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Point-of-care testing for CXCL13 in Lyme neuroborreliosis
Cerebrospinal fluid CXCL13 is a marker for Lyme neuroborreliosis (LNB). CXCL13 lateral flow immunoassay (LFA) was compared to CXCL13 ELISA. CXCL13 LFA results correlated strongly with CXCL13 ELISA results. CXCL13 LFA is a rapid and easy to perform test, which is suitable for routine point-of-care diagnostics of suspected LNB patients. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 23, 2018 Category: Microbiology Authors: Annukka Pietik äinen, Jarmo Oksi, Jukka Hytönen Tags: Note Source Type: research

Dissemination of carbapenem-resistant Klebsiella pneumoniae harboring KPC-carrying plasmid pKPC_P16, a pKPC_LK30 variant, in northern Taiwan
The prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) was up to 30% between 2014 and 2016 in the study hospital. Of these 77 CRKP isolates, 22 isolates with sequence type ST11 carried the new pKPC_P16 plasmid, a pKPC_LK30 variant, and were widely disseminated between 2014 and 2015 in northern Taiwan. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 21, 2018 Category: Microbiology Authors: Jiann-Yuan Chen, Ming-Li Liou, Han-Yueh Kuo, Chia-Wei Lu, Yi-Chieh Lai, Yun-Yu Lin, Chang-Hua Chen Tags: Note Source Type: research

Dissemination of carbapenem-resistant Klebsiella pneumoniae harboring KPC-carrying plasmid pKPC_P16, a pKPC_LK30 variant, in northern Taiwan
The prevalence of carbapenem-resistant K. pneumoniae (CRKP) was up to 30% between 2014 and 2016 in the study hospital. Of these 77 CRKP isolates, 22 isolates with sequence type ST11 carried the new pKPC_P16 plasmid, a pKPC_LK30 variant, and were widely disseminated between 2014 and 2015 in northern Taiwan. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 21, 2018 Category: Microbiology Authors: Jiann-Yuan Chen, Ming-Li Liou, Han-Yueh Kuo, Chia-Wei Lu, Yi-Chieh Lai, Yun-Yu Lin, Chang-Hua Chen Tags: Note Source Type: research

Determination of the antimicrobial susceptibility and molecular profile of clarithromycin resistance in the Mycobacterium abscessus complex in Japan by variable number tandem repeat analysis
Mycobacterium abscessus complex, including three subspecies —M. abscessus, M. massiliense, and M. bolletii—is resistant to a variety of antibiotics so limited treatment options are available. The susceptibility of these subspecies to antimicrobial agents depends in particular on the erm(41) sequevar and rrl mutations in the 23S rRNA, which are potentiall y related to clarithromycin (CLR) resistance. The purpose of this study was to carry out identification and molecular characterization of these subspecies based on variable number of tandem repeats (VNTR) analysis. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 18, 2018 Category: Microbiology Authors: Mari Kusuki, Kayo Osawa, Kentaro Arikawa, Miho Tamura, Katsumi Shigemura, Toshiro Shirakawa, Tatsuya Nakamura, Yuji Nakamachi, Masato Fujisawa, Jun Saegusa, Issei Tokimatsu Source Type: research

Determination of the antimicrobial susceptibility and molecular profile of clarithromycin resistance in the Mycobacterium abscessus complex in Japan by variable number tandem repeat analysis
Mycobacterium abscessus complex, including three subspecies: M. abscessus, M. massiliense, and M. bolletii, is resistant to a variety of antibiotics so limited treatment options are available. The susceptibility of these subspecies to antimicrobial agents depends in particular on the erm(41) sequevar and rrl mutations in the 23S rRNA, which are potentially related to clarithromycin (CLR) resistance. The purpose of this study was to carry out identification and molecular characterization of these subspecies based on variable number of tandem repeats (VNTR) analysis. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 18, 2018 Category: Microbiology Authors: Mari Kusuki, Kayo Osawa, Kentaro Arikawa, Miho Tamura, Katsumi Shigemura, Toshiro Shirakawa, Tatsuya Nakamura, Yuji Nakamachi, Jun Saegusa, Issei Tokimatsu Source Type: research

Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity
In this study using 3956 control sera, we demonstrated that although this two-tier testing algorithm does significantly improve diagnostic specificity compared with each of the EIAs individually, the WCS EIA and the C6 peptide EIA are not independent tests. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Gary P. Wormser, Claudia R. Molins, Andrew Levin, Susan C. Lipsett, Lise E. Nigrovic, Martin E. Schriefer, John A. Branda Source Type: research

