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An in vitro evaluation of the efficacy of tedizolid: Implications for the treatment of skin and soft tissue infections
Skin and soft tissue infections (SSTI) are among the most commonly occurring infections and evidence suggests that these are increasing world-wide. The aetiology is diverse, but Staphylococcus aureus predominate and these are often resistant to antimicrobials that were previously effective. Tedizolid is a new oxazolidinone-class antibacterial indicated for the treatment of adults with SSTI caused by Gram-positive pathogens, including S. aureus. The aim of this study was to evaluate the in vitro efficacy of tedizolid in comparison to other clinically used antibacterials against antibiotic sensitive- and resistant-staphyloco...
Source: Diagnostic Microbiology and Infectious Disease - January 10, 2018 Category: Microbiology Authors: Pierre Delpech, Muna ALeryan, Brian Jones, Curtis Gemmell, Sue Lang Source Type: research

Comparative evaluation of different gradient diffusion tests for detection of azole resistance in Aspergillus fumigatus
Gradient diffusion assays Etest and MIC Test Strip for itraconazole, posaconazole and voriconazole as well as isavuconazole (MIC Test Strip) were evaluated for Aspergillus fumigatus against EUCAST broth microdilution. Both assays demonstrated generally good performance; however, posaconazole MIC Test Strip showed low agreement with broth microdilution due to MIC overestimation. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - January 9, 2018 Category: Microbiology Authors: Evgeny A. Idelevich, Uwe Gro ß, Karsten Becker, Oliver Bader Tags: Mycology Source Type: research

Microbiological diagnosis of adult tuberculous meningitis in a ten-year cohort in Indonesia
We evaluated microbiological diagnosis of tuberculous (TB) meningitis in a referral hospital in Indonesia. Over a ten-year period, we examined cerebrospinal fluid (CSF) samples of 1180 adult meningitis suspects. Sensitivity of different methods was compared, and results were stratified for HIV status, disease severity, and CSF volume. TB meningitis was bacteriologically confirmed in 501 patients. Using clinical diagnosis as reference standard (n = 713), sensitivity of different methods was 12.2% (86/703) for microscopy, 42% (73/174) for Xpert MTB/RIF, 46.0% (163/354) for solid culture, 48.8% (332/680) for liquid culture, a...
Source: Diagnostic Microbiology and Infectious Disease - January 9, 2018 Category: Microbiology Authors: Lidya Chaidir, Jessi Annisa, Sofiati Dian, Ida Parwati, Arlisa Alisjahbana, Feby Purnama, Adri van der Zanden, Ahmad Rizal Ganiem, Reinout van Crevel Tags: Mycobacteriology Source Type: research

Evaluation of Sensititre Broth Microdilution Plate for determining the susceptibility of carbapenem-resistant Klebsiella pneumoniae to polymyxins
Colistin and polymyxin B MICs were determined for 106 carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates using Sensititre Research Use Only GNX2F plates (Thermo Fisher) and compared to CLSI broth macrodilution (BMD) as the reference method. For colistin, EUCAST breakpoints were applied and testing of isolates with very major (VM) errors was repeated in duplicate by both methods to determine a majority result. Essential agreement (MIC ± one dilution) of GNX2F with the reference method was 97.1% for polymyxin B and 92.5% for colistin (7 VM errors, 22.6%). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - January 9, 2018 Category: Microbiology Authors: Sandra S. Richter, James Karichu, Joshua Otiso, Hillary Van Heule, George Keller, Eric Cober, Laura J. Rojas, Andrea M. Hujer, Kristine M. Hujer, Steve Marshall, Frederico Perez, Susan D. Rudin, T. Nicholas Domitrovic, Keith S. Kaye, Robert Salata, David Tags: Antimicrobial Susceptibility Study Source Type: research

