Human Neuronal Cell Based Assay: A New In Vitro Model For Toxicity Evaluation Of Ciguatoxin
Publication date: Available online 14 April 2017 Source:Environmental Toxicology and Pharmacology Author(s): Teresa Coccini, Francesca Caloni, Uliana De Simone Ciguatoxins (CTXs) are emerging marine neurotoxins representing the main cause of ciguatera fish poisoning, an intoxication syndrome which configures a health emergency and constitutes an evolving issue constantly changing due to new vectors and derivatives of CTXs, as well as their presence in new non-endemic areas. The study applied the neuroblastoma cell model of human origin (SH-SY5Y) to evaluate species-specific mechanistic information on CTX toxicity. Metabol...
Source: Environmental Toxicology and Pharmacology - April 14, 2017 Category: Environmental Health Source Type: research

Toxicological effects of phenol on four marine microalgae
Publication date: Available online 13 April 2017 Source:Environmental Toxicology and Pharmacology Author(s): Weiyan Duan, Fanping Meng, Yufei Lin, Guoshan Wang The toxic effects of phenol on four marine microalgae (Dunaliella salina, Platymonas subcordiformis, Phaeodactylum tricornutum Bohlin, and Skeletonema costatum) were evaluated. The 96h EC50 values were 72.29, 92.97, 27.32, and 27.32mgL−1, respectively, which were lower than those values of freshwater microalgae reported in the literature. During a 96-h exposure to a sub-lethal concentration of phenol (1/2 96h EC50) with green alga (D. salina) and diatom (S. c...
Source: Environmental Toxicology and Pharmacology - April 13, 2017 Category: Environmental Health Source Type: research

Chronic Early Childhood Exposure to Arsenic is Associated with a TNF-mediated Proteomic Signaling Response
This study represents the first serum proteomics assessment in children exposed to iAs. Graphical abstract (Source: Environmental Toxicology and Pharmacology)
Source: Environmental Toxicology and Pharmacology - April 10, 2017 Category: Environmental Health Source Type: research

Proinflammatory effects of diesel exhaust particles from moderate blend concentrations of 1st and 2nd generation biodiesel in BEAS-2B bronchial epithelial cells − The FuelHealth project
Publication date: Available online 5 April 2017 Source:Environmental Toxicology and Pharmacology Author(s): Tonje S. Skuland, Magne Refsnes, Pål Magnusson, Michał Oczkowski, Joanna Gromadzka-Ostrowska, Marcin Kruszewski, Remigiusz Mruk, Oddvar Myhre, Anna Lankoff, Johan Øvrevik Biodiesel fuel fuels are introduced at an increasing extent as a more carbon-neutral alternative to reduce CO2-emissions, compared to conventional diesel fuel. In the present study we have investigated the impact of increasing the use of 1st generation fatty acid methyl ester (FAME) biodiesel from current 7% blend (B7) to 20% blend (B...
Source: Environmental Toxicology and Pharmacology - April 5, 2017 Category: Environmental Health Source Type: research

Content decline of SERCA inhibitors saikosaponin a and d attenuates cardiotoxicity and hepatotoxicity of vinegar-baked Radix bupleuri
Publication date: Available online 4 April 2017 Source:Environmental Toxicology and Pharmacology Author(s): Shifeng Wang, Yuxin Zhang, Qiao Zhang, Sha Peng, Chen Shen, Yangyang Yu, Minyu Zhang, Wei Yang, Qinghua Wu, Yanling Zhang, Shiyou Li, Yanjiang Qiao Improper usage of unprocessed Radix bupleuri (chaihu) may cause fetal toxicity. Baking herb with vinegar is believed to attenuate the toxicity. However, the chemical and molecular basis remained unclear. To this end, we investigated the in vitro toxicity of saikosaponin a, c, d, and their hydrolysates saikosaponin b1, b2. Results showed that SSa and SSd possessed higher ...
Source: Environmental Toxicology and Pharmacology - April 4, 2017 Category: Environmental Health Source Type: research

Silica nanoparticles and lead acetate co-exposure triggered synergistic cytotoxicity in A549 cells through potentiation of mitochondria-dependent apoptosis induction
Publication date: Available online 3 April 2017 Source:Environmental Toxicology and Pharmacology Author(s): Chun-Feng Lu, Li-Zhong Li, Wei Zhou, Jun Zhao, Yi-Mei Wang, Shuang-Qing Peng The adverse effects of PM2.5 are the results of combined toxicities of finer particles and their adsorbed toxic pollutants. Nevertheless, the combined toxicity of finer particles and air pollutants still remains unclear. The present study was therefore undertaken to investigate the combined cytotoxicity of silica nanoparticles (nano-SiO2, a typical atmospheric ultrafine particle) and lead acetate (Pb, a representative air pollutant) in A549...
Source: Environmental Toxicology and Pharmacology - April 3, 2017 Category: Environmental Health Source Type: research

