Aspergillus niger production of pectinase and α-galactosidase for enzymatic soy processing
Publication date: Available online 17 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Qian Li, Christopher S. Ray, Nicholas V. Callow, Abdullah Al Loman, S.M. Mahfuzul Islam, Lu-Kwang JuAbstractSoybean is a most promising sustainable protein source for feed and food to help meet the protein demand of the rapidly rising global population. To enrich soy protein, the environment-friendly enzymatic processing requires multiple carbohydrases including cellulase, xylanase, pectinase, α-galactosidase and sucrase. Besides enriched protein, the processing adds value by generating monosaccharides that are ready feeds...
Source: Enzyme and Microbial Technology - November 18, 2019 Category: Biotechnology Source Type: research

Directed evolution of RhlI to generate new and increased quorum sensing signal molecule catalytic activities
Publication date: Available online 16 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Biao Zhang, Lili Ren, Dayong Xu, Haonan Wang, Zhuo Chen, Bingrong Zhang, Xin Zeng, Lianhong Sun, Feng LiAbstractQuorum sensing is a population density-dependent gene expression regulation mechanism in bacteria. The substrate specificity of RhlI, an enzyme in the RhlI–RhlR quorum sensing system of Pseudomonas aeruginosa, was explored by directed evolution to gain insight into the molecular mechanisms of quorum sensing. RhlI catalyzes S-adenosyl methionine and butanoyl or hexanoyl acyl carrier protein to form N-butanoyl hom...
Source: Enzyme and Microbial Technology - November 16, 2019 Category: Biotechnology Source Type: research

Bio-assay of Acintobacter baumannii using DNA conjugated with gold nano-star: A new platform for microorganism analysis
In this study, gold nano-star was synthesized and used for bio-conjugation with pDNA toward the detection of target sequences. Synthesized probe (5ʹ TTG TGA ACT ATT TAC GTC AGC ATG C3ʹ) of Acinetobacter baumannii was found with excellent sensitivity. After the hybridization of pDNA with cDNA, target DNA (5ʹ GCA TGC TGA CGT AAA TAG TTC ACA A 3ʹ) was easily measured. According to ultra-sensitivity of the engineered optical DNA-based bio-assay, it is potentially applied in the bacterial detection of the environmental and clinical specimens. Here, the selection of engineered biosensor in the presence of two mismatch sequen...
Source: Enzyme and Microbial Technology - November 13, 2019 Category: Biotechnology Source Type: research

Cloning and expression of the Bacillus amyloliquefaciens transglutaminase gene in E. coli using a bicistronic vector construction
Publication date: Available online 12 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Lovaine Silva Duarte, Laísa Quadros Barsé, Pedro Ferrari Dalberto, William Tadeu Santos da Silva, Rafael Costa Rodrigues, Pablo Machado, Luiz Augusto Basso, Cristiano Valim Bizarro, Marco Antônio Záchia AyubAbstractTransglutaminases (TGases) are a class of transferases widely used in the food and biotechnology industries. In this work, we describe the production of recombinant Bacillus amyloliquefaciens TGase in Escherichia coli, obtaining the protein in its soluble and active form. In order to reduce TGase activity ins...
Source: Enzyme and Microbial Technology - November 13, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: January 2020Source: Enzyme and Microbial Technology, Volume 132Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - November 13, 2019 Category: Biotechnology Source Type: research