid-producing strain ofAspergillus niger. Various forms of VHb expression inA. niger were tested to improve GoxA ’s catalytic efficiency. Surprisingly, the expression of free VHb, both intracellularly and extracellularly, did not promote gluconic acid production during shake flask fermentation. Then, five fusion proteins were constructed by linking Gox and VHb using various methods. Among these, VHb-GS1-GoxA , where VHb’s C-terminus connected to GoxA’s N-terminus via the flexible linker GS1, demonstrated a significantly higher Kcat/Km v...
Source: European Journal of Applied Physiology - January 6, 2024 Category: Physiology Source Type: research

Poly- β-hydroxybutyrate production by Synechocystis MT_a24 in a raceway pond using urban wastewater
This study aimed to investigate PHB production in the cyanobacteriumSynechocystis sp. PCC6714 MT_a24 in an outdoor bioreactor using urban wastewater as a sole nutrient source. The culture was grown in a thin-layer raceway pond with a working volume of 100 L, reaching a biomass density of up to 3.5  g L−1 of cell dry weight (CDW). The maximum PHB content was found under nutrient-limiting conditions in the late stationary phase, reaching 23.7  ± 2.2% PHB per CDW. These data are one of the highest reported for photosynthetic production of PHB by cyanobacteria, moreover using urban wastewater in pilot-scale cultivation...
Source: European Journal of Applied Physiology - January 6, 2024 Category: Physiology Source Type: research

Bacterial community and filamentous population of industrial wastewater treatment plants in Belgium
AbstractThe discharge of industrial water requires the removal of its pollutants, where biological wastewater treatment plants (WWTPs) are the most used systems. Biological WWTPs make use of activated sludge (AS), where bacteria are responsible for the removal of pollutants. However, our knowledge of the microbial communities of industrial plants is limited. Understanding the microbial population is essential to provide solutions to industrial problems such as bulking. The aim of this study was to identify at a high taxonomic resolution the bacterial population of 29 industrial WWTPs using 16S rRNA amplicon sequencing. Our...
Source: European Journal of Applied Physiology - January 6, 2024 Category: Physiology Source Type: research

The application of bacteria-derived dehydrogenases and oxidases in the synthesis of gold nanoparticles
AbstractIn this work the green synthesis of gold nanoparticles (Au-NPs) using the oxidoreductive enzymesMyriococcum thermophilum cellobiose dehydrogenase (Mt CDH),Glomerella cingulata glucose dehydrogenase (Gc GDH), andAspergillus niger glucose oxidase (An GOX)) as bioreductants was investigated. The influence of reaction conditions on the synthesis of Au-NPs was examined and optimised. The reaction kinetics and the influence of Au ions on the reaction rate were determined. Based on the kinetic study, the mechanism of Au-NP synthesis was proposed. The Au-NPs were characterized by UV –Vis spectroscopy and transmission ele...
Source: European Journal of Applied Physiology - January 6, 2024 Category: Physiology Source Type: research

Metabolic engineering of Shewanella oneidensis to produce glutamate and itaconic acid
AbstractShewanella oneidensis is a gram-negative bacterium known for its unique respiratory capabilities, which allow it to utilize a wide range of electron acceptors, including solid substrates such as electrodes. For a future combination of chemical production and electro-fermentation, the goal of this study was to expand its product spectrum.S. oneidensis was metabolically engineered to optimize its glutamate production and to enable production of itaconic acid. By deleting the glutamate importergltS for a reduced glutamate uptake andpckA/ptA to redirect the carbon flux towards the TCA cycle, a ∆3 mutant was created. ...
Source: European Journal of Applied Physiology - January 6, 2024 Category: Physiology Source Type: research

Recent findings in methanotrophs: genetics, molecular ecology, and biopotential
AbstractThe potential consequences for mankind could be disastrous due to global warming, which arises from an increase in the average temperature on Earth. The elevation in temperature primarily stems from the escalation in the concentration of greenhouse gases (GHG) such as CO2, CH4, and N2O within the atmosphere. Among these gases, methane (CH4) is particularly significant in driving alterations to the worldwide climate. Methanotrophic bacteria possess the distinctive ability to employ methane as both as source of carbon and energy. These bacteria show great potential as exceptional biocatalysts in advancing C1 bioconve...
Source: European Journal of Applied Physiology - January 6, 2024 Category: Physiology Source Type: research

Key factors for the survival of Lactiplantibacillus plantarum IDCC 3501 in manufacturing and storage
AbstractFunctional microbiome development has steadily increased; with this, the viability of microbial strains must be maintained not only after the manufacturing process but also at the time of consumption. Survival is threatened by various unavoidable factors during freeze-drying and shelf storage. Here, the aim was to optimize the manufacturing process of the functional strainLactiplantibacillus plantarum IDCC 3501 after freeze-drying and storage. Explosive growth was achieved using a medium composition with two nitrogen sources and a mineral, and growth was drastically improved by neutralizing the medium pH during the...
Source: European Journal of Applied Physiology - January 5, 2024 Category: Physiology Source Type: research

Development of genetic markers in Yarrowia lipolytica
AbstractThe oleaginous yeastYarrowia lipolytica represents a potential microbial cell factory for the recombinant production of various valuable products. Currently, the commonly used selection markers for transformation inY. lipolytica are limited, and successive genetic manipulations are often restricted by the number of available selection markers. In our study, we developed a dominant marker,dsdA, which encodes a D-serine deaminase for genetic manipulation inY. lipolytica. InY. lipolytica, this marker confers the ability to use D-serine as a nitrogen source. In addition, the selection conditions of several infrequently...
Source: European Journal of Applied Physiology - January 5, 2024 Category: Physiology Source Type: research

The interactions between the host immunity and intestinal microorganisms in fish
AbstractThere is a huge quantity of microorganisms in the gut of fish, which exert pivotal roles in maintaining host intestinal and general health. The fish immunity can sense and shape the intestinal microbiota and maintain the intestinal homeostasis. In the meantime, the intestinal commensal microbes regulate the fish immunity, control the extravagant proliferation of pathogenic microorganisms, and ensure the intestinal health of the host. This review summarizes developments and progress on the known interactions between host immunity and intestinal microorganisms in fish, focusing on the recent advances in zebrafish (Da...
Source: European Journal of Applied Physiology - January 5, 2024 Category: Physiology Source Type: research

Novel Gluconobacter oxydans strains selected from Kombucha with potential postbiotic activity
AbstractGastric and colorectal cancer are among the most frequently diagnosed malignancies of the gastrointestinal tract. Searching for methods of therapy that complements treatment or has a preventive effect is desirable. Bacterial metabolites safe for human health, which have postbiotic effect, are of interest recently. The study aimed to preliminary assessment of the safety, antimicrobial, and anti-cancer activity of cell-free metabolites ofGluconobacter oxydans strains isolated from Kombucha beverages as an example of the potential postbiotic activity of acetic acid bacteria (AAB). The study material consisted of five ...
Source: European Journal of Applied Physiology - January 5, 2024 Category: Physiology Source Type: research