Coenzyme Q10 Protects Against Statin-Induced Myotoxicity in Zebrafish Larvae (Danio rerio)
Publication date: Available online 1 April 2017 Source:Environmental Toxicology and Pharmacology Author(s): Rand Pasha, Thomas W. Moon 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR) is the rate-limiting enzyme of the mevalonic acid pathway and is required for cholesterol biosynthesis and the synthesis of Coenzyme Q10 (CoQ10). Statins inhibit HMGCR, thus inhibiting the downstream products of this pathway including the biosynthesis of decaprenyl-pyrophosphate that is critical for the synthesis of Coenzyme Q10 (CoQ10). We show that zebrafish (Danio rerio) larvae treated in tank water with Atorvastatin (ATV; Lipitor) exhibi...
Source: Environmental Toxicology and Pharmacology - April 1, 2017 Category: Environmental Health Source Type: research

Molecular cloning, characterization and expression analysis of metallothionein in the liver of the teleost Acrossocheilus fasciatus exposed to cadmium chloride
Publication date: Available online 29 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Guo-Di Liu, Zhang Sheng, Cong-Cong Hou, Jie Ni, Ying-Li Han, You-Fa Wang, Yang Zhou, Su-Yan Fu, Jun-Quan Zhu Metallothionein (MT) has a characteristic molecular structure with a cysteine-rich content. This unique structure provides metal-binding and redox capabilities and promoting metal homeostasis and detoxification in living animals. In order to evaluate the effects of cadmium (Cd) on hepatic MT expression in the liver of Acrossocheilus fasciatus, we obtained the complete cDNA of the A. fasciatus liver MT for th...
Source: Environmental Toxicology and Pharmacology - March 29, 2017 Category: Environmental Health Source Type: research

Dexmedetomidine attenuates propofol-induce neuroapoptosis partly via the activation of the PI3k/Akt/GSK3 β pathway in the hippocampus of neonatal rats
Publication date: Available online 28 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Jing Lv, Yi Wei, Yehua Chen, Xu Zhang, Zheng Gong, Yage Jiang, Qin Gong, Lifang Zhou, Hao Wang, Yubo Xie Recent studies have demonstrated that propofol causes neurodegeneration in developing brains. Evidence has shown that dexmedetomidine has neuroprotective effects. However, whether dexmedetomidine can reduce propofol-induced neuroapoptosis and by what mechanisms it acts remain unclear. We investigated whether dexmedetomidine can attenuate propofol-induced neuroapoptosis by disturbing the PI3K/Akt/GSK3β pathw...
Source: Environmental Toxicology and Pharmacology - March 28, 2017 Category: Environmental Health Source Type: research

Pharmacokinetics of sulfamethoxazole and trimethoprim in Pacific white shrimp, Litopenaeus vannamei, after oral administration of single-dose and multiple-dose
Publication date: Available online 28 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Rongrong Ma, Yuan Wang, Xiong Zou, Kun Hu, Beibei Sun, Wenhong Fang, Guihong Fu, Xianle Yang The tissue distribution and depletion of sulfamethoxazole (SMZ) and trimethoprim (TMP) were studied in Pacific white shrimp, Litopenaeus vannamei, after single-dose and multiple-dose oral administration of SMZ-TMP (5:1) via medicated feed. In single-dose oral administration, shrimps were fed once at a dose of 100 mg/kg (drug weight/body weight). In multiple-dose oral administration, shrimps were fed three times a day...
Source: Environmental Toxicology and Pharmacology - March 28, 2017 Category: Environmental Health Source Type: research

Occurrence and risk assessment of estrogenic compounds in the East Lake, China
Publication date: Available online 27 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Jun Wang, Yindi Zhu Forty two surface water samples were collected in May and June of 2013 in five lakelets of the East Lake, China. Four steroid hormones (17β-estradiol (βE2), estrone (E1), 17α-estradiol (αE2) and 17α-ethinylestradiol (αEE2)) were analyzed in these samples. Determination of four estrogenic compounds was performed on high performance liquid chromatography/mass spectrometry (HPLC/MS). βE2 was detected with the highest detection frequency of 62% and its concentr...
Source: Environmental Toxicology and Pharmacology - March 27, 2017 Category: Environmental Health Source Type: research

Endo-symbiont mediated synthesis of gold nanobactericides and their activity against human pathogenic bacteria
Publication date: Available online 27 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Baker Syed, M.N. Nagendra Prasad, K. Mohan Kumar, B.L. Dhananjaya, Sreedharamurthy Satish Synthesis of gold nanobactericides (AuNBs) were achieved by treating 1mM chloroaurate with cell free supernatant of Aneurinibacillus migulanus. Formation of AuNBs was initially was monitored with change in color to ruby red. Further confirmation was assessed with UV-Visible spectra with maximum absorption occurring at 510nm. Transmission electron microscopy (TEM) analysis revealed the polydispersity of AuNBs with size distribu...
Source: Environmental Toxicology and Pharmacology - March 27, 2017 Category: Environmental Health Source Type: research

