A novel green approach for fabricating visible, light sensitive nano-broccoli-like antimony trisulfide by marine Sb(V)-reducing bacteria: revealing potential self-purification in coastal zones
Publication date: Available online 20 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Haikun Zhang, Jingyi Xie, Yanyu Sun, Ailing Zheng, Xiaoke HuAbstractAntimony trisulfide (Sb2S3) is industrially important for processes ranging from a semiconductor dopant through batteries to a flame retardant. Approaches for fabricating Sb2S3 nanostructures or thin films are by chemical or physicochemical methods, while there have been no report focused on the biological synthesis of nano Sb2S3. In the present study, we fabricated nano-broccoli-like Sb2S3 using Sb(V) reducing bacteria. Thirty four marine and terrestrial st...
Source: Enzyme and Microbial Technology - January 21, 2020 Category: Biotechnology Source Type: research
Taguchi Design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies
This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT). The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT. In addition, amide peaks at 3478 and 1640 cm-1 in the fourier transform infrared spectra affirmed that CRL was covalently bonded to ALG/NC/MMT. The optimized Taguchi Design-assist...
Source: Enzyme and Microbial Technology - January 20, 2020 Category: Biotechnology Source Type: research
High level and enantioselective production of L-phenylglycine from racemic mandelic acid by engineered Escherichia coli using response surface methodology
Publication date: Available online 18 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Cun-Duo Tang, Hong-Ling Shi, Yuan-Yuan Jia, Xiang Li, Lin-Feng Wang, Jian-He Xu, Lun-Guang Yao, Yun-Chao KanAbstractL-Phenylglycine (L-PHG) is a member of unnatural amino acids, and becoming more and more important as intermediate for pharmaceuticals, food additives and agrochemicals. However, the existing synthetic methods for L-PHG mainly rely on toxic cyanide chemistry and multistep processes. To provide green, safe and high enantioselective alternatives, we envisaged cascade biocatalysis for the one-pot synthesis of L-PH...
Source: Enzyme and Microbial Technology - January 20, 2020 Category: Biotechnology Source Type: research
Saturation mutagenesis to improve the degradation of azo dyes by versatile peroxidase and application in form of VP-coated yeast cell walls
Publication date: Available online 15 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Karla Ilić Đurđić, Raluca Ostafe, Aleksandra Đurđević Đelmaš, Nikolina Popović, Stefan Schillberg, Rainer Fischer, Radivoje ProdanovićAbstractAzo dyes are toxic and carcinogenic synthetic pigments that accumulate as pollutants in aquatic bodies near textile factories. The pigments are structurally diverse, and bioremediation is mostly limited to single dye compounds or related groups. Versatile peroxidase (VP) from Pleurotus eryngii is a heme-containing peroxidase with a broad substrate spectrum that can break dow...
Source: Enzyme and Microbial Technology - January 15, 2020 Category: Biotechnology Source Type: research
Characterization of a recombinant L-ribose isomerase from Mycetocola miduiensis and its application for the production of L-ribulose
Publication date: Available online 13 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Shahid Mahmood, Muhammad Waheed Iqbal, Tahreem Riaz, Hinawi A.M. Hassanin, Yingying Zhu, Dawei Ni, Wanmeng MuAbstractAn enzyme, L-ribose isomerase (L-RI), mostly catalyzes the isomerization of L-ribose and L-ribulose. These so-called rare sugars are essential for the treatment of cancer and other viral diseases. In the present study, L-ribose isomerase produced from a bacterium, Mycetocola miduiensis (Mm-LRIse), by using L-ribose as a carbon source. The recombinant L-ribose isomerase gene was cloned and overexpressed from M....
Source: Enzyme and Microbial Technology - January 15, 2020 Category: Biotechnology Source Type: research
Functional expression of recombinant hybrid enzymes composed of bacterial and insect’s chitinase domains in E. coli
Publication date: Available online 11 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Aron Paek, Min Jae Kim, Hee Yun Park, Je Geun Yoo, Seong Eun JeongAbstractTo elucidate the functional alteration of the recombinant hybrid chitinases composed of bacterial and insect’s domains, we cloned the constitutional domains from chitinase-encoding cDNAs of a bacterial species, Bacillus thuringiensis (BtChi) and a lepidopteran insect species, Mamestra brassicae (MbChi), respectively, swapped one’s leading signal peptide (LSP) - catalytic domain (CD) - linker region (LR) (LCL) with the other’s chitin binding domai...
Source: Enzyme and Microbial Technology - January 12, 2020 Category: Biotechnology Source Type: research
Elucidation of the decolorization of Congo Red by Trametes versicolor laccase in presence of ABTS through cyclic voltammetry
Publication date: Available online 2 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Rituparna Saha, Mainak MukhopadhyayAbstractThe azo dye Congo red is heavily used in textile industries and is actively present in the wastewater run-offs. Its structural complexity and physical characteristics make it resistant to the physicochemical treatments employed by the industry. Over time, application of the enzyme laccase has proved to be quite useful due to its ability to oxidize and eventually decolorize the dye. Moreover, the use of ABTS as the electron mediator also helps in enhancing the oxidizing capability of ...
