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Improvement of ethanol production from birch and spruce pretreated with 1-H-3-methylmorpholinium chloride
ConclusionsThis study demonstrated the ability of [HMMorph][Cl] as an inexpensive agent to pretreat softwood as well as hardwood. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Synthesis, characterization, and in vitro evaluation of gamma radiation-induced PEGylated isoniazid
ConclusionsIn summary, the research suggests that it is possible to covalently attach the PEG onto INH by the proposed method and to obtain a slow-acting isoniazid derivative with little toxicity in vitro and higher anti-mycobacterial potency than the neat drug. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Cloning and molecular characterization of a putative habanero pepper SERK1 cDNA expressed during somatic and zygotic embryogenesis
ConclusionDNA sequence alignments and gene expression patterns suggest that CchSERK1 is the C. chinense SERK1 homolog. Significant levels of CchSERK1 transcripts were found in tissues with cell differentiation activities like vascular axes and during the development of zygotic and somatic embryos. These results suggest that CchSERK1 might have regulatory functions in cell differentiation and could be used as a biotechnological tool to study the peppers recalcitrance to proliferate in vitro. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Bacterial community structure in soils contaminated with electronic waste pollutants from Delhi NCR, India
ConclusionsThis is the first report describing the bacterial community structure of composite soil samples of e-waste contaminated sites of Loni and Mandoli, Delhi NCR, India. The findings indicate that novel bacteria having potential bioremediating properties may be present in the e-waste contaminated sites and need to be explored further. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Retrovectors packaged in CHO cells to generate GLP-1-fc stable expression CHO cell lines
ConclusionsSuspension CHO cells are clean, safe, and reliable cells for retrovector packaging. Retrovectors packaged from this system could be used to generated CHO stable cell lines for recombinant protein expression. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 3, 2019 Category: Biotechnology Source Type: research

Adaptive evolution and selection of stress-resistant Saccharomyces cerevisiae for very high-gravity bioethanol fermentation
ConclusionsA multiple-stress-tolerant yeast clone was obtained using adaptive evolution by a freeze–thaw method coupled with stress shock selection. The selected robust yeast strain exhibits potential for bioethanol production through VHG fermentation. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 20, 2019 Category: Biotechnology Source Type: research

A novel thermophilic exo-β-1,3-glucanase from the thermophile biomass-degrading fungus Thielavia terrestris Co3Bag1. Purification and biochemical characterization of the enzyme
ConclusionsThis report thus describes the purification and characterization of TtBgnA, a novel exo-β-1,3-glucanase GH-31 family from the thermophilic fungus T. terrestris Co3Bag1. Based on the biochemical properties displayed by TtBgnA, the enzyme could be considered as a candidate for potential biotechnological applications. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 18, 2019 Category: Biotechnology Source Type: research

Discovery and molecular analysis of conserved circRNAs from cashmere goat reveal their integrated regulatory network and potential roles in secondary hair follicle
ConclusionsA total of eighteen circular RNAs were identified and characterized from skin tissue of cashmere goat. The eight up-regulated circular RNAs at anagen might have significant roles in secondary hair follicle of cashmere goat. Our results would provide novel regulatory layer to elucidating the molecular mechanisms underlying the development of secondary hair follicle and the growth of cashmere fiber in cashmere goat. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 10, 2019 Category: Biotechnology Source Type: research

Adaptive evolution and selection of stress-resistant Saccharomyces cerevisiae for very high gravity bioethanol fermentation
ConclusionsA multiple-stress-tolerant yeast clone was obtained using adaptive evolution via a freeze–thaw method coupled with stress shock selection. The selected robust yeast strain exhibits potential for bioethanol production via VHG fermentation. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 10, 2019 Category: Biotechnology Source Type: research

An efficient method of Yarrowia lipolytica immobilization using oil- and emulsion-modified bacterial cellulose carrier
ConclusionThe application of oil- or emulsion-modified BC as carriers significantly increased efficiency of Y. lipolytica immobilization as compared to unmodified BC. The addition of oil or emulsion to the culture medium can be a simple but effective method to modify the porosity of BC-based carriers. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 3, 2019 Category: Biotechnology Source Type: research

Le arning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection
Publication date: Available online 27 June 2019Source: Electronic Journal of BiotechnologyAuthor(s): Ariel D. Arencibia, Vívian D'Afonseca, Mohan Chakravarthi, Stefano CastiglioneAbstractWe highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants, tran...
Source: Electronic Journal of Biotechnology - June 28, 2019 Category: Biotechnology Source Type: research

Learning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection
Publication date: Available online 27 June 2019Source: Electronic Journal of BiotechnologyAuthor(s): Ariel D. Arencibia, Vívian D'Afonseca, Mohan Chakravarthi, Stefano CastiglioneAbstractWe highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants, tran...
Source: Electronic Journal of Biotechnology - June 27, 2019 Category: Biotechnology Source Type: research

Co-production of ethanol and biodiesel from sweet sorghum juice in two consecutive fermentation steps
ConclusionThe two step process is viable and could be considered an integral part of a consolidated biorefinery from sweet sorghum. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - June 25, 2019 Category: Biotechnology Source Type: research

Assessment of wasteland derived biomass for bioethanol production
ConclusionsThe plants such as fireweed, common broom, hay and goldenrod may be useful for the production of liquid biofuels and it would be necessary in the further stage of research to establish and optimize the conditions for the technology of ethyl alcohol producing from these plant species. Enzymatic hydrolysis of biomass from agricultural wastelands results in a large increase in fermentable sugars, comparable to the enzymatic hydrolysis of rye, wheat, rice or maize straw. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 24, 2019 Category: Biotechnology Source Type: research

Enzymatic preparation of fructooligosaccharides-rich burdock syrup with enhanced antioxidative properties
ConclusionsOur results could be applied to the development of efficient extraction of valuable products from agricultural materials using enzyme-mediated methods. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 15, 2019 Category: Biotechnology Source Type: research