PM2.5-induced alterations of cell cycle associated gene expression in lung cancer cells and rat lung tissues
In conclusion, PM2.5 can induce significantly alterations of p53 and CDK2 in the early phase, Cdc2 and cyclin B in mid-term and p21 in long-term exposure. The degree of PM2.5-induced damage to the trachea and lung tissue was time-dependent. (Source: Environmental Toxicology and Pharmacology)
Source: Environmental Toxicology and Pharmacology - March 24, 2017 Category: Environmental Health Source Type: research

Effects of puerarin on intracellular Ca2+ and cell viability of MDCK renal tubular cells
This study examined whether puerarin affected Ca2+ physiology in MDCK renal tubular cells. Cytosolic free Ca2+ levels ([Ca2+]i) were measured using the fluorescent dye fura-2. Cell viability was examined by using WST-1 assay. Puerarin induced [Ca2+]i rises and the response was reduced by removing extracellular Ca2+. Puerarin-induced Ca2+ entry was not altered by protein kinase C (PKC) activity, but was inhibited by nifedipine. In Ca2+-free medium, treatment with the endoplasmic reticulum Ca2+ pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) or thapsigargin partly inhibited puerarin-evoked [Ca2+]i rises. Inhibition of pho...
Source: Environmental Toxicology and Pharmacology - March 24, 2017 Category: Environmental Health Source Type: research

Toxicity and accumulation of Copper Oxide (CuO) Nanoparticles in different life stages of Artemia salina
Publication date: Available online 22 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): M.R. Madhav, S. Einstein Mariya David, R.S. Suresh Kumar, J.S. Swathy, M. Bhuvaneshwari, Amitava Mukherjee, N. Chandrasekaran Metal nanoparticles production rate and its applications have raised concerns about their release and toxicity to the aquatic and terrestrial organisms. The primary size of Copper Oxide nanoparticles (CuO NP’s) was found to be 114±36nm using Scanning Electron Microscopy (SEM) and a significant increase in the hydrodynamic diameter of CuO NP was seen within 1h of interaction. The...
Source: Environmental Toxicology and Pharmacology - March 23, 2017 Category: Environmental Health Source Type: research

Recent Advances in the Study of 11 β-Hydroxysteroid Dehydrogenase Type 2 (11β-HSD2)Inhibitors
Publication date: Available online 21 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Chunchun Zhou, Fan Ye, He Wu, Hui Ye, Quanxu Chen 11β-Hydroxysteroid dehydrogenase (11β-HSD), which interconverts hormonally active cortisol and inactive cortisone in multiple human tissues, has two distinct isoforms named 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) and 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2). 11β-HSD2 is an NAD+-dependent oxidase which lowers cortisol by converting it to cortisone while 11β-HSD1 mainly catalyzes the reduction which converts cortisone...
Source: Environmental Toxicology and Pharmacology - March 22, 2017 Category: Environmental Health Source Type: research

Severe Camphor Poisoning, A Seven-Year Observational Study
Publication date: Available online 21 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Mitra Rahimi, Fatemeh Shokri, Hossein Hassanian-Moghaddam, Nasim Zamani, Abdolkarim Pajoumand, Shahin Shadnia In a retrospective case series from 2007-2014, we searched for any accidental/intentional, and recreational cases of pure camphor poisoning through hospital records. Epidemiological data, as well as factors correlated with seizure, were evaluated. Thirty cases including 29 males were recruited with a median age of 18 years (range; 0.2 to 87). Patient’s reported ingestion rate of camphor was 1.5 to 15 ...
Source: Environmental Toxicology and Pharmacology - March 22, 2017 Category: Environmental Health Source Type: research

18 β-Glycyrrhetinic acid, the major bioactive component of Glycyrrhizae Radix, attenuates airway inflammation by modulating Th2 cytokines, GATA-3, STAT6, and Foxp3 transcription factors in an asthmatic mouse model
Publication date: Available online 20 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Seung-Hyung Kim, Jung-hee Hong, Ji-Eun Lee, Young-Cheol Lee 18β-Glycyrrhetinic acid (18Gly), the major bioactive component of Glycyrrhizae Radix, possesses anti-ulcerative, anti-inflammatory, and other pharmacological properties. Although 18Gly is associated with immunoregulatory functions of allergic diseases, the pathophysiological mechanisms of 18Gly action in allergic inflammatory lung disease have not been examined. Moreover, there are no in vivo studies on the anti-asthmatic effects of 18Gly in allergic ...
Source: Environmental Toxicology and Pharmacology - March 21, 2017 Category: Environmental Health Source Type: research

Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats
Publication date: Available online 20 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Lawrence A. Olatunji, Kehinde S. Olaniyi, Taofeek O. Usman, Bilikis A. Abolarinwa, Caleb J. Achile, In-kyeom Kim Combined oral contraceptive (COC) use or inhibition of nitric oxide (NO) synthesis has been shown to cause hypertension and insulin resistance. However, the concomitant effects of COC and NO deficiency on the heart and glucose regulation are not well known. We therefore hypothesized that COC treatment during NO deficiency would lead to the development of cardiac hypertrophy that is associated with aggrav...
Source: Environmental Toxicology and Pharmacology - March 21, 2017 Category: Environmental Health Source Type: research

The effect of ozone on the expression of metallothionein in tissues of rats chronically exposed to cadmium.
Publication date: Available online 18 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Halina Milnerowicz, Mariola Śliwińska-Mossoń, Krzysztof A. Sobiech Our aims were to evaluate the expression of metallothionein (MT) in an experimental rat model which experienced chronic exposure to cadmium (Cd) and to measure its expression after ozone therapy (OT) or oxygen (Ox) in the same model, as compared to the control group, which was exposed to neither cadmium nor ozone. Forty male Wistar rats were divided into 5 groups: control, Cd, Cd and Ox, Cd and Oz, and Oz. During our research, Cd concentration (A...
Source: Environmental Toxicology and Pharmacology - March 19, 2017 Category: Environmental Health Source Type: research

Limnothrix sp. KO05: A newly characterized cyanobacterial biosorbent for cadmium removal: The enzymatic and non-enzymatic antioxidant reactions to cadmium toxicity
In this study, we isolated five indigenous cyanobacterial strains from different aqueous environments, with heavy metals contamination, in East Azerbaijan Province (northwest portion of Iran). A strain was identified by morphological and 16S rRNA sequence analysis as Limnothrix sp. KO05 and selected for further studies as having the greatest potential for cadmium uptake. Scanning electron microscopy (SEM) demonstrated cyanobacterium Limnothrix sp. KO05 forms filamentous structures and is straight or curved to some extent. The utmost biosorption capacity was found to be 82.18±1.22mgg−1 at a Cd (II) concentratio...
Source: Environmental Toxicology and Pharmacology - March 19, 2017 Category: Environmental Health Source Type: research

Industrial release of Fluoroquinolones (FQs) in the waste water bodies with their associated ecological risk in Pakistan
Publication date: Available online 15 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Luqman Riaz, Tariq Mahmood, Atif Kamal, Mateen Shafqat, Audil Rashid The unchecked use of Fluoroquinolones (FQs) for the treatment of infections in human and livestock is increasing in Pakistan. In the current study, the prevalence and associated ecological risk of three FQs were investigated in waste-water bodies and sludge samples of Kahuta and Hattar industrial zones. The average concentrations of ciprofloxacin (CIP), enrofloxacin (ENR) and levofloxacin (LEV) in the waste-water samples were slightly higher in Ka...
Source: Environmental Toxicology and Pharmacology - March 16, 2017 Category: Environmental Health Source Type: research

Toxic effect of cadmium adsorbed by different sizes of nano-hydroxyapatite on the growth of rice seedlings
Publication date: Available online 14 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Yifan Huang, Weiwen Qiu, Zhihong Yu, Zhengguo Song Information regarding the toxic effects of cadmium (Cd) adsorbed by nano-hydroxyapatite (NHAP-Cd) on the growth of crop plants remain limited. We investigated the mechanism of NHAP-Cd (diameters, 20 and 40nm; NHAP20-Cd and NHAP40-Cd, respectively) phytotoxicity. Rice seedlings treated with Cd and NHAP20-Cd showed more severe growth retardation compared to those treated with NHAP40-Cd, for the same Cd concentration. Transmission electron microscopy revealed NHAP in ...
Source: Environmental Toxicology and Pharmacology - March 15, 2017 Category: Environmental Health Source Type: research

Cadmium and α-lipoic acid activate similar de novo synthesis and recycling pathways for glutathione balance
This study aimed to investigate de novo synthesis and recycling pathways for GSH balance by different Cd2+ concentrations and ALA in HepG2 cells. ALA activates Nrf2 pathway leading to GSH increment. Pre-treatment with 1μM Cd2+ or ALA produces tolerance to 5μM Cd2+ toxic effects. 5μM Cd2+ exposure significantly augmented nuclear Nrf2, GSH and GCLC, GCLM, HMOX1, TNFα and IL-6 mRNA expression but not GSR, however these upsurges were significantly abrogated by ALA or 1μM Cd2+ pre-treatments. Exposure to low Cd2+ concentration generate timely protective responses, similar to that elicited by ALA, maintaining a...
Source: Environmental Toxicology and Pharmacology - March 10, 2017 Category: Environmental Health Source Type: research

Nrf2 Is Crucial For The Down-Regulation Of Cyp7a1 Induced By Arachidonic Acid In Hepg2 Cells
In conclusion, Nrf2 plays a significant role in the down-regulation of CYP7A1 induced by AA in HepG2 cells.Abbreviations: AA, arachidonic acid; CYP7A1, cholesterol 7α-hydroxylase; FA, fatty acid; HFD, high-fat diet; NAFLD, non-alcoholic fatty liver disease; Nrf2, nuclear factor erythroid-derived 2-like 2; siRNA, small interfering RNA. (Source: Environmental Toxicology and Pharmacology)
Source: Environmental Toxicology and Pharmacology - March 8, 2017 Category: Environmental Health Source Type: research