Source: Enzyme and Microbial Technology - January 3, 2020 Category: Biotechnology Source Type: research
Heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum for luteolin glucoside production
Publication date: Available online 27 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Young-Wook Chin, Se-Won Jang, Hee-Soon Shin, Tae-Wan Kim, Sun-Ki Kim, Cheon-Seok Park, Dong-Ho SeoAbstractAmylosucrase (ASase) has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization. In the present study, the properties of Deinococcus geothermalis ASase (DGAS) expressed in Corynebacterium glutamicum (cDGAS) and purified via Ni-NTA affinity chromatography were compared to those of DGAS expressed in Escherichia coli (eDGAS). The pH profile of cDG...
Source: Enzyme and Microbial Technology - December 29, 2019 Category: Biotechnology Source Type: research
Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production
In this study, recombinant E. coli (rE. coli) was immobilized on untreated and treated multiwalled carbon nanotubes (MWCNTs) for xylitol production. The immobilized rE. coli on untreated MWCNTs gave the highest xylitol production (5.47 g L-1) and a productivity of 0.22 g L-1 h-1. The doubling time for the immobilized cells increased up to 20.40 h and was higher than that of free cells (3.67 h). Cell lysis of the immobilized cells was reduced by up to 73 %, and plasmid stability improved by up to 17 % compared to those of free cells. Xylitol production using the optimum parameters (pH 7.4, 0.005 mM and 29 ...
Source: Enzyme and Microbial Technology - December 24, 2019 Category: Biotechnology Source Type: research
Bio-transformation of green tea infusion with tannase and its improvement on adipocyte metabolism
Publication date: April 2020Source: Enzyme and Microbial Technology, Volume 135Author(s): Hae-Soo KIM, Do Yeon JEON, Hafiz Muhammad Ahmad JAVAID, Namood E. SAHAR, Ha-Nul LEE, Seong-Jin HONG, Joo Young HUH, Young-Min KIMAbstractCatechins in green tea possess various health benefits. Enzymatic treatment improves physiological activities by inducing bioconversion of catechins. Here, we investigated the effect of green tea infusion (GT) after tannase treatment, which transforms (-)-epigallocatechin gallate (EGCG) to gallic acid (GA) and (-)-epigallocatechin (EGC), on adipocyte differentiation and mature adipocyte metabolism. T...
Source: Enzyme and Microbial Technology - December 24, 2019 Category: Biotechnology Source Type: research
Zn2+ stapling of N and C-terminal maintains stability and substrate affinity in GH26 endo-mannanase
Publication date: Available online 20 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Gaurav Singh Kaira, Dandamudi Usharani, Mukesh KapoorAbstractMetal binding sites are present in one-third of proteins and are crucial for biological functions and structural maintenance. GH26 endo-mannanase (ManB-1601) from Bacillus sp. harbors a Zn2+ binding site which connects N (H1, H23) and C (E336)-terminal residues. Present study reveals how native circularization of ManB-1601 through Zn2+ coordination regulates the structure-function. We generated individual Zn2+ coordinating mutants and characterized them using bioc...
Source: Enzyme and Microbial Technology - December 21, 2019 Category: Biotechnology Source Type: research
Construction of Arming Yarrowia lipolytica Surface-displaying Soybean Seed Coat Peroxidase for Use as Whole-cell Biocatalyst
This study also provides a useful strategy for heterologous expression of proteins which could be toxic to intracellular content of host cells. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 21, 2019 Category: Biotechnology Source Type: research
Expression and purification of the full-length N-acetylgalactosaminyltransferase and galactosyltransferase from Campylobacter jejuni in Escherichia coli
In this study, the full-length CgtA43456 (β-(1→4)-N-acetylgalactosaminyltransferase) and CgtB11168 (β-(1→3)-galactosyltransferase) were successfully produced from Escherichia coli through the optimization of E. coli–preferable codon usage, selection of E. coli strain, and use of the molecular chaperone GroEL-GroES (GroEL/ES). The CgtA43456 enzyme was produced as a soluble form in E. coli C41(DE3) co-expressed with codon-optimized CgtA43456 and GroEL/ES. However, soluble CgtB11168 was well expressed in E. coli C41(DE3) with only the codon-optimized CgtB11168. Rather, when co-expressed with GroEL/ES, total production...
Source: Enzyme and Microbial Technology - December 14, 2019 Category: Biotechnology Source Type: research
Efficient synthesis of L-phosphinothricin using a novel aminoacylase mined from Stenotrophomonas maltophilia
This study provides a potential route for the industrial production of L-PPT.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 14, 2019 Category: Biotechnology Source Type: research
High-level expression of Thermobifida fusca glucose isomerase for high fructose corn syrup biosynthesis
In this study, an efficient expression system for a Thermobifida fusca GIase (GIaseTfus) in Escherichia coli was firstly designed via a two-stage feeding strategy for improving expression level. The cultivation strategy was performed at an exponential feeding rate during the pre-induction phase, followed by a gradient-decreasing feeding rate at the induction phase in a 3-L fermenter. During this process, the effect of induction conditions and the complex nitrogen supplementation in feeding solutions on GIaseTfus production were investigated and optimized. Under the optimal conditions, the yield of GIaseTfus reached 124.1 U...
Source: Enzyme and Microbial Technology - December 14, 2019 Category: Biotechnology Source Type: research
