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Detection of oral HPV infection – Comparison of two different specimen collection methods and two HPV detection methods
Very little is known about the natural history of oral HPV infection. Several different methods exist to collect oral specimens and detect HPV, but their respective performance characteristics are unknown. We compared two different methods for oral specimen collection (oral saline rinse and commercial saliva kit) from 96 individuals and then analyzed the samples for HPV by two different PCR detection methods (single GP5+/6+ PCR and nested MY09/11 and GP5+/6+ PCR). For the oral rinse samples, the oral HPV prevalence was 10.4% (GP+ PCR; 10% repeatability) vs 11.5% (nested PCR method; 100% repeatability). (Source: Diagnostic ...
Source: Diagnostic Microbiology and Infectious Disease - December 12, 2017 Category: Microbiology Authors: Marjorie M.A. de Souza, Gunter Hartel, David C. Whiteman, Annika Antonsson Source Type: research

Evaluation of the CellaVision DM96 Advanced RBC Application for screening and follow-up of malaria infection
In this study, the performance of the software for detection of malaria and calculation of parasitemia was evaluated and compared to microscopy (n=40). For CellaVision, both pre- and post-reclassification results were evaluated. Sensitivity was moderate, even post-reclassification (72%), due to low numbers of analyzed RBC and limited resolution of photographs. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 8, 2017 Category: Microbiology Authors: Lisa Florin, Karel Maelegheer, Astrid Muyldermans, Marjan Van Esbroeck, Eric Nulens, Jan Emmerechts Source Type: research

Surveillance of Iclaprim Activity: In Vitro Susceptibility of Gram-positive Pathogens Collected from 2012 to 2014 From the United States, Asia Pacific, Latin American and Europe
Iclaprim is a novel diaminopyrimidine, which inhibits bacterial dihydrofolate reductase, and it is highly active against Gram-positive pathogens including emerging drug-resistant pathogens. In vitro activity of iclaprim and comparators against 2814 Gram-positive clinical isolates from the United States, Asia Pacific, Latin American and Europe collected between 2012 and 2014 were tested. Susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 7, 2017 Category: Microbiology Authors: David B Huang, Thomas M File, Matthew Dryden, G. Ralph Corey, Antoni Torres, Mark H Wilcox Source Type: research

Effective utilization of C. difficile PCR and identification of clinicopathologic factors associated with conversion to a positive result in symptomatic patients
We assess the diagnostic yield of repeat testing for C. difficile using molecular methods within 7days of a negative test and identify specific factors associated with conversion from negative to positive test result within a 7-day period to aid in selective test utilization. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 6, 2017 Category: Microbiology Authors: Mohamed E. Mostafa, Timothy Flynn, Christopher P. Hartley, Nathan A. Ledeboer, Blake W. Buchan Source Type: research

Antibiotic Resistance Rates for Pseudomonas aeruginosa Clinical Respiratory and Bloodstream Isolates Among the Veterans Affairs Healthcare System from 2009 to 2013
Pseudomonas aeruginosa is a major cause of healthcare-associated infections and resistance among isolates is an increasing burden. The study purpose was to describe national resistance rates for clinical P. aeruginosa respiratory and bloodstream cultures and the prevalence of multidrug-resistant (MDR) P. aeruginosa within the Veterans Affairs (VA). MDR was defined as non-susceptibility to at least one drug in at least 3 of the following 5 categories: carbapenems, extended-spectrum cephalosporins, aminoglycosides, and piperacillin/tazobactam. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 6, 2017 Category: Microbiology Authors: Haley J. Appaneal, Aisling R. Caffrey, Lan Jiang, David Dosa, Leonard A. Mermel, Kerry L. LaPlante Source Type: research

Challenges of Francisella classification exemplified by an atypical clinical isolate
We report the complete genome sequence of a clinical isolate, designated here as F. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 6, 2017 Category: Microbiology Authors: LM Matz, KY Kamdar, ME Holder, GA Metcalf, GM Weissenberger, Q Meng, V Vee, Y Han, DM Muzny, RA Gibbs, CL Johnson, PA Revell, JF Petrosino Source Type: research

Presence of Immune Deficiency increases the risk of Hospitalization in Patients with Norovirus Infection
Norovirus is an emerging pathogen causing gastroenteritis. We sought to identify factors associated with clinical outcomes in a cohort of patients with laboratory-confirmed norovirus infection. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 4, 2017 Category: Microbiology Authors: Keith A. Sacco, Thanai Pongdee, Matthew J. Binnicker, Mark Espy, Darrell Pardi, Sahil Khanna, Avni Y. Joshi Source Type: research

Determination of MIC and disk diffusion quality control guidelines for meropenem –vaborbactam, a novel carbapenem/boronic acid β-lactamase inhibitor combination
Meropenem –vaborbactam is a carbapenem/cyclic boronic acid β-lactamase inhibitor combination primarily active against Gram-negative bacilli, including those harboring class A serine carbapenemases such as Klebsiella pneumoniae carbapenemase (KPC). A Clinical and Laboratory Standards Institute M23-A4 (Tier 2) quality control study established broth microdilution and disk diffusion ranges for reference strains. Two KPC-producing K. pneumoniae ATCC strains are recommended for quality control testing. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 3, 2017 Category: Microbiology Authors: Erik Munson, Michael D. Huband, Mariana Castanheira, Kelley A. Fedler, Robert K. Flamm Source Type: research