Human exposure to endocrine disrupting chemicals: effects on the male and female reproductive system
Publication date: Available online 6 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Stavros Sifakis, Vasilis P. Androutsopoulos, Aristeidis M. Tsatsakis, Demetrios A. Spandidos Endocrine disrupting chemicals (EDCs) comprise a group of chemical compounds that have been examined extensively due to the potential harmful effects in the health of human populations. During the past decades particular focus has been given to the harmful effects of EDCs to the reproductive system. The estimation of human exposure to EDCs can be broadly categorized into occupational and environmental exposure, and has been ...
Source: Environmental Toxicology and Pharmacology - March 7, 2017 Category: Environmental Health Source Type: research

Investigating the affinity of BDE154 and 3OH-BDE154 with HSA: Experimental and simulation validation
Publication date: Available online 6 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Wu Yang, Lulu Yang, Zhongsheng Yi, Zhiwei Wu, Jinfang Nie, Aiqian Zhang The physicochemical properties of polybrominated diphenyl ethers are important for modeling their transport, but these data are often missing. Here, satisfactory bioactivity results were obtained using human serum albumin as the carrier, 2,2′,4,4′,5,6′-hexabromodiphenyl ether (BDE154) and 3-hydroxy-2,2′,4,4′, 5,6′-hexabromodiphenyl ether (3OH-BDE154) as the ligands, using UV–visible absorbance, fluoresce...
Source: Environmental Toxicology and Pharmacology - March 7, 2017 Category: Environmental Health Source Type: research

Synthesis and Toxicity Assessment of 3-oxobutanamides against Human Lymphocytes and Isolated Mitochondria
Publication date: Available online 7 March 2017 Source:Environmental Toxicology and Pharmacology Author(s): Nima Razzaghi-Asl, Enaytollah Seydi, Radin Alikhani, Saba Rezvani, Ramin Miri, Ahmad Salimi To reduce costly late-phase compound scrubbing, there has been an increased focus on assessing compounds within in vitro assays that predict properties of human safety liabilities, before preclinical in vivo studies. The aim of our study was to answer the questions that whether the toxicity risk of a series of 3-oxobutanamide derivatives could be predicted by using of human lymphocytes and their isolated mitochondria. Using b...
Source: Environmental Toxicology and Pharmacology - March 7, 2017 Category: Environmental Health Source Type: research

Secreted phosphoprotein 24kD (Spp24) inhibits growth of hepatocellular carcinoma in vivo
In this study, we observed the inhibitory effect of spp24 on hepatocellular carcinoma in vivo. A subcutaneous hepatocellular carcinoma mice model was established by using Hep G2 cells. After sacrifice at day 40, tumor growth was assessed and tumor cell apoptosis and tumor cells proliferation were assessed by TUNEL assay and immunochemical analysis, respectively. BMP2 slightly stimulated the subcutaneous tumor growth compared with the control. Spp24 significantly inhibited the tumor growth and also abolished the BMP2-induced tumor growth (p<0.05). TUNEL assay and immunochemical analysis further showed that spp24 coul...
Source: Environmental Toxicology and Pharmacology - March 5, 2017 Category: Environmental Health Source Type: research

The role of Ntcp, Oatp2, Bsep and Mrp2 in liver injury induced by Dioscorea bulbifera L. and Diosbulbin B in mice
Publication date: Available online 28 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Xiao-Yu Qu, Li-Na Tao, Si-Xi Zhang, Jing-Meng Sun, Jun-Qi Niu, Yan-Hua Ding, Yan-Qing Song Dioscorea bulbifera L. (DB) is a traditional Chinese herb used in thyroid disease and cancer. However, the clinical use of DB remains a challenge due to its hepatotoxicity, which is caused, in part, by the presence of Diosbulbin B (DIOB), a toxin commonly found in DB extracts. As abnormal expression of hepatobiliary transporters plays an important role in drug-induced liver injury, we assessed the hepatotoxicity induced by...
Source: Environmental Toxicology and Pharmacology - March 1, 2017 Category: Environmental Health Source Type: research

Lower concentrations of receptor for advanced glycation end products and epiregulin in amniotic fluid correlate to chemically induced cleft palate in mice
This study investigated the correlation between differentially expressed proteins in amniotic fluid (AF) and cleft palate induced by all-trans retinoic acid (atRA), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice. Seven proteins were differentially expressed at embryonic day (E) 16.5 in atRA and control groups as revealed by label-based mouse antibody array. Enzyme-linked immunosorbent assay was further used to detect the expression levels of these proteins in AF from E13.5 to E16.5 in atRA, TCDD, and control groups. The cleft palate groups showed lower concentrations of receptor for advanced glycation end products ...
Source: Environmental Toxicology and Pharmacology - March 1, 2017 Category: Environmental Health Source Type: research