Microbiological diagnosis of adult tuberculous meningitis in a ten-year cohort in Indonesia
We evaluated microbiological diagnosis of tuberculous (TB) meningitis in a referral hospital in Indonesia. Over a ten-year period, we examined cerebrospinal fluid (CSF) samples of 1180 adult meningitis suspects. Sensitivity of different methods was compared, and results were stratified for HIV status, disease severity, and CSF volume. TB meningitis was bacteriologically confirmed in 501 patients. Using clinical diagnosis as reference standard (n=713), sensitivity of different methods was 12.2% (86/703) for microscopy, 42% (73/174) for Xpert MTB/RIF, 46.0% (163/354) for solid culture, 48.8% (332/680) for liquid culture, and...
Source: Diagnostic Microbiology and Infectious Disease - January 9, 2018 Category: Microbiology Authors: Lidya Chaidir, Jessi Annisa, Sofiati Dian, Ida Parwati, Arlisa Alisjahbana, Feby Purnama, Adri van der Zanden, Ahmad Rizal Ganiem, Reinout van Crevel Source Type: research

Evaluation of Sensititre Broth Microdilution Plate for Determining the Susceptibility of Carbapenem-resistant Klebsiella pneumoniae to Polymyxins
Colistin and polymyxin B MICs were determined for 106 carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates using Sensititre Research Use Only GNX2F plates (Thermo Fisher) and compared to CLSI broth macrodilution (BMD) as the reference method. For colistin, EUCAST breakpoints were applied and testing of isolates with very major (VM) errors was repeated in duplicate by both methods to determine a majority result. Essential agreement (MIC ± one dilution) of GNX2F with the reference method was 97.1% for polymyxin B and 92.5% for colistin (7 VM errors, 22.6%). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - January 9, 2018 Category: Microbiology Authors: Sandra S. Richter, James Karichu, Joshua Otiso, Hillary Van Heule, George Keller, Eric Cober, Laura J. Rojas, Andrea M. Hujer, Kristine M. Hujer, Steve Marshall, Frederico Perez, Susan D. Rudin, T. Nicholas Domitrovic, Keith S. Kaye, Robert Salata, David Source Type: research

Comparison of two commercial quantitative PCR assays and correlation with the first WHO International Standard for human CMV
Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard). Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP or MagNA Pure LC System followed by the kPCR PLX ™ CMV DNA (kPCR) or the CMV R-gene™ assay (R-gene), respectively; 139 clinical specimens were tested. Both assays correlated well with the standard (R2> 0.96) and a matrix effect was observed. Quantitative results correlated reasonably between both assays for whole blood (R2 = 0.79) and well for other specimen types (R2 = 0.93). (Source: Diagnostic Microb...
Source: Diagnostic Microbiology and Infectious Disease - January 7, 2018 Category: Microbiology Authors: Ilka Engelmann, Enagnon Kazali Alidjinou, Mouna Lazrek, Judith Ogiez, Jean-Marie Pouillaude, Emmanuel Chazard, Anny Dewilde, Didier Hober Tags: Virology Source Type: research

Comparison of two commercial quantitative PCR assays and correlation with the first WHO International Standard for human CMV
Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard).Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP or MagNA Pure LC System followed by the kPCR PLXTM CMV DNA (kPCR) or the CMV R-geneTM assay (R-gene), respectively. 139 clinical specimens were tested. Both assays correlated well with the standard (R2>0.96) and a matrix effect was observed. Quantitative results correlated reasonably between both assays for whole blood (R2= 0.79) and well for other specimen types (R2= 0.93). (Source: Diagnostic Microbiology ...
Source: Diagnostic Microbiology and Infectious Disease - January 7, 2018 Category: Microbiology Authors: Ilka Engelmann, Enagnon Kazali Alidjinou, Mouna Lazrek, Judith Ogiez, Jean-Marie Pouillaude, Emmanuel Chazard, Anny Dewilde, Didier Hober Source Type: research

