European Journal of Applied Physiology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Cleavage of cell junction proteins as a host invasion strategy in leptospirosis
AbstractInfection and invasion are the prerequisites for developing the disease symptoms in a host. While the probable mechanism of host invasion and pathogenesis is known in many pathogens, very little information is available onLeptospira invasion/pathogenesis. For causing systemic infectionLeptospira must transmigrate across epithelial barriers, which is the most critical and challenging step. Extracellular and membrane-bound proteases play a crucial role in the invasion process. An extensive search for the proteins experimentally proven to be involved in the invasion process through cell junction cleavage in other path...
Source: European Journal of Applied Physiology - January 10, 2024 Category: Physiology Source Type: research

Community composition of phytopathogenic fungi significantly influences ectomycorrhizal fungal communities during subtropical forest succession
In this study, we used high-throughput sequencing to investigate fungal communities in young, intermediate, and old subtropical forests. At the genus level, EMF communities were dominated bySebacina,Russula, andLactarius, whileMycena was the most abundant genus in pathogenic fungal communities. The relative abundances of EMF and phytopathogenic fungi in different stages showed no significant difference with the regulation of different factors. We discovered that interactions between phytopathogenic fungi and EMF maintained a dynamic balance under the influence of the differences in soil quality attributed to each forest su...
Source: European Journal of Applied Physiology - January 10, 2024 Category: Physiology Source Type: research

Protein secretion and associated stress in industrially employed filamentous fungi
AbstractApplication of filamentous fungi for the production of commercial enzymes such as amylase, cellulase, or xylanase is on the rise due to the increasing demand to degrade several complex carbohydrates as raw material for biotechnological processes. Also, protein production by fungi for food and feed gains importance. In any case, the protein production involves both cellular synthesis and secretion outside of the cell. Unfortunately, the secretion of proteins or enzymes can be hampered due to accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) as a result of too high synthesis of enzymes ...
Source: European Journal of Applied Physiology - January 10, 2024 Category: Physiology Source Type: research

Microbiome analysis reveals the intestinal microbiota characteristics and potential impact of Procambarus clarkii
AbstractThe intestinal microbiota interacts with the host and plays an important role in the immune response, digestive physiology, and regulation of body functions. In addition, it is also well documented that the intestinal microbiota of aquatic animals are closely related to their growth rate. However, whether it resulted in different sizes of crayfish in the rice-crayfish coculture model remained vague. Here, we analyzed the intestinal microbiota characteristics of crayfish of three sizes in the same typical rice-crayfish coculture field by high-throughput sequencing technology combined with quantitative real-time poly...
Source: European Journal of Applied Physiology - January 10, 2024 Category: Physiology Source Type: research

Reliability of recovery heart rate variability measurements as part of the Lamberts Submaximal Cycle Test and the relationship with training status in trained to elite cyclists
ConclusionsLnRMSSD60 s can be measured reliably after the LSCT and can predict PPO,VO2peak and 40  km TT performance well in trained-to-elite cyclists. These findings suggest that recovery LnRMSSD can potentially play an important role in monitoring and fine-tuning training prescriptions in trained-to-elite cyclists. (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 10, 2024 Category: Physiology Source Type: research

Downhill running increases markers of muscle damage and impairs the maximal voluntary force production as well as the late phase of the rate of voluntary force development
ConclusionDownhill running affected the knee extensors RVFD late but not early phase. The RVFD late phase may be used as an additional marker of muscle damage in trail running. (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 10, 2024 Category: Physiology Source Type: research

Engineering the Tat-secretion pathway of Bacillus licheniformis for the secretion of cytoplasmic enzyme arginase
AbstractThe industrial bacteriumBacillus licheniformis has long been used as a microbial factory for the production of enzymes due to its ability to secrete copious amounts of native extracellular proteins and its generally regarded as safe (GRAS) status. However, most attempts to useB. licheniformis to produce heterologous and cytoplasmic enzymes primarily via the general secretory (Sec) pathway have had limited success. The twin-arginine transport (Tat) pathway offers a promising alternative for the extracellular export of Sec-incompatible proteins because it transports full, correctly folded proteins. However, compared ...
Source: European Journal of Applied Physiology - January 9, 2024 Category: Physiology Source Type: research

A novel conserved B-cell epitope in pB602L of African swine fever virus
This study found one novel conserved B-cell epitope474SKENLTPDE482.•In the 3D structure,474SKENLTPDE482is exposed on the surface of ASFV pB602L, forming a curved linear structure. (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 9, 2024 Category: Physiology Source Type: research