Regeneration of glutathione by α-lipoic acid via Nrf2/ARE signaling pathway alleviates cadmium-induced HepG2 cell toxicity
Publication date: Available online 27 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Jiayu Zhang, Xue Zhou, Wenbo Wu, Jiachun Wang, Hong Xie, Zhigang Wu Alpha-lipoic acid (α-LA) is an important antioxidant that is capable of regenerating other antioxidants, such as glutathione (GSH). However, the underlying molecular mechanism by which α-LA regenerates GSH remains poorly understood. The current study aimed to investigate whether α-LA regenerates GSH by activation of Nrf2 to alleviate cadmium-induced cytotoxicity in HepG2 cells. In the present study, we found that cadmium induce...
Source: Environmental Toxicology and Pharmacology - February 28, 2017 Category: Environmental Health Source Type: research

Hepatotoxicity and proteomic mechanism of Di-n-butyl-di-(4-chlorobenzohydroxamato)tin(IV) (DBDCT) in vivo
Publication date: Available online 24 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Li Tang, Jie-ran Luo, Yun-lan Li, Rui Ge, Qing-shan Li Di-n-butyl-di-(4-chlorobenzohydroxamato)tin(IV) (DBDCT) is an anti-tumour organotin(IV) compound with hepatotoxicity. To investigate the hepatotoxicity and mechanisms of DBDCT in vivo, proteomic technology 2D gel combined with MALDI-TOF-MS was used in our research. Results indicated that DBDCT increased AST, AKP and ACP activities and decreased ALT activity. Further, sporadic eosinophilic changes and nuclear pyknosis were visible in hepatic pathological obse...
Source: Environmental Toxicology and Pharmacology - February 24, 2017 Category: Environmental Health Source Type: research

Tyrosine assisted size controlled synthesis of silver nanoparticles and their catalytic, in-vitro cytotoxicity evaluation
Publication date: Available online 23 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Sireesh babu Maddinedi, Badal Kumar Mandal, Kiran Kumar Anna A simple, green approach for the size controllable preparation of silver nanoparticles (SNPs) using tyrosine as reducing and capping agent is shown here. The size of SNPs is controlled by varying the pH of tyrosine solution. The as synthesized SNPs are characterized by using XRD, UV-Vis, DLS, TEM and SAED. Zeta potential measurements revealed the stability of tyrosine capped silver nanocolloids. Furthermore, catalytic activity studies concluded that th...
Source: Environmental Toxicology and Pharmacology - February 23, 2017 Category: Environmental Health Source Type: research

Chrysin and baicalin in diabetic nephropathy
Publication date: Available online 22 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Salvatore Chirumbolo, Geir Bjørklund (Source: Environmental Toxicology and Pharmacology)
Source: Environmental Toxicology and Pharmacology - February 22, 2017 Category: Environmental Health Source Type: research

Effect of ammonia on the gene expression levels of the freshwater cyclopoid Eucyclops serrulatus
In this study, the response of stress-related genes to a sublethal ammonia concentration has been investigated in the adults of the freshwater cyclopoid Eucyclops serrulatus. Two short-term exposures (12h and 24h) at 12mg/L NH4 + have been tested. Results indicate that 12mg/L NH4 + causes a significant increase in the expression of some proteins, namely CAT, HSP90 and HSP40, suggesting an activation of the protecting antioxidant system after both 12h and 24h. (Source: Environmental Toxicology and Pharmacology)
Source: Environmental Toxicology and Pharmacology - February 21, 2017 Category: Environmental Health Source Type: research

Effects of octylphenol on the expression of StAR, CYP17 and CYP19 in testis of Rana chensinensis
Publication date: Available online 21 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Yao Bai, Xin-Yi Li, Zhi-Jun Liu, Yu-Hui Zhang It has been proposed that a decline in sperm quality is associated with exposure to environmental chemicals with estrogenic activity. Seeking possible explanations for this effect, this study investigated the effects of octylphenol (OP) on the synthesis of steroid hormones in amphibian. Rana chensinensis were exposed to 10-8, 10-7 and 10-6 mol/L OP after 10, 20, 30 and 40 days. The cDNA fragments of StAR (274bp), CYP17 (303bp) and CYP19 (322bp) were cloned. In situ h...
Source: Environmental Toxicology and Pharmacology - February 21, 2017 Category: Environmental Health Source Type: research