A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test
In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the use of a novel immunochemical reagent in the form of the conjugate of original acridinium label (AL) attached to secondary antibody (IgG-AL) and SAG2-GRA1-ROP1L chimeric antigen for T . (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - January 6, 2018 Category: Microbiology Authors: Lucyna Holec-G ąsior, Bartłomiej Ferra, Justyna Czechowska, Illia E. Serdiuk, Karol Krzymiński Tags: Parasitology Source Type: research

Receiver operating characteristic curve analysis of four Aspergillus-specific IgG assays for the diagnosis of chronic pulmonary aspergillosis
Measurement of Aspergillus-specific IgG is central to the diagnosis of chronic pulmonary aspergillosis (CPA), but manufacturers ’ guidance on test interpretation is based on unpublished data. We performed the first receiver operating characteristic (ROC) area under the curve (AUC) analysis to identify optimal cut-offs for this test in relation to European controls. Aspergillus-specific IgG levels were measured in sera from British adults with CPA and European healthy controls by ImmunoCAP, Immulite, Serion and Bio-Rad assays. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - January 5, 2018 Category: Microbiology Authors: Iain D. Page, Caroline Baxter, Christophe Hennequin, Malcolm D. Richardson, Erna van Hoeyveld, Albert W. van Toorenenbergen, David W. Denning Tags: Mycology Source Type: research

Automated broth-based systems versus the MYCOTB plate for antimicrobial susceptibility testing of the Mycobacterium tuberculosis complex: challenges in interpretation
We examined categorical agreement between automated mycobacterial susceptibility testing methods (Mycobacterial Growth Indicator Tube [MGIT] 960 System and the VersaTREK Mycobacteria Detection and Susceptibility System) which are based on single critical concentration (CC) “breakpoints” and a commercial microbroth dilution method (Sensititre Mycobacterium tuberculosis MIC Plate [MYCOTB]) which provides an MIC value. Mycobacterium tuberculosis isolates (n=355) were tested against three first-line antimycobacterial agents (ethambutol [EMB], isoniazid [INH], rifampin [RIF]) using the MYCOTB plate and either the MGIT 960 (...
Source: Diagnostic Microbiology and Infectious Disease - January 5, 2018 Category: Microbiology Authors: Isabella W. Martin, Kim Dionne, Sharon M. Deml, Nancy L. Wengenack, Nicole M. Parrish Tags: Mycobacteriology Source Type: research

A nitrocefin disc supplemented with ertapenem for rapid screening of carbapenemase-producing Enterobacteriaceae
Reliable, simple and rapid methods for laboratory detection of carbapenemases are important for an appropriate antibiotic administration. A nitrocefin disc containing ertapenem for rapid screening of carbapenemase production among Enterobacteriaceae is developed in the present study. A total of 87 molecularly-confirmed Enterobacteriaceae including 31 carbapenemase producers and 56 non-carbapenemase producers, were tested with nitrocefin discs supplemented with and without ertapenem (20 μg/disc). (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - January 5, 2018 Category: Microbiology Authors: Yothin Teethaisong, Glyn Hobbs, Ismini Nakouti, Katie Evans, Griangsak Eumkeb Tags: Antimicrobial Susceptibility Study Source Type: research

Receiver operating characteristic curve analysis of four Aspergillus-specific IgG assays for the diagnosis of chronic pulmonary aspergillosis
Measurement of Aspergillus-specific IgG is central to the diagnosis of chronic pulmonary aspergillosis (CPA), but manufacturers ’ guidance on test interpretation is based on unpublished data. We performed the first receiver operating characteristic (ROC) area under the curve (AUC) analysis to identify optimal cut-offs for this test in relation to European controls. Aspergillus-specific IgG levels were measured in sera from British adults with CPA and European healthy controls by ImmunoCAP, Immulite, Serion and Bio-Rad assays. (Source: Diagnostic Microbiology and Infectious Disease)
Source: Diagnostic Microbiology and Infectious Disease - January 5, 2018 Category: Microbiology Authors: Iain D Page, Caroline Baxter, Christophe Hennequin, Malcolm D Richardson, Erna van Hoeyveld, Albert W van Toorenenbergen, David W Denning Source Type: research