New bacteriophage-derived lysins, LysJ and LysF, with the potential to control Bacillus anthracis
AbstractBacillus anthracis is an etiological agent of anthrax, a severe zoonotic disease that can be transmitted to people and cause high mortalities. Bacteriophages and their lytic enzymes, endolysins, have potential therapeutic value in treating infections caused by this bacterium as alternatives or complements to antibiotic therapy. They can also be used to identify and detectB. anthracis. Endolysins of twoB. anthracis Wbetavirus phages, J5a and F16Ba which were described by us recently, differ significantly from the best-knownB. anthracis phage endolysin PlyG fromWbetavirus genus bacteriophage Gamma and a few otherWbet...
Source: European Journal of Applied Physiology - January 9, 2024 Category: Physiology Source Type: research

Expression of virus-like particles (VLPs) of foot-and-mouth disease virus (FMDV) using Saccharomyces cerevisiae
AbstractWe engineeredSaccharomyces cerevisiae to express structural proteins of foot-and-mouth disease virus (FMDV) and produce virus-like particles (VLPs). The gene, which encodes four structural capsid proteins (VP0 (VP4 and VP2), VP3, and VP1), followed by a translational “ribosomal skipping” sequence consisting of 2A and protease 3C, was codon-optimized and chemically synthesized. The cloned gene was used to transformS. cerevisiae 2805 strain. Western blot analysis revealed that the polyprotein consisting of VP0, VP3, and VP1 was processed into the discrete capsid proteins. Western blot analysis of 3C confirmed the...
Source: European Journal of Applied Physiology - January 9, 2024 Category: Physiology Source Type: research

Acute effects of caffeine or quercetin ingestion on motor unit firing pattern before and after resistance exercise
AbstractThe aim of the present study was to investigate the acute effect of caffeine or quercetin ingestion on motor unit firing patterns and muscle contractile properties before and after resistance exercise. High-density surface electromyography (HDs-EMG) during submaximal contractions and electrically elicited torque in knee extensor muscles were measured before (PRE) and 60  min after (POST1) ingestion of caffeine, quercetin glycosides, or placebo, and after resistance exercise (POST2) in ten young males. The Convolution Kernel Compensation technique was used to identify individual motor units of the vastus lateralis ...
Source: European Journal of Applied Physiology - January 9, 2024 Category: Physiology Source Type: research

Association between physical fitness tests and neuromuscular properties
ConclusionGenerally developed physical fitness tests were mainly explained by morphological factors, but were weakly affected by neural factors involved in performance. (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 9, 2024 Category: Physiology Source Type: research

Added insult to injury? The response of meat-associated pathogens to proposed antimicrobial interventions
This study provides science-based evidence of the efficacy of multi-hurdle antimicrobial system using green-label marinades and PFW or UV to inactivate pathogens without upregulating virulence traits in surviving cells. This study will inform policymakers and food industry stakeholders and reinforces the need to incorporate in-line novel technologies to ensure consumer safety.Key points•Salmonella and C. jejuni showed increased cell activity in immediate response to stress.•Virulence genes showed increased expression when treated with natural antimicrobials and sPFW.•Reduced immediate transcriptomic response to gPFW ...
Source: European Journal of Applied Physiology - January 8, 2024 Category: Physiology Source Type: research

Development and evaluation of a test strip for the rapid detection of antibody against equine infectious anemia virus
In this study, we designed and developed a colloidal gold immunochromatographic (GICG) test strip to detect antibodies against EIAV based on the double-antigen sandwich. Both the p26 and gp45 proteins were used as the capture antigens, which may help to improve the positive detection rate of the strip. We found that the sensitivity of the test strip was 8 to 16 times higher than those of two commercially available ELISA tests and 128 to 256 times higher than AGID, but 8 to 16 times lower than that of western blotting. The strip has good specificity and stability. When serum samples from experimental horses immunized with t...
Source: European Journal of Applied Physiology - January 8, 2024 Category: Physiology Source Type: research

Biosynthesis of eriodictyol in citrus waster by endowing P450BM3 activity of naringenin hydroxylation
This study provided a theoretical guide for the rational design of P450s to the nonnative compounds.Key points•The compound I is proposed as the starting point for the rational design of the P450BM3•“Flying kite model” is proposed based on the distance between O of Cmpd I and C3′/C5′ of naringenin•Mutant M15-5 with 1.6-fold of activity than M13 was obtained by ab initio modificationGraphical abstract (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - January 8, 2024 Category: Physiology Source Type: research