Incidence of matrix genes mutations affecting PCR tests among influenza H3N2 clades circulating during the 2014/15 season
Influenza circulating during the 2014/15 season was associated with significant drift and the emergence of new H3N2 subgroups. It was also known that mutations were accumulating in the WHO-recommended amplification region of the M1 gene, affecting the performance of many commercial polymerase chain reaction assays. However, the prevalence of M1 target mutations was unknown. To investigate this, isolates from the GISAID database from Australia (n=100), Europe (n=473), and the USA (n=1175) were analyzed. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Kathleen A. Stellrecht Source Type: research

Proteus mirabilis harboring carbapenemase NDM-5 and ESBL VEB-6 detected in Austria
We describe a case of carbapenemase-harboring Proteus mirabilis together with detection of NDM-5 in Austria accompanied by other bacterial strains with a wide range of beta-lactamases including OXA-181 and VEB-6. Isolates were obtained from a subphrenic abscess from one patient who was previously treated with broad-spectrum antibiotics in Bangladesh. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Thomas Valentin, Gebhard Feierl, Lilian Masoud-Landgraf, Peter Kohek, Josefa Luxner, Gernot Zarfel Tags: Note Source Type: research

Emergence of multidrug-resistant clones in levofloxacin-nonsusceptible Streptococcus pneumoniae isolates in Korea
The use of fluoroquinolones to treat respiratory tract infections and pneumonia due to Streptococcus pneumoniae has affected the emergence of resistance to this class of drugs. Increasing pneumococcal resistance to levofloxacin has become a major public health concern. We investigated the prevalence and genetic characteristics of levofloxacin-nonsusceptible S. pneumoniae (LNSP) clinical isolates in Korea. A total of 43 LNSP isolates collected from a national surveillance study at 13 tertiary hospitals between 2008 and 2014 were analyzed for serotype and antimicrobial susceptibilities to 19 antimicrobial agents as well as t...
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Jin Yang Baek, Cheol-In Kang, So Hyun Kim, Kwan Soo Ko, Doo Ryeon Chung, Kyong Ran Peck, Nam Yong Lee, Jae-Hoon Song Source Type: research

Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus
Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for respiratory syncytial virus (RSV), and 80% (4/5) for negative specimens. The approximate limit of detection of the MAP was 30 copies/assay for RSV and 1500 copies/assay for Middle East respiratory syndrome coronavirus. (Source: Diagnostic Microbiolog...
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Justin Hardick, David Metzgar, Lisa Risen, Christopher Myers, Melinda Balansay, Trent Malcom, Richard Rothman, Charlotte Gaydos Tags: Note Source Type: research

Evaluation of the Carbapenem Detection Set ™ for the detection and characterization of carbapenemase-producing Enterobacteriaceae
The objective of this study was to assess the performance of the Carbapenemase Detection Set ™ (CDS; Mast Diagnostics) in association with i) the EUCAST meropenem screening cut-off and ii) the faropenem–temocillin algorithm (FTa) for the screening of carbapenemase-producing Enterobacteriaceae (CPE). A total of 200 well-characterized enterobacterial isolates with reduced susceptibility t o at least one carbapenem including 63 non-CPEs and 137 CPEs belonging to different Ambler classes were initially screened for CPEs using i) the EUCAST meropenem cut-off (diameter (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Laurent Dortet, Sandrine Bernabeu, Camille Gonzalez, Thierry Naas Source Type: research

Evaluation of a Sequential Enzyme Immunoassay Testing Algorithm for Lyme Disease Demonstrates Lack of Test Independence but High Diagnostic Specificity
In this study using 3956 control sera, we demonstrated that although this 2-tier testing algorithm does significantly improve diagnostic specificity compared with each of the EIAs individually, the WCS EIA and the C6 peptide EIA are not independent tests. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Gary P. Wormser, Claudia R. Molins, Andrew Levin, Susan C. Lipsett, Lise E. Nigrovic, Martin E. Schriefer, John A. Branda Source Type: research

Incidence of Matrix Genes Mutations Affecting PCR Tests Among Influenza H3N2 Clades Circulating During the 2014/15 Season
Influenza circulating during the 2014/15 season were associated with significant drift and the emergence of new H3N2 subgroups. It was also known that mutations were accumulating in the WHO recommended amplification region of the M1 gene, affecting the performance of many commercial PCR assays. However, the prevalence of M1 target mutations was unknown. To investigate this, isolates from the GISAID database from Australia (n=100), Europe (n=473), and the USA (n=1175) were analyzed. The predominate clade was 3C.2a (75%), with a higher representation among USA isolates (81%). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - February 17, 2018 Category: Microbiology Authors: Kathleen A. Stellrecht Source Type: research