Acute toxicity, biochemical toxicity and genotoxicity caused by 1-butyl-3-methylimidazolium chloride and 1-butyl-3-methylimidazolium tetrafluoroborate in zebrafish (Danio rerio) livers
Publication date: Available online 21 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Cheng Zhang, Yuting Shao, Lusheng Zhu, Jinhua Wang, Jun Wang, Yingying Guo The present study examined the potential toxicity of 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and 1-butyl-3- methylimidazolium tetrafluoroborate ([Bmim]BF4) in the liver cells of zebrafish (Danio rerio) with different doses (20–160mg/L) on 7 and 14 days. The effects of [Bmim]Cl and [Bmim]BF4 on acute toxicity, reactive oxygen species (ROS), antioxidant enzymes, glutathione S-transferase (GST), malondialdehyde (MDA), and DNA d...
Source: Environmental Toxicology and Pharmacology - February 21, 2017 Category: Environmental Health Source Type: research

Zinc oxide nanoparticles hepatotoxicity: Histological and histochemical study
Publication date: Available online 16 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Mansour I. Almansour, Mosaid A. Alferah, Ziad A. Shraideh, Bashir M. Jarrar Zinc oxide nanoparticles (ZnO NPs) are widely used in industry and cosmetic products with promising investment in medical diagnosis and treatment. However, these particles may reveal a high potential risk for human health with no information about hepatotoxicity that might be associated with their exposure. The present work was carried out to investigate the histological and histochemical alterations induced in the hepatic tissues by nak...
Source: Environmental Toxicology and Pharmacology - February 17, 2017 Category: Environmental Health Source Type: research

Sinapic acid ameliorate cadmium-induced nephrotoxicity: In vivo possible involvement of oxidative stress, apoptosis, and inflammation via NF- κB downregulation
Publication date: Available online 15 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Mushtaq Ahmad Ansari, Mohammad Raish, Ajaz Ahmad, Khalid M. Alkharfy, Sheikh Fayaz Ahmad, Sabry M. Attia, Abdulaziz M.S. Alsaad, Saleh A. Bakheet Cadmium (CD), an environmental and industrial pollutant, generates reactive oxygen species (ROS) and NOS responsible for oxidative and nitrosative stress that can lead to nephrotoxic injury, including proximal tubule and glomerulus dysfunction. Sinapic acid (SA) has been found to possess potent antioxidant and anti-inflammatory effects in vitro and in vivo. We aimed to...
Source: Environmental Toxicology and Pharmacology - February 16, 2017 Category: Environmental Health Source Type: research

Acetyl-l-carnitine partially prevents benzene-induced hematotoxicity and oxidative stress in C3H/He mice
Publication date: Available online 13 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Rongli Sun, Juan Zhang, Haiyan Wei, Xing Meng, Qin Ding, Fengxia Sun, Meng Cao, Lihong Yin, Yuepu Pu Benzene is an environmental pollutant and occupational toxicant which induces hematotoxicity. Our previous metabonomics study suggested that acetyl-l-carnitine (ALCAR) decreased in the mouse plasma and bone marrow (BM) cells due to benzene exposure. In the present study, the topic on whether ALCAR influences hematotoxicity caused by benzene exposure was explored. Thirty-two male C3H/He mice were divided into four...
Source: Environmental Toxicology and Pharmacology - February 13, 2017 Category: Environmental Health Source Type: research

Environmental epigenomics: Current approaches to assess epigenetic effects of endocrine disrupting compounds (EDC ’s) on human health
Publication date: Available online 10 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Natalia Tapia-Orozco, Gerardo Santiago-Toledo, Valeria Barrón, Ana María Espinosa-García, José Antonio García-García, Roeb García-Arrazola Environmental Epigenomics is a developing field to study the epigenetic effect on human health from exposure to environmental factors. Endocrine disrupting chemicals have been detected primarily in pharmaceutical drugs, personal care products, food additives, and food containers. Exposure to endocrine-disrupting chemicals (EDCs...
Source: Environmental Toxicology and Pharmacology - February 11, 2017 Category: Environmental Health Source Type: research

27-hydroxycholesterol induces invasion and migration of breast cancer cells by increasing MMP9 and generating EMT through activation of STAT-3
Publication date: Available online 10 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Zhaoxia Shen, Dongmei Zhu, Jiao Liu, Juan Chen, Yun Liu, Chunyan Hu, Zhong Li, Yuan Li Breast carcinoma plays a vital role in the reasons of global women's death. ER-related invasion and migration play an important part in the development and prognosis of breast cancer. Here, we found that 27-Hydroxycholesterol (27HC) could induce epithelial-mesenchymal transition (EMT) and increase the expression of the matrix metalloproteinase 9 (MMP9) at mRNA level and the active form. Meanwhile, interestingly, we found 27HC ...
Source: Environmental Toxicology and Pharmacology - February 11, 2017 Category: Environmental Health Source Type: research

