Metabolic Engineering of Pichia pastoris for Production of Isopentanol (3-Methyl-1-butanol)
Publication date: Available online 4 April 2020Source: Enzyme and Microbial TechnologyAuthor(s): Wiparat Siripong, Clara Angela, Sutipa Tanapongpipat, Weerawat Runguphan (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - April 5, 2020 Category: Biotechnology Source Type: research

Engineered P450 BM3 and cpADH5 coupled cascade reaction for β-oxo fatty acid methyl ester production in whole cells
Publication date: Available online 3 April 2020Source: Enzyme and Microbial TechnologyAuthor(s): Yunus Ensari, Gustavo de Almeida Santos, Anna Joëlle Ruff, Ulrich Schwaneberg (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - April 4, 2020 Category: Biotechnology Source Type: research

High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris
Publication date: Available online 1 April 2020Source: Enzyme and Microbial TechnologyAuthor(s): Yankun Yang, Guoqiang Liu, Xiao Chen, Meng Liu, Chunjun Zhan, Xiuxia Liu, Zhonghu Bai (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - April 2, 2020 Category: Biotechnology Source Type: research

A novel, enantioselective, thermostable recombinant hydantoinase to aid the synthesis of industrially valuable non-proteinogenic amino acids
Publication date: Available online 19 March 2020Source: Enzyme and Microbial TechnologyAuthor(s): Anupama S. Engineer, Kunal K. Yadav, Pranav R. Kshirsagar, Prashant K. Dhakephalkar (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 20, 2020 Category: Biotechnology Source Type: research

Characterization of a recombinant D-mannose-producing D-lyxose isomerase from Caldanaerobius polysaccharolyticus
Publication date: August 2020Source: Enzyme and Microbial Technology, Volume 138Author(s): Hao Wu, Ming Chen, Cuie Guang, Wenli Zhang, Wanmeng Mu (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 20, 2020 Category: Biotechnology Source Type: research

Efficient keratinase expression via promoter engineering strategies for degradation of feather wastes
Publication date: June 2020Source: Enzyme and Microbial Technology, Volume 137Author(s): Jin-Song Gong, Jin-Peng Ye, Li-Yan Tao, Chang Su, Jiufu Qin, Yan-Yan Zhang, Heng Li, Hui Li, Zheng-Hong Xu, Jin-Song Shi (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 20, 2020 Category: Biotechnology Source Type: research

Significance of a family-6 carbohydrate-binding module in a modular feruloyl esterase for removing ferulic acid from insoluble wheat arabinoxylan
Publication date: Available online 12 March 2020Source: Enzyme and Microbial TechnologyAuthor(s): Ai Mamiya, Makiko Sakka, Akihiko Kosugi, Hirotaka Katsuzaki, Akiyoshi Tanaka, Emi Kunitake, Tetsuya Kimura, Kazuo Sakka (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 13, 2020 Category: Biotechnology Source Type: research

High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris
Publication date: Available online 10 March 2020Source: Enzyme and Microbial TechnologyAuthor(s): Zeynep Efsun Duman, Bedri Burak Duraksoy, Fatih Aktaş, John M. woodley, Barış Binay (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 11, 2020 Category: Biotechnology Source Type: research

Engineering the heparin-binding pocket to enhance the catalytic efficiency of a thermostable heparinase III from Bacteroides thetaiotaomicron
Publication date: Available online 9 March 2020Source: Enzyme and Microbial TechnologyAuthor(s): Hao Wang, Lin Zhang, Yang Wang, Jianghua Li, Guocheng Du, Zhen Kang (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 11, 2020 Category: Biotechnology Source Type: research

Engineering of l-threonine aldolase for the preparation of 4-(methylsulfonyl)phenylserine, an important intermediate for the synthesis of florfenicol and thiamphenicol
Publication date: Available online 9 March 2020Source: Enzyme and Microbial TechnologyAuthor(s): Zhicheng Liu, Xi Chen, Qijia Chen, Jinhui Feng, Min Wang, Qiaqing Wu, Dunming Zhu (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 11, 2020 Category: Biotechnology Source Type: research

Bacterial production of maize and human serine racemases as partially active inclusion bodies for D-serine synthesis
Publication date: Available online 4 March 2020Source: Enzyme and Microbial TechnologyAuthor(s): Ruyue Wang, Jinfeng Li, Dongya Dang, Jiong Hu, Yafang Hu, Jun Fan (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 5, 2020 Category: Biotechnology Source Type: research

Catalytic activity and stabilization of phenyl-modified glucose oxidase at high hydrostatic pressure
Publication date: Available online 3 March 2020Source: Enzyme and Microbial TechnologyAuthor(s): Ali Halalipour, Michael R. Duff, Elizabeth E. Howell, José I. Reyes-De-Corcuera (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 5, 2020 Category: Biotechnology Source Type: research

L-amino acid ligase: A promising alternative for the biosynthesis of L-dipeptides
Publication date: Available online 26 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Tao Wang, Yu-fei Zhang, Li-xiao Ning, Yi-fan Wang, Xiao-huan Liu, Rui Li, Xiang-e Chen (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - February 27, 2020 Category: Biotechnology Source Type: research

N-acylation of L-amino acids in aqueous media: evaluation of the catalytic performances of Streptomyces ambofaciens aminoacylases
Publication date: Available online 24 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Mohamed Chafik Bourkaib, Stephane Delaunay, Xavier Framboisier, Hôtel Laurence, Aigle Bertrand, Catherine Humeau, Yann Guiavarc’h, Isabelle Chevalot (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - February 26, 2020 Category: Biotechnology Source Type: research

Exploration of dipeptidyl-peptidase IV (DPP IV) inhibitors in a low-molecular mass extract of the earthworm Eisenia fetida and identification of the inhibitors as amino acids like methionine, leucine, histidine, and isoleucine
Publication date: Available online 20 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Katsuhiro Yoshii, Masako Ogasawara, Jun Wada, Yoshihiro Yamamoto, Kuniyo InouyeAbstractWe have reported previously that the water extract of the earthworm Eisenia fetida has inhibitory effect on human dipeptidyl-peptidase IV (DPP IV) in vitro. Here we studied to identify DPP IV inhibitors in a low-molecular mass extract (designated U3EE) under 3 kDa prepared from the water extract. U3EE showed 50% inhibition (IC50) at the concentration of 5.3 ± 0.3 mg/mL. An inhibitory active fraction obtained by solid-phase extrac...
Source: Enzyme and Microbial Technology - February 21, 2020 Category: Biotechnology Source Type: research