Determination of MIC and Disk Diffusion Quality Control Guidelines for Meropenem-vaborbactam, a Novel Carbapenem/Boronic Acid β-lactamase Inhibitor Combination
Meropenem-vaborbactam is a carbapenem/cyclic boronic acid β-lactamase inhibitor combination primarily active against Gram-negative bacilli, including those harboring class A serine carbapenemases such as Klebsiella pneumoniae carbapenemase (KPC). A CLSI M23-A4 (tier-2) quality control study established broth microdilution and disk diffusion ranges for ref erence strains. Two KPC-producing K. pneumoniae ATCC strains are recommended for quality control testing. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 3, 2017 Category: Microbiology Authors: Erik Munson, Michael D. Huband, Mariana Castanheira, Kelley A. Fedler, Robert K. Flamm Source Type: research

Evaluation of the Diasorin Liaison ® XL Zika Capture IgM CMIA for Zika virus serological testing
Due to the increase of Zika virus (ZIKV) transmission throughout the world, many commercial kits have recently become available to aid in laboratory diagnosis of ZIKV infections in clinical samples. Here, we analyze the fully-automated Liaison ® XL Zika Capture IgM assay against the recommended IgM-capture enzyme-linked immunosorbent assay. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 2, 2017 Category: Microbiology Authors: Angela Sloan, David Safronetz, Kai Makowski, Nicole Barairo, Charlene Ranadheera, Kristina Dimitrova, Kimberly Holloway, Emelissa Mendoza, Heidi Wood, Mike Drebot, Ainsley Gretchen, Kamran Kadkhoda Tags: Note Source Type: research

Evaluation of the Diasorin Liaison ® XL Zika Capture IgM ELISA for Zika virus serological testing
Due to the increase of Zika virus (ZIKV) transmission throughout the world, many commercial kits have recently become available to aid in laboratory diagnosis of ZIKV infections in clinical samples. Here, we analyze the fully-automated Liaison ® XL Zika Capture IgM assay against the recommended IgM-capture enzyme-linked immunosorbent assay. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - December 2, 2017 Category: Microbiology Authors: Angela Sloan, David Safronetz, Kai Makowski, Nicole Barairo, Charlene Ranadheera, Kristina Dimitrova, Kimberly Holloway, Emelissa Mendoza, Heidi Wood, Mike Drebot, Ainsley Gretchen, Kamran Kadkhoda Tags: Note Source Type: research

A real-time multiplex PCR for the identification and typing of Vibrio cholerae
We report the development and validation of a duo-triplex real-time PCR for the rapid identification and typing of Vibrio cholerae. The PCR assay targets a species specific toxR gene present in all strains of V. cholerae and used as a marker for the species, wbeO1 and wbfO139, encoding the O1 and O139 somatic antigens, and ctxA encoding CT. The two tcpA variants associated with the Classical and El-Tor biotypes are used to infer biotype. The assay was evaluated using 178 isolates comprising eight different Vibrio species, including 122 isolates of V. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 30, 2017 Category: Microbiology Authors: David R Greig, Tadgh J Hickey, Megan D Boxall, Hafsah Begum, Amy Gentle, Claire Jenkins, Marie A Chattaway Source Type: research

Development of a Multiplex TaqMan Real-Time PCR Assay for the Detection of Chlamydia psittaci and Chlamydia pneumoniae in Human Clinical Specimens
Diagnosis of Chlamydia psittaci and Chlamydia pneumoniae infections has traditionally relied on serological assays. We developed a multiplex real-time PCR assay for detection of C. psittaci, C. pneumoniae and an internal control. Results of this assay demonstrated 100% concordance compared to results of previously tested human clinical specimens. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 27, 2017 Category: Microbiology Authors: Bernard J. Wolff, Shatavia S. Morrison, Jonas M. Winchell Tags: Note Source Type: research

Frequency of Small-Colony Variants and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus in Cystic Fibrosis Patients
Small-colony variants (SCVs) are a distinct phenotype of S. aureus, known for their role in chronic, difficult to treat infections, including cystic fibrosis (CF) lung disease. The goal of this study was to characterize SCV MRSA infection in an adult and pediatric CF population and to identify antibiotic susceptibility patterns unique to SCV MRSA. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - November 26, 2017 Category: Microbiology Authors: Nuntra Suwantarat, Mayer Rubin, Latetia Bryan, Tsigereda Tekle, Michael P. Boyle, Karen C. Carroll, Mark T. Jennings Source Type: research

A Novel and Highly Sensitive Real-Time nested RT-PCR Assay in a Single Closed Tube for Detection of Enterovirus
The sensitivity of qRT-PCR assay is not adequate for the detection of the samples with lower viral load, particularly in the cerebrospinal fluid (CSF) of patients. Here, we present the development of a highly sensitive real-time nested RT-PCR (RTN RT-PCR) assay in a single closed tube for detection of human enterovirus (HEV). The clinical performance of both RTN RT-PCR and qRT-PCR was also tested and compared using 140 CSF and fecal specimens.The sensitivities of RTN RT-PCR assay for EV71, Coxsackievirus A (CVA)16, CVA6 and CVA10 achieved 10 −8 dilution with a corresponding Ct value of 38.20,36.45,36.75 and 36.45, respec...
Source: Diagnostic Microbiology and Infectious Disease - November 26, 2017 Category: Microbiology Authors: Xin-xin Shen, Fang-zhou Qiu, Huai-long Zhao, Meng-jie Yang, Liu Hong, Song-tao Xu, Shuai-feng Zhou, Gui-xia Li, Zhi-shan Feng, Xue-jun Ma Source Type: research