Aucubin and its hydrolytic derivative attenuate activation of hepatic stellate cells via modulation of TGF- & lt;beta & gt; stimulation
Publication date: Available online 8 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Pei-Yu Lv, Han Feng, Wei-Hua Huang, Ying-Ying Tian, Ya-Qin Wang, Yu-hua Qin, Xiao-Hui Li, Kai Hu, Hong-Hao Zhou, Dong-Sheng Ouyang Eucommia ulmoides is an important traditional Chinese medicine and has been used as a tonic with a long history. Aucubin is an active component extracted from Eucommia ulmoides, which has liver-protection effects. However the mechanisms are still unclear. To investigate the inhibitory effects and the underlying mechanisms of aucubin on TGF-<beta>1-induced activation of h...
Source: Environmental Toxicology and Pharmacology - February 8, 2017 Category: Environmental Health Source Type: research

Exposure to PM2.5 induces aberrant activation of NF- κB in human airway epithelial cells by downregulating miR-331 expression
Publication date: Available online 6 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Lei Song, Dan Li, Xiaoping Li, Lianjun Ma, Xiaoxue Bai, Zhongmei Wen, Xiufang Zhang, Dong Chen, Liping Peng Exposure to particulate matter (PM) with an aerodynamic diameter≤2.5μm (PM2.5) induces reactive oxygen species (ROS) and pro-inflammatory cytokine production, leading to airway epithelial injury. However, the mechanisms underlying the toxicity of PM2.5 have not been clarified. Here, we show that exposure to PM2.5 induces sustained activation of the nuclear factor kappa B (NF-κB) signaling in hum...
Source: Environmental Toxicology and Pharmacology - February 7, 2017 Category: Environmental Health Source Type: research

Ascorbic acid ameliorates behavioural deficits and neuropathological alterations in rat model of Alzheimer ’s disease
Publication date: Available online 6 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Olayemi Joseph Olajide, Emmanuel Olusola Yawson, Ismail Temitayo Gbadamosi, Tolulope Timothy Arogundade, Ezra Lambe, Kosisochukwu Obasi, Ismail Temitayo lawal, Abdulmumin Ibrahim, Kehinde Yomi Ogunrinola Exploring the links between neural pathobiology and behavioural deficits in Alzheimer’s disease (AD), and investigating substances with known therapeutic advantages over subcellular mechanisms underlying these dysfunctions could advance the development of potent therapeutic molecules for AD treatment. Here ...
Source: Environmental Toxicology and Pharmacology - February 7, 2017 Category: Environmental Health Source Type: research

Melamine and/or formaldehyde exposures affect steroidogenesis via alteration of StAR protein and testosterone synthetic enzyme expression in male mice
Publication date: March 2017 Source:Environmental Toxicology and Pharmacology, Volume 50 Author(s): Samah R. Khalil, Ashraf Awad, Sozan A. Ali The reproductive effects of melamine and formaldehyde, either alone or in combination, on mature male Swiss mice were investigated. The animals were orally administered melamine (50mg/kg/day), formaldehyde (25mg/kg/day), a mixture of melamine and formaldehyde, or a vehicle control for 65 consecutive days. As a result, the deterioration of sperm characteristics and inhibition of testicular enzyme activity were observed in the melamine- and formaldehyde-exposed groups. In addition, t...
Source: Environmental Toxicology and Pharmacology - February 7, 2017 Category: Environmental Health Source Type: research

Silver nanoparticles alter the permeability of sheep pleura and of sheep and human pleural mesothelial cell monolayers.
Publication date: Available online 6 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Zoi V. Arsenopoulou, Ioannis A. Taitzoglou, Paschalis-Adam Molyvdas, Konstantinos I. Gourgoulianis, Chrissi Hatzoglou, Sotirios G. Zarogiannis Nanoparticles have been implicated in the development of pleural effusions in exposed factory workers while in experimental animal studies it has been shown that they induce inflammation, fibrosis and carcinogenesis in the pleura. The scope of this study was to investigate the direct effects of silver nanoparticles exposure on the membrane permeability of sheep parietal pl...
Source: Environmental Toxicology and Pharmacology - February 6, 2017 Category: Environmental Health Source Type: research

Impairment in the mesohippocampal dopamine circuit following exposure to the brominated flame retardant, HBCDD
Publication date: Available online 4 February 2017 Source:Environmental Toxicology and Pharmacology Author(s): Camille Pham-Lake, Elizabeth B. Aronoff, Chad R. Camp, Aimee Vester, Sam J. Peters, W.Michael Caudle Many chemicals have been used to increase the safety of consumer products by reducing their flammability and risk for ignition. Recent focus on brominated flame retardants, such as polybrominated diphenyl ethers (PBDEs) has shown them to contribute to neurobehavioral deficits in children, including learning and memory. As the manufacture and use of PBDEs has been reduced, replacement chemicals, such as hexabromocy...
Source: Environmental Toxicology and Pharmacology - February 5, 2017 Category: Environmental Health Source Type: research

Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study
In conclusion, linagliptin/calcipotriol combination may represent an effective therapeutic modality for amelioration of bleomycin-induced pulmonary fibrosis. Graphical abstract (Source: Environmental Toxicology and Pharmacology)
Source: Environmental Toxicology and Pharmacology - February 5, 2017 Category: Environmental Health Source Type: research